Structural characterizations and rheological properties of water-soluble polysaccharides from two Tunisian marine algae : Cystoseira compressa and Jania adhaerens

Cystoseira compressa et Jania adhaerens sont deux macro-algues marines de la mer méditerranée bien répandues le long des côtes Tunisiennes et non exploitées. Il ressort de cette étude que ces algues pourraient être utilisées comme une source d’ingrédients fonctionnels d’origine naturelle pour la production de burgers à base de chair de poissons d’eau douce (Barbus barbus). De plus, ces travaux ont conduit à l’obtention d’un fucoïdane (CCF) et un alginate de sodium (CCSA) comme polysaccharides matriciels de C. compressa. CCF (1,05 × 105 g/mol) est un hétérogalactofucane sulfaté (14,65 %) composé d’une chaîne principale de α-(1,3 ; 1,4)-l-Fucp ramifiée (31,84 %) en positions O-4 et O-3 par des monosaccharides terminaux et des chaînes latérales. CCSA (Mw = 1 × 105 g/mol) est composé de 56 % de α-l-GulA (G) et 44 % de β-d-ManA (M) (M/G = 0,77). Ce polyuronide est constitué de 93 % d’homoblocs (FGG = 53 % et FMM = 40 %) et de 6 % d’hétéroblocs (FMG = 3 % et FGM = 3 %). Les analyses rhéologiques et biologiques ont montré que CCF et CCSA présentent un comportement rhéofluidifiant ayant des propriétés de liquide visqueux avec des propriétés antioxydantes. Le polysaccharide issu de J. adhaerens (JSP) est un hétéroxylogalactane de Mw = 8,0 × 105 g/mol et est constitué de résidus disaccharidiques répétitifs de [→3)-β-d-Galp-(1,4)-α-l-Galp-(1→)]n et [(→3)-β-d-Galp-(1,4)-3,6-α-l-AnGalp-(1→)]n substitués principalement en position O-6 des résidus (1,3)-β-d-Galp et en positions O-2 et O-3 des résidus (1,4)-α-l-Galp par des T-β-d-Xylp, de groupements sulfate et/ou méthoxyle. Ce galactane sulfaté possède un comportement pseudoplastique typique d’un fluide viscoélastique, ayant des propriétés de gel faible.Cystoseira compressa and Jania adhaerens are two seaweeds widespread on the Tunisian coasts and were not exploited. They have been used as natural ingredients to produce new canned fish burgers prepared from minced fish of common barbel (Barbus barbus). In addition, this work led to the extraction of a fucoidan (CCF) and a sodium alginate (CCSA) as matrix polysaccharides of C. compressa. CCF (Mw=1.05 × 105 g/mol) was a sulfated (14.65%) heterogalactofucan composed of α-(1,3) and α-(1,4)-linked l-Fucp as main backbone, which could be branched (31.8%) in O-3 and O-4 positions by terminal monosaccharides and side chains. CCSA (Mw = 1 × 105 g/mol) was composed of 56% α-l-GulA (G) and 44% β-d-ManA (M) (M/G = 0.77). The CCSA linear backbone was constituted by 93% of homoblocks (FGG = 53% and FMM = 40%) and 6% of heteroblocks (FMG = 3% and FGM = 3%). Rheological and biological investigations showed that CCF and CCSA solutions exhibited shear-thinning and fluid-like viscoelastic behaviors with antioxidant properties. A sulfated xylogalactan-rich fraction (JSP) was extracted from J. adhaerens. JSP (Mw = 8.0 × 105 g/mol) was mainly constituted by the agaran disaccharidic repeating residues (→3)-β-d-Galp-(1,4)-α-l-Galp-(1→)n and (→3)-β-d-Galp-(1,4)-3,6-α-l-AnGalp-(1→)n mainly substituted on O-6 of (1,3)-β-d-Galp residues and in O-2 and O-3 positions of (1,4)-α-l-Galp residues by T-β-d-Xylp, methoxy and/or sulfate groups. JSP solutions displayed a shear-thinning behavior with a great viscoelasticity character, having weak gel properties

Caractérisations structurales et propriétés rhéologiques des polysaccharides de deux algues marines tunisiennes : Cystoseira compressa et Jania adhaerens

