ANALYSIS OF SCYE BEARING MOTION AS APPLICABLE TO THE DESIGN OF A MORPHING SPACESUIT

This thesis describes research supporting the development of the Morphing Upper Torso spacesuit design, which uses robotic augmentation of a rear-entry pressure suit to adjust torso dimensions. This concept has the potential to provide increased mobility, easier ingress/egress of the suit, and reduced astronaut workload during extravehicular operations. A range of motion study has been conducted in which subjects wore simulated shoulder scye bearings while performing selected tasks, with the intent to measure human motion in relation to scye bearing motion. Results of the study include an investigation of the neutral pose of the scye bearings in Earth gravity, an analysis of the angular range of motion observed for the right scye bearing, and the development of a heuristic model to predict scye bearing position and orientation as a function of known arm pose

Thermal Analysis of Propulsion Components for the Europa Clipper Mission

This presentation describes the thermal analysis and model development that occurred for selected components on the propulsion module subsystem of the Europa Clipper mission, which will fly to Jupiter's icy moon Europa and collect science data from orbit. An overview of a bipropellant system is given, as well as a description of a typical thermal propulsion design. A comparison is also provided, describing the unique Europa Clipper thermal design, which is atypical in many respects. The engine thermal model development is also discussed, including hot-firing tests with nozzle convection correlation, as well as thermal vacuum tests to measure and correlate the emissivity of critical nozzle surfaces. A description of engine firing, as well as valve soak back, is also provided, including temperature maps and results of engine cases. A summary is also provided, of lessons learned regarding thermal propulsion considerations

Thermal and Alignment Analysis of the Instrument-Level ATLAS Thermal Vacuum Test

This paper describes the thermal analysis and test design performed in preparation for the ATLAS thermal vacuum test. NASA's Advanced Topographic Laser Altimeter System (ATLAS) will be flown as the sole instrument aboard the Ice, Cloud, and land Elevation Satellite-2 (ICESat-2). It will be used to take measurements of topography and ice thickness for Arctic and Antarctic regions, providing crucial data used to predict future changes in worldwide sea levels. Due to the precise measurements ATLAS is taking, the laser altimeter has very tight pointing requirements. Therefore, the instrument is very sensitive to temperature-induced thermal distortions. For this reason, it is necessary to perform a Structural, Thermal, Optical Performance (STOP) analysis not only for flight, but also to ensure performance requirements can be operationally met during instrument-level thermal vacuum testing. This paper describes the thermal model created for the chamber setup, which was used to generate inputs for the environmental STOP analysis. This paper also presents the results of the STOP analysis, which indicate that the test predictions adequately replicate the thermal distortions predicted for flight. This is a new application of an existing process, as STOP analyses are generally performed to predict flight behavior only. Another novel aspect of this test is that it presents the opportunity to verify pointing results of a STOP model, which is not generally done. It is possible in this case, however, because the actual pointing will be measured using flight hardware during thermal vacuum testing and can be compared to STOP predictions

Thermal Design, Tvac Testing, and Lessons Learned for Critical GSE of ATLAS and the ICESat-2 Mission

This presentation describes the thermal design of the three main of optical components which comprise the Bench Checkout Equipment (BCE) for the Advanced Topographic Laser Altimeter System (ATLAS) instrument, which is flying on the ICESat-2 mission. Thermal vacuum testing of these components is also described in this presentation, as well as a few lessons learned. These BCE components serve as critical GSE for the mission; their purpose is to verify ATLAS is performing well. It has been said that, in one light, the BCE is the most important part of ATLAS, since, without it, ATLAS cannot be aligned properly or its performance verified before flight. Therefore, careful attention was paid to the BCEs thermal design, development, and component-level Tvac testing prior to its use in instrument-level and spacecraft-level Tvac tests with ATLAS. This presentation describes that thermal design, development, and testing, as well as a few lessons learned

Young adults’ attitudes towards vaping content on Instagram: Qualitative interviews utilizing the associative imagery technique

