Characterization of Avidin and Case9 Single Protein Molecules by a Solid-state Nanopore Device

The shape and charge of a protein play significant roles in protein dynamics in the biological system of humans and animals. Characterizing and quantifying the shape and charge of a protein at the single-molecule level remains a challenge. Solid-state nanopores made of silicon nitride (SiNx) have emerged as novel platforms for biosensing such as diagnostics for single-molecule detection and DNA sequencing. SSN detection is based on measuring the variations in ionic conductance as charged biomolecules translocate through nanometer-sized channels driven by an external voltage applied across the membrane. In this paper, we observe the translocation of asymmetric cylindrical structure CRISPR-Cas9 protein and symmetric cylindrical structure Avidin protein driven by an electric field through the solid-state nanopore. We also observe how glycerol impacts on the time durations and current blockage amplitudes produced by the translocation of two proteins in nanopore by using different glycerol concentrations

A Revisit of Fake News Dataset with Augmented Fact-checking by ChatGPT

The proliferation of fake news has emerged as a critical issue in recent years, requiring significant efforts to detect it. However, the existing fake news detection datasets are sourced from human journalists, which are likely to have inherent bias limitations due to the highly subjective nature of this task. In this paper, we revisit the existing fake news dataset verified by human journalists with augmented fact-checking by large language models (ChatGPT), and we name the augmented fake news dataset ChatGPT-FC. We quantitatively analyze the distinctions and resemblances between human journalists and LLM in assessing news subject credibility, news creator credibility, time-sensitive, and political framing. Our findings highlight LLM's potential to serve as a preliminary screening method, offering a promising avenue to mitigate the inherent biases of human journalists and enhance fake news detection

Aluminum Zintl anion moieties within sodium aluminum clusters

Through a synergetic combination of anion photoelectron spectroscopy and density functional theory based calculations, we have established that aluminum moieties within selected sodium-aluminum clusters are Zintl anions. Sodium–aluminum cluster anions, Na m Al n −, were generated in a pulsed arc discharge source. After mass selection, their photoelectron spectrawere measured by a magnetic bottle, electron energy analyzer. Calculations on a select sub-set of stoichiometries provided geometric structures and full charge analyses for both cluster anions and their neutral cluster counterparts, as well as photodetachment transition energies (stickspectra), and fragment molecular orbital based correlation diagrams

A Logical Pattern Memory Pre-trained Model for Entailment Tree Generation

Generating coherent and credible explanations remains a significant challenge in the field of AI. In recent years, researchers have delved into the utilization of entailment trees to depict explanations, which exhibit a reasoning process of how a hypothesis is deduced from the supporting facts. However, existing models often overlook the importance of generating intermediate conclusions with logical consistency from the given facts, leading to inaccurate conclusions and undermining the overall credibility of entailment trees. To address this limitation, we propose the logical pattern memory pre-trained model (LMPM). LMPM incorporates an external memory structure to learn and store the latent representations of logical patterns, which aids in generating logically consistent conclusions. Furthermore, to mitigate the influence of logically irrelevant domain knowledge in the Wikipedia-based data, we introduce an entity abstraction approach to construct the dataset for pre-training LMPM. The experimental results highlight the effectiveness of our approach in improving the quality of entailment tree generation. By leveraging logical entailment patterns, our model produces more coherent and reasonable conclusions that closely align with the underlying premises. Code and Data are released at https://github.com/YuanLi95/T5-LMPMComment: Accepted By Coling 202

Visual-Text Cross Alignment: Refining the Similarity Score in Vision-Language Models

It has recently been discovered that using a pre-trained vision-language model (VLM), e.g., CLIP, to align a whole query image with several finer text descriptions generated by a large language model can significantly enhance zero-shot performance. However, in this paper, we empirically find that the finer descriptions tend to align more effectively with local areas of the query image rather than the whole image, and then we theoretically validate this finding. Thus, we present a method called weighted visual-text cross alignment (WCA). This method begins with a localized visual prompting technique, designed to identify local visual areas within the query image. The local visual areas are then cross-aligned with the finer descriptions by creating a similarity matrix using the pre-trained VLM. To determine how well a query image aligns with each category, we develop a score function based on the weighted similarities in this matrix. Extensive experiments demonstrate that our method significantly improves zero-shot performance across various datasets, achieving results that are even comparable to few-shot learning methods.Comment: 22 pages, 16 figures, published to ICML 202

Application of bioabsorbable screw fixation for anterior cervical decompression and bone grafting

