42 research outputs found
Mikroszatellita lokuszok evolúciója a görögdinnyében (Citrullus lanatus) a középkor óta; (CT)3 deléció a (CT)26 nSSR-ban.
Görögdinnye (Citrullus lanatus) magleletekből (13. sz.,
Debrecen; 15. sz. Buda; 18. sz. Pannonhalma) DNS-izolálást,
molekuláris (nSSR – nuclear simple sequence repeat; cpDNS –
kloroplasztisz DNS) elemzést és fenotípusos fajtarekonstrukciót
végeztünk 44 mai fajtával való összehasonlításban. Az elemzésben
47 primer-párt teszteltünk, ebből 26 primer-pár bizonyult
hatékonynak a mai fajtákban, amelyek közül csak 16 volt aktív a
középkori mintában. Az aktív primerek alkalmazásával szekvencia
elemzést végeztünk a (CT)26-30 nSSR lokuszon, és a clp-12 cpDNS
lokuszon. Megállapítottuk, hogy a középkori mintában még
megtalálható (CT)3 szakasz a mai fajtában már delécióval kiesett
az elmúlt 600 év során. Továbbá a cpDNS trnV (Jarret et al.,
1997) lokuszán (tRNS-Valin; 299 bp) két szubsztitúciót
azonosítottunk (102.196 és a 102.201 nt.-ben). A (CT)26-30 nSSR
lokusz (196 bp) 122-130 bp szakaszán egy további (CT)4 inverziót
is azonosítottunk, amely (CT)26-30 egyszerű mikroszatellita
lokuszból kialakuló (CT)17-C-(TC)3-T-(CT)5 összetett
mikroszatellita születését igazolja. Vizsgálataink során egy új
retrotranszpozont (Cila-1) azonosítottunk. A középkori minta
fajtarekonstrukciójához elkészítettük a 44 mai fajta morfológiai
dendrogramját 25 fenotípusos bélyeg alapján. | The evolution of water melon (Citrullus lanatus)
microsatellites from the 15th century (Debrecen); 13th (Buda); and
18th century, (Pannonhalma) were analyzed. Microsatellite (nSSR,
nuclear simple sequence repeat) and cpDNA profiles of the aDNA
(ancient DNA) of seed remains were compared to modern water
melon cultivars and landraces. Sixteen primer pairs were applied.
Sequence analysis at the (CT)26 and cpDNA trnV loci revealed a
(CT)3 and Adenin deletions, respectively, form the current water
melon cultivar compared to the medieval sample. Cila-1), a new
LTR retrotansposon has been described. For morphological
reconstruction, a dendrogram produced by SPSS11 based on the
presence versus absence of 24 phenotypic characters were also
analyzed
Morfológiai diverzitás sárgadinnyében (Cucumis melo); egy középkori típus fajtarekonstrukciója
47 mai sárgadinnye tájfajta illetve fajta morfológiai diverzitás
vizsgálatát végeztük el 23 fenotípusos bélyeg alapján, egy
középkori lelet (15. sz. eleje) rekonstrukciójához, valamint az eltet
600 év során végbement mikroevolúciós folyamatok
nyomonkövetésére. A vizsgálatok során felvételezett 47 mai
sárgadinnye fajta kivétel nélkül besorolható volt az Európában
elterjedt három fő terméstípusú csoportba: a cikkelyesen barázdás
Kantalup (cantalupensis), a hálózatos-recés terméshéjú
Retikulatusz (reticulatus), és a simahéjú Inodorusz (inodorus)
csoportba. A párhuzamosan végzett molekuláris vizsgálatok,
valamint az itt közölt morfológiai diagramm segítségével a
középkori minta fajta-típusa meghatározható volt, amely egy
inodorusz típusú, sima héjú, zöld húsú sárgadinnye lehetett,
átmeneti formával a „Hógolyó” és a „Kősárga” tájfajta között. | Morphological diversity of melon (Cucumis melo); phenotype
reconstruction of a medieval sample. Morphological diversity
among 47 melon (Cucumis melo) cultivars and landraces from
Hungarian germplasm collection (ABI, Tápiószele) were analyzed
with an ultimate aim to characterize morphologically cv. Hógolyó,
which showed the closest genetic similarity to a medieval melon
recovered from the 15th century. Cultivars based on fruit
morphology were grouped into the three main types of melon as
reticulatus, cantalupensis and inodorus. Cluster analysis (by
SPSS-11) based on 23 morphological (quantitative and
qualitative) traits recorded revealed an extreme diversity among
accessions, nevertheless cultivars were clustered into main melon
clusters with only two exceptions of inodorus type cv. Zimovka J.
and Afghanistan. Cultivars Sweet ananas and Ezüst ananász; and
two Hungarian landraces Kisteleki and Nagycserkeszi showed
close similarity. Cultivars Hógolyó and Túrkeve of inodorus type
were also grouped in one cluster, which provide insight into the
morphological reconstruction of the medieval melon recovered
from the 15th century. These results also indicate that old
Hungarian landraces could be re-introduced into breeding
programs for broadening genetic base of melon
Microsatellite fingerprinting of grapevine (Vitis vinifera L.) varieties of the Carpathian Basin
Altogether 101 Vitis vinifera L. genotypes were analysed at 6 microsatellite loci (Scu8vv, Scu10vv, VVMD21, VVMD36, ssrVRZAG64, ssrVRZAG79). Ninety-seven were autochthonous accessions of the Carpathian Basin and 4 were international cultivars. The allele composition and sizes obtained with the 6 microsatellite primer pairs were appropriate for discrimination of 95 cultivars. Berry colour-variants of cvs Gohér (Gohér fehér-white and Gohér piros-red), Lisztes (Lisztes fehér and Lisztes piros) as well as the cvs Bakator (Bakator piros and Bakator tüdőszín - light red) were exceptions.
