Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species

The study aimed to identify the HWP1 gene in non-Candida albicans Candida species and the differential expression of HWP1 following treatment with Piper betle and Brucea javanica aqueous extracts. All candidal suspensions were standardized to 1 x 10(6) cells/mL. The suspension was incubated overnight at 37 degrees C (C. parapsilosis, 35 degrees C). Candidal cells were treated with each respective extract at 1, 3, and 6 mg/mL for 24 h. The total RNA was extracted and reverse transcription-polymerase chain reaction was carried out with a specific primer of HWP1. HWP1 mRNAs were only detected in C. albicans, C. parapsilosis, and C. tropicalis. Exposing the cells to the aqueous extracts has affected the expression of HWP1 transcripts. C. albicans, C. parapsilosis, and C. tropicalis have demonstrated different intensity of mRNA. Compared to P. betle, B. javanica demonstrated a higher suppression on the transcript levels of HWP1 in all samples. HWP1 was not detected in C. albicans following the treatment of B. javanica at 1 mg/mL. In contrast, C. parapsilosis and C. tropicalis were shown to have HWP1 regulation. However, the expression levels were reduced upon the addition of higher concentration of B. javanica extract. P. betle and B. javanica have potential to be developed as oral health product.Article Link : http://www.hindawi.com/journals/ecam/2013/397268

The effects of continuous lighting (CL) method on the growth development of brassica chinensis for led plant factory in wsn application

This study was performed to investigate the best practise on using LED light for optimum growth of Brassica Chinensis and reduce turn around time at different kind of photoperiod study utilizing Wireless Sensor Network (WSN) technology as remote monitoring system. Growth performance of Brassica Chinensis under two different wavelengths (blue and red) 16: 4 as light source has been used to determine plant growth performance and phytochemicals aspect of plant characteristics. Two experiments were conducted which is the pulse treatment (1 hour light and 1 hour dark) and continuous light (CL) photoperiod treatment in both trials. Observation such as leaves count, height, dry weight and chlorophy I & ll of both plants were analysed. It was noted that the CL photoperiod has significant effect on overall growth performance and remarkably lead to improve the efficiency of the plant factory. In order to reason on data and monitor the environmental parameters of the plant factory, an intelligent system using embedded system has been developed to automate the LED control and manipulation. The result shows that the system is stable and has referential significantly in the area of plant factory or indoor farming system

PEMURNIAN dan KARAKTERISASI BIODIESEL dari MINYAK BIJI KELOR (Moringa oleifera) dengan MENGGUNAKAN ADSORBEN BENTONIT (PURIFICATION and CHARACTERIZATION OF BIODIESEL from KELOR SEED OIL (Moringa oleifera) with ADSORBENT BENTONITE)

Abstrak: Biodiesel dari minyak biji kelor dapat diperoleh melalui reaksi esterifikasi dan transesterifikasi. Reaksi transesterifikasi menghasilkan biodiesel kotor yang mengandung metil ester, gliserol dan pengotor lain berupa sisa reaktan dan katalis yang akan mempengaruhi kualitas dari biodiesel sehingga harus dihilangkan dari produk. Tujuan penelitian ini adalah menyiapkan biodiesel dari minyak biji kelor, dan mempelajari pengaruh metode dry washing menggunakan adsorben bentonit. Metode dry washing diterapkan untuk mendapatkan biodiesel dengan kandungan metil ester dengan kualitas yang baik. Hasil penelitian menunjukkan bahwa, kondisi optimum didapatkan pada waktu interaksi antar biodiesel dengan adsorben selama 3 jam dengan karakteristik flash point 185 oC; pour point -4 oC; indeks setana 54,3 dan viskositas 4,17cSt. Dengan demikian karakteristik biodiesel tersebut telah sesuai dengan standar SNI dan dibandingkan dengan penelitian pemurnian biodiesel menggunakan air hasil karakteristik biodiesel yang diperoleh lebih baik. Untuk selanjutnya perlu dilakukan penelitian dengan waktu interaksi lebih dari 3 jam untuk mendapatkan waktu interaksi optimum.Kata kunci: Biodiesel, Minyak biji kelor, bentonit, Dry washingAbstract: Biodiesel from moringa seed oil can be obtained through esterification and transesterification reactions. Transesterification reaction produces biodiesel gross containing methyl ester, glycerol, and other impurities such as residual reactants and catalysts that will affect the quality of biodiesel and should be removed from the product. The purpose of this study is to prepare biodiesel from kelor seed oil, and studied the effect of dry washing method using bentonite adsorbents. Dry washing method is applied to obtain the content of methyl ester biodiesel with good quality. The results showed that the optimum conditions obtained at the time of the interaction between biodiesel with adsorbent for 3 hours with the characteristics of flash point of 185 ° C; pour point of -4 ° C; cetane index of 54.3 and a viscosity of 4.17 cSt. Thus the characteristics of biodiesel has been in accordance with ISO standards and compared to biodiesel using water purification research results obtained biodiesel characteristics better. To further research needs to be done for theKeywords: Biodiesel, kelor seed oil, bentonite, Dry washing

