The effects of magnetic field (MFs) on in vitro culture of human umbilical cord derived mesenchymal stem cells (hUC-MSCs)

The potential hazardous effects of magnetic field (MF) exposures on individual have been extensively studied. However, a number of studies have surprisingly observed that response to optimal MF has significantly increased the proliferation rate of stem cells. Accordingly, this current study aims to investigate the potential impact of induced Static Magnetic Field (SMF) on human Umbilical Cord-Derived Mesenchymal Stem Cells (hUC-MSCs) using Samarium Cobalt (SmCos). SmCos was selected for the study due to its known strong permanent moment magnetization, and stable against the influence of demagnetization. The I 0,000 of hUC-MSCs at passage 3, seeding in 60 mm petri dish were grown in constant 'direct exposure' (DE) of 21.6 mT SMF, alongside with 'indirect exposure' (IE) and 'negative control' (NC) groups. The growth kinetic experiments in the current study exhibited exponential growth at day four in the DE group, compared to day six for both IE and NC groups. Population doubling time (PDT) was also performed and the results showed DE group contributed significantly to the change in PDT for a period of passage 3 with p<0.05. Cell cycle analysis after 18 hours revealed that DE group entered to the cell cycle at higher percentage where hUC-MSCs exposed with MF was committed into 55.18 % cells in S phase and 2 l.75 % cells at G2/M phase compared to IE. ([3 1-1]-TdR) revealed that DE group gave the highest proliferation capability at all points of seeding density, when compared to IE and NC groups. In addition, flow cytometer analysis discovered no significant difference in the expression of the surface markers of DE group compared to IE and NC. SMF also was observed to have the potential to induce higher expression of pluripotency­associated markers (OCT4, SOX2, NANOG, and REX}) in DE group than in the IE and NC groups as analyzed via RT-PCR. In conclusion, through MF exposure, small quantity of collected MS Cs could be expanded rapidly in a shott period of time. This finding may suggest MF as a new modality to expand MSC for various therapeutic applications

Fabrication, Structural And Electrical Characteristics Of Zinc Oxide (Zno) Thin Films By Direct Current Sputtering.

Semikonduktor jurang tenaga lebar zink oksida (ZnO) telah muncul sebagai bahan yang berpotensi untuk pelbagai aplikasi termasuklah sebagai peranti opto-elektronik seperti diod pemancar cahaya, diod laser dan pengesan. The wide band gap semiconductor of zinc oxide (ZnO) has emerged as a potential material for the fabrication of a range of devices including opto-electronic devices such as light – emitting diodes (LEDs), laser diodes (LDs) and detectors

Comprehensive industrial training kit: Enhancing the preparation of industrial training report among students / Izzatil Husna Arshad ...[et al.]

Faculty of Information Management prepares its final semester undergraduate students for the industrial training period of five months, involving 480-hour equivalent work placement, paid or unpaid, located in an approved industrial site, working under the supervision of experienced Information Professionals. During the industrial training period, the students are expected to contribute to the activities of the workplace and to meet the same demands of work production and responsibility expected by the organization of its regular employees. After completion of the industrial training, students are required to present and submit a report regarding activities and projects involved. To date, there are no comprehensive guidelines and standards in the Faculty of Information Management, Universiti Teknologi MARA to assist student’s industrial training preparation, presentation and report documentation. Therefore, this study intends to identify the awareness of students towards the existence of industrial training guideline and their expectations. A survey has been conducted on 213 students (131 female, 82 male) from the Bachelor of Information Science (Hons) Information Systems Management (IM245), final year bachelor programme, at the Universiti Teknologi MARA Cawangan Kelantan. The findings show that comprehensive and standardized guideline is needed by the students to guide them in writing a good industrial training report. This study also significantly contributes in producing a comprehensive all-in-one booklet which is known as Industrial Training Kit (IT Kit) that contains all forms and guidelines, including terms and conditions, rules and regulations provided by the Ministry of Higher Education (MOHE) and Faculty of Information Management. This comprehensive training kit could also be used as a guideline to assist various faculties in managing their students’ practical trainin

MALAYSIA-INDONESIA BILATERAL RELATIONS: EMPIRICAL STUDY ON THE STAGE OF KNOWLEDGE OF GENERATION X AND Y INDONESIA AND MALAYSIA