Backgound: Vaping among young adults (18-24), increased 46% from 2017-2018, resulting in adverse health effects and vulnerability to nicotine dependence. Young adults spend three hours per day using social media, particularly Instagram, which is dominated by pro-vaping messages. Therefore, young adults’ exposure to vaping content can result in positive perceptions of vaping. Aim: Using the associative imagery technique, our goal was to understand the favorability of Instagram posts depicting aspects of vaping and how young adults relate to the images. Method: Semi-structured interviews were conducted with 24 young adults using the analytic induction method. Results: Three main themes emerged: 1) the power of color and visual aesthetics, meaning participants were drawn to colorful imagery; 2) distancing, when participants attempted to separate themselves from vaping culture; and 3) the environment influences perceptions, meaning participants paid attention to popular content, which enhanced its perceived credibility. Discussion: The type of social media platform and users' expectations are just as important as the vaping content. Attitudes of social vapers compared to hardcore vapers may indicate specific aspects of content perceived as appealing. Conclusions: Visually appealing vaping content impacts young adults, but they are hesitant to share content as to be labeled as a “vaper.

Elevated Levels of Arachidonic Acid-Derived Lipids Including Prostaglandins and Endocannabinoids Are Present Throughout ABHD12 Knockout Brains: Novel Insights Into the Neurodegenerative Phenotype

Derived from arachidonic acid (AA), the endogenous cannabinoid (eCB) 2-arachidonoyl glycerol (2-AG) is a substrate for α/β hydrolase domain-12 (ABHD12). Loss-of-function mutations of ABHD12 are associated with the neurodegenerative disorder polyneuropathy, hearing loss, ataxia, retinitis pigmentosa, and cataract (PHARC). ABHD12 knockout (KO) mice show PHARC-like behaviors in older adulthood. Here, we test the hypothesis that ABHD12 deletion age-dependently regulates bioactive lipids in the CNS. Lipidomics analysis of the brainstem, cerebellum, cortex, hippocampus, hypothalamus, midbrain, striatum and thalamus from male young (3–4 months) and older (7 months) adult ABHD12 KO and age-matched wild-type (WT) mice was performed on over 80 lipids via HPLC/MS/MS, including eCBs, lipoamines, 2-acyl glycerols, free fatty acids, and prostaglandins (PGs). Aging and ABHD12 deletion drove widespread changes in the CNS lipidome; however, the effects of ABHD12 deletion were similar between old and young mice, meaning that many alterations in the lipidome precede PHARC-like symptoms. AA-derived lipids were particularly sensitive to ABHD12 deletion. 2-AG increased in the striatum, hippocampus, cerebellum, thalamus, midbrain, and brainstem, whereas the eCB N-arachidonoyl ethanolamine (AEA) increased in all 8 brain regions, along with at least 2-PGs. Aging also had a widespread effect on the lipidome and more age-related changes in bioactive lipids were found in ABHD12 KO mice than WT suggesting that ABHD12 deletion exacerbates the effects of age. The most robust effects of aging (independent of genotype) across the CNS were decreases in N-acyl GABAs and N-acyl glycines. In conclusion, levels of bioactive lipids are dynamic throughout adulthood and deleting ABHD12 disrupts the wider lipidome, modulating multiple AA-derived lipids with potential consequences for neuropathology

Our digital futures : technologies without boundaries BILETA 2013 : conference report

BILETA is the British and Irish Law, Education and Technology Association, which was formed in April 1986. Its aim is to promote the use of technology in legal education in UK and Ireland but as everybody knows the association also brings together scholars from all over the world. To this end BILETA holds an annual conference in April hosted this year by the University of Liverpool from Wednesday 10 to Friday 12 April 2013. This year’s theme was “Our digital Futures: Technologies Without Boundaries”. The conference was dynamically organised by Joseph Savirimuthu (Senior Lecturer at the Liverpool Law School), a specialist in Internet and Information Law, whose thorough preparations preserved BILETA’s expected standards of interactivity. Nearly one hundred academics and PhD students came together to present their papers and to share ideas about technologies and innovation in law (...

