The effectiveness of electro-acupuncture in ovarian ischemia reperfusion injury

Electro-acupuncture is widely used in gynecology. Adnexal torsion is an important threat to ovarian reserves in women of reproductive age. This is the first study to investigate whether electro-acupuncture is beneficial in protecting ovarian reserves in case of adnexal torsion resulting in reperfusion injury. Thirty-two female Wistar Albino rats were randomized into four groups: the first group-sham operation, the second group-torsion/detorsion model, the third group-pre-acupunture + torsion/detorsion + post-acupuncture, and the fourth group-torsion/detorsion + post-acupuncture. The acupoints used were CV4 and bilateral SP6, Ex-CA1, Kid3, and ST36. In the third group, the acupoints were needled for two weeks before torsion, continuing for a further two weeks after torsion. In the fourth group, needling began after torsion and was maintained for two weeks. Both histological and biochemical parameters indicating ovarian reserves showed that electro-acupuncture applied to the above points exhibited an ameliorating effect on ovaries injured during ischemia/reperfusion. Electro-acupuncture may be capable of protecting against and preventing ischemia/reperfusion injury in case of ovarian torsion

Examination of the Islamic calligraphy of Yâkut-ül Musta'simî's Mushaf's stored in Turkey museums

Yüksek Lisans Teziİnsanlık tarihi nasıl yazıyla başladı ise İslâm Tarihinin aynı şekilde yazıyla başladığını söylemek yerinde olur. Her türlü fiil davranış ve sözde zarafet, olgunluk, ve hakkaniyete uygunluk gözeten İslâm aynı gayeleri medeniyete dönüşmesinin temel unsurlarından biri olan yazıda da amaçlamıştır. Yazıda daha güzeli aramaya bir silsile şeklinde aktarılarak ulaşılmaya çalışıldığı gibi bazen tarih sürencinde gerçekleştirilen ani dönüşüm ve sıçramalar bu amaca daha fazla hizmet ederek daha çok yol almayı sağlamıştır. Tezimiz İslâm Yazı Sanatı'nda bir dönüşüm veya sıçrama gerçekleştirdiğine inandığımız Yâkût El-Musta'sımî'nin Mushafları üzerinedir. Türkiye müzelerinde bulunan Mushaflarının Hat Sanatı açısından incelemesi yapılmıştır. Yapılan incelemelerde eserlerin orijinalliği ile dönemi öncesi ve sonrasına bakılarak gelişme sürecine verdiği katkılar belirtilmiştir. Harf biçimi, yazı çeşidi, kalem hususiyetleri, süsleme ve kullanılan malzemeler bu kıyaslamada bize yardımcı unsurlar olarak ele alınmıştır.It would be appropriate to say that Islamic History began with writing in the same way as the history of humanity began. Islam, that takes into consideration grace, maturity and fairness in all kinds of deeds, behaviours and expressions, is aimed the same purposes on the Islamic Calligraphy which is one of the basic elements of civilization conversion. As it is tried to be reached a search for finding more beautiful in thecalligraphy by transferring as a series form, sometimes the sudden transformations and jumps in the history process have served this purpose more and provided more progress. Our thesis is on the Mushafs of Yakut Al-Musta'sımî, who has made a transformation or leap in Islamic Calligraphy as believed. TheMushafs, which are stored in the Turkish Museums, are investigated from the perspective of calligraphy.In the investigations, the originality of the Works and their contributions to the development process are indicated by comparing before and after the period. Letters form, type of calligraphy, reedpen characteristics, ornamentation and used materials are handled as an auxiliary elements to this comparison

Regulation of Protein Escape Outside of Vasculars on Ileum and Lung Tissues with Cannabinoids 2 Receptor Agonist (Am1241) In Ischemia/Reperfusion Rat Intestine Model