Malaysia and Indonesia are traditionally neighboring countries in ASEAN and sharing a historical background, language, culture, and religion. It is undeniable fact that Malaysia-Indonesia relations are often influenced by current national issues such as water territorial, Indonesian immigrants, national product, and others.  Reviews These issues always sparked conflicts and Provoked various actions from Indonesians and dieting. Normally, Indonesians are more sensitives compared with dieting when faced with this situation as well. This article focuses on knowledge and understanding of X and Y Indonesian generation on Malaysia-Indonesia bilateral relations. This article incorporates the qualitative method with primary data obtained through a questionnaire distributed amongst respondents. Respondents are consisted of UIN's first-degree students and staff s and were selected by purposive sampling. Apart from that, focus group discussion and interview session was held to get a view from respondents. This article found that X and Y Indonesian generations have a good understanding of Malaysia. They are also usually aware of the importance of Malaysia-Indonesia bilateral relations. In addition, they acknowledged Malaysia as an essential country for Indonesia's present interest. Respondents also showed high sensitivity regarding competition between Malaysia and Indonesia. The important finding of this writing is both generations s acknowledge the fact that they are closest neighboring country and a good relationship should be maintained in facing the global challeng

Sex Steroid Receptors in Polycystic Ovary Syndrome and Endometriosis: Insights from Laboratory Studies to Clinical Trials

Polycystic ovary syndrome (PCOS) and endometriosis are reproductive disorders that may cause infertility. The pathology of both diseases has been suggested to be associated with sex steroid hormone receptors, including estrogen receptors (ER), progesterone receptors (PRs) and androgen receptors (ARs). Therefore, with this review, we aim to provide an update on the available knowledge of these receptors and how their interactions contribute to the pathogenesis of PCOS and endometriosis. One of the main PCOS-related medical conditions is abnormal folliculo genesis, which is associated with the downregulation of ER and AR expression in the ovaries. In addition, metabolic disorders in PCOS are caused by dysregulation of sex steroid hormone receptor expression. Furthermore, endometriosis is related to the upregulation of ER and the downregulation of PR expression. These receptors may serve as therapeutic targets for the treatment of PCOS-related disorders and endometriosis, considering their pathophysiological roles. Receptor agonists maybe applied to increase the expression of a specific receptor and treat endometriosis or metabolic disorders. In contrast, receptor antagonist functions to reduce receptor expression and can be used to treat endometriosis and induce ovulation. Understanding PCOS and the pathological roles of endometriosis sex steroid receptors is crucial for developing potential therapeutic strategies to treat infertility in both conditions. Therefore, research should be continued to fill the knowledge gap regarding the subject

Fouth industral revolution in developing countries: a case of Bangladesh

This study particularly focuses on Industry 4.0 in developing countries and, in particular, that of Bangladesh, which has been recognised as one of the fastest growing economies in South Asia. Prior to collecting the required data, a review was carried out on Industry 4.0 and the current prospect of it in the developing countries. The field study consisted of ten semi-structured interviews comprising of ten main questions concerning Industry 4.0 with ten scholars from both the Bangladeshi universities and various worldwide corporate sectors. The interviews lasted approximately thirty minutes and were conducted in English. After a successful analysis of all the interview transcripts, the study finds five main challenges along with some opportunities to apply Industry 4.0 in Bangladesh. The challenges include poor infrastructure, availability of cheaper labour, and expensive installation of technologies, lack of government supports and lack of knowledge. With this being the first research project regarding Industry 4.0 in Bangladesh, the findings of the study generate some recommendations that could be effective for the decision makers both in government and the private sector

Fourth industrial revolution in developing countries: a case on Bangladesh

This study particularly focuses on Industry 4.0 in developing countries and, in particular, that of Bangladesh, which has been recognised as one of the fastest growing economies in South Asia. Prior to collecting the required data, a review was carried out on Industry 4.0 and the current prospect of it in the developing countries. The field study consisted of ten semi-structured interviews comprising of ten main questions concerning Industry 4.0 with ten scholars from both the Bangladeshi universities and various worldwide corporate sectors. The interviews lasted approximately thirty minutes and were conducted in English. After a successful analysis of all the interview transcripts, the study finds five main challenges along with some opportunities to apply Industry 4.0 in Bangladesh. The challenges include poor infrastructure, availability of cheaper labour, and expensive installation of technologies, lack of government supports and lack of knowledge. With this being the first research project regarding Industry 4.0 in Bangladesh, the findings of the study generate some recommendations that could be effective for the decision makers both in government and the private sector

IM-Vative sandbox: fun learning in mastering hypertext markup language (HTML) / Izzatil Husna Arshad ...[et al.]

