2,171 research outputs found
Charged particle tracks in polymers number 6 - A method for charge determination of heavy, multicharged cosmic ray particles
Charge determination of heavy, multicharged cosmic ray particles from particle tracks in cellulose nitrate nuclear emulsion stack
DEVELOPMENT OF VIRTUAL EVENT MARKETING
In the last few years the Pichia pastoris expression system has been gaining more and more interest for the expression of recombinant proteins. Many groups have employed fermentation technology in their investigations because the system is fairly easy to scale up and suitable for the production in the milligram to gram range. A large number of heterologous proteins from different sources has been expressed, but the fermentation process technology has been investigated to a lesser extent. A large number of fermentations are carried out in standard bioreactors that may be insufficiently equipped to meet the demands of high-cell-density fermentations of methylotrophic yeasts. In particular, the lack of on-line methanol analysis leads to fermentation protocols that may impair the optimal expression of the desired products. We have used a commercially available methanol sensor to investigate in detail the effects of supplementary glycerol feeding while maintaining a constant methanol concentration during the induction of a Mut+ strain of Pichia pastoris. Specific glycerol feed rates in the range of 38-4.2 mg × g(exp -1) × h(exp -1) (mg glycerol per gram fresh weight per hour) were investigated. Expression of the recombinant scFv antibody fragment was only observed at specific feed rates below 6 mg × g(exp -1) × h(exp -1). At low specific feed rates, growth was even lower than with methanol as the sole carbon source and the harvest expression level of the scFv was only half of that found in the control fermentation. These results show that glycerol inhibits expression driven by the AOX1 promoter even at extremely limited availability and demonstrate the benefits of on-line methanol control in Pichia fermentation research
Charged particle tracks in polymers - No. 3 - Range and energy loss tables
Range and energy loss for ions tabulated from charged particle tracks in polymer
Using a task-based approach in evaluating the usability of BoBIs in an e-book environment
This paper reports on a usability evaluation of BoBIs (Back-of-the-book Indexes) as searching and browsing tools in an e-book environment. This study employed a task-based approach and within-subject design. The retrieval performance of a BoBI was compared with a ToC and Full-Text Search tool in terms of their respective effectiveness and efficiency for finding information in e-books. The results demonstrated that a BoBI was significantly more efficient (faster) and useful compared to a ToC or Full-Text Search tool for finding information in an e-book environment
LET spectra measurements of charged particles in the P0006 experiment on LDEF
Measurements are under way of the charged particle radiation environment of the Long Duration Exposure Facility (LDEF) satellite using stacks of plastic nuclear track detectors (PNTD's) placed in different locations of the satellite. In the initial work the charge, energy, and linear energy transfer (LET) spectra of charged particles were measured with CR-39 double layer PNTD's located on the west side of the satellite (Experiment P0006). Primary and secondary stopping heavy ions were measured separately from the more energetic particles. Both trapped and galactic cosmic ray (GCR) particles are included, with the latter component being dominated by relativistic iron particles. The results from the P0006 experiment will be compared with similar measurements in other locations on LDEF with different orientation and shielding conditions. The remarkably detailed investigation of the charged particle radiation environment of the LDEF satellite will lead to a better understanding of the radiation environment of the Space Station Freedom. It will enable more accurate prediction of single event upsets (SEU's) in microelectronics and, especially, more accurate assessment of the risk - contributed by different components of the radiation field (GCR's, trapped protons, secondaries and heavy recoils, etc.) - to the health and safety of crew members
Corporal diagnostic work and diagnostic spaces: Clinicians' use of space and bodies during diagnosis