WOS: 000518456300013Amaç: Kanabinoid 2 reseptör agonistinin İskemi/Reperfüzyon (İ/R) hasarı modelinde antiinflamatuvar etkisinin olup olmadığını kanıtlamaktır. Gereç ve Yöntem: Araştırmada; sıçanlarda barsak iskemi ve reperfüzyon modeli oluşturuldu. Kanabinoid 2 reseptör agonisti (AM-1241), iskemi ve reperfüzyon oluşturmadan hemen önce abdominal venden (iv) verildi. Sonrasında evans mavisi iv olarak uygulandı. Dokulara evans mavisinin geçişi çıplak gözle görüldü. Bu aşamadan hemen sonra sıçanın göğüs kafesi açıldı ve sistemik kan dolaşım havuzu usulüne uygun olarak boşaltıldı. Dokular tartıldıktan sonra 48 saat formamidde inkübasyona bırakıldı ve spektrofotometrede 620 nm dalgaboyunda ölçüm yapıldı. Bulgular: İ/R grubu şam kontrol grubunu göre yaklaşık % 803 evans mavisi kaçışı izlendi. İ/R ve İ/R+CB2 agonist arasındaki fark ise agonistin proteinleri tutup, protein ve evans mavisinin doku sıvısına geçişini azalttığı görülür. Sonuç: Kanabinoid 2 reseptör agonistinin, hem ileum dokusunda ve hem de uzak organda (akciğer) kılcal damarlardan dokuya protein kaçışını engellediği ve dolayısıyla ileum İ/R hasarında antiinflamatuar etki gösterdiği bulundu.Objective: To prove whether the cannabinoid 2 receptor agonist has an anti-inflammatory effect in the model of Ischemia/Reperfusion (I/R) injury. Methods: Intestinal ischemia and reperfusion model was created in rats. The cannabinoid 2 receptor agonist (AM-1241) was given through the abdominal vein (iv) just before creating ischemia and reperfusion. Afterwards, evans blue was applied iv. The transition of evans blue to the tissues was seen with the naked eye. Immediately after this stage, the systemic blood circulation pool was emptied properly. Tissues were incubated at formamide for 48 hours after weighing, and measurements were made at a wavelength of 620 nm on a spectrophotometer. Results: About 803% evans blue escape was observed in the I/R group compared to the sham control group. The difference between the I/R and the I/R+CB2 agonist is that the agonist holds proteins and reduces the transition of protein and evans blue to tissue fluid. Conclusions: The cannabinoid 2 receptor agonist was found to inhibit the escape of protein from both the ileum and the distant organ (lung) capillaries, and thus showed antiinflammatory effect in the ileum I/R injury

Disruption of the ovarian follicle reservoir of prepubertal rats following prenatal exposure to a continuous 900-MHz electromagnetic field

WOS: 000376660100005PubMed: 27007703The effects on human health of electromagnetic field (EMF) have begun to be seriously questioned with the entry into daily life of devices establishing EMF, such as cell phones, wireless fidelity, and masts. Recent studies have reported that exposure to EMF, particularly during pregnancy, affects the developing embryo/fetus. The aim of this study was therefore to examine the effects of exposure to continuous 900-Megahertz (MHz) EMF applied in the prenatal period on ovarian follicle development and oocyte differentiation. Six pregnant Sprague Dawley rats were divided equally into a non-exposed control group (CNGr) and a group (EMFGr) exposed to continuous 900-MHz EMF for 1 h daily, at the same time every day, on days 13-21 of pregnancy. New groups were established from pups obtained from both groups after birth. One group consisting of female pups from CNGr rats was adopted as newborn CNGr (New-CNGr, n = 6), and another group consisting of female pups from EMFGr rats was adopted as newborn EMFGr (New-EMFGr, n = 6). No procedure was performed on New-CNGr or New-EMFGr rats. All rat pups were sacrificed on the postnatal 34th day, and their ovarian tissues were removed. Follicle count, histological injury scoring and morphological assessment with apoptotic index criteria were performed with sections obtained following routine histological tissue preparation. Follicle count results revealed a statistically significant decrease in primordial and tertiary follicle numbers in New-EMFGr compared to New-CNGr (p<0.05), while atretic follicle numbers and apoptotic index levels increased significantly (p<0.05). Histopathological examination revealed severe follicle degeneration, vasocongestion, a low level of increased stromal fibrotic tissue and cytoplasmic vacuolization in granulosa cell in New-EMFGr. Prenatal exposure to continuous 900-MHz EMF for 1 h each day from days 13-21 led to a decrease in ovarian follicle reservoirs in female rat pups at the beginning of the prepubertal period

The effect of prenatal exposure to 900-MHz electromagnetic field on the 21-old-day rat testicle

