45 research outputs found
Regulation of Axonal HCN1 Trafficking in Perforant Path Involves Expression of Specific TRIP8b Isoforms
The functions of HCN channels in neurons depend critically on their subcellular localization, requiring fine-tuned machinery that regulates subcellular channel trafficking. Here we provide evidence that regulatory mechanisms governing axonal HCN channel trafficking involve association of the channels with specific isoforms of the auxiliary subunit TRIP8b. In the medial perforant path, which normally contains HCN1 channels in axon terminals in immature but not in adult rodents, we found axonal HCN1 significantly increased in adult mice lacking TRIP8b (TRIP8b−/−). Interestingly, adult mice harboring a mutation that results in expression of only the two most abundant TRIP8b isoforms (TRIP8b[1b/2]−/−) exhibited an HCN1 expression pattern similar to wildtype mice, suggesting that presence of one or both of these isoforms (TRIP8b(1a), TRIP8b(1a-4)) prevents HCN1 from being transported to medial perforant path axons in adult mice. Concordantly, expression analyses demonstrated a strong increase of expression of both TRIP8b isoforms in rat entorhinal cortex with age. However, when overexpressed in cultured entorhinal neurons of rats, TRIP8b(1a), but not TRIP8b(1a-4), altered substantially the subcellular distribution of HCN1 by promoting somatodendritic and reducing axonal expression of the channels. Taken together, we conclude that TRIP8b isoforms are important regulators of HCN1 trafficking in entorhinal neurons and that the alternatively-spliced isoform TRIP8b(1a) could be responsible for the age-dependent redistribution of HCN channels out of perforant path axon terminals
A di-arginine ER retention signal regulates trafficking of HCN1 channels from the early secretory pathway to the plasma membrane
Calciumionen-Aktivit�t im L�slichkeitsgleichgewicht zwischen dem Apatit des Zahnschmelzes und dem Speichel
Exploring Belize and its Barrier Reef: Formation, Ecosystem, Culture, and Human Influence on the Reef.
Color poster with text, images, charts, and maps.The Mesoamerican Reef is the largest reef system in the Western Hemisphere. The origin and evolution of the Belize Barrier reef in the
southern portion of this system is strongly controlled by local tectonics along the boundary between the North American Plate and the
Caribbean Plate. This project focused on the modern and ancient geomorphology of the
carbonate dominated region. Students explored the
country's geology, ecology,and culture.University of Wisconsin--Eau Claire Office of Research and Sponsored Programs
Evolution des paysages du Néolithique à nos jours dans la péninsule de La Hague (Normandie, France), l'exemple de l'Anse Saint-Martin
Untersuchungen �ber den Einflu� von Methylenblau und Brillantkresylblau auf das Wachstum des Ehrlich-Ascites-Tumors
Evolution des paysages du Néolithique à nos jours dans la péninsule de La Hague (Normandie, France), l'exemple de l'Anse Saint-Martin
Evolution des paysages et anthropisation depuis le Néolithique dans la péninsule de La Hague (Normandie, France)
International audienc
Synorogenic Basion Evolution in the Cordillera Frontal, Argentina
Color poster with text, maps, and charts describing research conducted by Taylor Crist, Michelle Forgette, Bryan Hardel, Phil Larson, Shane Peterson, Julia Potter, and Heidi Stanek; advised by J. Brian Mahoney.The Argentinian Andes are located in South America between ~ 20-35 S latitude. The focus area of study is the synorogenic basin development associated with the Aconcagua Fold and Thrust Belt (AFTB).University of Wisconsin--Eau Claire Office of Research and Sponsored Programs