© 2015 The Authors. Sociology of Health & Illness © 2015 Foundation for the Sociology of Health & Illness/John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.An emerging body of literature in sociology has demonstrated that diagnosis is a useful focal point for understanding the social dimensions of health and illness. This article contributes to this work by drawing attention to the relationship between diagnostic spaces and the way in which clinicians use their own bodies during the diagnostic process. As a case study, we draw upon fieldwork conducted with a multidisciplinary clinical team providing deep brain stimulation (DBS) to treat children with a movement disorder called dystonia. Interviews were conducted with team members and diagnostic examinations were observed. We illustrate that clinicians use communicative body work and verbal communication to transform a material terrain into diagnostic space, and we illustrate how this diagnostic space configures forms of embodied 'sensing-and-acting' within. We argue that a 'diagnosis' can be conceptualised as emerging from an interaction in which space, the clinician-body, and the patient-body (or body-part) mutually configure one another. By conceptualising diagnosis in this way, this article draws attention to the corporal bases of diagnostic power and counters Cartesian-like accounts of clinical work in which the patient-body is objectified by a disembodied medical discourse.The Wellcome Trust (Wellcome Trust Biomedical Strategic Award 086034
Measurement of trapped proton fluences in main stack of P0006 experiment
We have measured directional distribution and Eastward directed mission fluence of trapped protons at two different energies with plastic nuclear track detectors (CR-39 with DOP) in the main stack of the P0006 experiment on LDEF. Results show arriving directions of trapped protons have very high anisotropy with most protons arriving from the West direction. Selecting these particles we have determined the mission fluence of Eastward directed trapped protons. We found experimental fluences are slightly higher than results of the model calculations of Armstrong and Colborn
Coherent X-ray Diffractive Imaging; applications and limitations
The inversion of a diffraction pattern offers aberration-free
diffraction-limited 3D images without the resolution and depth-of-field
limitations of lens-based tomographic systems, the only limitation being
radiation damage. We review our experimental results, discuss the fundamental
limits of this technique and future plans.Comment: 7 pages, 8 figure
Canine NAPEPLD-associated models of human myelin disorders
Canine leukoencephalomyelopathy (LEMP) is a juvenile-onset neurodegenerative disorder of the CNS white matter currently described in Rottweiler and Leonberger dogs. Genome-wide association study (GWAS) allowed us to map LEMP in a Leonberger cohort to dog chromosome 18. Subsequent whole genome re-sequencing of a Leonberger case enabled the identification of a single private homozygous non-synonymous missense variant located in the highly conserved metallo-beta-lactamase domain of the N-acyl phosphatidylethanolamine phospholipase D (NAPEPLD) gene, encoding an enzyme of the endocannabinoid system. We then sequenced this gene in LEMP-affected Rottweilers and identified a different frameshift variant, which is predicted to replace the C-terminal metallo-beta-lactamase domain of the wild type protein. Haplotype analysis of SNP array genotypes revealed that the frameshift variant was present in diverse haplotypes in Rottweilers, and also in Great Danes, indicating an old origin of this second NAPEPLD variant. The identification of different NAPEPLD variants in dog breeds affected by leukoencephalopathies with heterogeneous pathological features, implicates the NAPEPLD enzyme as important in myelin homeostasis, and suggests a novel candidate gene for myelination disorders in people
Spectral and polarimetric characterization of the Gas Pixel Detector filled with dimethyl ether
The Gas Pixel Detector belongs to the very limited class of gas detectors
optimized for the measurement of X-ray polarization in the emission of
astrophysical sources. The choice of the mixture in which X-ray photons are
absorbed and photoelectrons propagate, deeply affects both the energy range of
the instrument and its performance in terms of gain, track dimension and
ultimately, polarimetric sensitivity. Here we present the characterization of
the Gas Pixel Detector with a 1 cm thick cell filled with dimethyl ether (DME)
at 0.79 atm, selected among other mixtures for the very low diffusion
coefficient. Almost completely polarized and monochromatic photons were
produced at the calibration facility built at INAF/IASF-Rome exploiting Bragg
diffraction at nearly 45 degrees. For the first time ever, we measured the
modulation factor and the spectral capabilities of the instrument at energies
as low as 2.0 keV, but also at 2.6 keV, 3.7 keV, 4.0 keV, 5.2 keV and 7.8 keV.
These measurements cover almost completely the energy range of the instrument
and allows to compare the sensitivity achieved with that of the standard
mixture, composed of helium and DME.Comment: 20 pages, 11 figures, 5 tables. Accepted for publication by NIM
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