Identification of grapevine cultivars using microsatellite-based DNA barcodes
Microsatellite allele sizes were determined at twelve loci in 115 grape genotypes, including 88 ancient Vitis vinifera L. cultivars indigenous to the Carpathian Basin. Pairwise comparison of the microsatellite data led to the identification of ambiguous nomenclature and established differences between two grapevine cultivars, Leányka and Leányszőlő, previously thought of as identical. The data also disproved erroneous assumptions about the parentage of two additional cultivars, one of which was 'Csabagyöngye' ('Pearl of Csaba'), an economically important grapevine and a renowned genetic resource for grape breeding. The results also pointed to several possible parent-progeny relationships which will be subject to further pedigree studies. The DNA typing information was used to construct a barcode system which was incorporated into the Hungarian Vitis Microsatellite Database for efficient and unambiguous identification of grape genotypes, thereby increasing the precision with which genetic resources are managed in Hungary.
Ripening related processes in strawberry, a nonclimacteric fruit: a short overview
Fruits are essential part of the human diet: they provide vitamins, minerals, antioxidants to the mankind. Physiologically they can be divided into two groups-climacteric and nonclimacteric - depending if they display any respiratory peak and dramatic increase in ethylene biosynthesis or do not. Ethylene is a gaseous hormone playing a very important role in several physiological processes in plants. While climacteric fruits, like apples, bananas, tomatoes, peaches, apricots show increased ethylene biosynthesis and dramatic respiratory peak during their ripening, nonclimacteric fruits, like strawberries, grapes, citrus do not.
The most widely used fruits for studying nonclimacteric ripening are strawberries: several papers are focusing on the identification and characterization of ripening related genes from this plant. Therefore here we attempt to summarize the most important advances in strawberry fruit development, and ripening
The effects of ACS (1-aminocyclopropane-l-carboxylate synthase) gene down regulation on ethylene production and fruit softening in transgenic apple
A detailed examination of the production of ethylene and other ripening parameters during storage period has been undertaken in transgenic apple fruits, where the ethylene biosynthesis was inhibited by antisense ACS (l-aminocyclopropane-l-carboxylate synthase) gene. Data indicate down regulation of ethylene production, softening and spoilage in some transgenic lines. In some cases ethylene production was inhibited for over 90 percent, considerable reduction of softening and spoilage was observed probably due to the reduced activity of cell wall degradable enzymes. ACS activity was also monitored during ripening. The fruits of the best transgenic lines could be stored for minimum 4-5 months longer under 5 °C cold room storage conditions and one month longer at normal room temperature. This molecular approach can provide an alternative way to replace the commonly used and costly atmospheric storage of fruits
Test of the utility of apple retrotransposon insertion patterns for molecular identification of 'Jonathan' somatic mutants
Up until today, apple sport mutants proved to be indistinguishable from each other and their progenitors at the molecular level using random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) marker techniques. This is not surprising, since the genomes of these somatic mutants differ only in one or a few small regions that affect economically important characteristics, such as improved fruit colour, size, or flavour. In most cases, these genome differences are probably caused by retrotransposons which are able to convert their RNA transcripts to DNA with reverse transcriptase enzyme prior to reinsertion, but unable to leave the genome and infect other cells. Retrotransposon insertions can alter the expression of other genes and/or the structure of encoded proteins. The sequence-specific amplified polymorphism (S-SAP) technique is capable of revealing the genetic distribution of retrotransposable elements over the whole genome. The present study used this approach to try to characterize and distinguish 'Jonathan' somatic mutants via fingerprinting, which is an unsolved problem
Production of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNA
Transgenic carnations were produced with a modified mammalian bifunctional enzyme cDNA coding 6-phosphofructo-2- kinaseffructose 2,6-bisphosphatase. Relative activity of this enzyme determines the fructose 2,6-bisphosphate (fru 2,6-P2) cytosolic concentration. This metabolite — as a signal molecule — is one of the carbohydrate metabolism regulators. The regenerated Dianthus chinensis and Dianthus caryophyllus shoots were selected on MS basal medium containing 150 mg/1 kanamycin. Transgene integration was proven by PCR analysis with cDNA specific primers followed by Southern hybridization of DNA isolated from selected green shoots, which survived on kanamycin containing medium, so 3 D. chinensis and 20 D. caryophyllus transgenic plants were produced. Transgene expression were examined by RT-PCR. Transformed and control plants were potted in glasshouse to evaluate the effect of modified fru 2,6-P2 on development, growth and carbohydrate metabolism