Hypertext Markup Language (HTML) is one of the most popular languages for website development. Since its inception, many academic institutions have integrated the knowledge of HTML within their syllabus. However, previous research shows that many students are having difficulties to be proficient in HTML language. Several problems are identified; (1) lack of effective online content, (2) lack of supporting tool to code HTML, (3) portability issue of authoring software. Therefore, IM-Vative Sandbox was developed based on PHP Programming Language (PHP), MySQL Database, and jQuery. The sandbox provides real-time generation of HTML source codes; compared to the conventional method of using Notepad, Sublime, or any other authoring tools. In terms of commercialization, the application can be accessed through mobile and desktop and suitable for beginners who want to learn the basics of HTM

Penambahbaikan penyediaan Laporan Latihan Industri melalui Sistem Repositori Laporan dan Kit Latihan Industri (enhancing the preparation of Industrial Training Report through Report Repository System and Industrial Training Kit) / Salliza Md Radzi … [et al.]

Kursus Latihan Industri (IMC690) merupakan kursus wajib yang harus diambil oleh pelajar semester 7 bagi program Pengurusan Sistem Maklumat (IM245). Kursus ini membawa pemberat jam kredit yang tinggi iaitu 12 jam kredit. Terdapat empat (4) bahagian yang dinilai dan penyumbang markah sebanyak 40% adalah pada laporan akhir latihan industri. Masalah berlaku apabila pelajar tidak dapat menyiapkan laporan mengikut tempoh yang telah ditetapkan oleh pihak Fakulti. Natijahnya, laporan lewat dihantar, laporan lewat disemak dan markah laporan rendah telah memberi kesan terhadap pelajar dan pensyarah. Objektif kajian adalah bertujuan untuk mengurangkan peratus kelewatan penghantaran laporan latihan industri. Kajian ini mengenengahkan dua (2) projek inovasi kreatif iaitu pembangunan Sistem Repositori Laporan Latihan Industri (ITR2) dan penghasilan Kit Latihan Industri (IT Kit) sebagai kaedah penyelesaian masalah. Berdasarkan dapatan kajian, peratusan kelewatan penyediaan laporan akhir IMC690 telah berjaya diturunkan daripada 60% kepada 0% dan secara tidak langsung telah mendorong kepada peningkatan peratusan markah laporan akhir melebihi paras 30%. Hasil kedua-dua inovasi ini telah terbukti sebagai sumber rujukan komprehensif yang telah berjaya membantu pelajar dalam menghasilkanlaporan yang berkualiti dan mengikut piawaian

The effects of magnetic field (MFs) on in vitro culture of human umbilical cord derived mesenchymal stem cells (hUC-MSCs)

The potential hazardous effects of magnetic field (MF) exposures on individual have been extensively studied. However, a number of studies have surprisingly observed that response to optimal MF has significantly increased the proliferation rate of stem cells. Accordingly, this current study aims to investigate the potential impact of induced Static Magnetic Field (SMF) on human Umbilical Cord-Derived Mesenchymal Stem Cells (hUC-MSCs) using Samarium Cobalt (SmCo5). SmCo5 was selected for the study due to its known strong permanent moment magnetization, and stable against the influence of demagnetization. The 10,000 of hUC-MSCs at passage 3, seeding in 60 mm petri dish were grown in constant ‘direct exposure’ (DE) of 21.6 mT SMF, alongside with ‘indirect exposure’ (IE) and ‘negative control’ (NC) groups. The growth kinetic experiments in the current study exhibited exponential growth at day four in the DE group, compared to day six for both IE and NC groups. Population doubling time (PDT) was also performed and the results showed DE group contributed significantly to the change in PDT for a period of passage 3 with p<0.05. Cell cycle analysis after 18 hours revealed that DE group entered to the cell cycle at higher percentage where hUC-MSCs exposed with MF was committed into 55.18 % cells in S phase and 21.75 % cells at G2/M phase compared to IE. ([3H]-TdR) revealed that DE group gave the highest proliferation capability at all points of seeding density, when compared to IE and NC groups. In addition, flow cytometer analysis discovered no significant difference in the expression of the surface markers of DE group compared to IE and NC. SMF also was observed to have the potential to induce higher expression of pluripotency-associated markers (OCT4, SOX2, NANOG, and REX1) in DE group than in the IE and NC groups as analyzed via RT-PCR. In conclusion, through MF exposure, small quantity of collected MSCs could be expanded rapidly in a short period of time. This finding may suggest MF as a new modality to expand MSC for various therapeutic applications