Isolasi Antimikroba Baru dari Bakteri Tanah

Antimicrobial resistance has become a problem and a threat to health both in Indonesia and in the world. Resistance is the ability of bacteria to neutralize and weaken the action of antibiotics. The aim of this study was to isolate a novel antimicrobial from soil bacteria. The method used in this research is exploratory. The test bacteria used were Staphylococcus aureus and Pseudomonas aeruginosa. The results showed that there were two isolates that had zones of inhibition against the test bacteria staphylococcus aureus, namely isolates T2.2 and T2.18. The results of the first antimicrobial test for isolate T2.2 showed that the inhibition zone formed was 14.05 mm and isolates T2.18 the inhibition zone formed was 11.96 mm. The results of the second antimicrobial test were carried out by treating the unheated T2.2 isolate which showed an inhibition zone formed of 15.53 mm and the heated one 15.46 mm, while the unheated T2.18 isolate showed an average inhibition zone of 12.46 mm and heated 12.21 mm. The conclusion of this study is that the inhibition zone formed on isolates T2.2 and T2.18 is still relatively small and both isolates are spesies of Pseudomonas aeruginosa

Antibiotic Susceptibility Pattern in Clinical Isolates of Pseudomonas aeruginosa

Introduction: Pseudomonas aeruginosa is an opportunistic pathogen and causes of nosocomial infections in hospitals. Due to high antibiotic resistance and the ability to develop new resistance during antibiotic treatment, Pseudomonas aeruginosa infection is difficult to eradicate because the physical treatment becomes difficult and ineffective. This study was conducted to evaluate the antibiotic sensitivity pattern of Pseudomonas aeruginosa strains at two different hospital in Makassar. Methods: This study is a cross-sectional study from March to May 2021. The research samples were taken from the results of culture and antibiotic sensitivity tests conducted at two different hospital, Hasanuddin University Hospital and Dr. Wahidin Sudirohusodo Hospital for the period January 1 to September 30, 2019. A total of 84 samples were cultured and tested for antibiotic sensitivity of Pseudomonas aeruginosa. Results: Antibiotic sensitivity of Pseudomonas aeruginosa was best with aminoglycoside antibiotics, gentamicin (100%) at Hasanuddin University Hospital and amikacin (95.8%) at Dr. Wahidin Sudirohusodo Hospital. At Hasanuddin University Hospital followed by antibiotics amikacin (92.3%) and meropenem (84.6%). At Dr. Wahidin Sudirohusodo Hospital, Pseudomonas aeruginosa also showed good sensitivity to gentamicin (91.5%) and meropenem (77.5%). The sensitivity of Pseudomonas aeruginosa was lowest to piperacillin/tazobactam. Conclusions:  This study shows that the level of effectiveness of the antibiotics meropenem, amikacin and gentamicin is high enough and it can be used as a treatment option in Pseudomonas aeruginosa infection. This study can help as a reference to prevent mortality and morbidity associated with Pseudomonas aeruginosa infection

Study Analisis : Tantangan Orang Tua Dalam Mendidik Anak Dan Mengatasi Kemalasan Belajar Anak Di Era Smart Society 5.0

Zaman terus bergerak maju, Teknologi juga berkembang dari waktu ke waktu. Perkembangan teknologi dapat menimbulkan dampak positif dan negatif. Saat ini, penggunaan perangkat digital sudah mulai memasuki dunia anak-anak. Anak-anak yang hidup di era milenial tidak bisa jauh dari teknologi digital. Penggunaan perangkat pada usia dini telah lama menjadi kontroversi karena alat digital seperti ponsel, computer dan laptop membuat ketagihan sangat tinggi untuk anak-anak. Hal ini dapat mempengaruhi pertumbuhan dan perkembangan karakter anak. Sekarang orang tua menghadapi masalah dalam mendidik anak- anak mereka di era perkembangan digital. Orang tua khawatir tentang perkembangan anak- anak selama periode ini. Sikap orang tua dapat mempengaruhi sikap dan prilaku anak, apalagi pada usia muda.  Sasaran penelitian adalah permasalahan dan tantangan orang tua dalam mendidik atau membesarkan anak di era digital. Penelitian tersebut menggunakan penelitian dengan cara menganalisis dan mengumpulkan data perpustakaan. Data di kumpulkan dan di hubungkan untuk memperoleh jawaban atas permasalahan tersebut. Hasil dari penelitian ini menunjukkan bahwasannya penting sekali bagi orang tua untuk mengetahui tantangan, masalah dan juga solusi yang dapat di temukan saat mendidik anak di era digital sehingga orang tua bisa memantau penggunaan digital pada anak-anak mereka

