Loss of F-box only protein 2 (Fbxo2) disrupts levels and localization of select NMDA receptor subunits, and promotes aberrant synaptic connectivity

NMDA receptors (NMDARs) play an essential role in some forms of synaptic plasticity, learning, and memory. Therefore, these receptors are highly regulated with respect to their localization, activation, and abundance both within and on the surface of mammalian neurons. Fundamental questions remain, however, regarding how this complex regulation is achieved. Using cell-based models and F-box Only Protein 2 (Fbxo2) knock-out mice, we found that the ubiquitin ligase substrate adaptor protein Fbxo2, previously reported to facilitate the degradation of the NMDAR subunit GluN1 in vitro, also functions to regulate GluN1 and GluN2A subunit levels in the adult mouse brain. In contrast, GluN2B subunit levels are not affected by the loss of Fbxo2. The loss of Fbxo2 results in greater surface localization of GluN1 and GluN2A, together with increases in the synaptic markers PSD-95 and Vglut1. These synaptic changes do not manifest as neurophysiological differences or alterations in dendritic spine density in Fbxo2 knock-out mice, but result instead in increased axo-dendritic shaft synapses. Together, these findings suggest that Fbxo2 controls the abundance and localization of specific NMDAR subunits in the brain and may influence synapse formation and maintenance

Alteration of Microbial Communities Colonizing Leaf Litter in a Temperate Woodland Stream by Growth of Trees Under Conditions of Elevated Atmospheric CO\u3csub\u3e2\u3c/sub\u3e

Elevated atmospheric CO2 can cause increased carbon fixation and altered foliar chemical composition in a variety of plants, which has the potential to impact forested headwater streams because they are detritus-based ecosystems that rely on leaf litter as their primary source of organic carbon. Fungi and bacteria play key roles in the entry of terrestrial carbon into aquatic food webs, as they decompose leaf litter and serve as a source of nutrition for invertebrate consumers. This study tested the hypothesis that changes in leaf chemistry caused by elevated atmospheric CO2 would result in changes in the size and composition of microbial communities colonizing leaves in a woodland stream. Three tree species, Populus tremuloides, Salix alba, and Acer saccharum, were grown under ambient (360 ppm) or elevated (720 ppm) CO2, and their leaves were incubated in a woodland stream. Elevated-CO2 treatment resulted in significant increases in the phenolic and tannin contents and C/N ratios of leaves. Microbial effects, which occurred only for P. tremuloides leaves, included decreased fungal biomass and decreased bacterial counts. Analysis of fungal and bacterial communities on P. tremuloides leaves via terminal restriction fragment length polymorphism (T-RFLP) and clone library sequencing revealed that fungal community composition was mostly unchanged by the elevated-CO2 treatment, whereas bacterial communities showed a significant shift in composition and a significant increase in diversity. Specific changes in bacterial communities included increased numbers of alphaproteobacterial and cytophaga-flavobacter-bacteroides (CFB) group sequences and decreased numbers of betaproteobacterial and firmicutes sequences, as well as a pronounced decrease in overall Gram-positive bacterial sequences

A Unified Approach to Distance-Two Colouring of Graphs on Surfaces

In this paper we introduce the notion of $\Sigma$-colouring of a graph $G$: For given subsets $\Sigma(v)$ of neighbours of $v$, for every $v\in V(G)$, this is a proper colouring of the vertices of $G$ such that, in addition, vertices that appear together in some $\Sigma(v)$ receive different colours. This concept generalises the notion of colouring the square of graphs and of cyclic colouring of graphs embedded in a surface. We prove a general result for graphs embeddable in a fixed surface, which implies asymptotic versions of Wegner's and Borodin's Conjecture on the planar version of these two colourings. Using a recent approach of Havet et al., we reduce the problem to edge-colouring of multigraphs, and then use Kahn's result that the list chromatic index is close to the fractional chromatic index. Our results are based on a strong structural lemma for graphs embeddable in a fixed surface, which also implies that the size of a clique in the square of a graph of maximum degree $\Delta$ embeddable in some fixed surface is at most $\frac32\,\Delta$ plus a constant.Comment: 36 page

Super-Resolution Imaging with Small Organic Fluorophores

Heilemann M, van de Linde S, Mukherjee A, Sauer M. Super-Resolution Imaging with Small Organic Fluorophores. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. 2009;48(37):6903-6908