Cystoseira compressa and Jania adhaerens are two seaweeds widespread on the Tunisian coasts and were not exploited. They have been used as natural ingredients to produce new canned fish burgers prepared from minced fish of common barbel (Barbus barbus). In addition, this work led to the extraction of a fucoidan (CCF) and a sodium alginate (CCSA) as matrix polysaccharides of C. compressa. CCF (Mw=1.05 × 105 g/mol) was a sulfated (14.65%) heterogalactofucan composed of α-(1,3) and α-(1,4)-linked l-Fucp as main backbone, which could be branched (31.8%) in O-3 and O-4 positions by terminal monosaccharides and side chains. CCSA (Mw = 1 × 105 g/mol) was composed of 56% α-l-GulA (G) and 44% β-d-ManA (M) (M/G = 0.77). The CCSA linear backbone was constituted by 93% of homoblocks (FGG = 53% and FMM = 40%) and 6% of heteroblocks (FMG = 3% and FGM = 3%). Rheological and biological investigations showed that CCF and CCSA solutions exhibited shear-thinning and fluid-like viscoelastic behaviors with antioxidant properties. A sulfated xylogalactan-rich fraction (JSP) was extracted from J. adhaerens. JSP (Mw = 8.0 × 105 g/mol) was mainly constituted by the agaran disaccharidic repeating residues (→3)-β-d-Galp-(1,4)-α-l-Galp-(1→)n and (→3)-β-d-Galp-(1,4)-3,6-α-l-AnGalp-(1→)n mainly substituted on O-6 of (1,3)-β-d-Galp residues and in O-2 and O-3 positions of (1,4)-α-l-Galp residues by T-β-d-Xylp, methoxy and/or sulfate groups. JSP solutions displayed a shear-thinning behavior with a great viscoelasticity character, having weak gel properties.Cystoseira compressa et Jania adhaerens sont deux macro-algues marines de la mer méditerranée bien répandues le long des côtes Tunisiennes et non exploitées. Il ressort de cette étude que ces algues pourraient être utilisées comme une source d’ingrédients fonctionnels d’origine naturelle pour la production de burgers à base de chair de poissons d’eau douce (Barbus barbus). De plus, ces travaux ont conduit à l’obtention d’un fucoïdane (CCF) et un alginate de sodium (CCSA) comme polysaccharides matriciels de C. compressa. CCF (1,05 × 105 g/mol) est un hétérogalactofucane sulfaté (14,65 %) composé d’une chaîne principale de α-(1,3 ; 1,4)-l-Fucp ramifiée (31,84 %) en positions O-4 et O-3 par des monosaccharides terminaux et des chaînes latérales. CCSA (Mw = 1 × 105 g/mol) est composé de 56 % de α-l-GulA (G) et 44 % de β-d-ManA (M) (M/G = 0,77). Ce polyuronide est constitué de 93 % d’homoblocs (FGG = 53 % et FMM = 40 %) et de 6 % d’hétéroblocs (FMG = 3 % et FGM = 3 %). Les analyses rhéologiques et biologiques ont montré que CCF et CCSA présentent un comportement rhéofluidifiant ayant des propriétés de liquide visqueux avec des propriétés antioxydantes. Le polysaccharide issu de J. adhaerens (JSP) est un hétéroxylogalactane de Mw = 8,0 × 105 g/mol et est constitué de résidus disaccharidiques répétitifs de [→3)-β-d-Galp-(1,4)-α-l-Galp-(1→)]n et [(→3)-β-d-Galp-(1,4)-3,6-α-l-AnGalp-(1→)]n substitués principalement en position O-6 des résidus (1,3)-β-d-Galp et en positions O-2 et O-3 des résidus (1,4)-α-l-Galp par des T-β-d-Xylp, de groupements sulfate et/ou méthoxyle. Ce galactane sulfaté possède un comportement pseudoplastique typique d’un fluide viscoélastique, ayant des propriétés de gel faible

Microalgae: A Promising Source of Bioactive Phycobiliproteins

Phycobiliproteins are photosynthetic light-harvesting pigments isolated from microalgae with fluorescent, colorimetric and biological properties, making them a potential commodity in the pharmaceutical, cosmetic and food industries. Hence, improving their metabolic yield is of great interest. In this regard, the present review aimed, first, to provide a detailed and thorough overview of the optimization of culture media elements, as well as various physical parameters, to improve the large-scale manufacturing of such bioactive molecules. The second section of the review offers systematic, deep and detailed data about the current main features of phycobiliproteins. In the ultimate section, the health and nutritional claims related to these bioactive pigments, explaining their noticeable potential for biotechnological uses in various fields, are examined

Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities

International audienc

Novel Antioxidant, Anti-α-Amylase, Anti-Inflammatory and Antinociceptive Water-Soluble Polysaccharides from the Aerial Part of Nitraria retusa

International audienceIn this paper, water-soluble polysaccharides (named as NRLP) were extracted from Nitraria retusa leaves. The main structural features of NRLP were determined by High-pressure size exclusion chromatography, Fourier transform infrared and Gas Chromatography/Mass Spectrometry-Electronic Impact analysis. The in vitro and in vivo biological potential of NRLP were evaluated by measuring its antioxidant (•OH and DPPH• scavenging, total antioxidant capacity), anti-α-amylase as well as anti-inflammatory and antinociceptive activities in a mice model. NRLP was composed of Rha (33.7%), Gal (18.1%), GalA (15.0%), Glc (13.3%), Ara (13.3%), Xyl (3.8%), and GlcA (2.8%) and showed a Molecular Weight (Mw) of 23.0 kDa and a polydispersity index (PDI) of 1.66. The investigations highlighted a significant antioxidant activity (IC50 = 2.4–2.6 mg/mL) and an inhibition activity against α-amylase (IC50 = 4.55 mg/mL) in a dose-dependent manner. Further, NRLP revealed interesting anti-edematous effects and antinociceptive activities (both > 70%). These results open up new pharmacological prospects for the water-soluble polysaccharides extracted from Nitraria retusa leaves

Rheological investigations of water-soluble polysaccharides from the Tunisian brown seaweed Cystoseira compressa

International audienc

Optimal cultivation towards enhanced biomass and floridean starch production by Porphyridium marinum

International audienc

Bioactive carbohydrate polymers: Between myth and reality

Polysaccharides are complex macromolecules long regarded as energetic storage resources or as components of plant and fungal cell walls. They have also been described as plant mucilages or microbial exopolysaccharides. The development of glycosciences has led to a partial and difficult deciphering of their other biological functions in living organisms. The objectives of glycobiochemistry and glycobiology are currently to correlate some structural features of polysaccharides with some biological responses in the producing organisms or in another one. In this context, the literature focusing on bioactive polysaccharides has increased exponentially during the last two decades, being sometimes very optimistic for some new applications of bioactive polysaccharides, notably in the medical field. Therefore, this review aims to examine bioactive polysaccharide, taking a critical look of the different biological activities reported by authors and the reality of the market. It focuses also on the chemical, biochemical, enzymatic, and physical modifications of these biopolymers to optimize their potential as bioactive agents.info:eu-repo/semantics/publishedVersio

Production, extraction and characterization of alginates from seaweeds

International audienc

Structural characterization and antioxidant activity of water-soluble polysaccharides from the Tunisian brown seaweed Cystoseira compressa

International audienceA fucoidan (CCF) and a sodium alginate (CCSA) were extracted and purified from the Tunisian brown seaweed Cystoseira compressa. CCF was a highly sulfated heterogalactofucan composed of α-(1→3), α-(1→4)-linked L-Fucp as main backbone which could be highly branched (31.84%) at O-3 and O-4 positions of α-(1→4)-L-Fucp and α-(1→3)-L-Fucp by terminal monosaccharides and side chains such as terminal α-L-Fucp, terminal β-D-Galp, β-D-Galp-(1→3)-α-L-Fucp and β-D-Galp-(1→4)-α-L-Fucp. The ratio of α-(1→3)/α-(1→4) linkages was estimated at 3.86:1. CCSA was characterized by HPAEC-PAD, GC/MS-EI, ATR-FTIR, and 1H-NMR. The M/G ratio was M/G = 0.77, indicating that CCSA respectively contained 44% and 56% of mannuronic and guluronic acids. The values of FGG, FMM, FGM (or FMG) blocks as well as the parameter η were estimated. The two polysaccharides exhibited effective antioxidant activities by ferrous ion chelation, ferric ion reduction and DPPH radical-scavenging, outlining their potentials as natural additives