Molecular Detection of VEB and OXA-23 Resistance Gene in Pseudomonas Aeruginosa Isolates at Dr. Wahidin Sudirohusodo Hospital Makassar

Pseudomonas aeruginosa is a common gram-negative pathogen in nosocomial infections in immune-compromised patients. It exhibits high rates of intrinsic resistance to many classes of antibiotics, especially beta-lactam antibiotics. Production of extended-spectrum beta-lactamase (ESBL) and genes belonging to the carbapenem-hydrolyzing class D subgroup β-lactamases (CHDL) are a problem for increasing antibiotic resistance worldwide. This study aimed to identify P. aeruginosa containing the VEB and OXA-23 genes. Eighty-five clinical isolates of P. aeruginosa from various clinical samples were identified and tested for antimicrobial susceptibility using VITEK 2 compact. VEB and OXA-23 genes were detected using the Polymerase Chain Reaction (PCR) method. The PCR results revealed that 13 (15.3%) of P. aeruginosa isolates were positive OXA-23 gene, but no isolate positive for the VEB gene in P. aeruginosa isolates. The study results demonstrated the spread of the OXA-23 gene in P. aeruginosa isolates at Dr. Wahidin Sudirohusodo Hospital Makassar

Analisis Residu Amoksisilin Pada Hepar dan Ventrikulus Ayam Petelur di Pasar Tradisional Makassar

Residu antibiotik dalam pangan asal hewan terjadi karena tidak memperhatikan waktu henti obat, melebihi dosis yang dianjurkan dan digunakan sebagai feed additive dalam pakan. Residu antibiotik dapat mengancam kesehatan manusia seperti alergi, keracunan, resistensi bakteri dan gangguan jumlah mikroflora dalam saluran pencernaan. Penelitian ini bertujuan untuk mengetahui adanya residu antibiotik golongan amoksisilin pada ayam petelur di pasar tradisional Kota Makassar. Metode yang digunakan adalah pengambilan 24 sampel hepar dan 24 ventriculus ayam petelur pada 4 pasar tradisional di Kota Makassar. Pengukuran kadar residu antibiotik pada sampel yang positif dengan uji sensitivitas antibiotik dilakukan menggunakan HPLC (High Performance Liquid Chromatography). Hasil pengukuran kadar residu antibiotik amoksisilin pada sampel hepar diperoleh konsentrasi 0.0005-0.0010 mg/kg dan pada sampel ventrikulus konsentrasi 0.0002-0.0020 mg/kg. Kadar residu antibiotik amoksisilin pada sampel hati dan ventriculus dibawah nilai Batas Maksimum Residu (BMR) yang ditetapkan SNI 01-636-2000 yaitu 0.01 mg/kg. Hasil penelitian menunjukkan bahwa residu antibiotik amoksisilin yang terdapat pada hepar dan ventrikulus ayam petelur pada 4 pasar tradisional di Kota Makassar berada dibawah nilai BMR

