Visible reflectance and content of isomeric ratio of lycopene in commercial and elevated lycopene tomato varieties by different technological traits

Quantification of red pigment content of fruits using destructive techniques is expensive and it enables only the analysis of batches but not of individual items. This study examined the feasibility of using non-destructive, spectrophotometric method to predict one of the most valuable internal quality indices, lycopene, in individual tomato fruits. An open field experiment was conducted to study the effect of irrigation and potassium supplementation on the yield and lycopene content of processed tomato fruit. Three different treatments (regularly irrigated RI, irrigation cut-off 30 days before harvest CO, and rainfed RF unirrigated control) and two different potassium fertilisations (P) were applied. Regular irrigation significantly decreased the lycopene content of tomato fruits. The CO treatment resulted in the highest total lycopene without potassium supplementation. Potassium supplementation, given at the time before fruit maturity, significantly increased the lycopene concentration of cultivar Brigade F1, independently of irrigation. The closest correlation was at 700 nm R2=0.38 and R2=0.45, between reflectance and the (all-E)-lycopene and the (9Z)+(13Z)-lycopene isomers, respectively

Effect of water supply on canopy temperature, stomatal conductance and yield quantity of processing tomato (Lycopersicon esculentum Mill.)

Tomato (Lycopersicon esculentum Mill.) is one of the most extensively cultivated horticultural crops in the world.Water supply is important for yield quantity and quality. The aims of the present study were 1) to evaluate the canopy temperature and the stomatal conductance on processing tomato substances with different water supply, 2) to investigate the effect of different water supply on yield quantity. There were two irrigated treatments, one of them was the reguralry irrigated plant stand which got 333 mm water during investigated period, including the precipitation and the other was the cut off substance which means the irrigation (drip) was stopped at the beginning of the ripening process and there was a control as well which got 189 mm precipitation. The canopy temperature was measured row by row with a Raytek MX 4 type infrared remote thermometer. The stomatal conductance was measured by Delta-T AP4 type porometer. There were significant differences between the control and irrigated plants according to the water supply which was formulated the canopy values. The plants with a deficient water supply were decreased the transpiration rate, therefore its cooling effect didn’t show up. The regularly irrigated tomato plants’ yield exceeded the unirrigated ones more than twice. It is emerged from the study that the irrigation has a positive effect on the amount of the harvestable yield in this year type

Effect of water supply on canopy temperature, stomatal conductance and yield quantity of processing tomato (Lycopersicon esculentum Mill.)

Tomato (Lycopersicon esculentum Mill.) is one of the most extensively cultivated horticultural crops in the world.Water supply isimportant for yield quantity and quality. The aims of the present study were 1) to evaluate the canopy temperature and the stomatal conductanceon processing tomato substances with different water supply, 2) to investigate the effect of different water supply on yield quantity.There were two irrigated treatments, one of them was the reguralry irrigated plant stand which got 333 mm water during investigatedperiod, including the precipitation and the other was the cut off substance which means the irrigation (drip) was stopped at the beginning ofthe ripening process and there was a control as well which got 189 mm precipitation. The canopy temperature was measured row by row witha Raytek MX 4 type infrared remote thermometer. The stomatal conductance was measured by Delta-T AP4 type porometer. There weresignificant differences between the control and irrigated plants according to the water supply which was formulated the canopy values. Theplants with a deficient water supply were decreased the transpiration rate, therefore its cooling effect didn’t show up. The regularly irrigatedtomato plants’ yield exceeded the unirrigated ones more than twice. It is emerged from the study that the irrigation has a positive effect on theamount of the harvestable yield in this year type

Simultaneous impact of the different water supply and year type on processing tomato yield

A two year (2008 and 2009) open field experiment was conducted to study the effect of irrigation on the yield parameters and fruit components of processing tomato. Two different treatments were applied: regularly irrigated (RI), irrigation cut-off 30 days before harvest (CO), compared with unirrigated control (RF). The optimal water supply was calculated from average daily temperature. The aims of the study were to investigate the effect of different water supply on yield quantity. The regularly irrigated plant stands gave significantly higher yield, and unirrigated plants showed yield loss.Water supply had strong positive (R2=0.81) effect on marketable yield and average fruit weight (R2=0.78). Linear regression showed, that 46.5 mm more water supply caused 10 t/ha more marketable yield, and 13.4 mm more water supply caused 1 g more in the average fruit weight. The irrigation increased the Brix yield as well

