PREPARATION OF PLANT MUCILAGE CLARIFICANTS AND THEIR EFFECT ON JAGGERY PROCESSING OF SUGARCANE VARIETY Co 86032

Objective: The aim of the study was to prepare jaggery from sugarcane variety Co86032 using plants mucilage as clarificants and to determine the effect of plant mucilage on jaggery processing.Methods: Production of jaggery using five plant clarificants namely Aloe vera, Flax seeds, Fenugreek, Purslane and Malabar spinach at three different concentrations i.e., 0.1%, 0.2% and 0.4% of raw sugarcane juice and the jaggery prepared without any clarificants serves as control. During production, the processing parameters such as quantity of scum removed, total time taken for processing and jaggery yield was determined.Results: It was found that the jaggery prepared using plant mucilage at concentration of 0.4% found superior when compared to 0.2%, 0.1% and control respectively. Aloe vera at 0.4% removed maximum scum (4.07%), taken minimum processing time (72.33 min) and recorded maximum jaggery yield (10.92 kg). The efficacy of Aloe vera as mucilage clarificants in jaggery production was followed by fenugreek, flax seeds, purslane and Malabar spinach in the order respectively.Conclusion: The application of plants mucilage as clarificants in the jaggery production removed significant level of scum, reduced processing time and improved yield of jaggery. Therefore the selected plant mucilages can be used as clarificants in the jaggery production and can be considered as potential alternative to chemical clarificants.Â

MODELLING AND ANALYSIS OF HEAVY VEHICLE CHASSIS FOR COMPOSITE MATERIALS FOR HEFTY LOAD CIRCUMSTANCES

The composite cloth is a cloth composed of or more excellent stages and having bulk homes considerably great from the ones of any of the components. Different sorts of composite material are to be had and in fact, considered one of its far polymer matrix composites. It can be very well-known due to their low rate and smooth fabrication strategies. It has the advantages of immoderate tensile energy, excessive stiffness, and particular corrosion resistance and so on. A gift, this polymer matrix composite fabric is implemented in aerospace, vehicle industries because of its miles the immoderate energy to low weight ration. For cars, chassis includes an assembly of all the essential factors of a truck to be prepared for operation on the street. In our mission, format, and model the heavy vehicle chassis with the useful aid of the use of seasoned/engineer software program software, through taking the information from the l & t heavy car version by using the use of opposite engineering techniques. Presently used the material for chassis is metallic. The critical motive is to replace the chassis cloth with im 7 fiber & 997 epoxy. By using metallic, the weight of the chassis is more in assessment with im 7 fiber & 997 epoxy, due to the fact its density is greater. Structural and modal assessment is finished on chassis for optimizing the above parameters beneath the 10tons load. And we're using layer stacking approach for 3 and five layers for evaluation of im 7 fiber & 997 epoxy

Exploiting Explainability to Design Adversarial Attacks and Evaluate Attack Resilience in Hate-Speech Detection Models

The advent of social media has given rise to numerous ethical challenges, with hate speech among the most significant concerns. Researchers are attempting to tackle this problem by leveraging hate-speech detection and employing language models to automatically moderate content and promote civil discourse. Unfortunately, recent studies have revealed that hate-speech detection systems can be misled by adversarial attacks, raising concerns about their resilience. While previous research has separately addressed the robustness of these models under adversarial attacks and their interpretability, there has been no comprehensive study exploring their intersection. The novelty of our work lies in combining these two critical aspects, leveraging interpretability to identify potential vulnerabilities and enabling the design of targeted adversarial attacks. We present a comprehensive and comparative analysis of adversarial robustness exhibited by various hate-speech detection models. Our study evaluates the resilience of these models against adversarial attacks using explainability techniques. To gain insights into the models' decision-making processes, we employ the Local Interpretable Model-agnostic Explanations (LIME) framework. Based on the explainability results obtained by LIME, we devise and execute targeted attacks on the text by leveraging the TextAttack tool. Our findings enhance the understanding of the vulnerabilities and strengths exhibited by state-of-the-art hate-speech detection models. This work underscores the importance of incorporating explainability in the development and evaluation of such models to enhance their resilience against adversarial attacks. Ultimately, this work paves the way for creating more robust and reliable hate-speech detection systems, fostering safer online environments and promoting ethical discourse on social media platforms

Effects of the Molecular Weight and the Degree of Deacetylation of Chitosan Oligosaccharides on Antitumor Activity

Effects of the degree of deacetylation (DDA) and the molecular mass of chitosan oligosaccharides (CTS-OS), obtained from the enzymatic hydrolysis of high molecular weight chitosan (HMWC), on antitumor activity was explored. The DDA and molecular weights of CTS-OS were determined by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-TOF MS) analysis. The CTS-OS were found to be a mixture of mainly dimers (18.8%), trimers (24.8%), tetramers (24.9%), pentamers (17.7%), hexamers (7.1%), heptamers (3.3%), and octamers (3.4%). The CTS-OS were further fractionated by gel-filtration chromatography into two major fractions: (1) COS, consisting of glucosamine (GlcN)n, n = 3–5 with DDA 100%; and (2) HOS, consisting of (GlcN)5 as the minimum residues and varying number of N-acetylglucosamine (GlcNAc)n, n = 1–2 with DDA about 87.5% in random order. The cytotoxicities, expressed as the concentration needed for 50% cell death (CC50), of CTS-OS, COS, and HOS against PC3 (prostate cancer cell), A549 (lung cancer cell), and HepG2 (hepatoma cell), were determined to be 25 μg·mL−1, 25 μg·mL−1, and 50 μg·mL−1, respectively. The HMWC was approximately 50% less effective than both CTS-OS and COS. These results demonstrate that the molecular weight and DDA of chitosan oligosaccharides are important factors for suppressing cancer cell growth