Mutu Bakso Ikan Ekor Kuning (Caesio Cuning) Dengan Penambahan Rebung (Dendrocalamus Asper)

This study aims to get the exact formulation of the slurry shoots with yellowtail fish meat in the manufacture of composite flour meatballs using sago starch and tapioca starch as a filler. The expected benefits of this research is the improve of the nutritional value of the meatballs with the addition of bamboo shoots and increase the diversity of fishery product processing and dissemination of fish balls. The method used in this research is completely randomized design (CRD) with 5 treatments and 3 replications. The treatment in this study is P1 (25% bamboo shoots : 35% yellowtail fish), P2 (20% bamboo shoots : 40% yellowtail fish), P3 (15% bamboo shoots : 45% yellowtail fish), P4 (10% bamboo shoots : 50% yellowtail fish), P5 (5% bamboo shoots : 55% yellowtail fish). The results of this study showed that the addition ratio of shoots on yellowtail fish balls significant effect on water content, fiber content and protein levels as well as color, aroma, flavor and texture of the meatballs are descriptive, but no effect was not apparent to the hedonic test on the attributes of color, texture and an overall assessment. The best treatment is P1 (25% bamboo shoots : 35% yellowtail fish)

Pemanfaatan Kulit Pisang Kepok (Musa Paradisiacalinn) dalam Pembuatan Dodol

The aim of this study to get the best formulation dodol with Glutenioos Rice Flour and kepok banana peelsin different ratios, as well as to determine the preference level panelists to dodol produced. This study uses a Completely Randomized Design (CRD) with four treatments and four replications: SN1 (Glutenioos Rice Flour 90% : Kepok Banana Peel 10%), SN2 (Glutenioos Rice Flour 85% : Kepok Banana Peel 15%), SN3 (Glutenioos Rice Flour 80% : Kepok Banana Peel 20%) and SN4 (Glutenioos Rice Flour 75% : Kepok Banana Peel 25%). The results showed that the number of comparisons kepok banana peel and glutnioos rice flour significantly affected the moisture content, ash content, fat content, crude fiber content, sucrose concent, assessment of organoleptic descriptive (color, aroma, flavour and texture) and assessment of organoleptic hedonic overall. The best result of treatment formulation was SN4 (Glutenioos Rice Flour 75% : Kepok Banana Peel 25%) with a moisture content of 16,05%, ash content of 0,50%, fat content 7,85%, crude fiber content 3,09% and sucrose content 46,02%, while the organoleptic assessment results descriptive, dodol generated slightly brown colored, somewhat fragrant flavorful typical banana peel, taste sweet, chewy texture and overall liking ratings for dodol produced is like

Passively mode-locked laser using an entirely centred erbium-doped fiber

This paper describes the setup and experimental results for an entirely centred erbium-doped fiber laser with passively mode-locked output. The gain medium of the ring laser cavity configuration comprises a 3 m length of two-core optical fiber, wherein an undoped outer core region of 9.38 μm diameter surrounds a 4.00 μm diameter central core region doped with erbium ions at 400 ppm concentration. The generated stable soliton mode-locking output has a central wavelength of 1533 nm and pulses that yield an average output power of 0.33 mW with a pulse energy of 31.8 pJ. The pulse duration is 0.7 ps and the measured output repetition rate of 10.37 MHz corresponds to a 96.4 ns pulse spacing in the pulse train

Removal of Basic Yellow 51 Dye by Using Ion Exchange Resin Obtained by Modification of Byproduct Sugar Beet Pulp

The removal of dyestuffs, which are an important pollutant group in textile wastewater, is an important environmental problem. Various chemical and physicochemical methods are used in the purification of these wastewaters. Recently, it is known that a wide variety of agricultural materials and their modified products have been investigated for dye removal using the adsorption process, which is a promising removal method. In this study, the removal of Basic Yellow 51 dyestuff from aqueous solutions of sugar beet pulp, first activated by saponification with NaOH solution and then modified by heating with citric acid, was investigated. For this purpose, the effects of parameters such as solution pH, sorbent dose, initial concentration, contact time and temperature on the sorption of Basic Yellow 51 dye to modified sugar beet pulp were investigated. As a result, it was found that the functional groups of pectin and cellulose substances in sugar beet pulp, which are effective in sorption, were activated and their number increased, thereby increasing the dye removal efficiency. It was found that sorption efficiency increased with increasing contact time and temperature and decreased with increasing initial concentration. It was determined that the sorption equilibrium data fit the Langmuir isotherm better and that the sorption process was endothermic and spontaneous. The q(e) value was obtained from the Langmuir isotherm as approximately 200 mg/g. As a final result, it has been demonstrated that the modified product of sugar beet pulp can be used effectively in removing basic dyes from the aquatic environment