Demir, Selim/0000-0002-1863-6280WOS: 000327569100022PubMed: 24095929The aim of this study was to investigate the effect of exposure to a 900-MHz electromagnetic field (EMF) in the prenatal term on the 21-old-day rat testicle. Pregnant rats were divided into control (CG) and EMF (EMFG) groups. EMFG was exposed to 900-MHz EMF during days 13-21 of pregnancy. Newborn CG rats were obtained from the CG and newborn EMFG (NEMFG) rats from the EMFG. Testicles were extracted at postnatal day 21. Lipid peroxidation and DNA oxidation levels, apoptotic index and histopathological damage scores were compared. NEMFG rats exhibited irregularities in seminiferous tubule basal membrane and epithelium, immature germ cells in the lumen, and a decreased diameter in seminiferous tubules and thickness of epithelium. Apoptotic index, lipid peroxidation and DNA oxidation were higher in NEMFG rats than in NCG. 21-day-old rat testicles exposed to 900-MHz EMF in the prenatal term may be adversely affected, and this effect persists after birth. Published by Elsevier Inc

Pathological effects of prenatal exposure to a 900 MHz electromagnetic field on the 21-day-old male rat kidney

Mercantepe, Tolga/0000-0002-8506-1755; Erol, Huseyin/0000-0002-9121-536X; Erol, Huseyin Serkan/0000-0002-9121-536XWOS: 000352109500002PubMed: 25158858We investigated the effects on kidney tissue of 900 megahertz (MHz) EMF applied during the prenatal period. Pregnant rats were exposed to 900 MHz EMF, 1 h/day, on days 13-21 of pregnancy; no procedure was performed on control group pregnant rats or on mothers or newborns after birth. On postnatal day 21, kidney tissues of male rat pups from both groups were examined by light and electron microscopy. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione levels also were investigated. Light microscopy revealed some degenerative changes in the tubule epithelium, small cystic formations in the primitive tubules and large cysts in the cortico-medullary or medullary regions in the experimental group. Electron microscopy revealed a loss of peritubular capillaries and atypical parietal layer epithelial cells in the experimental group. Biochemical analysis showed significantly increased MDA levels in the experimental group and decreased SOD and CAT levels. EMF applied during the prenatal period can caused pathological changes in kidney tissue in 21-day-old male rats owing to oxidative stress and decreased antioxidant enzyme levels

Effects of prenatal exposure to a 900 MHz electromagnetic field on 60-day-old rat testis and epididymal sperm quality

WOS: 000377636600002PubMed: 26472053We investigated the effects of exposure in utero to a 900 megahertz (MHz) electromagnetic field (EMF) on 60-day-old rat testis and epididymis. Pregnant rats were divided into control (CG; no treatment) and EMF (EMFG) groups. The EMFG was exposed to 900 MHz EMF for 1 h each day during days 13 - 21 of pregnancy. Newborn rats were either newborn CG (NCG) or newborn EMF groups (NEMFG). On postnatal day 60, a testis and epididymis were removed from each animal. Epididymal semen quality, and lipid and DNA oxidation levels, apoptotic index and histopathological damage to the testis were compared. We found a higher apoptotic index, greater DNA oxidation levels and lower sperm motility and vitality in the NEMFG compared to controls. Immature germ cells in the seminiferous tubule lumen, and altered seminiferous tubule epithelium and seminiferous tubule structure also were observed in hematoxylin and eosin stained sections of NEMFG testis. Nuclear changes that indicated apoptosis were identified in TUNEL stained sections and large numbers of apoptotic cells were observed in most of the seminiferous tubule epithelium in the NEMFG. Sixty-day-old rat testes exposed to 900 MHz EMF exhibited altered sperm quality and biochemical characteristics

Maternal exposure to a continuous 900-MHz electromagnetic field provokes neuronal loss and pathological changes in cerebellum of 32-day-old female rat offspring

