Het studiejaar 1968/69 : rede uitgesproken op de 7de september 1969 bij het einde van het studiejaar 1968/69

Rede Wageninge

The path inference filter: model-based low-latency map matching of probe vehicle data

We consider the problem of reconstructing vehicle trajectories from sparse sequences of GPS points, for which the sampling interval is between 10 seconds and 2 minutes. We introduce a new class of algorithms, called altogether path inference filter (PIF), that maps GPS data in real time, for a variety of trade-offs and scenarios, and with a high throughput. Numerous prior approaches in map-matching can be shown to be special cases of the path inference filter presented in this article. We present an efficient procedure for automatically training the filter on new data, with or without ground truth observations. The framework is evaluated on a large San Francisco taxi dataset and is shown to improve upon the current state of the art. This filter also provides insights about driving patterns of drivers. The path inference filter has been deployed at an industrial scale inside the Mobile Millennium traffic information system, and is used to map fleets of data in San Francisco, Sacramento, Stockholm and Porto.Comment: Preprint, 23 pages and 23 figure

A nonsense mutation in B3GALNT2 is concordant with hydrocephalus in Friesian horses

Background: Hydrocephalus in Friesian horses is a developmental disorder that often results in stillbirth of affected foals and dystocia in dams. The occurrence is probably related to a founder effect and inbreeding in the population. The aim of our study was to find genomic associations, to investigate the mode of inheritance, to allow a DNA test for hydrocephalus in Friesian horses to be developed. In case of a monogenic inheritance we aimed to identify the causal mutation. Results: A genome-wide association study of hydrocephalus in 13 cases and 69 controls using 29,720 SNPs indicated the involvement of a region on ECA1 (P T corresponding to XP_001491595 p.Gln475* was identical to a B3GALNT2 mutation identified in a human case of muscular dystrophy-dystroglycanopathy with hydrocephalus. All 16 available cases and none of the controls were homozygous for the mutation, and all 17 obligate carriers (= dams of cases) were heterozygous. A random sample of the Friesian horse population (n = 865) was tested for the mutation in a commercial laboratory. One-hundred and forty-seven horses were carrier and 718 horses were homozygous for the normal allele; the estimated allele frequency in the Friesian horse population is 0.085. Conclusions: Hydrocephalus in Friesian horses has an autosomal recessive mode of inheritance. A nonsense mutation XM_001491545 c.1423C>T corresponding to XP_001491595 p.Gln475* in B3GALNT2 (1: 75,859,296-75,909,376) is concordant with hydrocephalus in Friesian horses. Application of a DNA test in the breeding programme will reduce the losses caused by hydrocephalus in the Friesian horse population

Dwarfism with joint laxity in Friesian horses is associated with a splice site mutation in B4GALT7

Background: Inbreeding and population bottlenecks in the ancestry of Friesian horses has led to health issues such as dwarfism. The limbs of dwarfs are short and the ribs are protruding inwards at the costochondral junction, while the head and back appear normal. A striking feature of the condition is the flexor tendon laxity that leads to hyperextension of the fetlock joints. The growth plates of dwarfs display disorganized and thickened chondrocyte columns. The aim of this study was to identify the gene defect that causes the recessively inherited trait in Friesian horses to understand the disease process at the molecular level. Results: We have localized the genetic cause of the dwarfism phenotype by a genome wide approach to a 3 Mb region on the p-arm of equine chromosome 14. The DNA of two dwarfs and one control Friesian horse was sequenced completely and we identified the missense mutation ECA14:g.4535550C> T that cosegregated with the phenotype in all Friesians analyzed. The mutation leads to the amino acid substitution p.(Arg17Lys) of xylosylprotein beta 1,4-galactosyltransferase 7 encoded by B4GALT7. The protein is one of the enzymes that synthesize the tetrasaccharide linker between protein and glycosaminoglycan moieties of proteoglycans of the extracellular matrix. The mutation not only affects a conserved arginine codon but also the last nucleotide of the first exon of the gene and we show that it impedes splicing of the primary transcript in cultured fibroblasts from a heterozygous horse. As a result, the level of B4GALT7 mRNA in fibroblasts from a dwarf is only 2 % compared to normal levels. Mutations in B4GALT7 in humans are associated with Ehlers-Danlos syndrome progeroid type 1 and Larsen of Reunion Island syndrome. Growth retardation and ligamentous laxity are common manifestations of these syndromes. Conclusions: We suggest that the identified mutation of equine B4GALT7 leads to the typical dwarfism phenotype in Friesian horses due to deficient splicing of transcripts of the gene. The mutated gene implicates the extracellular matrix in the regular organization of chrondrocyte columns of the growth plate. Conservation of individual amino acids may not be necessary at the protein level but instead may reflect underlying conservation of nucleotide sequence that are required for efficient splicing

Viola striata Aiton

https://thekeep.eiu.edu/herbarium_specimens_byname/20239/thumbnail.jp

Reconstruction using the lotus petal flap:applications and outcomes

This thesis describes the application and outcomes of reconstruction using the lotus petal flap. The lotus petal flap is a fasciocutaneous flap from the groin or infragluteal fold. It can be applied after resection of tumors in the vulvoperineal area. The first study of 93 patients shows that complications occur in about 70% of the patients. Most complications are minor (e.g. wound dehiscence). The quality of life following vulvar reconstruction using the lotus petal flap shows a decrease in quality of life compared to healthy women. However, the quality of life is comparable to patients with vulvar defects which did not need reconstruction with flaps. Sexual functioning is decreased following reconstruction mainly due to pain, but patients are still satisfied with their sexual functioning. The lotus petal flap is known for reconstructions of vulvar defects. It can also be used for reconstruction in the perineal area. The quality of life following perineal reconstruction using the lotus petal flap is comparable to patients with perineal defects which did not need reconstruction with flaps. Sexual activity was clearly decrease following reconstruction using the lotus petal flap, however patients seemed to be satisfied with their sexual functioning. Patients were moderately satisfied with the esthetic outcome. The esthetic outcome scored by patients, laymen and plastic surgeons was comparable

Viola pubescens var. eriocarpa (Schwein.) N.H.Russell

https://thekeep.eiu.edu/herbarium_specimens_byname/20241/thumbnail.jp

Lysimachia lanceolata Walter

https://thekeep.eiu.edu/herbarium_specimens_byname/14637/thumbnail.jp

Lysimachia lanceolata Walter

https://thekeep.eiu.edu/herbarium_specimens_byname/14637/thumbnail.jp