429 research outputs found
Antinociceptive and Cardiorespiratory Effects of a Single Dose of Dexmedetomidine in Laboratory Mice Subjected to Craniotomy under General Anaesthesia with Isoflurane and Carprofen or Meloxicam
Pain refinement represents an important aspect of animal welfare in laboratory animals. Refining analgesia regimens in mice undergoing craniotomy has been sparsely investigated. Here, we sought to investigate the effect of dexmedetomidine in combination with other analgesic drugs on intraoperative anti-nociceptive effects and cardiorespiratory stability. All mice were anaesthetised with isoflurane and received local lidocaine infiltration at the surgical site. Mice were randomised into treatment groups consisting of either carprofen 5 mg kgâ1 or meloxicam 5 mg kgâ1 with or without dexmedetomidine 0.1 mg kgâ1 administered subcutaneously. Intra-anaesthetic heart rates, breathing rates, isoflurane requirements, and arterial oxygen saturations were continuously monitored. We found that administration of dexmedetomidine significantly improved heart and breathing rate stability during two of four noxious stimuli (skin incision and whisker stimulation) compared to non-dexmedetomidine-treated mice and lowered isoflurane requirements throughout anaesthesia by 5â6%. No significant differences were found between carprofen and meloxicam. These results demonstrate that dexmedetomidine reduces nociception and provides intra-anaesthetic haemodynamic and respiratory stability in mice. In conclusion, the addition of dexmedetomidine to anaesthetic regimes for craniotomy offers a refinement over current practice for laboratory mice
Securing Wireless Communication in Critical Infrastructure: Challenges and Opportunities
Critical infrastructure constitutes the foundation of every society. While
traditionally solely relying on dedicated cable-based communication, this
infrastructure rapidly transforms to highly digitized and interconnected
systems which increasingly rely on wireless communication. Besides providing
tremendous benefits, especially affording the easy, cheap, and flexible
interconnection of a large number of assets spread over larger geographic
areas, wireless communication in critical infrastructure also raises unique
security challenges. Most importantly, the shift from dedicated private wired
networks to heterogeneous wireless communication over public and shared
networks requires significantly more involved security measures. In this paper,
we identify the most relevant challenges resulting from the use of wireless
communication in critical infrastructure and use those to identify a
comprehensive set of promising opportunities to preserve the high security
standards of critical infrastructure even when switching from wired to wireless
communication.Comment: Author's version of a paper accepted for publication in Proceedings
of the 20th EAI International Conference on Mobile and Ubiquitous Systems:
Computing, Networking and Services (MobiQuitous 2023
A Case for Integrated Data Processing in Large-Scale Cyber-Physical Systems
Large-scale cyber-physical systems such as manufacturing lines generate vast amounts of data to guarantee precise control of their machinery. Visions such as the Industrial Internet of Things aim at making this data available also to computation systems outside the lines to increase productivity and product quality. However, rising amounts and complexities of data and control decisions push existing infrastructure for data transmission, storage, and processing to its limits. In this paper, we exemplarily study a fine blanking line which can produce up to 6.2 Gbit/s worth of data to showcase the extreme requirements found in modern manufacturing. We consequently propose integrated data processing which keeps inherently local and small-scale tasks close to the processes while at the same time centralizing tasks relying on more complex decision procedures and remote data sources. Our approach thus allows for both maintaining control of field-level processes and leveraging the benefits of âbig dataâ applications
Anatomical Reconstruction and Functional Imaging Reveal an Ordered Array of Skylight Polarization Detectors in Drosophila
Many insects exploit skylight polarization as a compass cue for orientation and navigation. In the fruit fly, Drosophila melanogaster, photoreceptors R7 and R8 in the dorsal rim area (DRA) of the compound eye are specialized to detect the electric vector (e-vector) of linearly polarized light. These photoreceptors are arranged in stacked pairs with identical fields of view and spectral sensitivities, but mutually orthogonal microvillar orientations. As in larger flies, we found that the microvillar orientation of the distal photoreceptor R7 changes in a fan-like fashion along the DRA. This anatomical arrangement suggests that the DRA constitutes a detector for skylight polarization, in which different e-vectors maximally excite different positions in the array. To test our hypothesis, we measured responses to polarized light of varying e-vector angles in the terminals of R7/8 cells using genetically encoded calcium indicators. Our data confirm a progression of preferred e-vector angles from anterior to posterior in the DRA, and a strict orthogonality between the e-vector preferences of paired R7/8 cells. We observed decreased activity in photoreceptors in response to flashes of light polarized orthogonally to their preferred e-vector angle, suggesting reciprocal inhibition between photoreceptors in the same medullar column, which may serve to increase polarization contrast. Together, our results indicate that the polarization-vision system relies on a spatial map of preferred e-vector angles at the earliest stage of sensory processing
