Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis

Despite the promising value of miRNAs in the diagnostic and prognostic of cardiovascular disease (CVD), recent meta-analyses did not support their potential. Methodological variances in studies may interfere with miRNA profle and afect their results. This study determines if the blood starting material is a source of variance in miRNA profle by performing a paired comparison in plasma and serum of the expression of primary miRNAs associated with CVD. Circulating miRNA yield was similar in both plasma and serum, although a signifcant increase was observed in patients with Non-ST-elevation myocardial infarction (NSTEMI) compared to control volunteers. When normalized by the expression of miR-484, diferent patterns of miRNA expression between serum and plasma. Although NSTEMI modifed the expression of miR-1 and miR-208 in both serum and plasma, plasma displayed a higher variance than serum (Levene's test p<0.01). For miR-133a and miR-26a, diferences were only detected in serum (p=0.0240), and conversely, miR-499a showed diferences only in plasma of NSTEMI (p=0.001). Interestingly, miR-21 showed an opposite pattern of expression, being increased in serum (2−ΔΔCt:5.7, p=0.0221) and decreased in plasma (2−ΔΔCt: 0.5, p=0.0107). Plasma and serum exhibit diferent patterns of circulating miRNA expression in NSTEMI and suggest that results from studies with diferent starting material could not be comparable

Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis

Despite the promising value of miRNAs in the diagnostic and prognostic of cardiovascular disease (CVD), recent meta-analyses did not support their potential. Methodological variances in studies may interfere with miRNA profle and afect their results. This study determines if the blood starting material is a source of variance in miRNA profle by performing a paired comparison in plasma and serum of the expression of primary miRNAs associated with CVD. Circulating miRNA yield was similar in both plasma and serum, although a signifcant increase was observed in patients with Non-ST-elevation myocardial infarction (NSTEMI) compared to control volunteers. When normalized by the expression of miR-484, diferent patterns of miRNA expression between serum and plasma. Although NSTEMI modifed the expression of miR-1 and miR-208 in both serum and plasma, plasma displayed a higher variance than serum (Levene's test p<0.01). For miR-133a and miR-26a, diferences were only detected in serum (p=0.0240), and conversely, miR-499a showed diferences only in plasma of NSTEMI (p=0.001). Interestingly, miR-21 showed an opposite pattern of expression, being increased in serum (2−ΔΔCt : 5.7, p=0.0221) and decreased in plasma (2−ΔΔCt : 0.5, p=0.0107). Plasma and serum exhibit diferent patterns of circulating miRNA expression in NSTEMI and suggest that results from studies with diferent starting material could not be comparable

Early Holocene ritual complexity in South America: the archaeological record of Lapa do Santo (east-central Brazil)

Early Archaic human skeletal remains found in a burial context in Lapa do Santo in eastcentral Brazil provide a rare glimpse into the lives of hunter-gatherer communities in South America, including their rituals for dealing with the dead. These included the reduction of the body by means of mutilation, defleshing, tooth removal, exposure to fire and possibly cannibalism, followed by the secondary burial of the remains according to strict rules. In a later period, pits were filled with disarticulated bones of a single individual without signs of body manipulation, demonstrating that the region was inhabited by dynamic groups in constant transformation over a period of centuries

Fields and Forests: A Stable Isotope Perspective on the Subsistence Strategies of Past Amazonian Peoples

The subsistence strategies employed by the ancient peoples of the Amazon have been a fundamental and extensively debated topic since the inception of archaeological research in the region. Despite a general disagreement regarding carrying capacity and human development in past Amazonia, the initial subsistence debate converged upon strategies based on manioc cultivation and fish protein and the functional division of the Amazon territory between productive (várzea) and unproductive (terra firme) areas. These early remarks, however, were based mainly on limited ethnographic analogy supported by scarce archaeological evidence, highly compromising the validity of the proposed theories. Only in recent years, with the improvement of archaeological science techniques, researchers were able to give a new direction to understanding subsistence practices in ancient Amazonia. Yet, very little is known about the life ways of past Amazonian peoples. This thesis contributes to the debate by presenting newly generated bone collagen-derived carbon and nitrogen stable isotope data from populations occupying three different Bolivian and Brazilian Amazon areas – the Llanos de Mojos, the Central and Lower Amazon basin – between around 2000 to 500 years BP. This study also compares the generated data with published stable isotope evidence from populations in other areas of the Amazon as well as other Neotropical lowland forests to build a larger picture of human subsistence practices in the Amazon under the known Neotropical context. Interpretations focus on a multiproxy perspective, relying on extensive contextual fauna and plant remains evidence recovered throughout the Amazon. The results show marked differences between the three areas. In the Lower Amazon, the data from the Maracá population indicate diets based on a combination of unknown C3 plants, supplemented by riverine protein and a potential small maize contribution. In the Central Amazon basin, the evidence from the Hatahara site shows a significant contribution of riverine fauna, as well as mixed plant sources including maize and C3 plants. Lastly, the results from Loma Salvatierra and Mendoza in the Llanos de Mojos display an exceptionally high contribution of maize, potentially a staple between 1300 and 1200 BP, and evidence of maize consumption amongst potentially domesticated muscovy duck (Cairina moschata). The combined stable isotope evidence from the Amazon shows that consumption of maize was more of a norm than an exception, providing a substantial dietary contribution even in the typical várzea context of Hatahara. Furthermore, the combined dietary evidence shows a diversity of subsistence strategies in the Amazon, centred on the cultivation of maize, root crops, and potentially several palm species, indicating that there was not a single adaptive strategy nor a single staple crop employed by all populations throughout the Amazon. The findings in this study significantly improve our understanding of human dietary patterns in Amazonia over the last two millennia, particularly regarding maize cultivation and animal management strategies, and are a fundamental stepping-stone for future stable isotope studies in the Amazon and the lowlands of South America.Brazilian National Council for Scientific and Technological Development (CNPq