Transfer of complex regional pain syndrome to mice via human autoantibodies is mediated by interleukin-1–induced mechanisms

Neuroimmune interactions may contribute to severe pain and regional inflammatory and autonomic signs in complex regional pain syndrome (CRPS), a posttraumatic pain disorder. Here, we investigated peripheral and central immune mechanisms in a translational passive transfer trauma mouse model of CRPS. Small plantar skin–muscle incision was performed in female C57BL/6 mice treated daily with purified serum immunoglobulin G (IgG) from patients with longstanding CRPS or healthy volunteers followed by assessment of paw edema, hyperalgesia, inflammation, and central glial activation. CRPS IgG significantly increased and prolonged swelling and induced stable hyperalgesia of the incised paw compared with IgG from healthy controls. After a short-lasting paw inflammatory response in all groups, CRPS IgG-injected mice displayed sustained, profound microglia and astrocyte activation in the dorsal horn of the spinal cord and pain-related brain regions, indicating central sensitization. Genetic deletion of interleukin-1 (IL-1) using IL-1αβ knockout (KO) mice and perioperative IL-1 receptor type 1 (IL-1R1) blockade with the drug anakinra, but not treatment with the glucocorticoid prednisolone, prevented these changes. Anakinra treatment also reversed the established sensitization phenotype when initiated 8 days after incision. Furthermore, with the generation of an IL-1β floxed(fl/fl) mouse line, we demonstrated that CRPS IgG-induced changes are in part mediated by microglia-derived IL-1β, suggesting that both peripheral and central inflammatory mechanisms contribute to the transferred disease phenotype. These results indicate that persistent CRPS is often contributed to by autoantibodies and highlight a potential therapeutic use for clinically licensed antagonists, such as anakinra, to prevent or treat CRPS via blocking IL-1 actions

Novel peptide calcitonin gene-related peptide antagonists for migraine therapy.

Objectives It has previously been shown that the peptide (34Pro,35Phe)CGRP27-37 is a potent calcitonin gene-related peptide, CGRP receptor antagonist, and in this project we aimed to improve the antagonist potency through the structural modification of truncated C-terminal CGRP peptides. Methods Six peptide analogues were synthesized and the anti-CGRP activity confirmed using both in vitro and in vivo studies. Key findings A 10 amino acid-containing peptide VPTDVGPFAF-NH2 (P006) was identified as a key candidate to take forward for in vivo evaluation, where it was shown to be an effective antagonist after intraperitoneal injection into mice. P006 was formulated as a preparation suitable for nasal administration by spray drying with chitosan to form mucoadhesive microcarriers (9.55 ± 0.91 mm diameter) and a loading of 0.2 mg peptide per 20 mg dose.Conclusions The project has demonstrated the potential of these novel small peptide CGRP antagonists, to undergo future preclinical evaluation as anti-migraine therapeutics

Research Recommendations Following the Discovery of Pain Sensitizing IgG Autoantibodies in Fibromyalgia Syndrome

BACKGROUND: Fibromyalgia syndrome (FMS) is the most common chronic widespread pain condition in rheumatology. Until recently, no clear pathophysiological mechanism for fibromyalgia had been established, resulting in management challenges. Recent research has indicated that serum IgGs may play a role in FMS. We undertook a research prioritisation exercise to identify the most pertinent research approaches that may lead to clinically implementable outputs. METHODS: Research priority setting was conducted in five phases: situation analysis; design; expert group consultation; interim recommendations; consultation and revision. A dialogue model was used, and an international multi-stakeholder expert group was invited. Clinical, patient, industry, funder, and scientific expertise was represented throughout. Recommendation-consensus was determined via a voluntary closed eSurvey. Reporting guideline for priority setting of health research were employed to support implementation and maximise impact. RESULTS: Arising from the expert group consultation (n = 29 participants), 39 interim recommendations were defined. A response rate of 81.5% was achieved in the consensus survey. Six recommendations were identified as high priority- and 15 as medium level priority. The recommendations range from aspects of fibromyalgia features that should be considered in future autoantibody research, to specific immunological investigations, suggestions for trial design in FMS, and therapeutic interventions that should be assessed in trials. CONCLUSIONS: By applying the principles of strategic priority setting we directed research towards that which is implementable, thereby expediating the benefit to the FMS patient population. These recommendations are intended for patients, international professionals and grant-giving bodies concerned with research into causes and management of patients with fibromyalgia syndrome