Growth Optimization of Thermophilic Bacteria Bacillus thermoamylovorans and Brevibacillus sp. in Producing Keratinolytic Enzyme

Some thermophilic bacteria have been isolated from Tanjung Sakti hot spring. Two isolates which qualitatively showed keratinolytic activity, identified as Bacillus thermoamylovorans and Brevibacillus sp., were optimized the growth in producing keratinase enzyme. Keratinase is a group of proteolytic enzyme which able to hydrolyze insoluble protein (keratin). In this research, whole chicken feathers were used as keratin substrate. The growth curves and measurement of keratinase enzyme activities were determined to the isolates, with a span of measuring 4 hours during 48 hours incubation, and gained the growth and optimum enzyme activity at 28 h incubation. The isolates were then observed the growth and keratinase activity at several different temperatures and pH. The results showed that the optimum temperature and pH for both isolates were same, i.e. at temperature 70° C and pH 7. The effect of adding a source of carbon and nitrogen into the medium were also observed. The growths of both isolates were increased on the addition of 1% glucose and 0.4% casein

KONSTRUKSI MAHASISWA PROGRAM STUDI S1 PPKN UNIVERSITAS NEGERI SURABAYA TENTANG PNDIDIKAN MULTIKULTUR

Abstrak Penelitian ini bertujuan untuk mengetahui konstruksi mahasiswa prodi S1 PPKn tentang pendidikan multikultur. Penelitian ini menggunakan pendekatan kualitatif dengan desain penelitian fenomenologi. Informan penelitian ini adalah mahasiswa prodi S1 PPKn angkatan 2011 semester 6 (enam) dan angkatan 2010 semester 8 (delapan) yang sudah menempuh mata kuliah pendidikan multikultur. Teknik pengumpulan data menggunakan observasi, dan wawancara mendalam. Analisis data dalam penelitian menggunakan reduksi data, penyajian data, dan penarikan kesimpulan. Hasil penelitian yang diperoleh data mahasiswa PPKn memahami pendidikan multikultur sebagai pendidikan yang mengajarkan tentang berbagai macam perbedaan serta bertujuan menanamkan sikap toleransi yang tinggi terhadap keberagaman. Mahasiswa PPkn meyakini bahwa pendidikan multikultur sangat penting dan sesuai diajarkan di prodi PPKn. Praktik kehidupan multikultural mahasiswa diwujudkan dengan sikap saling menghargai dan menghormati antar sesama mahasiswa. Adanya berbagai perbedaan dan kemajemukan tidak menjadikan alasan bagi mereka untuk saling membeda-bedakan dan menjatuhkan satu dengan lain. Hasil penelitian yang telah dianaalisis menyimpulan bahwa, konstruksi mahasiswa program studi S1 PPKn tentang Pendidikan Multikultur masih belum utuh, terbukti dengan ketidakmampuan mahasiswa PPKn dalam menjelaskan makna Pendidikan Multikultur. Kata Kunci: Konstruksi, Mahasiswa, Pendidikan Multikultural Abstract This research aim to to know student construction of prodi S1 PPKN about education of multikultur. This research use approach qualitative with desain research of fenomenologi. Research informan is student of study program S1 PPKn (Civic Education) generation 2011 semester 6 and generation 2010 semester 8 which have gone through eye of multicultural education. Technique data collecting use observation, and circumstantial interview. Data analysis in research use data discount, presentation of data, and withdrawal of conclusion. Result of obtained by research is students data of PPKn (Civic Education) comprehend education of multikultur as education teaching about is assorted of difference and also aim to inculcate high tolerance attitude to diversity. Students of PPKn (Civic Education) believe that multicultural education is very important and appropriate study program thaugt in Civic Education. The existence of various differences and plurality do not make an excuse for them to discriminate each other and dropping each other. The research that has been analyzed, it can be concluded that the constuction of undergraduate students of PPKn (Civic Education) abaout Muticultural Education as incomplete, as evidenced by the inability of students of PPKn (Civic Education) in explaining the meaning of Multicultural Education. Keyword: Construction, Student, Multicultural Educatio

Efektivitas Ekstrak Buah Beta Vulgaris L. (Buah Bit) Dengan Berbagai Fraksi Pelarut Terhadap Mortalitas Larva Aedes Aegypti

