For Better or Worse: Theorizing Adolescent Media Consumption

Few scholars have gone to the lengths of Turkle and boyd to examine the current digital moment under the lens of socialization. Even more crucial is their consideration of youth within the technological context. While adults are more readily adaptive and have autonomy over their media use as they are able to select technological use and frequency, the same cannot be said of younger generations. When considering youth who are primarily between the ages of 9 and 18, it is evident that they have grown alongside digital developments which defines their childhood by its forceful presence through the adults around them. As they grow, youth come to understand the world from different perspectives based by their participation in society, and seek communities similar to their interests. The way that adolescents define community in culture has a radically different face from what previous social media-less generations, but what is at the core of socialization? Is youth’s understanding and ability to create a community overtly changed through digitalization, or is it simply altered? This paper examines how young people grow to respond to technology through a comparative analysis of the works of Sherry Turkle and danah boyd who contrast in analytical perspectives but maintain the same argument that technology is impacting our lives, especially when considering those who are growing up alongside it. Under these circumstances, it is pivotal to recognize the importance of research to understand exactly how such groups are altered through technological dependence in development. The scope will examine how technology is presented to children, how they proceed to utilize it within their youth, and considering the perspective that thinkers such as Turkle and boyd would take in regards to the topic

Effect of plyometric training on swimming block start performance in adolescents

This study aimed to identify the effect of plyometric training (PT), when added to habitual training (HT) regimes, on swim start performance. After the completion of a baseline competitive swim start, 22 adolescent swimmers were randomly assigned to either a PT (n = 11, age: 13.1 ± 1.4 yr, mass: 50.6 ± 12.3 kg, stature: 162.9 ± 11.9 cm) or an HT group (n = 11, age: 12.6 ± 1.9 yr, mass: 43.3 ± 11.6 kg, stature: 157.6 ± 11.9 cm). Over an 8-week preseason period, the HT group continued with their normal training program, whereas the PT group added 2 additional 1-hour plyometric-specific sessions, incorporating prescribed exercises relating to the swimming block start (SBS). After completion of the training intervention, post-training swim start performance was reassessed. For both baseline and post-trials, swim performance was recorded using videography (50Hz Canon MVX460) in the sagital plane of motion. Through the use of Silicon Coach Pro analysis package, data revealed significantly greater change between baseline and post-trials for PT when compared with the HT group for swim performance time to 5.5 m (−0.59 s vs. −0.21 s; p < 0.01) and velocity of take-off to contact (0.19 ms−1 vs. −0.07 ms−1; p < 0.01). Considering the practical importance of a successful swim start to overall performance outcome, the current study has found that inclusion of suitable and safely implemented PT to adolescent performers, in addition to HT routines, can have a positive impact on swim start performance

#5 - Identifying cellular mechano-biological responses to PEG-based hydrogels

Cells sense and respond to mechanical stimuli from their external environment through a process called mechanotransduction. Focal adhesions are integrin-containing, multiprotein structures through which mechanical force is transmitted between the extracellular matrix and the interacting cell. Cells convert the transmitted force into biological responses including migration, proliferation and differentiation. The Garcia lab has previously engineered an integrin-specific hydrogel system resulting in changes in mesenchymal stem cell (MSC) gene expression, secretome, and ultimately regenerative capacity in a murine bone repair model. However, the mechano-biological mechanism driving this cell response to varying hydrogel biophysical and biochemical properties has yet to be studied. Here we have developed relationships between various hydrogel properties and cellular responses (cell adhesion, YAP localization, cell area, and cell shape). We engineered PEG-based hydrogel systems with two different polymerization chemistries, malemide and norbornene, to explore the effect of hydrogel chemistry on MSC cell adhesion and spreading. Using rheology, we demonstrated that hydrogel mechanical properties can be tuned by altering the weight percent of PEG macromer, while adhesion ligand type and density had no effect on hydrogel mechanical properties. PEG-4MAL gels were used for the remaining studies because that chemistry resulted in improved spreading and cell adhesion over norbornene hydrogels. By varying the density of RGD, the adhesive ligand for αvβ3 integrin, on the hydrogels, we showed that higher RGD densities resulted in greater YAP nuclear localization. We sought to understand the mechano-biological signaling pathway involved in YAP nuclear localization by inhibiting ROCK and FAK, proteins critical in mechanosensing via focal adhesion complexes. The inhibition of ROCK, and FAK decreased cell spread area, increased cell circularity and decreased YAP nuclear localization. Taken together this data demonstrates that external signals from PEG-based hydrogels as well as the intracellular signaling cascades involving ROCK and FAK can modulate YAP mechanosensing in MSCs

Two-dimensional kinematics and dynamical modelling of the ‘Jackpot’ gravitational lens from deep MUSE observations

