Real-case application of mycorrhizal inoculums on Capsicum annuum L. var. longum cv. Szegedi and Kalocsai

The aim of this study was to test the use of commercially available arbuscular mycorrhiza (AM) inoculant Symbivit, amixture of six species of Glomus spp., in spice pepper field cultivation. The inoculants containing arbuscular mycorrhizal fungi (AMF)was able to establish a symbiosis in the rhizosphere of pepper plants and mycorrhizal inoculation increased fresh and dry weights ofshoots of spice pepper cv. Szegedi and only fresh weight of Kalocsai type. There were no significant differences in the root weightsdue to treatment only in fresh weight of Kalocsai pepper type. Treated plants of both variants exhibited an increase in cumulative cropproduction compared with control non-treated plants and the growth response of pepper was higher for var. Szegedi than var. Kalocsai.Mycorrhizal inoculation had a great positive effect on external hyphal length of AMF also showing differences in that between Kalocsaiand Szegedi variants. The root colonization showed seasonality by treated and non-treated plants. The lowest degree of colonizationwas observed in June in general and colonization percent increased during vegetative development and there was a slight decrease atharvesting. In conclusion, it can be stated that inoculation with Symbivit containing mycorrhizal fungi could be an integral part of spicepepper production

Real-case application of mycorrhizal inoculums on Capsicum annuum L. var. longum cv. Szegedi and Kalocsai

The aim of this study was to test the use of commercially available arbuscular mycorrhiza (AM) inoculant Symbivit, a mixture of six species of Glomus spp., in spice pepper field cultivation. The inoculants containing arbuscular mycorrhizal fungi (AMF) was able to establish a symbiosis in the rhizosphere of pepper plants and mycorrhizal inoculation increased fresh and dry weights of shoots of spice pepper cv. Szegedi and only fresh weight of Kalocsai type. There were no significant differences in the root weights due to treatment only in fresh weight of Kalocsai pepper type. Treated plants of both variants exhibited an increase in cumulative crop production compared with control non-treated plants and the growth response of pepper was higher for var. Szegedi than var. Kalocsai. Mycorrhizal inoculation had a great positive effect on external hyphal length of AMF also showing differences in that between Kalocsai and Szegedi variants. The root colonization showed seasonality by treated and non-treated plants. The lowest degree of colonization was observed in June in general and colonization percent increased during vegetative development and there was a slight decrease at harvesting. In conclusion, it can be stated that inoculation with Symbivit containing mycorrhizal fungi could be an integral part of spice pepper production

Single Mild Traumatic Brain Injury Induces Persistent Disruption of the Blood-Brain Barrier, Neuroinflammation and Cognitive Decline in Hypertensive Rats

Traumatic brain injury (TBI) induces blood-brain barrier (BBB) disruption, which contributes to secondary injury of brain tissue and development of chronic cognitive decline. However, single mild (m)TBI, the most frequent form of brain trauma disrupts the BBB only transiently. We hypothesized, that co-morbid conditions exacerbate persistent BBB disruption after mTBI leading to long term cognitive dysfunction. Since hypertension is the most important cerebrovascular risk factor in populations prone to mild brain trauma, we induced mTBI in normotensive Wistar and spontaneously hypertensive rats (SHR) and we assessed BBB permeability, extravasation of blood-borne substances, neuroinflammation and cognitive function two weeks after trauma. We found that mTBI induced a significant BBB disruption two weeks after trauma in SHRs but not in normotensive Wistar rats, which was associated with a significant accumulation of fibrin and increased neuronal expression of inflammatory cytokines TNFα, IL-1β and IL-6 in the cortex and hippocampus. SHRs showed impaired learning and memory two weeks after mild TBI, whereas cognitive function of normotensive Wistar rats remained intact. Future studies should establish the mechanisms through which hypertension and mild TBI interact to promote persistent BBB disruption, neuroinflammation and cognitive decline to provide neuroprotection and improve cognitive function in patients with mTBI

Short GSM mobile phone exposure does not alter human auditory brainstem response

<p>Abstract</p> <p>Background</p> <p>There are about 1.6 billion GSM cellular phones in use throughout the world today. Numerous papers have reported various biological effects in humans exposed to electromagnetic fields emitted by mobile phones. The aim of the present study was to advance our understanding of potential adverse effects of the GSM mobile phones on the human hearing system.</p> <p>Methods</p> <p>Auditory Brainstem Response (ABR) was recorded with three non-polarizing Ag-AgCl scalp electrodes in thirty young and healthy volunteers (age 18–26 years) with normal hearing. ABR data were collected before, and immediately after a 10 minute exposure to 900 MHz pulsed electromagnetic field (EMF) emitted by a commercial Nokia 6310 mobile phone. Fifteen subjects were exposed to genuine EMF and fifteen to sham EMF in a double blind and counterbalanced order. Possible effects of irradiation was analyzed by comparing the latency of ABR waves I, III and V before and after genuine/sham EMF exposure.</p> <p>Results</p> <p>Paired sample t-test was conducted for statistical analysis. Results revealed no significant differences in the latency of ABR waves I, III and V before and after 10 minutes of genuine/sham EMF exposure.</p> <p>Conclusion</p> <p>The present results suggest that, in our experimental conditions, a single 10 minute exposure of 900 MHz EMF emitted by a commercial mobile phone does not produce measurable immediate effects in the latency of auditory brainstem waves I, III and V.</p

Oxytocin receptor gene polymorphisms are associated with human directed social behavior in dogs (Canis familiaris)

The oxytocin system has a crucial role in human sociality; several results prove that polymorphisms of the oxytocin receptor gene are related to complex social behaviors in humans. Dogs' parallel evolution with humans and their adaptation to the human environment has made them a useful species to model human social interactions. Previous research indicates that dogs are eligible models for behavioral genetic research, as well. Based on these previous findings, our research investigated associations between human directed social behaviors and two newly described (−212AG, 19131AG) and one known (rs8679684) single nucleotide polymorphisms (SNPs) in the regulatory regions (5′ and 3′ UTR) of the oxytocin receptor gene in German Shepherd (N = 104) and Border Collie (N = 103) dogs. Dogs' behavior traits have been estimated in a newly developed test series consisting of five episodes: Greeting by a stranger, Separation from the owner, Problem solving, Threatening approach, Hiding of the owner. Buccal samples were collected and DNA was isolated using standard protocols. SNPs in the 3′ and 5′ UTR regions were analyzed by polymerase chain reaction based techniques followed by subsequent electrophoresis analysis. The gene–behavior association analysis suggests that oxytocin receptor gene polymorphisms have an impact in both breeds on (i) proximity seeking towards an unfamiliar person, as well as their owner, and on (ii) how friendly dogs behave towards strangers, although the mediating molecular regulatory mechanisms are yet unknown. Based on these results, we conclude that similarly to humans, the social behavior of dogs towards humans is influenced by the oxytocin system