. Dengue vector control is mostly done by using plant-based insecticides. Insecticides from the vegetable and fruit extracts of the leaves of plants that contain compounds alkaloids, saponins, flavonoids, tannins, triterpenoids, and polyphenols can be used as an alternative to naturally control Ae. aegypti. The purpose of this study was to determine the effectiveness of the B. vulgaris L. extract larvacide against larvae of Ae. aegypti. The materials that been used was B. vulgaris L. fruit parts which was milled and dried to become a powder form. 800 g of dry powder was extracted by 70% methanol by percolation method with occasional stirring for 3 days. The extract was concentrated using an evaporator. 60 g remaining residue was dissolved in distilled water and re-extracted with diethyl ether, chloroform, and ethyl acetate. Each fraction extract was dried with anhydrous sodium sulfate and the solvent was distilled. The extract was tested qualitatively to determine the content of secondary metabolites. Larvacide test performed by dissolving each extracts in dimetilsulphoxide (DMSO) at concentrations of 0.1, 0.5 and 1%. The larvae used was larval of Ae. aegypti age of seven days. Death larvae counted every day for seven days to determine the effect of the contact. Tests carried out at a temperature of 27±1° C by immersing 25 larvae at each concentration of the extract with 50 mL volume and three replications was performed. The data obtained were analyzed further with different test. The results showed that fruit extract contains flavonoids, alkoloid, sterols, triterpenes, saponins and tannins. Highest mortality happened which was 82.5% and the lowest mortality happened with a concentration of 0.1% diethyl ether extract fraction. The extracts that are dissolved in various solvent fractions have not been effective as a larvacide until the highest concentration which was 1%. Methanol and polar solvent extracts of the fruit has a larvacide potency a bit higher than other solvents

Pengaruh Budgetary Goal Characteristics terhadap Kinerja Manajerial dengan Kecukupan Anggaran dan Komitmen Organisasi sebagai Variabel Pemoderasi pada Universitas Negeri YOGYAKARTA

Penelitian ini bertujuan untuk mengetahui: (1) Pengaruh Budgetary Goal Characteristics terhadap Kinerja Manajerial. (2) Kecukupan Anggaran dalam memoderasi pengaruh Budgetary Goal Characteristics terhadap Kinerja Manajerial. (3) Komitmen Organisasi dalam memoderasi pengaruh Budgetary Goal Characteristics terhadap Kinerja Manajerial. (4) Kecukupan Anggaran dan Komitmen Organisasi bersama-sama memoderasi pengaruh Budgetary Goal Characteristics terhadap Kinerja Manajerial. Metode analisis data menggunakan uji prasyarat analisis, regresi linear sederhana dan moderating regression analysis (MRA). Hasil penelitian ini menunjukkan: (1) Budgetary Goal Characteristics berpengaruh positif terhadap Kinerja Manajerial, ditunjukkan oleh nilai koefisien sebesar 0,481, signifikansi 0,000, dan nilai thitung 5,018. (2) Kecukupan Anggaran memperkuat pengaruh positif Budgetary Goal Characteristics terhadap Kinerja Manajerial. Interaksi dengan variabel pemoderasi menghasilkan nilai koefisien 0,361, signifikansi 0,000, dan nilai thitung 5,177. (3) Komitmen Organisasi tidak memperkuat pengaruh positif Budgetary Goal Characteristics terhadap Kinerja Manajerial. Interaksi variabel pemoderasi mempunyai nilai koefisien 0,063, signifikansi 0,587, dan nilai thitung 0,546. (4) Kecukupan Anggaran dan Komitmen Organisasi bersama-sama memperkuat pengaruh positif Budgetary Goal Characteristics terhadap Kinerja Manajerial. Interaksi dengan variabel pemoderasi menghasilkan nilai koefisien 0,273, signifikansi 0,035, dan nilai thitung 2,151 serta F hitung 12,278

Analisis Kejelasan Dan Kelengkapan Teks Petunjuk Denah Pada Karangan Siswa Kelas VIII Di SMP Negeri 2 Sawit Boyolali

This study aimed to analyze the completeness and clarity of text instructions on the composition sketch eighth grade students at SMP Negeri 2 Sawit. The data used in this study is the composition students of SMP Negeri 2 Sawit. The data collection technique used is the technique of documentation. Data analysis method used is the method agih followed by insertion technique, expansion, and dressing. The results of this study are as follows. (1) Based on the analysis of the completeness of the number of sentences and the completeness of the plan of 15 discourse, there are 11 discourse incomplete and 4 discourse incomplete. (2) Based on an analysis of clarity sentence of 15 discourse, there are two discourses have a clear sentence, 3 discourse in which there is less clear sentences and 10 sentences of discourse in which there are not clear. Keywords: discourse, sentence, completeness and clarity

PENGARUH PENERAPAN TOTAL QUALITY MANAGEMENT TERHADAP KINERJA KEUANGAN DENGAN SISTEM PENGUKURAN KINERJA SEBAGAI VARIABEL MODERASI PADA CV MUTIARA ABADI SEMARANG