Brain Levels of Prostaglandins, Endocannabinoids, and Related Lipids Are Affected by Mating Strategies

Background. Endogenous cannabinoids (eCBs) are involved in the development and regulation of reproductive behaviors. Likewise, prostaglandins (PGs) drive sexual differentiation and initiation of ovulation. Here, we use lipidomics strategies to test the hypotheses that mating immediately activates the biosynthesis and/or metabolism of eCBs and PGs and that specific mating strategies differentially regulate these lipids in the brain. Methods. Lipid extractions and tandem mass spectrometric analysis were performed on brains from proestrous rats that had experienced one of two mating strategies (paced or standard mating) and two nonmated groups (chamber exposed and home cage controls). Levels of PGs (PGE2 and PGF2alpha), eCBs (AEA and 2-AG, N-arachidonoyl glycine), and 4 related lipids (4 N-acylethanolamides) were measured in olfactory bulb, hypothalamus, hippocampus, thalamus, striatum, midbrain, cerebellum, and brainstem. Results. Overall, levels of these lipids were significantly lower among paced compared to standard mated rats with the most dramatic decreases observed in brainstem, hippocampus, midbrain, and striatum. However, chamber exposed rats had significantly higher levels of these lipids compared to home cage controls and paced mated wherein the hippocampus showed the largest increases. Conclusions. These data demonstrate that mating strategies and exposure to mating arenas influence lipid signaling in the brain

N-arachidonoyl glycine, an abundant endogenous lipid, potently drives directed cellular migration through GPR18, the putative abnormal cannabidiol receptor

Background: Microglia provide continuous immune surveillance of the CNS and upon activation rapidly change phenotype to express receptors that respond to chemoattractants during CNS damage or infection. These activated microglia undergo directed migration towards affected tissue. Importantly, the molecular species of chemoattractant encountered determines if microglia respond with pro- or anti-inflammatory behaviour, yet the signaling molecules that trigger migration remain poorly understood. The endogenous cannabinoid system regulates microglial migration via CB2 receptors and an as yet unidentified GPCR termed the 'abnormal cannabidiol' (Abn-CBD) receptor. Abn-CBD is a synthetic isomer of the phytocannabinoid cannabidiol (CBD) and is inactive at CB1 or CB2 receptors, but functions as a selective agonist at this Gi/o-coupled GPCR. N-arachidonoyl glycine (NAGly) is an endogenous metabolite of the endocannabinoid anandamide and acts as an efficacious agonist at GPR18. Here, we investigate the relationship between NAGly, Abn-CBD, the unidentified 'Abn-CBD' receptor, GPR18, and BV-2 microglial migration. Results: Using Boyden chamber migration experiments, yellow tetrazolium (MTT) conversion, In-cell Western, qPCR and immunocytochemistry we show that NAGly, at sub-nanomolar concentrations, and Abn-CBD potently drive cellular migration in both BV-2 microglia and HEK293-GPR18 transfected cells, but neither induce migration in HEKGPR55 or non-transfected HEK293 wildtype cells. Migration effects are blocked or attenuated in both systems by the 'Abn-CBD' receptor antagonist O-1918, and low efficacy agonists N-arachidonoyl-serine and cannabidiol. NAGly promotes proliferation and activation of MAP kinases in BV-2 microglia and HEK293-GPR18 cells at low nanomolar concentrations - cellular responses correlated with microglial migration. Additionally, BV-2 cells show GPR18 immunocytochemical staining and abundant GPR18 mRNA. qPCR demonstrates that primary microglia, likewise, express abundant amounts of GPR18 mRNA. Conclusions: NAGly is the most effective lipid recruiter of BV-2 microglia currently reported and its effects mimic those of Abn-CBD. The data generated from this study supports the hypothesis that GPR18 is the previously unidentified 'Abn-CBD' receptor. The marked potency of NAGly acting on GPR18 to elicit directed migration, proliferation and perhaps other MAPK-dependent phenomena advances our understanding of the lipid-based signaling mechanisms employed by the CNS to actively recruit microglia to sites of interest. It offers a novel research avenue for developing therapeutics to elicit a self-renewing population of neuroregenerative microglia, or alternatively, to prevent the accumulation of misdirected, pro-inflammatory microglia which contribute to and exacerbate neurodegenerative disease