Maximum parsimony based resolution of inter-species phylogenetic relationships in citrus l. (Rutaceae) using its of rDNA

The present study aims to analyse phylogenetic relationships, using internal transcribed spacer sequence data of ribosomal DNA (rDNA), across 24 Citrus species and close relatives by the evaluation of several parameters such as nucleotide substitution (r), nucleotide diversity (π) and the estimated values of transition/transversion bias (R). The observed results indicated the presence of a wide divergence pattern of rDNA in subfamily Aurantioideae. Maximum parsimony (MP) analysis inferred divergence pattern in the Citrus genus. We observed seven strongly supported clades among the subfamily Aurantioideae. We postulate that the present investigation provides a more robust topology of Citrus and its close relatives, which can significantly prove as an additional support to resolve the phylogenetic relationships in Citrus genera. Therefore, sequences of noncoding regions should exhibit more phylogenetically informative sites than the coding regions do, which is in accordance with the present study

Screening of Tpo Gene C.1708c<T And C.1978c<G Mutations In Iraqi Patients with Hypothyroidism and Thyroid Cancer

Thirty seven patients (hypothyroidism, n=20 and thyroid cancer, n=17) and twenty five healthy individuals (control) were used in this study. These patients, who attended the endocrinologist in Nuclear Medicine Hospital and Al Yarmouk Nuclear Medicine Department in Baghdad, were selected during a period from July to October 2009. Modified specific locked nucleic acid (LNA) primers were used for the detection of TPO gene mutations which include c.1708 C> T and c.1978 C>G. Seven mutations were found in TPO gene. Mutations involved transition cytosine by thymine at position 1708 of the exon 10 (c.1708C<T) were found in four thyroid cancer patients, while, transversion of cytosine by guanine at the position 1978 of exon 11 (c.1978C<G) were found in one thyroid cancer and two hypothyroidism patients. It can be concluded that the c.1708C<T and / or c.1978C<G mutant TPO in hypothyroidism and thyroid cancer patients studied are very likely to be properly expressed but lacks enzymatic activity

A family of human ß4-galactosyltransferases : cloning and expression of two novel UDP-galactose : ß-N-acetylglucosamine ß1,4-galactosyltransferases, ß4Gal-T2 and ß4Gal-T3

Contains fulltext : 25518___.PDF (publisher's version ) (Open Access

Digitalized to reach and track: a retrospective comparison between traditional and conditional estimate of vaccination coverage and dropout rates using e-Tracker data below one-year children in Bangladesh during-COVID and pre-COVID periodResearch in context

Summary: Background: With an impressive track record in expanding childhood immunization and an inclination to adopt digitalization in healthcare service delivery, Expanded Program on Immunization (EPI) Bangladesh piloted the e-Tracker intervention in Moulvibazar district and Dhaka South City Corporation (Zone-5) from 2019 till the end of 2021. Methods: We retrieved and analyzed the digitalized e-Tracker data of 114,194 infants born between January 1, 2019 and December 31, 2020, with help from Health Management Information System (HMIS) and UNICEF Bangladesh. Childhood vaccination coverage and dropout rates were determined using a 'Traditional approach' traditionally used by WHO and a 'Conditional technique' with a modified denominator. Using a multiple logistic regression model, we examined the effects of COVID-19, birth-cohorts, mother education, and location on vaccination rates (coverages &amp; dropouts) to aid with informed decision-making by the policymakers. Findings: The conditional estimation method yielded a lower full vaccination coverage during pre-COVID period than the national and global reported coverage derived using the ‘traditional method’ (73.4% vs. 89.0% &amp; 81.0%). As expected, while the coverage has decreased, the dropout rate increased “during-COVID” compared to the “pre-COVID” period. However, dropouts were estimated lower in the ‘conditional method.’ The average age (in months) for getting BCG was higher in Moulvibazar (∼2.5 months) than that in Dhaka (∼1.4 months). All birth-cohorts from ‘the during-COVID period had about 30% lower odds of getting fully vaccinated than those from the ‘pre-COVID’ period. Interpretations: Age-cohort-specific analysis showed a decline in coverage rates before and during COVID, but e-Tracker didn't have enough data to draw additional conclusions. The server only stored the child's gender, the caregiver’s monthly salary, and the mother's education. It didn't track any other factors related to dropout rates. The e-Tracker is an excellent tool for measuring real coverage and should be scaled nationwide. Funding: UNICEF, Bangladesh

Cloning and characterization of a close homologue of human UDP-N-acetyl-alpha-D-galactosamine: Polypeptide N-acetylgalactosaminyltransferase-T3, designated GaINAc-T6. Evidence for genetic but not functional redundancy.

Contains fulltext : 184726.pdf (Publisher’s version ) (Open Access

Cloning of a Human UDP-N-Acetyl-alpha-D-Galactosamine: Polypeptide N-Acetylgalactosaminyltransferase That Complements Other GalNac-Transferases in Complete O-Glycosylation of the MUC1 Tandem Repeat

Contains fulltext : 216411.pdf (Publisher’s version ) (Open Access)10 p