WOS: 000381842500010PubMed: 26391347Large numbers of people are unknowingly exposed to electromagnetic fields (EMF) from wireless devices. Evidence exists for altered cerebellar development in association with prenatal exposure to EMF. However, insufficient information is still available regarding the effects of exposure to 900 megahertz (MHz) EMF during the prenatal period on subsequent postnatal cerebellar development. This study was planned to investigate the 32-day-old female rat pup cerebellum following exposure to 900 MHz EMF during the prenatal period using stereological and histopathological evaluation methods. Pregnant rats were divided into control, sham and EMF groups. Pregnant EMF group (PEMFG) rats were exposed to 900 MHz EMF for 1 h inside an EMF cage during days 13-21 of pregnancy. Pregnant sham group (PSG) rats were also placed inside the EMF cage during days 13-21 of pregnancy for 1 h, but were not exposed to any EMF. No procedure was performed on the pregnant control group (PCG) rats. Newborn control group (CG) rats were obtained from the PCG mothers, newborn sham group (SG) rats from the PSG and newborn EMF group (EMFG) rats from the PEMFG rats. The cerebellums of the newborn female rats were extracted on postnatal day 32. The number of Purkinje cells was estimated stereologically, and histopathological evaluations were also performed on cerebellar sections. Total Purkinje cell numbers calculated using stereological analysis were significantly lower in EMFG compared to CG (p < 0.05) and SG (p < 0.05). Additionally, some pathological changes such as pyknotic neurons with dark cytoplasm were observed in EMFG sections under light microscopy. In conclusion, our study results show that prenatal exposure to EMF affects the development of Purkinje cells in the female rat cerebellum and that the consequences of this pathological effect persist after the postnatal period. (C) 2015 Elsevier B.V. All rights reserved

The effects of prenatal exposure to a 900-MHz electromagnetic field on the 21-day-old male rat heart

Mercantepe, Tolga/0000-0002-8506-1755;WOS: 000364542800018PubMed: 25166431The growing spread of mobile phone use is raising concerns about the effect on human health of the electromagnetic field (EMF) these devices emit. the purpose of this study was to investigate the effects on rat pup heart tissue of prenatal exposure to a 900 megahertz (MHz) EMF. For this purpose, pregnant rats were divided into experimental and control groups. Experimental group rats were exposed to a 900MHz EMF (1h/d) on days 13-21 of pregnancy. Measurements were performed with rats inside the exposure box in order to determine the distribution of EMF intensity. Our measurements showed that pregnant experimental group rats were exposed to a mean electrical field intensity of 13.77V/m inside the box (0.50W/m(2)). This study continued with male rat pups obtained from both groups. Pups were sacrificed on postnatal day 21, and the heart tissues were extracted. Malondialdehyde, superoxide dismutase and catalase values were significantly higher in the experimental group rats, while glutathione values were lower. Light microscopy revealed irregularities in heart muscle fibers and apoptotic changes in the experimental group. Electron microscopy revealed crista loss and swelling in the mitochondria, degeneration in myofibrils and structural impairments in Z bands. Our study results suggest that exposure to EMF in the prenatal period causes oxidative stress and histopathological changes in male rat pup heart tissue

Effects of 900-MHz electromagnetic fields exposure throughout middle/late adolescence on the kidney morphology and biochemistry of the female rat

Demir, Selim/0000-0002-1863-6280WOS: 000446534300004PubMed: 30009691The effect of the electromagnetic field (EMF) established when cell phones are in use on human health, and particularly the head, has been the subject of major scientific research. Phones are usually carried near the lumbar region when not in use, and the kidneys will also inevitably be affected by such fields. We investigated the effects on the kidneys of female rats exposed to a continuous 900-megahertz (MHz) EMF for 1 h daily in mid-late adolescence. Control, sham, and EMF groups were established. The EMF was applied to the application group rats daily on postnatal days 35-59. A pseudo-megahertz effect was applied to sham group rats. All animals were euthanized on postnatal day 60. Right kidney tissues were subjected to routine procedures. Malondialdehyde, total antioxidant status, and total oxidant status (TOS) were investigated in left kidneys, and the oxidative stress index (OSI) was also calculated from these. Histopathological analysis revealed no pathology in either the control or sham groups. However, findings including hemorrhage in glomerulus, vacuolization and irregularity in the proximal and distal tubular epithelium, diffuse glomerular degeneration and edema, occasional degeneration in Bowman capsules, hemorrhage in the medullary region, disturbed nucleus location and morphology, and tubular edema in the cortex were observed in the EMF groups. TOS and OSI values were lower in the EMF group (9.4316 +/- 1.0211 and 0.8461 +/- 0.0826, respectively) and the sham group (8.2171 +/- 0.6437 and 0.7358 +/- 0.0545, respectively) than in the control group (11.1522 +/- 1.3389 and 1.0085 +/- 0.1174, respectively) (p < 0.05). In conclusion, exposure to a continuous 900-MHz EMF for 1 h daily during middle and late adolescence may cause various changes in the female rat kidney at postnatal day 60