Isotopical analysis of the diet and subsistence patterns of pre-historical huntergatherer groups from Central Brazil

As pesquisas em ecologia isotópica aplicadas à arqueologia têm se desenvolvido amplamente nas últimas duas décadas em todo o mundo, porém poucos trabalhos direcionados unicamente para essa área foram produzidos no país. Este trabalho tem por objetivo gerar dados para testar dois modelos contrastantes sobre as estratégias de subsistência empregadas por sociedades pré-históricas do Brasil Central, desde o final do Período Pleistoceno (ca. 12.000 anos A.P.) até o final do Holoceno utilizando isótopos estáveis como ferramenta de análise. Para tal foram utilizadas amostras de material ósseo arqueológico humano e faunístico provenientes de duas regiões do Brasil Central: Lagoa Santa e Vale do Peruaçu, Minas Gerais. Deste material foram extraídas amostras de colágeno que foram analisadas em um espectrômetro de massa a fim de obter valores de 13C e 15N. Os resultados obtidos para as populações humanas apontam para uma dieta onívora em ambas, com uma forte tendência ao consumo de recursos vegetais em comparação com as demais espécies analisadas da cadeia trófica. Os indivíduos mais jovens de Lagoa Santa (até 5 anos de idade) apresentaram uma diferença estatística significativa nos valores de 15N quando comparados aos demais indivíduos da mesma região, demonstrando assim uma tendência na população a um prolongamento da amamentação. Foram encontradas também evidências de uma possível alteração climática no Vale do Peruaçu a partir da variação de 15N em Kerodon rupestris no decorrer do Holoceno. O sítio de Santana do Riacho, na região de Lagoa Santa apresentou prováveis indícios da presença de milho com dois indivíduos de Cavia aperea que sinalizaram uma dieta tipicamente consumidora de plantas C4 (média 13C= -12,19 e 15N= 2,63) estatisticamente distinta dos demais indivíduos da amostra, entretanto como o espaço amostral é reduzido mais analises são necessárias para confirmar essa tendência.Research in the field of isotopic ecology applied to archaeology has been in constant development for the past two decades, however only a few studies had been made towards this area in Brazil. This study has the objective of generating data to test two contrasting models regarding the strategies of subsistence used by pre-historical societies of Central Brazil, since the end of the Pleistocene (ca. 12.000 years B.P.) and during the Holocene using stable isotopes as an analytic tool. For such, there had been used faunistic and human archaeological bone samples, from two different regions of Central Brazil: Lagoa Santa and Vale do Peruaçu, Minas Gerais state. From this material collagen samples were extracted and, afterwards, analyzed in a mass spectrometer in order to obtain the 13C and 15N values. The results obtained for both human populations indicate a typically omnivorous diet, with a strong tendency towards vegetable consumption if compared with the other animals used in the trophic web characterization. The young human individuals from Lagoa Santa (up to 5 years old) show a statistically different 15N values if compared to the rest of the human remains from the same population, showing a tendency for extensive breastfeeding age. It was also found evidence of a possible climatic change at Vale do Peruaçu around Middle Holocene demonstrated by 15N variations in Kerodon rupestris. Santana do Riacho site, in the Lagoa Santa region showed probable indications of maize presence, as two Cavia aperea samples yielded typical C4 plant consumption (average 13C= -12.19 and 15N= 2.63) also proving to be statistically different from the other individuals of the same species in the whole sample, on the other hand as the sample size is too small and more analysis are needed in order to confirm this tendency