The structure of insulin at the air/water interface: monomers or dimers?

The hydrophobic character of the air/water interface affects the oligomeric composition of insulin. By using interface-specific vibrational sum frequency spectroscopy and calculations of insulin monomer and dimer second-order nonlinear susceptibilities chi(2), we show that insulin monomers segregate to the air/water interface

Generation of Lasso Peptide-Based ClpP Binders

The Clp protease system fulfills a plethora of important functions in bacteria. It consists of a tetradecameric ClpP barrel holding the proteolytic centers and two hexameric Clp-ATPase rings, which recognize, unfold, and then feed substrate proteins into the ClpP barrel for proteolytic degradation. Flexible loops carrying conserved tripeptide motifs protrude from the Clp-ATPases and bind into hydrophobic pockets (H-pockets) on ClpP. Here, we set out to engineer microcin J25 (MccJ25), a ribosomally synthesized and post-translationally modified peptide (RiPP) of the lasso peptide subfamily, by introducing the conserved tripeptide motifs into the lasso peptide loop region to mimic the Clp-ATPase loops. We studied the capacity of the resulting lasso peptide variants to bind to ClpP and affect its activity. From the nine variants generated, one in particular (12IGF) was able to activate ClpP from Staphylococcus aureus and Bacillus subtilis. While 12IGF conferred stability to ClpP tetradecamers and stimulated peptide degradation, it did not trigger unregulated protein degradation, in contrast to the H-pocket-binding acyldepsipeptide antibiotics (ADEPs). Interestingly, synergistic interactions between 12IGF and ADEP were observed

Melt Emulsions: Influence of the Cooling Procedure on Crystallization and Recrystallization of Emulsion Droplets and their Influence on Dispersion Viscosity upon Storage

Emulsions with crystalline dispersed phase are widely used formulations in life sciences. Unfortunately, these products often are unstable resulting in changes of their rheological behavior. These changes could be linked to shape transformations of crystallized droplets, depending on the cooling rate in their production and temperature of storage. In a thermo-optical method, shape changes were investigated in a broad range of constant cooling rates or cooling rate ramps close to industrial processes. Cooling at slow to moderate cooling rates (0.1–50 K min$^{-1}$), as found in typical stirred vessel tanks, induced self-shaping of droplets during crystallization, which resulted in platelet-like particles and fibers, deviating greatly from the formerly spherical shape. In contrast, fast cooling as found in heat exchangers resulted in mostly spherical shapes less prone to recrystallization-induced instabilities

NGTS-4b: A sub-Neptune transiting in the desert

We report the discovery of NGTS-4b, a sub-Neptune-sized planet transiting a 13th magnitude K-dwarf in a 1.34 d orbit. NGTS-4b has a mass M = 20.6 ± 3.0 M⊕ and radius R = 3.18 ± 0.26 R⊕, which places it well within the so-called ‘Neptunian Desert’. The mean density of the planet (3.45 ± 0.95 g cm−3) is consistent with a composition of 100  per cent H2O or a rocky core with a volatile envelope. NGTS-4b is likely to suffer significant mass loss due to relatively strong EUV/X-ray irradiation. Its survival in the Neptunian desert may be due to an unusually high-core mass, or it may have avoided the most intense X-ray irradiation by migrating after the initial activity of its host star had subsided. With a transit depth of 0.13 ± 0.02 per cent, NGTS-4b represents the shallowest transiting system ever discovered from the ground, and is the smallest planet discovered in a wide-field ground-based photometric survey

Discovery of Bright Variable X-ray Sources in NGC 1569 with Chandra

From the analysis of a ~100 ks Chandra observation of the dwarf starburst galaxy NGC 1569, we have found that the X-ray point sources, CXOU 043048.1+645050 and CXOU 043048.6+645058, showed significant time variability. During this observation, the X-ray flux of CXOU 043048.1+645050 increased by 10 times in only 2 x 10^4 s. Since the spectrum in its bright phase was fitted with a disk blackbody model with kT_in ~0.43 keV and the bolometric luminosity is L_bol ~10^38 ergs s^-1, this source is an X-ray binary with a stellar mass black-hole. Since the spectrum in its faint phase was also fitted with a disk blackbody model, the time variability can be explained by a change of the accretion rate onto the black hole. The other variable source, CXOU 043048.6+645058, had a flat spectrum with a photon index of ~1.6. This source may be an X-ray binary with an X-ray luminosity of several x 10^37 ergs s^-1. In addition, three other weak sources showed possible time variability. Taking all of the variability into account may suggest an abundant population of compact X-ray sources in NGC 1569.Comment: 15 pages including 4 Postscript figures; accepted for publication in ApJ

Light-Weight Wearable Gyroscopic Actuators Can Modulate Balance Performance and Gait Characteristics:A Proof-of-Concept Study

Falling is a major cause of morbidity, and is often caused by a decrease in postural stability. A key component of postural stability is whole-body centroidal angular momentum, which can be influenced by control moment gyroscopes. In this proof-of-concept study, we explore the influence of our wearable robotic gyroscopic actuator “GyroPack” on the balance performance and gait characteristics of non-impaired individuals (seven female/eight male, 30 ± 7 years, 68.8 ± 8.4 kg). Participants performed a series of balance and walking tasks with and without wearing the GyroPack. The device displayed various control modes, which were hypothesised to positively, negatively, or neutrally impact postural control. When configured as a damper, the GyroPack increased mediolateral standing time and walking distance, on a balance beam, and decreased trunk angular velocity variability, while walking on a treadmill. When configured as a negative damper, both peak trunk angular rate and trunk angular velocity variability increased during treadmill walking. This exploratory study shows that gyroscopic actuators can influence balance and gait kinematics. Our results mirror the findings of our earlier studies; though, with more than 50% mass reduction of the device, practical and clinical applicability now appears within reach.</p

First isolation of a rhabdovirus from perch Perca fluviatilis in Switzerland

Perca fluviatilis is a fish species of increasing interest to the Swiss fish farming industry. In recent years, recirculation systems have been specifically set up to increase production. In one of these farms, abnormal spiral swimming associated with elevated mortalities occurred in repeated batches of imported perch shortly after stocking on several occasions. No bacterial or parasitic etiology was detected, but a virus grown in bluegill fry (BF-2) cells was identified as perch rhabdovirus. Subsequent investigations of other samples suggested a viral tropism for the central nervous system (CNS). Phylogenetic analysis of the partial N and entire G gene sequences positioned this isolate in genogroup C of the species Perch rhabdovirus, with high nucleotide and amino acid (aa) sequence identities with the DK5533 strain isolated in Denmark in 1989. Comparative studies using other closely related isolates allowed the distinction of 2 serological Patterns among perch rhabdoviruses and the identification of a proline substitution by a serine in Position 147 of the glycoprotein potentially involved in antigenic differentiation. Even if perch imported onto the farm tested negative by virus isolation prior to transport, they may have been the origin of this outbreak since CNS tissue was not included in the samples that were analyzed. Another possibility might be a sub-clinical infection with a viral load in resident fish too low to be detected. This study reports the first isolation of a perch rhabdovirus in Switzerland, and emphasizes the necessity of optimizing diagnostic tools that facilitate better control of the risks associated with fish translocation