DEVELOPMENT OF PAPER-BASED ANALYTICAL DEVICE FOR DETECTING DIAZEPAM IN URINE

Objective: The aim of this study was to develop Paper-based Analytical Devices (PADs) with colorimetric method as a presumptive test for detecting diazepam in urine Methods: Colorimetric method was used as a principle of this study. PADs were fabricated with wax-printing methods. Chosen colorimetric reagent was tested for selectivity with hydromorphone and codeine; and sensitivity by measuring the absorbance with UV-Vis spectrophotometer. PADs were tested for its sensitivity and stability. The intensity of color developed on PADs are measured with ImageJ. The ability of PADs to detect diazepam in urine was simulated with testing spiked urine sample to the PADs Results: Zimmermann gave the most obvious prominent color change from colorless to purple-red color out of the four reagents. PAD is selective to diazepam when tested with hydromorphone and codeine. PAD is sensitive with a cut-off concentration at 100 ppm. PAD can detect diazepam in urine with the highest recovery percent at 92.8%±4,6 Conclusion: It can be concluded that PAD is quite selective and sensitive to detect diazepam in urine and can be done easily and fast for onsite analysi

Perbaikan Manajemen Pakan Silase untuk Keberlanjutan Usaha Peternakan Sapi di Era New Normal pada Kelompok Ternak Bago Mulyo

Feeding independence is one of the target achievements desired by every farmer. One of them is the Bago Mulyo livestock group. The Bago Mulyo livestock group is a livestock group located in Gumukmas District, Jember Regency. Several problems of the Bago Mulyo Livestock Group which reduce production by 5% because the prices of production facilities (seeds, fertilizers, pesticides, and feed) are expensive and the distribution is not smooth; the need for food will increase by 5% due to panic buying and food supplies. The distribution of feed was not optimal, making farmers have to find solutions so that feed ingredients are still available. One solution is to utilize existing feed ingredients and maintain their feed quality until they are available throughout the year, namely by silage method. The implementation of the service consists of several activities, the first was coordination with the head of the Bago Mulyo livestock group, purchased silage materials, simulated modified silo tools, implementation of community service, and surveying the results of community service activities. The results of the community service survey which have shown that 100% of farmers who participate in the service activities understand silage technology with the addition of silo modification from previously only 43% who understand silage technology. The conclusion is an increase in understanding of silage technology which is one of the indicators of support for the service team in carrying out service activities for the Bago Mulyo Livestock Group, so that this technology can be applied properly for the sustainability of the cattle business in the Livestock Group

INTEGRATING LOCAL CULTURE TO TEACH SPEAKING FOR THE SEVENTH GRADE STUDENTS OF MTS JAUHAURUL IHSAN JAMBI

The aim of this research is to find out whether or not the local culture as teaching materials give effect on students’ speaking ability. This research is an experimental research, by using one group pre-test post test design which has a class as an experiment class without a control class. There are nine students as the sample. The instrument used of collecting the data is test. Paired sample t-test is used to analyzed the data. In this research, based on the descriptive analysis, the researcher found that the mean score of post test ,which is 20.78, is higher than the mean score of pre test which is 16.22. Moreover, based on the computation of paired sample t-test, the Sig. (2-tailed) was 0.128. It was higher than 0.05. So, there is no significant different on students’ speaking ability than before, after integrating local cultue as teaching materials

THE EFFECT OF ENGLISH COMIC WEBTOON ON STUDENTS’ READING COMPREHENSION AT MADRASAH TSANAWIYAH NEGERI 2 MUARO JAMBI

Penelitian ini bertujuan untuk mengetahui efek Comic Bahasa Inggris Webtoon pada kemampuan pemahaman membaca siswa pada kelas 8 MTsN 2 Muaro Jambi. Penelitian ini adalah kuantitatif dengan menggunakan disain quaieksperimental, dan subjek penelitian ini adalah 52 siswa. Dari hasil analisis peniltian menginidikasikan bahwa hasil dari rata-rata nilai post-test yaitu (77.33) lebih tinggi dari nilai rata-rata pre-test yaitu (61.13). Artinya Ha bisa diterima, oleh karnanya Komik Webtoon Bahasa Inggris bisa meningkatkan kemampuan membaca siswa setelah diberikan perlakuan. Selanjutnya, peneliti telah manganalisa nilai thitung > ttable (22.826 > 4. 064) dan significant 2 –tailed > 0.05 (0.000>0.05). Jadi, Ha diterima, artinya ada peningkatan yang significant dari hasil belajar membaca siswa yang diajarkan dengan menggunakan Komik Webtoon Bahasa Inggris dibanding siswa yang tidak

Kegiatan Social Empowerment Mahasiswa Beasiswa dengan Pembekalan Mengenai Microsoft Office Untuk Eksplorasi Diri di Panti Asuhan Siti Khadijah Al Kubra