Forty-five minutes of physical activity at school each day? Curricular promotion of physical activity in grades one to four
Aim: According to the US Department of Health and Human Services (2008) and the World Health Organization (2008), regular physical activity is essential to the healthy development of children and contributes to the reduction of chronic diseases throughout their life span. However, coordinated comprehensive national and international programs to promote physical activity and sports participation are lacking. According to the German Sports Federation (2006), physical education (PE) classes at German schools are increasingly being canceled or taught outside the school. "fit for pisa" is a German intervention developed in response to the demand for scientific evaluation of interventions like daily physical education. Its goal is to provide quality management-secured, structured and standardized PE instruction by qualified instructors. Subjects and methods: The "fit for pisa" intervention has been implemented at five primary schools in Göttingen, Germany, since 2003. This daily physical education intervention is based on quality standards. At the five primary schools participating in "fit for pisa," students receive a supplementary 3 class hours of PE/week in addition to the mandatory 2 class hours/week for the entire school term. In other words, the children now receive one 45-min session of PE each school day. Daily physical education is now a part of their mandatory school curriculum. The curriculum content and teaching methods have been continuously developed and optimized based on the state guidelines for Lower Saxony. Results: Teacher training is held at the participating schools once a month to promote standardized implementation and quality management of the intervention program. Additional monthly meetings are held so that the teachers can discuss any problems, deviations or other issues occurring in the classes. Checklists are provided for the teachers and external trainers to document the content and objectives of each lesson. Supervisors evaluate the checklists at regular intervals. Using these instruments, the results of the 4-year intervention program were evaluated at the five participating primary schools. Conclusions: This study provides important data for recommendations to implement additional physical education in primary schools, for the corresponding quality assurance instruments and for the optimization of physical education guidelines for primary schools
Small Latency Variations Do Not Affect Player Performance in First-Person Shooters
In interactive systems high latency affects user performance and experience. This is especially problematic in video games. A large number of studies on this topic investigated the effects of constant, high latency. However, in practice, latency is never constant but varies by up to 100 ms due to variations in processing time and delays added by polling between system components. In a large majority of studies, these variations in latency are neither controlled for nor reported. Thus, it is unclear to which degree small, continuous variations in latency affect user performance. If these unreported variations had a significant impact, this might cast into doubt the findings of some studies. To investigate how latency variation affects player performance and experience in games, we conducted an experiment with 28 participants playing a first-person shooter. Participants played with two levels of base latency (50 ms vs. 150 ms) and variation (0 ms vs. 50 ms). As expected, high base latency significantly reduces player performance and experience. However, we found strong evidence that small variations in latency in the order of 50 ms, do not affect player performance significantly. Thus, our findings mitigate concerns that previous latency studies might have systematically ignored a confounding effect
Securing the legacy of TESS through the care and maintenance of TESS planet ephemerides
Much of the science from the exoplanets detected by the TESS mission relies
on precisely predicted transit times that are needed for many follow-up
characterization studies. We investigate ephemeris deterioration for simulated
TESS planets and find that the ephemerides of 81% of those will have expired
(i.e. 1 mid-transit time uncertainties greater than 30 minutes) one
year after their TESS observations. We verify these results using a sample of
