Ultrastructural analysis of chromatin in meiosis I plus II of rye (Secale cereale L.)

Scanning electron microscopy (SEM) proves to be an appropriate technique for imaging chromatin organization in meiosis I and II of rye (Secale cereale) down to a resolution of a few nanometers. It could be shown for the first time that organization of basic structural elements (coiled and parallel fibers, chromomeres) changes dramatically during the progression to metaphase I and II. Controlled loosening with proteinase K (after fixation with glutaraldehyde) provides an enhanced insight into chromosome architecture even of highly condensed stages of meiosis. By selective staining with platinum blue, DNA content and distribution can be visualized within compact chromosomes as well as in a complex arrangement of fibers. Chromatin interconnecting threads, which are typically observed in prophase I between homologous and non-homologous chromosomes, stain clearly for DNA. In zygotene transversion of chromatid strands to their homologous counterparts becomes evident. In pachytene segments of synapsed and non-synapsed homologs alternate. At synapsed regions pairing is so intimate that homologous chromosomes form one filament of structural entity. Chiasmata are characterized by chromatid strands which traverse from one homolog to its counterpart. Bivalents are characteristically fused at their telomeric regions. In metaphase I and II there is no structural evidence for primary and secondary constrictions. Copyright (C) 2003 S. Karger AG, Basel

Mutual dependencies : ‘change’ and ‘discourse’

status: publishe

Cultivation and analysis of anthocyanin containing types of potatoes in organic farming regarding cultivability and additional health benefits

In a two year research project a representative spectrum of blue potato varieties were cultivated and tested in detail regarding disease infestation, yield potential and the influence of production systems (organic). Cultivation recommendations for blue potatoes could be deduced from this. Furthermore the varying anthocyanin content as well as the antioxidant capacity of the varieties used was analysed. Varieties with a particularly high content will undergo further tests to show the influence of the manner of preparation (boiling, steaming, frying) and determine their use for the processing industry. The combination of ecologically produced potatoes with „additional health benefits“ arouses the customers interest. The cultivation of high yield blue types can be an alternative to the cultivation of yellow fleshed high yield varieties in organic operating companies

Parceria Embrapa - Forschungszentrum Jülich (IBG-2) programa Labex Europa/Alemanha, em novos métodos não-invasivos aplicados a fenotipagem de plantas.

Página 706 em branco

Risk assessment and mapping of extreme floods in non-dyked communities along the Elbe and Mulde Rivers

International audienceAssessing and mapping damage risk of floods for large river basins is still in its infancy. Damage risk is understood to be the combination of flood hazard and the vulnerability of communities to a flood of a particular return period. Risk is calculated and mapped for two communities in which dykes are not located for flood protection: Meissen on the Elbe River and Döbeln in the Mulde catchment. Different methodologies for the computation of flood depth and inundation extent of varying flood return periods (hazard) are compared. Exposure and relative damage to the flooding (vulnerability) based on land-use coverages of different scale are also compared and discussed. A property asset coverage completes the data requirements for the construction of the risk maps. Recommendations for continued research on risk assessments of large river basins conclude the study

Computing performability for wireless sensor networks

The performability of a wireless sensor network (WSN) can be measured using a range of metrics, including reliability (REL) and expected hop count (EHC). EHC assumes each link has a delay value of 1 and devices have no delay or vice versa, which is not necessarily appropriate for WSNs. This paper generalizes the EHC metric into an expected message delay (EMD) that permits arbitrary delay values for both links and devices. Further, it proposes a method based on Augmented Ordered Multivariate Decision Diagram (OMDD-A) that can be used to compute REL, EHC and EMD for WSN with both device and link failures. Simulation results on various networks show the benefits of the OMDD-A approach

Using multi-valued decision diagram to solve the expected hop count problem

The Expected Hop Count (EHC) of a computer communication network has so far been computed for network models that consider only device or link failure, but not both. We introduce an Augmented Ordered Multi-valued Decision Diagram (OMDD-A) to obtain the EHC of a network in which both devices and links may fail. The OMDD-A approach can compute the EHC of a 2100 grid network with 299 paths, which is unsolvable using existing techniques. We show that OMDD-A generates significantly fewer nodes than the corresponding ordered binary decision diagram, leading to large reductions in processing time

Quality of life as subjective experience: Reframing of perception in patients with colon cancer undergoing radical resection with or without adjuvant chemotherapy

Purpose and background: We examined whether patients with colon cancer undergoing surgery with or without adjuvant chemotherapy change the internal standards on which they base their quality-of-life (QL) estimation, and, if they do so, whether this reframing alters interpretation of QL findings. These questions were addressed within a randomized clinical trial of the Swiss Group for Clinical Cancer Research (SAKK 40/93). Patients and methods: After radical resection of adenocar-cinoma of the colon (pT1-4pN>0M0 and pT3-4pN0M0) and perioperative chemotherapy, patients were randomized to three treatment arms: observation only (A), 5-FU 450 rag/m2plus Levamisol (B), or 5-FU 600 mg/m2 (C). QL was measured by linear analogue self-assessment indicators. Patients estimated their pre-surgery QL both before surgery and retrospectively thereafter, and their pre-adjuvant QL both at the beginning of randomly assigned chemotherapy or observation and retrospectively about two months later. Thereafter, current QL was assessed. Paired t-tests were used to test the hypotheses of no change. Results: Overall, 187 patients with at least one pair of corresponding questionnaires were analyzed. Patients estimated their pre-surgery QL after surgery significantly lower than before and their pre-adjuvant QL under treatment or observation also lower than at the beginning. In the adjuvant phase, in contradiction to our hypothesis, chemotherapy had almost no impact on these changes attributed to reframing. Conventionally assessed changes indicated an improvement in QL. Patients with treatment C reported less improvement in functional performance than those with B or those under observation (P = 0.04). Patients with treatment B indicated a greater worsening in nausea/vomiting than those with C, whereas patients with observation only showed an improvement (P = 0.0009). After adjustment of current QL scores under treatment or observation to patients' retrospective estimation, the treatment effects were diluted but the overall improvement was substantially amplified in most QL indicators. Conclusions: Patients with colon cancer substantially re-frame their perception in estimating QL both under radical resection and under adjuvant chemotherapy or observation. This effect is an integral part of patients' adaptation to disease and treatment. An understanding of this phenomenon is of particular relevance for patient care. Its role in evaluating QL endpoints in clinical trials needs further investigatio