The Stellar Populations of the Carina Dwarf Spheroidal Galaxy: I. a New Color-Magnitude Diagram for the Giant and Horizontal Branches

We report on the first in a series of studies of the Carina dwarf spheroidal galaxy, a nearby satellite of our Galaxy. Our two major results are: 1) precise BI photometry (\sigma_{B-I} \simlt 0.05 for V \simlt 22) for 11,489 stars in the Carina field, and 2) the detection of two, morphologically distinct, horizontal branches, which confirms that star formation in Carina occurred in two well-separated episodes. The old horizontal branch and RR Lyrae instability strip belong to a > 10 Gyr stellar population, while the populous red-clump horizontal branch belongs to an approximately 6 Gyr stellar population. We derive a distance modulus $(m-M)_0=20.09 \pm 0.06$ for Carina from the apparent magnitudes of the old horizontal branch and the tip of the red giant branch, and discuss modifications to the previously estimated distance, total magnitude, and stellar ages. Using the color of the red giant branch, we estimate the metallicities of the younger and older populations to be [Fe/H] = -2.0 and -2.2, respectively.Comment: 18 pages, 9 figures, uses AAS LaTex macros, PostScript figures available through anonymous ftp, accepted for publication in the Astronomical Journal, DAO-tsh94-

Characteristics of pine needle combustion in a semi-gasifier burner

Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, June 2011."June 2011." Cataloged from PDF version of thesis.Includes bibliographical references (p. 29).The motivation behind this report was to develop a stronger understanding of pine needle combustion behavior in a semi-gasifier, in order to ultimately design an effective pine needle cook stove for people in the developing world. Pine needles are a cheap, energy-dense fuel source that can be harnessed for cooking purposes, but they are often physically or chemically altered prior to being used as a fuel. The challenge remains to develop a stove that uses unprocessed pine needles. Using principles of a semi-gasification biomass stove, this paper explores the combustion and burn characteristics of unprocessed pine needles by measuring the effects of temperature in a configured burner when altering primary and secondary airflow into the burner. Experiments showed that pine needles in a semi-gasifier combust in two regimes--one characterized by secondary combustion of volatiles released from the primary burn of raw pine needles, and another characterized by the smoldering of pine needle charcoal. Each regime needed unique balances between primary air and secondary airflow. The first regime required a relatively low primary airflow with a high secondary airflow, while the second regime needed a high primary airflow to maintain charring and low or no secondary airflow. The balance of airflow in the first regime was crucial in determining whether the volatiles combusted or were released as plumes of smoke. The findings of this report can be used as an initial benchmark and resource in the further development of an existing proof-of-concept prototype, which was built by the authors with a team of MIT students and affiliates and a community partner in Uttarakhand, India.by Liane Jessica Fang and Caroline M. Hane-Weijman.S.B

Dwarf Cepheids in the Carina Dwarf Spheroidal Galaxy

We have discovered 20 dwarf Cepheids (DC) in the Carina dSph galaxy from the analysis of individual CCD images obtained for a deep photometric study of the system. These short-period pulsating variable stars are by far the most distant (~100 kpc) and faintest (V ~ 23.0) DCs known. The Carina DCs obey a well-defined period-luminosity relation, allowing us to readily distinguish between overtone and fundamental pulsators in nearly every case. Unlike RR Lyr stars, the pulsation mode turns out to be uncorrelated with light-curve shape, nor do the overtone pulsators tend towards shorter periods compared to the fundamental pulsators. Using the period-luminosity (PL) relations from Nemec et al. (1994 AJ, 108, 222) and McNamara (1995, AJ, 109, 1751), we derive (m-M)_0 = 20.06 +/- 0.12, for E(B-V) = 0.025 and [Fe/H] = -2.0, in good agreement with recent, independent estimates of the distance/reddening of Carina. The error reflects the uncertainties in the DC distance scale, and in the metallicity and reddening of Carina. The frequency of DCs among upper main sequence stars in Carina is approximately 3%. The ratio of dwarf Cepheids to RR Lyr stars in Carina is 0.13 +/- 0.10, though this result is highly sensitive to the star-formation history of Carina and the evolution of the Horizontal Branch. We discuss how DCs may be useful to search effectively for substructure in the Galactic halo out to Galactocentric distances of ~100 kpc.Comment: 20 pages of text, 7 figure

Utilizing Gene Tree Variation to Identify Candidate Effector Genes in Zymoseptoria tritici

Zymoseptoria tritici is a host-specific, necrotrophic pathogen of wheat. Infection by Z. tritici is characterized by its extended latent period, which typically lasts two weeks, and is followed by extensive host cell death and rapid proliferation of fungal biomass. This work characterizes the level of genomic variation in 13 isolates for which we have measured virulence on 11 wheat cultivars with differential resistance genes. Between the reference isolate, IPO323, and the 13 Australian isolates we identified over 800,000 single nucleotide polymorphisms, of which ~10% had an effect on the coding regions of the genome. Furthermore we identified over 1700 probable presence/absence polymorphisms in genes across the Australian isolates using de novo assembly. Finally, we developed a gene tree sorting method that quickly identifies groups of isolates within a single gene alignment whose sequence haplotypes correspond with virulence scores on a single wheat cultivar. Using this method we have identified <100 candidate effector genes whose gene sequence correlates with virulence towards a wheat cultivar carrying a major resistance gene

The western Mediterranean region provided the founder population of domesticated narrow-leafed lupin