TESS planet candidates as well. In particular, of the simulated planets that
would be recommended as JWST targets by Kempton et al. (2018), 80% will
have mid-transit time uncertainties 30 minutes by the earliest time JWST
would observe them. This rapid deterioration is driven primarily by the
relatively short time baseline of TESS observations. We describe strategies for
maintaining TESS ephemerides fresh through follow-up transit observations. We
find that the longer the baseline between the TESS and the follow-up
observations, the longer the ephemerides stay fresh, and that 51% of simulated
primary mission TESS planets will require space-based observations. The
recently-approved extension to the TESS mission will rescue the ephemerides of
most (though not all) primary mission planets, but the benefits of these new
observations can only be reaped two years after the primary mission
observations. Moreover, the ephemerides of most primary mission TESS planets
(as well as those newly discovered during the extended mission) will again have
expired by the time future facilities such as the ELTs, Ariel and the possible
LUVOIR/OST missions come online, unless maintenance follow-up observations are
obtained.Comment: 16 pages, 10 figures, accepted to AJ; main changes are cross-checking
results against the sample of real TOIs, and addressing the impact of the
TESS extended missio
Ten questions concerning integrating smart buildings into the smart grid
Recent advances in information and communications technology (ICT) have initiated development of a smart electrical grid and smart buildings. Buildings consume a large portion of the total electricity production worldwide, and to fully develop a smart grid they must be integrated with that grid. Buildings can now be âprosumersâ on the grid (both producers and consumers), and the continued growth of distributed renewable energy generation is raising new challenges in terms of grid stability over various time scales. Buildings can contribute to grid stability by managing their overall electrical demand in response to current conditions. Facility managers must balance demand response requests by grid operators with energy needed to maintain smooth building operations. For example, maintaining thermal comfort within an occupied building requires energy and, thus an optimized solution balancing energy use with indoor environmental quality (adequate thermal comfort, lighting, etc.) is needed. Successful integration of buildings and their systems with the grid also requires interoperable data exchange. However, the adoption and integration of newer control and communication technologies into buildings can be problematic with older legacy HVAC and building control systems. Public policy and economic structures have not kept up with the technical developments that have given rise to the budding smart grid, and further developments are needed in both technical and non-technical areas
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p53 Modulation as a Therapeutic Strategy in Gastrointestinal Stromal Tumors
The KIT-inhibitor imatinib mesylate (IM) has greatly improved the treatment of metastatic gastrointestinal stromal tumors (GIST). IM exhibits strong antiproliferative effects but fails to induce sufficient levels of apoptosis resulting in low pathologic complete remission rates and a high rate of secondary progression in the metastatic setting. Upregulation of p53 by MDM2 inhibitors has been shown to induce apoptosis in p53 wildtype tumors. Analyzing a series of 62 mostly untreated, localized and metastatic GIST we detected a low rate (3%) of inactivating p53 mutations, thus providing a rationale for further exploration of p53-directed therapeutic strategies. To this end, we studied nutlin-3, an inhibitor of the p53 antagonist MDM2, and RITA, a putative p53 activator, in GIST cell lines. Nutlin-3 effectively induced p53 at therapeutically relevant levels, which resulted in moderate antiproliferative effects and cell cycle arrest in p53 wildtype GIST cell lines GIST430, GIST48 and GIST48B. P53 reactivation substantially improved the apoptotic response after effective KIT inhibition with sunitinib and 17-AAG in IM-resistant cell lines. The commonly used imatinib-sensitive cell lines GIST882 and GIST-T1 were shown to harbor defective p53 and therefore failed to respond to nutlin-3 treatment. RITA induced p53 in GIST48B, followed by antiproliferative effects and a strong induction of apoptosis. Surprisingly, GIST-T1 was also highly sensitive to RITA despite lacking functional p53. This suggested a more complex, p53-independent mechanism of action for the latter compound. No antagonistic effects from p53-activating drugs were seen with any drug combination. Our data provide first evidence that modulation of the MDM2/p53 pathway may be therapeutically useful to improve the apoptotic response of KIT-inhibitory drugs in the treatment of naĂŻve GIST, with p53 mutation status being a predictive factor of response
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