Faktor penentu pembayaran zakat harta di Majlis Agama Islam Negeri Johor

The zakat issue pertaining to the payment and collection of zakat in Malaysia are publicly discussed and critized especially issues related to the payment of zakat to religious authority, Majlis Agama Islam Negeri. In Johor, current scenario shows that there have been an increase in collection and payment of zakat. However according to 2015 statistics, the percentage of zakat payment to the Majlis Agama Islam Negeri is merely 5.08 percent of the 1.8 million Muslims in Johor. Based on this scenario, this study is conducted to identify factors affected the payment of zakat to the Majlis Agama Islam Negeri Johor. This will help Majlis Agama Islam Negeri Johor to focus on the main factors in increasing the number of zakat on wealth payers in Negeri Johor. This research is a fully quantitative study by using questionnaire as primary instrument to collect the data. The data was analyzed descriptively and inferential by using mean, standard deviation, one-way ANOVA, Pearson Correlation and multiple regression. SPSS 22.2 software was used in this quantitative research methods. Questionnaire instrument was developed based on modifications from previous studies. There are six variables identified in this study. Pilot study has been conducted on 30 people with Alpha-Cronbach value 0.845. About 600 survey forms were distributed in 4 zones covering 10 districts in the state of Johor. From the total, 384 questionnaires have been analyzed. The study found that the image and trust in zakat centers, knowledges about zakat, current taxes, logistics and facilities are the main factors that significantly influence to the payment of zakat in Majlis Agama Islam Negeri Johor while the faith factor and law enforcement are vice versa. This research concludes that Majlis Agama Islam Negeri Johor need to focus more specifically on the key factors to improve the collection of zakat in Johor

The Influence of Product Quality, Brand Image, and Quality of Service to Customer Trust and Implication on Customer Loyalty (Survey on Customer Brand Sharp Electronics Product at the South Kalimantan Province)

This research aims to examine the influence of: 1) examine the influence of Product Quality, Brand Image, and Quality of Service on Customer Trust, 2) Quality of Product, Brand Image, and Quality of Service on Customer Loyalty, 3) Customer Trust on Customer Loyalty Causal Research design used to examine the influence between variables. Samples taken as many as 200 Sharp Electronics customer selected accidental sampling. Data collection using questionnaires. Hypothesis testing structural models is done with GeSCA (Generalized Structured Component Analysis). The result indicate there are significant influence on Quality of Product, Brand Image and Quality of Service on Customer Trust, Quality of Product have a significant influence on Customer Loyalty, while Brand Image and Quality of service is not a significant influence on Customer Loyalty. Customer Trust have a significant influence on Customer Loyalty. Keywords: Quality of Product, Brand Image, Quality of Service, Customer Trust, and Customer Loyalty.

Nurse Self-Management Experience as a Covid-19 Vaccinator

Changes in work patterns during a pandemic will certainly impact changes in the work habits of nurses who must be able to adapt to any work situation in the field, especially for nurses vaccinated with the COVID-19 vaccine. More than 11 billion people in the world have received a dose of the Covid-19 vaccine. Data for receiving the Covid-19 vaccine in Indonesia is 208,265,720 people. Achievements in the implementation of Vaccination in Palangka Raya City reached 223,414 people with the first phase vaccine distribution of 264,173 people, the second phase amounted to 228,457 people, and the third phase vaccine reached 88,282 people. Amost 80% of nurses experienced mental health problems, namely stress during the COVID-19 pandemic. The increased workload can affect the physical and psychological health of nurses. Based on the analysis of the data, the nurse's self-management ability is needed to deal with any job demands or workload that is obtained while being a Covid-19 Vaccinator. This study aimed to explore self-management experiences among nurses as COVID-19 vaccine providers. The study used a qualitative research method with a phenomenological approach with in-depth interviews with 11 participants who served in five areas of the city of Palangka Raya and were selected based on purposive sampling inclusion criteria. The results of qualitative analysis of thematic data using the Colaizzi technique found seven themes, four of which were very relevant to self-management, namely: 1) Professional Attitude in Discrediting Tasks; 2) Coping Strategy; 3) Job Satisfaction and Altruism; 4) Teamwork Atmosphere; and three additional themes, namely: 5) Factors that cause work stress; 6) Workload of Nurses as Stressors; and 7) Holistic Response experienced by Vaccination

