FAK-mediated mechanotransduction in skeletal regeneration

The majority of cells are equipped to detect and decipher physical stimuli, and then react to these stimuli in a cell type-specific manner. Ultimately, these cellular behaviors are synchronized to produce a tissue response, but how this is achieved remains enigmatic. Here, we investigated the genetic basis for mechanotransduction using the bone marrow as a model system. We found that physical stimuli produced a pattern of principal strain that precisely corresponded to the site-specific expression of sox9 and runx2, two transcription factors required for the commitment of stem cells to a skeletogenic lineage, and the arrangement and orientation of newly deposited type I collagen fibrils. To gain insights into the genetic basis for skeletal mechanotransduction we conditionally inactivated focal adhesion kinase (FAK), an intracellular component of the integrin signaling pathway. By doing so we abolished the mechanically induced osteogenic response and thus identified a critical genetic component of the molecular machinery required for mechanotransduction. Our data provide a new framework in which to consider how physical forces and molecular signals are synchronized during the program of skeletal regeneration

extraction from the essence of pure power

This thesis examines the intersections of bodybuilding and performance through a masculine lens. Much like theater, the body builder is activated by staging. In an investigation of its history and a sampling of its theories, we can challenge the hyper-masculine identity which is supported through the gym culture and gender constructs. The arena of the gym contains cues contributing to the artworks listed, and the icons of the gym itself are brought into the studio to transform not only the objects, but the actions themselves. The work challenges the ideals surrounding the superhero, godlike persona cultivated through media tropes. These tropes can be damaging to the fluidity of gender performance as well as the male body image. To diminish ones bodily functionality for the sake of standards made by a dysfunctional culture creates a disparity between its health an appearance. While the strategies problematize the masculine identity, they bring frontally the body as a method of making. The outward, physical struggle between the artist and the object is similar to the internalized struggle of performing male. The task is designed to push the performer to his limits. The performance is based in truth and can be described as theatrical realism

Advanced Gas Turbine (AGT) power-train system development

Technical work on the design and component testing of a 74.5 kW (100 hp) advanced automotive gas turbine is described. Selected component ceramic component design, and procurement were tested. Compressor tests of a modified rotor showed high speed performance improvement over previous rotor designs; efficiency improved by 2.5%, corrected flow by 4.6%, and pressure ratio by 11.6% at 100% speed. The aerodynamic design is completed for both the gasifier and power turbines. Ceramic (silicon carbide) gasifier rotors were spin tested to failure. Improving strengths is indicated by burst speeds and the group of five rotors failed at speeds between 104% and 116% of engine rated speed. The emission results from combustor testing showed NOx levels to be nearly one order of magnitude lower than with previous designs. A one piece ceramic exhaust duct/regenerator seal platform is designed with acceptable low stress levels

Poly(oxime-ester) Vitrimers with Catalyst-Free Bond Exchange.

Vitrimers are network polymers that undergo associative bond exchange reactions in the condensed phase above a threshold temperature, dictated by the exchangeable bonds comprising the vitrimer. For vitrimers, chemistries reliant on poorly nucleophilic bond exchange partners (e.g., hydroxy-functionalized alkanes) or poorly electrophilic exchangeable bonds, catalysts are required to lower the threshold temperature, which is undesirable in that catalyst leaching or deactivation diminishes its influence over time and may compromise reuse. Here we show how to access catalyst-free bond exchange reactions in catalyst-dependent polyester vitrimers by obviating conventional ester bonds in favor of oxime-esters. Poly(oxime-ester) (POE) vitrimers are synthesized using thiol-ene click chemistry, affording high stretchability and malleability. POE vitrimers are readily recycled with little degradation of their initial mechanical properties, suggesting exciting opportunities for sustainable plastics

Closed-loop recycling of plastics enabled by dynamic covalent diketoenamine bonds.

Recycled plastics are low-value commodities due to residual impurities and the degradation of polymer properties with each cycle of re-use. Plastics that undergo reversible polymerization allow high-value monomers to be recovered and re-manufactured into pristine materials, which should incentivize recycling in closed-loop life cycles. However, monomer recovery is often costly, incompatible with complex mixtures and energy-intensive. Here, we show that next-generation plastics-polymerized using dynamic covalent diketoenamine bonds-allow the recovery of monomers from common additives, even in mixed waste streams. Poly(diketoenamine)s 'click' together from a wide variety of triketones and aromatic or aliphatic amines, yielding only water as a by-product. Recovered monomers can be re-manufactured into the same polymer formulation, without loss of performance, as well as other polymer formulations with differentiated properties. The ease with which poly(diketoenamine)s can be manufactured, used, recycled and re-used-without losing value-points to new directions in designing sustainable polymers with minimal environmental impact

Reverse Khas'minskii condition

The aim of this paper is to present and discuss some equivalent characterizations of p-parabolicity in terms of existence of special exhaustion functions. In particular, Khas'minskii in [K] proved that if there exists a 2-superharmonic function k defined outside a compact set such that $\lim_{x\to \infty} k(x)=\infty$, then R is 2-parabolic, and Sario and Nakai in [SN] were able to improve this result by showing that R is 2-parabolic if and only if there exists an Evans potential, i.e. a 2-harmonic function $E:R\setminus K \to \R^+$ with \lim_{x\to \infty} \E(x)=\infty. In this paper, we will prove a reverse Khas'minskii condition valid for any p>1 and discuss the existence of Evans potentials in the nonlinear case.Comment: final version of the article available at http://www.springer.co

Advanced Gas Turbine (AGT): Power-train system development

Technical work on the design and effort leading to the testing of a 74.5 kW (100 hp) automotive gas turbine is described. The general effort was concentrated on building an engine for test starting in July. The buildup progressed with only routine problems and the engine was delivered to the test stand 9 July. In addition to the engine build effort, work continued in selected component areas. Ceramic turbine parts were built and tested. Burst tests of ceramic rotors show strengths are approaching that achieved in test bars; proof testing is required for acceptable strength ceramic vanes. Over 25 hours was accumulated on the combustor rig in three test modes: pilot nozzle only, start nozzle, and main nozzle operation. Satisfactory ignition was achieved for a wide range of starting speeds and the lean blowout limit was as low as 0.06 kg/b (0.14 lb/hr). Lean blowout was more a function of nozzle atomization than fuel/air ratio. A variety of cycle points were tested. Transition from start nozzle flow to main nozzle flow was done manually without difficulty. Regenerator parts were qualification tested without incident and the parts were assembled on schedule. Rig based performance matched first build requirements. Repeated failures in the harmonic drive gearbox during rig testing resulted in that concept being abandoned for an alternate scheme