The optimal application of TQM must be followed by the application of elements from the management accounting system, one of which is the performance measurement system because it can help the achievement of strategic objectives and short-term goals of the company. This research aims to analyze the effect of implementing Total Quality Management on financial performance with a performance measurement system as a moderating variable on CV Mutiara Abadi Semarang. Data analysis method used is Moderated Regression Analysis to determine the effect of moderating variable. This research shows that Total Quality Management has a significant effect on financial performance, the performance measurement system has no significant effect on financial performance and the performance measurement system does not moderate the relationship between Total Quality Management and financial performance

Analisis Kebutuhan Penyediaan Air Bersih di Kota Palembang: Analysis of Water Demand Supply in Palembang

[ID] Cakupan pelayanan air bersih di Kota Palembang pada tahun 2020 sebesar 83,22%, sehingga masih ada 16,78% penduduk Kota Palembang yang belum dapat terlayani. Kondisi tersebut akan semakin memburuk seiring bertambahnya kebutuhan air tiap tahunnya. Penelitian ini bertujuan untuk mengetahui cakupan pelayanan, kebutuhan air maksimum dan neraca air, sehingga diharapkan dapat menjawab persoalan yang akan dibahas. Penelitian dimulai dengan melakukan proyeksi penduduk dan proyeksi pelanggan. Hasil proyeksi akan menghasilkan proyeksi cakupan pelayanan. Perhitungan kebutuhan air didapatkan dari kebutuhan dari jumlan pelanggan yang telah diproyeksikan. Selanjutnya proyeksi kebutuhan air akan dihubungkan dengan rencana penambahan kapasitas produksi sehingga menghasilkan neraca air. Hasil penelitian menunjukkan bahwa pada tahun 2021 dan tahun selanjutnya diproyeksikan cakupan pelayanan akan mengalami perbaikan dan pada tahun 2030 pelayanan air bersih 100% Kota Palembang dapat tercapai. Kebutuhan air pada tahun 2021 sebesar 4.875 lps dan semakin meningkat pada tahun berikutnya hingga pada tahun 2033 kebutuhan air mencapai 7.146 lps. Kondisi neraca air juga diproyeksikan akan mengalami perbaikan, dimana pada tahun 2020 masih mengalami defisit kapasitas sebesar 257 lps dan pada tahun berikutnya hingga tahun 2031 dapat mempertahankan surplus kapasitas. [EN] The coverage of clean water services in Palembang City in 2020 is 83.22%, so there are still 16.78% of the population of Palembang City who have not been served. This condition will worsen as the demand for water increases every year. This study aims to determine service coverage, maximum water demand and water balance, so that it is expected to be able to answer the issues to be discussed. The research begins by conducting population projections and customer projections. The projection results will produce a projection of service coverage. The calculation of water needs is obtained from the needs of the projected number of customers. Furthermore, the projected water demand will be linked to the plan to increase production capacity so as to produce a water balance. The results of the study show that in 2021 and the following year it is projected that service coverage will improve and in 2030 100% clean water services for Palembang City can be achieved. The need for water in 2021 is 4,875 lps and will increase in the following year until in 2033 the water needs will reach 7,146 lps. The condition of the water balance is also projected to improve, where in 2020 it will still experience a capacity deficit of 257 lps and in the following year until 2031 it can maintain a capacity surplus

Analisis Faktor-faktor Pendorong Pembelian Baju Bekas “Rombengan” Melalui Media Sosial (Facebook) di Kota

Meningkatnya perkembangan internet mengakibatkan terjadinya perubahan- perubahan yang mempengaruhi kehidupan masyarakat, terutama yang berada di kota- kota besar salah satunya di kota Kupang, dimana salah satu perubahan itu adalah tempat berbelanja masyarakat. Mereka tidak lagi hanya berbelanja di toko secara offline tetapi sudah secara online melalui live (siaran) langsung) melalui media social dalam hal ini Facebook. Penjualan pakaian bekas “rombengan” saat ini di Kota Kupang sudah sangat menjamur dan sangat digemari dan fenomena pembelian secara live sangat digemari oleh pembeli. Penelitian ini bertujuan untuk mengetahui faktor-faktor pendorong pembelian baju bekas “rombengan” melalui media sosial (Facebook) di kota Kupang yang diharapkan dapat memberikan dampak positifbagi para pelaku usaha dalam bidang penjualan baju bekas di Kota Kupang maupun penjualan produk lainnya. Teknik pengumpulan data dalam penelitian ini menggunakan wawancara mendalam, observasi dan dokumentasi. Keabsahan data menggunakan triangulasi sumber dan triangulasi metode. Teknik analisis data yaitu pengumpulan data, reduksi data, penyajian data dan penarikan kesimpulan. Hasil penelitian menunjukkan bahwa konsumen pakaian bekas di kota Kupang yaitu Kebutuhan akan pakaian karena alasan pendapatan mereka, pakaian bekas yang dijual bermerek, harga baju bekas yang murah, banyak pilihan pakaian yang tersedia dan kemudahan dalam pembelian