Effect of Reinforcement of Oral Health Education Message through Short Messaging Service in Mobile Phones: A Quasi-Experimental Trial

Objective. This paper aims to assess the effectiveness of reinforcement of oral health education message through short messaging service (SMS) in mobile phones. Material and Methods. 400 subjects from two colleges (200 from each college) belonging to 18-20 years age group possessing mobile phones were randomly selected and baseline examination of oral hygiene and gingival status was carried out using Oral Hygiene Index (OHI) and Gingival Index (GI). Oral health education was provided to all the subjects. Oral health education message was reinforced through short messaging service (SMS) in mobile phones for the subjects belonging to the intervention group. There was no such reinforcement for the control group. Follow-up examinations were done at the end of 1st, 2nd, 3rd, and 6th month. After the 3rd month, subjects of the intervention group did not receive oral health education message through short messaging service (SMS) and were followed up after next three months. Compiled data was analyzed using SPSS version 16 statistical software. Result. Mean OHI and GI scores in intervention group were significantly ( < 0.01) less than those of control group after the 2nd, 3rd, and 6th month. Conclusion. Reinforcement of oral health education message through short messaging service (SMS) is effective media to improve oral health

Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells

Cancer gene therapy requires the design of non-viral vectors that carry genetic material and selectively deliver it with minimal toxicity. Non-viral vectors based on cationic natural polymers can form electrostatic complexes with negatively-charged polynucleotides such as microRNAs (miRNAs). Here we investigated the physicochemical/biophysical properties of chitosan–hsa-miRNA-145 (CS–miRNA) nanocomplexes and the biological responses of MCF-7 breast cancer cells cultured in vitro. Self-assembled CS–miRNA nanocomplexes were produced with a range of (+/−) charge ratios (from 0.6 to 8) using chitosans with various degrees of acetylation and molecular weight. The Z-average particle diameter of the complexes was <200 nm. The surface charge increased with increasing amount of chitosan. We observed that chitosan induces the base-stacking of miRNA in a concentration dependent manner. Surface plasmon resonance spectroscopy shows that complexes formed by low degree of acetylation chitosans are highly stable, regardless of the molecular weight. We found no evidence that these complexes were cytotoxic towards MCF-7 cells. Furthermore, CS–miRNA nanocomplexes with degree of acetylation 12% and 29% were biologically active, showing successful downregulation of target mRNA expression in MCF-7 cells. Our data, therefore, shows that CS–miRNA complexes offer a promising non-viral platform for breast cancer gene therapy

Acute and subacute in vivo safety assessment of developed chitosan derivatives for food applications

The acute and subacute studies of two chitosan derivatives was conducted by oral administration on Swiss albino mice. Chitosan soluble derivatives were prepared by depolymerization method using potassium persulfate. 13C NMR data have revealed that Low Molecular Weight Chitosan (LMWC) has higher (∼70%) ‘acetylated’ residues and Self Assembled Chitosan Microparticles (SAMC) consists of ‘deacetylated’ residues. For acute toxicity study, a single dose of two concentrations 2000 and 5000 mg/kg bw of the chitosan derivatives (LMWC, or SAMC) was given orally to healthy mice. Results indicated that the LD50 value for chitosan derivatives was greater than 5000 mg/kg bw. In the sub-acute toxicity studies, administration of concentrations 1000 and 2000 mg/kg bw dose of chitosan derivatives (repeated daily dose for 28 days) also did not reveal any toxicological changes in clinical observations, viz. relative organs, body weight, food consumption, biochemical parameters studied, antioxidant markers, and histopathological studies has been compared with control group. Together, present systematic study supports the oral administration of non-viscous and soluble chitosan derivatives (LMWC &amp; SAMC) will have no toxicity may be safe to use in food and pharma applications

α-Lipoic acid ameliorates altered colonic contractility and intestinal transit in STZ-diabetic rats

279-282α-Lipoic acid treatment (100 mg/kg/day for 2 weeks after 6 weeks of untreated diabetes) of streptozotocin diabetic rats partially but significantly reversed both reduced contractile response of distal colon to acetylcholine and delayed transit of charcoal meal in small intestine compared to diabetic control. These effects of α-Lipoic acid were associated with complete reversal of diabetes induced increased plasma lipid peroxidation level. α-Lipoic acid had no effect on any of the parameters measured in non-diabetic rats. These findings demonstrate contribution of oxidative stress in the development of physiological changes of gut in diabetes