We present results from the first spatially resolved kinematic and dynamical modelling analysis of the unique SDSSJ0946+1006 (‘Jackpot’) triple-source lens system, where a single massive foreground z = 0.222 galaxy multiple-images three background sources at different redshifts. Deep integral field unit spectroscopic data were obtained using the MUSE instrument on the VLT, which, compared to previous single-slit observations, provides full azimuthal area coverage, high sensitivity (5 h integration) and high angular resolution (0.5 arcsec full width at half-maximum). To account for the strong continuum contributions from the z = 0.609 source, a multiple-component stellar template fitting technique is adopted to fit to the spectra of both the lens galaxy and the bright lensed background arc simultaneously. Through this, we robustly measure the first and second moments of the 2D stellar kinematics out to about 10 kpc from the centre of the lens, as well as resolving the inner profile inwards to ∼1 kpc. The 2D kinematic maps show a steep velocity dispersion gradient and a clear rotational component. We constrain the characteristic properties of the stellar and dark matter (DM) mass components with a sufficiently flexible parametrised dynamical model and an imposed lensing mass and find a DM density slope of γ = 1.73+0.17 −0.26, i.e. significantly steeper than an unmodified NFW profile (γ = 1) and consistent with a contracted DM halo. Our fitted models have a lensing-equivalent density slope of η = 0.96 ± 0.02, and thus we confirm most pure lensing results in finding a near isothermal profile for this galaxy

Contrasting roles for DNA methyltransferases and histone deacetylases in single-item and associative recognition memory

Recognition memory enables us to judge whether we have encountered a stimulus before and to recall associated information, including where the stimulus was encountered. The perirhinal cortex (PRh) is required for judgment of stimulus familiarity, while hippocampus (HPC) and medial prefrontal cortex (mPFC) are additionally involved when spatial information associated with a stimulus needs to be remembered. While gene expression is known to be essential for the consolidation of long-term recognition memory, the underlying regulatory mechanisms are not fully understood. Here we investigated the roles of two epigenetic mechanisms, DNA methylation and histone deacetylation, in recognition memory. Infusion of DNA methyltransferase inhibitors into PRh impaired performance in novel object recognition and object-in-place tasks while infusions into HPC or mPFC impaired object-in-place performance only. In contrast, inhibition of histone deacetylases in PRh, but not mPFC, enhanced recognition memory. These results support the emerging role of epigenetic processes in learning and memory

The influence of facility and home pen design on the welfare of the laboratory-housed dog

We have an ethical and scientific obligation to Refine all aspects of the life of the laboratory-housed dog. Across industry there are many differences amongst facilities, home pen design and husbandry, as well as differences in features of the dogs such as strain, sex and scientific protocols. Understanding how these influence welfare, and hence scientific output is therefore critical. A significant proportion of dogs&rsquo; lives are spent in the home pen and as such, the design can have a considerable impact on welfare. Although best practice guidelines exist, there is a paucity of empirical evidence to support the recommended Refinements and uptake varies across industry. In this study, we examine the effect of modern and traditional home pen design, overall facility design, husbandry, history of regulated procedures, strain and sex on welfare-indicating behaviours and mechanical pressure threshold. Six groups of dogs from two facilities (total n=46) were observed in the home pen and tested for mechanical pressure threshold. Dogs which were housed in a purpose-built modern facility or in a modern design home pen showed the fewest behavioural indicators of negative welfare (such as alert or pacing behaviours) and more indicators of positive welfare (such as resting) compared to those in a traditional home pen design or traditional facility. Welfare indicating behaviours did not vary consistently with strain, but male dogs showed more negative welfare indicating behaviours and had greater variation in these behaviours than females. Our findings showed more positive welfare indicating behaviours in dogs with higher mechanical pressure thresholds. We conclude that factors relating to the design of home pens and implementation of Refinements at the facility level have a significant positive impact on the welfare of laboratory-housed dogs, with a potential concomitant impact on scientific endpoints

Domestically Acquired NDM-1–Producing Pseudomonas aeruginosa, Southern California, USA, 2023 - Volume 29, Number 11—November 2023 - Emerging Infectious Diseases journal - CDC

We describe a case of New Delhi metallo-β-lactamase 1-producing carbapenem-resistant Pseudomonas aeruginosa (CRPA) in a transplant patient with multiple hospitalizations in California, USA. Whole-genome sequencing revealed the isolate was genetically distinctive, despite ≈95% similarity to other global strains. The patient's lack of international travel suggests this CRPA was acquired domestically

Multiwavelength radio observations of the compact starburst in Arp 220

We report the first detection at multiple radio wavelengths (13, 6, and 3.6 cm) of 18 compact sources within both nuclei of the Ultra Luminous Infra-Red Galaxy (ULIRG) Arp 220. In just over half of the sources we find that the observed spectra are consistent with the standard model of powerful Type IIn supernovae interacting with their pre-explosion stellar wind. The rate of appearance of new radio sources ascribed to these supernova events suggests that a large fraction of core-collapse supernovae in Arp 220 are highly luminous, possibly implying a radically different stellar initial mass function (IMF) or stellar evolution compared to galactic disks. A second group of sources, consisting of the brightest and longest monitored sources at 18 cm, do not easily fit the radio supernova model. We propose that these are young supernova remnants that have just begun interacting with their surrounding dense ISM