Key message: This study revealed that the western Mediterranean provided the founder population for domesticated narrow-leafed lupin and that genetic diversity decreased significantly during narrow-leafed lupin domestication. Abstract: The evolutionary history of plants during domestication profoundly shaped the genome structure and genetic diversity of today’s crops. Advances in next-generation sequencing technologies allow unprecedented opportunities to understand genome evolution in minor crops, which constitute the majority of plant domestications. A diverse set of 231 wild and domesticated narrow-leafed lupin (Lupinus angustifolius L.) accessions were subjected to genotyping-by-sequencing using diversity arrays technology. Phylogenetic, genome-wide divergence and linkage disequilibrium analyses were applied to identify the founder population of domesticated narrow-leafed lupin and the genome-wide effect of domestication on its genome. We found wild western Mediterranean population as the founder of domesticated narrow-leafed lupin. Domestication was associated with an almost threefold reduction in genome diversity in domesticated accessions compared to their wild relatives. Selective sweep analysis identified no significant footprints of selection around domestication loci. A genome-wide association study identified single nucleotide polymorphism markers associated with pod dehiscence. This new understanding of the genomic consequences of narrow-leafed lupin domestication along with molecular marker tools developed here will assist plant breeders more effectively access wild genetic diversity for crop improvement

Stellar population synthesis of post-AGB stars: the s-process in MACHO47.2496.8

The low-metallicity RV Tauri star MACHO47.2496.8, recently discovered in the Large Magellanic Cloud, is highly enriched in carbon and heavy elements produced by the slow neutron capture process (s-process), and is most probably a genuine post-C(N-type) asymptotic giant branch (AGB) star. We use the analysis of the abundances of MACHO47.2496.8 to constrain free parameters in AGB models. We test which values of the free parameters describing uncertain physical mechanisms in AGB stars, namely the third dredge-up and the features of the 13C neutron source, produce models that better match the abundances observed in MACHO47.2496.8. We carry out stellar population synthesis coupled with s-process nucleosynthesis using a synthetic stellar evolution code. The s-process ratios observed in MACHO47.2496.8 can be matched by the same models that explain the s-process ratios of Galactic AGB and post-AGB stars of metallicity > Z_sun/10, except for the choice of the effectiveness of 13C as a neutron source, which has to be lower by roughly a factor of 3 to 6. The less effective neutron source for lower metallicities is also required when comparing population synthesis results to observations of Galactic halo $s$-enhanced stars, such as Pb stars. The 12C/13C ratio in MACHO47.2496.8 cannot be matched simultaneously and requires the occurrence of extra-mixing processes. The confirmed trend of the decreased efficiency of the 13C neutron source with metallicity requires an explanation from AGB s-process models. The present work is to date the first comparison between theoretical models and the detailed abundances of an extragalactic post-AGB star.Comment: accepted for publication on Astronomy & Astrophysics Letter

Influence des niveaux d'alimentation sur les performances de croissance et la rétention azotée chez le poulet de chair

Soixante poulets de chair âgés de 21 jours ont été utilisés dans cette étude, selon quatre niveaux d'alimentation (50g, 75g, 100g et 125 g d'aliment par poulet et par jour). Lors de la mise en lots, les poulets ont été identifiés et pesés individuellement. Au cours de l'étude, les poulets ont été pesés chaque semaine et les collectes des déjections ont été réalisées toutes les deux semaines. Les gains moyens quotidiens ont été influencés significativement (P<O,OOl) aussi bien par l'âge des poulets que par les niveaux d'alimentation. Aussi, l'interaction entre l'âge des poulets et les niveaux d'alimentation" a été significative sur les GMQ (P<0,001). L'âge des poulets et les niveaux d'alimentation n'ont aucune influence significative sur les indices de consommation. Les niveaux d'alimentation ont influencé significativement le rendement carcasse et le gras abdominal (P<0,05). L'âge des poulets a exercé une influence significative sur la rétention d'azote (P<0,00l), ce qui n'est pas les cas des niveaux d'alimentation.Feeding levels influence on growth performance and N-retention in broilersSixt Y 21 day old broiler chickens were used in this studywith four feeding levels (50, 75 100 and 125g offeedlbroiler. day). At the beginning ofthe study the broiler chikens were identified and weighed separately. During the study, the broiler chikens were weighed every week, the foeces were collected every two weeks. The average daily gain (ADG) was significantly influenced bythe levels offeeding and the age ofbroiler chikens (P<O,OOl). The interaction between the age ofbroiler chickens and the levels offeeding had significant influence(p< 0,001) on the ADG.But the age of broiler chikens and the levels of feeding had no carcass significant effect on the feed efficiency. The different levels of feeding have significantly influenced the carcass yield and the abdominal fat (P<O,05). The retention ofnitrogen was significantly influenced by the age ofbroiler chickens (P< 0,001)

Missense-depleted regions in population exomes implicate ras superfamily nucleotide-binding protein alteration in patients with brain malformation.

Genomic sequence interpretation can miss clinically relevant missense variants for several reasons. Rare missense variants are numerous in the exome and difficult to prioritise. Affected genes may also not have existing disease association. To improve variant prioritisation, we leverage population exome data to identify intragenic missense-depleted regions (MDRs) genome-wide that may be important in disease. We then use missense depletion analyses to help prioritise undiagnosed disease exome variants. We demonstrate application of this strategy to identify a novel gene association for human brain malformation. We identified de novo missense variants that affect the GDP/GTP-binding site of ARF1 in three unrelated patients. Corresponding functional analysis suggests ARF1 GDP/GTP-activation is affected by the specific missense mutations associated with heterotopia. These findings expand the genetic pathway underpinning neurologic disease that classically includes FLNA. ARF1 along with ARFGEF2 add further evidence implicating ARF/GEFs in the brain. Using functional ontology, top MDR-containing genes were highly enriched for nucleotide-binding function, suggesting these may be candidates for human disease. Routine consideration of MDR in the interpretation of exome data for rare diseases may help identify strong genetic factors for many severe conditions, infertility/reduction in reproductive capability, and embryonic conditions contributing to preterm loss