Optimizing Mist-Based Ablution: A Comprehensive Study of Water Distribution and Conservation Using Watercolour Visualization and Thermal Imaging Techniques

Conducting ablution constitutes a prerequisite for Muslims prior to engaging in prayer. This ritualistic practice involves the cleansing and wiping of specific body parts, including the hands, face, arms up to the elbows, head, and feet. Ensuring comprehensive water coverage of the aforementioned areas is a crucial criterion during ablution. However, excessive water consumption often occurs when Muslims perform ablution to achieve full coverage. Consequently, a more ecologically sustainable approach to ablution is necessary to minimize water wastage. A proposed water mist spray device aims to optimize water usage while adhering to the Islamic jurisprudence requirements of complete water coverage on ablution parts. To assess water coverage using the mist spray, an evenness distribution profile is employed through atomized mist colorization on paper and thermal imaging of ablution parts. An appropriate spray nozzle is chosen based on an analysis of spray distribution and coverage patterns on the target surface, utilizing image processing techniques. The proposed methodology involves mixing water with red watercolour and manually pumping it through the selected nozzle using an off-the-shelf water sprayer, thereby atomizing the coloured water to stain white paper. Subsequently, the paper is converted into a digital image and analysed using ImageJ software to determine the mist spray coverage percentage, spatial spread at various distances, and the extraction of stain and droplet sizes. This technique is applied to different types and sizes of spray nozzles to identify the most suitable nozzle for the prototype. The findings demonstrate that nozzles with smaller exit holes and higher water pressure yield more extensive spray coverage on the target surface. Upon selecting the appropriate nozzle, a Portable Ablution Mist Spray Device prototype is employed to evaluate water coverage for the ablution body parts. Thermal images of the ablution parts are captured before and after the ritual, with the temperature differences being analysed. The thermal images reveal a comprehensive and uniform spray distribution on the ablution body parts, accompanied by a temperature difference ranging from 0.9°C to 3.8°C among various participants

Optimizing Mist-Based Ablution: A Comprehensive Study of Water Distribution and Conservation Using Watercolour Visualization and Thermal Imaging Techniques

Conducting ablution constitutes a prerequisite for Muslims prior to engaging in prayer. This ritualistic practice involves the cleansing and wiping of specific body parts, including the hands, face, arms up to the elbows, head, and feet. Ensuring comprehensive water coverage of the aforementioned areas is a crucial criterion during ablution. However, excessive water consumption often occurs when Muslims perform ablution to achieve full coverage. Consequently, a more ecologically sustainable approach to ablution is necessary to minimize water wastage. A proposed water mist spray device aims to optimize water usage while adhering to the Islamic jurisprudence requirements of complete water coverage on ablution parts. To assess water coverage using the mist spray, an evenness distribution profile is employed through atomized mist colorization on paper and thermal imaging of ablution parts. An appropriate spray nozzle is chosen based on an analysis of spray distribution and coverage patterns on the target surface, utilizing image processing techniques. The proposed methodology involves mixing water with red watercolour and manually pumping it through the selected nozzle using an off-the-shelf water sprayer, thereby atomizing the coloured water to stain white paper. Subsequently, the paper is converted into a digital image and analysed using ImageJ software to determine the mist spray coverage percentage, spatial spread at various distances, and the extraction of stain and droplet sizes. This technique is applied to different types and sizes of spray nozzles to identify the most suitable nozzle for the prototype. The findings demonstrate that nozzles with smaller exit holes and higher water pressure yield more extensive spray coverage on the target surface. Upon selecting the appropriate nozzle, a Portable Ablution Mist Spray Device prototype is employed to evaluate water coverage for the ablution body parts. Thermal images of the ablution parts are captured before and after the ritual, with the temperature differences being analysed. The thermal images reveal a comprehensive and uniform spray distribution on the ablution body parts, accompanied by a temperature difference ranging from 0.9°C to 3.8°C among various participants