OBJECTIVES: To examine the application of bioabsorbable screws for anterior cervical decompression and bone grafting fixation and to study their clinical effects in the treatment of cervical spondylosis. METHODS: From March 2007 to September 2012, 56 patients, 36 males and 20 females (38-79 years old, average 58.3±9.47 years), underwent a novel operation. Grafts were fixed by bioabsorbable screws (PLLA, 2.7 mm in diameter) after anterior decompression. The bioabsorbable screws were inserted from the midline of the graft bone to the bone surface of the upper and lower vertebrae at 45 degree angles. Patients were evaluated post-operatively to observe the improvement of symptoms and evaluate the fusion of the bone. The Japanese Orthopaedic Association (JOA) score was used to evaluate the recovery of neurological functions. RESULTS: All screws were successfully inserted, with no broken screws. The rate of symptom improvement was 87.5%. All of the grafts fused well with no extrusion. The average time for graft fusion was 3.8±0.55 months (range 3-5 months). Three-dimensional reconstruction of CT scans demonstrated that the grafts fused with adjacent vertebrae well and that the screws were absorbed as predicted. The MRI findings showed that the cerebrospinal fluid was unobstructed. No obvious complications appeared in any of the follow-up evaluations. CONCLUSIONS: Cervical spondylosis with one- or two-level involvement can be effectively treated by anterior decompression and bone grafting with bioabsorbable screw fixation. This operative method is safe and can avoid the complications induced by metal implants

The Evaluation of the Clinical, Laboratory, and Radiological Findings of 16 Cases of Brucellar Spondylitis

Objective. To evaluate the clinical, laboratory, and radiological presentation of 16 cases of brucellar spondylitis.Methods. The clinical manifestations, laboratory tests, and imaging findings of 16 patients (aged from 24 to 66 years) with brucellar spondylitis treated between September 2012 and September 2014 at the Second Affiliated Hospital of Xi’an Jiaotong University (Xi’an, China) were retrospectively analyzed.Results. Clinical manifestations included high fever, severe pain, sweating, and fatigue. One patient had epididymitis, and two showed clear signs of spinal nerve damage. Laboratory tests showed elevated erythrocyte sedimentation rate (ESR) and C-reactive protein content. Serum brucella agglutination tests were positive, and 11 brucella blood cultures were positive. Imaging manifestations mainly consisted of abnormal signals in the intervertebral space or abnormal signals in the adjacent vertebral bodies (16/16, 100%) in magnetic resonance imaging (MRI), disc space narrowing (14/16, 88%) in X-ray and MRI, or bone destruction and sclerosis around the damaged zone (13/16, 81%) in computed tomography, with rare cases of psoas abscess (2/16, 13%) and sequestrum (1/16, 6%).Conclusion. Since brucellar spondylitis exhibited characteristic clinical and imaging manifestations, it could be diagnosed with specific laboratory tests. Early MRI examination of suspected cases could improve rapid diagnosis.</jats:p

Medium access control for inter-gateway handoff support in multi-hop wireless mesh networks

Wireless mesh networks (WMNs) have emerged to be a key wireless technology to support large-scale wireless Internet access. Seamless inter-gateway handoff support is an essential issue to ensure continuous communications in multi-hop WMNs. When the movement of a mobile mesh node (MN) causes its attachment point change in the Internet, the complete handoff process may include two steps: the link-layer handoff and the network-layer handoff. During the network-layer handoff, network- layer signaling packets need to be transmitted between the MN and the Internet via the multi-hop wireless mesh backbone. Due to the multi-hop transmission of network- layer handoff signaling packets, the handoff performance in WMNs can be largely degraded by the long queueing delay and medium access delay at each mesh router, especially when the backbone traffic volume is high. However, this critical issue is ignored in existing handoff solutions of multi-hop WMNs. In addition, the channel contention between data packets and handoff signaling packets is not considered in existing medium access control (MAC) designs. In this research, the seamless handoff support is addressed from a different perspec- tive. By eliminating channel contentions between data and handoff signaling pack- ets, the queueing delay and channel access delay of signaling packets are reduced, while data throughput is maintained. Since various WMNs have different channel resources and hardware cost requirements, four MAC schemes are proposed to im- prove the multi-hop handoff performance in single-channel single-radio, single-channel multi-radio, multi-channel single-radio, and multi-channel multi-radio WMNs. With the proposed MAC schemes, the inter-gateway handoff performance can be improved significantly in multi-hop WMNs