Calcium-dependent binding between calmodulin and lysozyme

AbstractCalmodulin, an acidic protein that binds calcium with high affinity, has multiple roles in the activation of many enzymes involved in cellular regulation of eukaryotes. In this study we show that calmodulin binding to hen egg-white lysozyme, in a Ca2+-dependent way, was observed using electroblots incubated with biotinylated calmodulin and detected with avidin-alkaline phosphatase or for affinity chromatography on a gel calmodulin column. Antimicrobial activity of lysozyme was not modified in the presence of Ca2+-calmodulin

Streptomycetes: a new model to study cell death

Colonies of streptomycetes are now viewed as multicellular entities containing morphologically and biochemically differentiated cell types which have specific functions and precise spatial relationships to one another. Like multicellular organisms, colony development in streptomycetes is also maintained by a tight balance between cell proliferation and cell death processes. This review describes the current state of knowledge concerning cell death in streptomycetes

Predictive value of angiogenesis-related gene profiling in patients with HER2-negative metastatic breast cancer treated with bevacizumab and weekly paclitaxel

Bevacizumab plus weekly paclitaxel improves progression-free survival (PFS) in HER2-negative metastatic breast cancer (mBC), but its use has been questioned due to the absence of a predictive biomarker, lack of benefit in overall survival (OS) and increased toxicity. We examined the baseline tumor angiogenic-related gene expression of 60 patients with mBC with the aim of finding a signature that predicts benefit from this drug. Multivariate analysis by Lasso-penalized Cox regression generated two predictive models: one, named G-model, including 11 genes, and the other one, named GCmodel, including 13 genes plus 5 clinical covariates. Both models identified patients with improved PFS (HR (Hazard Ratio) 2.57 and 4.04, respectively) and OS (HR 3.29 and 3.43, respectively). The G-model distinguished low and high risk patients in the first 6 months, whereas the GC-model maintained significance over timeThis work was supported by a grant from the Independent Clinical Research Program (EC10-342), Independent Clinical Research Program (EC10-342), ISCIII (Instituto de Salud Carlos III), Spanish Ministry of Health, and funded also from Roche Farma S.A.U

Diseño y aplicación de un modelo difuso para el estudio de la estabilidad de taludes en terrenos volcánicos

Máster Univ. en Gestión e Innovac.Tecnológica en la Construcción Por la Ul

La Criatura de Frankenstein y la lucha por el reconocimiento

This text of Mary Shelley permits the possibility of considering the character of the «Creature» from the Axel Honneth`s perspective of the Ethics of reconnaissance. From this point of view, the dramatic sense of this novel moves round identity´s problem. The essential condition to build identity is the recognition from each to other. That is why identity can not be obtain in the sense of solitarious consciousness, but requires of intersubjetivity. The Creature becomes deprived of recognition, even by Frankenstein who has created him. This Creature becomes perverse because this privation, when the most elemental right of human being so as being someone and having an identity, is denied to him. His wickedness is the secondary effect of exclusion and suffering.Este texto de Mary Shelley, permite la posibilidad de considerar el personaje de la «Criatura» desde la perspectiva de la Ética del reconocimiento, de Axel Honneth. Desde este punto de vista, la carga dramática de esta novela gira en torno al problema de la identidad. La condición imprescindible para construir la identidad es el reconocimiento por parte del otro. Por eso la identidad no se puede adquirir en el plano subjetivo de la conciencia solitaria sino que precisa de la intersubjetividad . La criatura se ve privada de reconocimiento, incluso por parte de Frankenstein, que lo ha engendrado. Esa privación, al negarle el derecho más elemental del ser humano, que es ser alguien y tener una identidad, lo vuelve perverso. Su maldad es el efecto secundario de la marginación y el sufrimiento

OSNA Total Tumor Load for the Prediction of Axillary Involvement in Breast Cancer Patients: Should We use Different Thresholds According to the Intrinsic Molecular Subtype? MOTTO Study

Breast cancer; Molecular subtype; Total tumor loadCáncer de mama; Subtipo molecular; Carga tumoral totalCàncer de mama; Subtipus molecular; Càrrega tumoral totalAims: To assess the impact of the molecular subtype (MS) on the total number of CK19 mRNA copies in all positive SLN (TTL) threshold, to predict non-SLN affectation, and to compare 5 years progression-free survival (PFS) according to the risk of recurrence (ROR) group by PAM50. Methods: Cohort with infiltrating breast cancer with intra-operative metastatic SLN detected by one-step nucleic acid amplification (OSNA) assay who underwent subsequent ALND. Logistic regression was used to assess a possible interaction between TTL and MS(Triple Negative, Her-2-Enriched, Luminal A, or Luminal B), or hormone receptors (HR: positive or negative) by immunohistochemistry (IMH). Cox regression was used to compare PFS and OS in the 3 ROR groups (high, medium, or low). Results: TTL was predictive of non-SLN affectation in both univariate (OR [95% CI]: 1.72 [1.43, 2.05], P < .001) and multivariate (1.55 [95% CI: 1.04, 2.32], P = .030) models, but MS-IMH or HR-IMH, and their interactions with TTL were not (best multivariate model: HR + main effect OR 1.16 [95% CI: 0.18, 7.64], P = .874; interaction OR: 1.04 [0.7, 1.55], P = .835; univariate model: HR + main effect OR: 1.44 [95% CI: 0.85, 2.44], P = .180). PFS was lower in the high-risk ROR group (81.1%) than in the low-risk group (93.9%) (HR: 3.68 [95 CI: 1.70, 7.94], P < .001). Conclusions: our results do not provide evidence to support the utilization of subtype-specific thresholds for TTL values to make therapeutic decisions on the axilla. The ROR group was predictive of 5 years-PFS.This study was funded by Sysmex España, S.L