Pada era industri 4.0 semua hal di dunia ini menggunakan alat-alat digital. Microsoft office adalah salah satu alat digital yang dengannya orang tidak perlu menulis menggunakan pulpen maupun pensil untuk membuat sebuah surat atau ringkasan, dan Microsoft office pada era industri 4.0 ini juga digunakan untuk kegiatan belajar oleh siswa maupun mahasiswa. Oleh karena itu, kami selaku Mahasiswa Beasiswa Universitas Al-Azhar Indonesia melakukan program Social Empowerment dengan pembekalan mengenai Microsoft office, tepatnya power point dan excel, yang dilaksanakan di Panti Asuhan Siti Khadijah Al Kubra. Kegiatan ini memiliki berbagai rangkaian acara yang mampu membuat anak-anak di Panti Asuhan memiliki wawasan yang lebih luas mengenai Power Point dan Excel. Selain itu, kegiatan ini juga menumbuhkan jiwa sosial dan kepedulian mahasiswa terhadap lingkungan sekitar. Dengan berbagi ilmu, kami sangat berharap memberikan manfaat kepada anak-anak di Panti Asuhan Siti Khadijah Al Kubra. Berdasarkan kegiatan yang telah dilakukan, dapat disimpulkan bahwa anak-anak di Panti Asuhan Siti Khadijah Al Kubra memiliki antusiasme belajar yang sangat tinggi, tetapi mereka memiliki keterbatasan biaya untuk fasilitas seperti komputer ataupun laptop. Namun, segala keterbatasan tersebut tidak membuat anak-anak menjadi putus semangat.Kata Kunci: Industri 4.0, Microsoft office, Social empowermen

Immobilization of trypsin onto porous methacrylate-based monolith for flow-through protein digestion and its potential application to chiral separation using liquid chromatography

Monolithic columns for analytical applications have attracted the researcher’s attention. In this work, the laboratory-made organic-polymer monolithic column is modified with trypsin and further applied as a nanobiocatalyst microreactor and a stationary phase for separating chiral compounds by liquid chromatography. The monolith was synthesized by in-situ copolymerization of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EDMA) or trimethylolpropane trimethacrylate (TRIM) as a crosslinking agent, with porogen of 1,4-butanediol/propanol/water (4:7:1 v/v) and AIBN as the radical polymerization initiator inside PEEK and silicosteel tubings (1.0 mm i.d × 100 mm) at 60oC for 12 h. A total monomer ratio (%T) and crosslinking agent (%C) of 40:25 and 28:12 were applied to prepare poly-(GMA-co-EDMA) and poly-(GMA-co-TRIM), respectively. The produced monoliths were further modified by introducing trypsin (10 mg/L) through the ring-opening reaction of the epoxide group existing in the monolithic column. The trypsin-immobilized poly-(GMA-co-EDMA) monolithic column was applied as the nanobiocatalyst microreactor for online/flow-through and rapid digestion of β-casein sample into its peptide fragments. The trypsin-immobilized poly-(GMA-co-TRIM) column has potential application to be used as the HPLC stationary phase for the separation of R/S-citronellal enantiomers

Cobalt (II)-mediated molecularly imprinted polymer as a monolithic stationary phase for separation of Racemic citronellal by liquid chromatography

A metal-mediated molecularly imprinted polymer (MMIP) monolithic column was prepared as the stationary phase for high performance liquid chromatography (HPLC) and applied to the enantiomeric separation of rac-citronellal. MMIP column was prepared through in situ copolymerizations with the ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate/[BMIM][BF4] as the primary pore-forming agent and cobalt(II) acetate as the metal pivot. Interactions between polymer components in the synthesized monolith were assessed by FTIR to identify the functional groups. 'e monolith morphology was characterized with SEM, and the template removal was detected by UV Spectrophotometry at 253 nm. In this study, (R)-(+)-citronellal was used as a template, whereas 4-vinylpyridine (4-VP) was employed as the functional monomer with two monomer crosslinkers, trimethylolpropanetrimethacrylate (TRIM), and ethylene glycol dimethacrylate (EDMA). The ternary mixture of porogenic solvent consisted of [BMIM][BF4], dimethylformamide (DMF), and dimethyl sulfoxide (DMSO) with the applied ratio of 1 : 1:1 (v/v) and 10 : 1:5 (v/v) for the preparation of MMIP using TRIM and EDMA crosslinkers, respectively. Co(II) ion was added to the porogenic solvent before being mixed with the functional monomer and the crosslinker mixtures. Separating the rac-citronellal was achieved on the synthesized MMIP, showing better selectivity than the monolithic metal-mediated nonimprinted polymer (MNIP), nonimprinted polymer (NIP), and molecularly imprinted polymer (MIP)