First record of Deraeocoris fl avilinea in Hungary (Heteroptera, Miridae: Deraeocorinae)

Deraeocoris fl avilinea (A. Costa, 1862) is recorded for the fi rst time in Hungary. All individuals were collected from the canopies of Acer pseudoplatanus, A. platanoides and A. campestre. With 2 figures

Informacijska i digitalna pismenost: Internet kao izvor informacija

Tema ovog diplomskog rada je korelacija između informacijske i digitalne pismenosti. U današnje vrijeme to znači primjena stečenih kompetencija informacijske pismenosti u računalnom odnosno internetskom okruženju. To su pojmovi koji se međusobno nadopunjuju, te su temeljna okosnica obrazovanja i procesa cjeloživotnog učenja. Cilj ovog diplomskog rada je skrenuti pažnju upravon a tu nadopunjivost ovih pojmova u digitalnomdobu. Rad je sastavljen od tri poglavlja. Prvo poglavlje u kratko predstavlja pojam informacijske pismenosti, dok drugo poglavlje govori o digitalnoj pismenosti. U trećem i zadnjem poglavlju je prikazano istraživanje informacijske i digitalne pismenosti. Ispitanici su studenti prve godine preddiplomskog studija novinarstva i politologije Fakulteta političkih znanosti u Zagrebu

ELECTRONIC DATABASES IN SOCIAL SCIENCES

Komercijalne baze podataka zbog svoje su profesionalne selektivnosti i standardizirane obrade važan izvor znanstvenih informacija. U ovom radu prikazani su razlozi njihova nastanka i razvoja u smislu tehnoloških i sadržajnih promjena kojima su on-line baze podataka bile izložene u svom četrdesetogodišnjem postojanju. Danas se na tržištu pojavljuju proizvođači hostova koji nude čitav niz baza podataka, a hrvatskoj akademskoj zajednici pristup je omogućen preko NSK-a i CARNeta. Obrađeni su primjeri pretraživanja najvažnijih on-line baza podataka trenutačno dostupnih u web-verziji (EMERALD, CSA i EBSCO) kao relevantnih elektroničkih izvora informacija iz područja društveno-humanističkih znanosti.Due to their professional selectiveness and the standardized processing, commercial databases are a valuable source of scientific information. This paper describes the rationale behind their emergence and evolution in the sense of technological and content changes that the on-line databases have gone through in their forty-year existence. On today’s market there are hosts that offer a plethora of databases. In the Croatian academic community the access is provided via NSK and CARNet. The article lists the examples of surfing on major on-line databases available in the web form (EMERALD, CSA and EBSCO) as the relevant electronic sources of information from the field of social sciences/humanities

A hagyományos citogenetika és a FISH egymást jól kiegészítő vizsgálatok gyermekkori akut limfoid leukémiában

Primary genetic abnormalities of leukemia cells have important prognostic significance in childhood acute leukemia. In the last two years 30 newly diagnosed or recurrent childhood ALL bone marrow samples were analyzed for chromosomal abnormalities with conventional G-banding and interphase-fluorescence in situ hybridization (I-FISH) using probes to detect BCR/ABL fusions, cryptic TEL/AML1 and MLL rearrangements and p16(9p21) tumor suppressor gene deletions. G-banded karyotype analysis found clonal chromosomal aberrations in 50% of cases. With the use of complementary I-FISH techniques, ALL-specific structural and numerical changes could be identified in 70% of the patients. Nine cases (30%) had subtle chromosomal aberrations with prognostic importance that had not been detected in G-banded analysis. Conventional G-banding yielded additional information (rare and complex structural aberrations) in 19% of patients. The most common aberration (30%) was AML1 copy number increase present in G-banded hyperdiploid karyotype as a chromosome 21 tetrasomy in the majority of cases; one case displayed 5-6 copies and in another case amplification of AML1 gene on der(21) was combined with TEL/AML1 fusion of the homologue AML1 gene and deletion of the remaining TEL allele. High hiperdiploidy was detected in 6 cases, in one patient with normal G-banding karyotype. TEL/AML1 fusion signals were identified in four patients. Deletion of p16 locus was found in eight cases (23%), of which only two had cytogenetically visible rearrangements. G-banding in combination with I-FISH has produced major improvements in the sensitivity and accuracy of cytogenetic analysis of ALL patients and this method helps to achieve a more precise identification of different risk categories in order to choose the optimal treatment

"guet puecher und ander dinge"

In der vorliegenden Arbeit wurden Schriftlichkeit und Verwaltungspraxis in der spätmittelalterlichen Kartause Gaming (NÖ) untersucht. In einem Überblick wurde die Gründungs des Klosters und seine Bedeutung für die Memoria des Stifterpaares Herzog Albrecht II. und Herzogin Johanna von Pfirt vorgestellt und die Verschriftlichung des Gründungsprozesses beschrieben. Für die Detailuntersuchungen zu den Schreibern der Kopialbücher und Urbare wurden die paläografischen Methoden zur Schriftbeschreibung reflektiert und an einigen Stellen weiterentwickelt. Die Entstehung der Gattungen der Diplomatare und Urbare wurde ebenso beschrieben wie der Umgang mit mittelalterlichen Massenquellen im digitalen Zeitalter, zumal die Grundlage für diese Untersuchungen die vollständige Digitalisierung des gesamten überlieferten mittelalterlichen Gaminger Archivbestandes im Wiener Haus-, Hof- und Staatsarchiv war. Die einzelnen Handschriften wurden hinsichtlich ihrer Herstellungsweise und inhaltlichen Zusammensetzung gegenübergestellt und in Bezug zu den mittelalterlichen Akteuren gesetzt. Dadurch konnte der kulturwissenschaftliche Wert von Verwaltungsquellen, ihr Informationsgehalt und Repräsentationscharakter gezeigt werden. Ihre Rezeptionsgeschichte bringt Erkenntnisse zur Kommunikationspraxis sowie die Bedeutung von ‚pragmatischer Schriftlichkeit’ für die Lebenswelt mittelalterlicher Menschen.This doctoral thesis paradigmatically investigates late-medieval writing and practices of administration in the Chartusian monastery of Gaming (Lower Austria), founded by duke Albrecht II and his wife, duchess Johanna of Pfirt. Setting out from a description of the donation of the monastery and the impact of the founders´ representational memory the study analyses the founding process with a focus on literacy. Its main part provides a detailed survey of the extant source material and a codicological as well as palaeographical analysis of chartulars and rentals demonstrating their origins as genres of late medieval administration and their importances as evidence for processes of organisation. It thoroughly reflects upon codicological and palaeographical methods, developing them further for the detailed analysis of the source material and suggesting possibilities of recording and processing the growing body of late medieval source material in the digital age. The results of these investigations are related to aspects of every-day economic and cultural practice in a medieval monastery. Thus, the quality of administrative source material regarding important questions of cultural history concerning economic decisions as well as representational politics and memory is demonstrated. The reception history of material in the 15th and 16th centuries moreover provides insights into longue durée forms of communication as well as the importance of „pragmatic literacy“ within the daily life of medieval monastic elites

ELECTRONIC DATABASES IN SOCIAL SCIENCES

Komercijalne baze podataka zbog svoje su profesionalne selektivnosti i standardizirane obrade važan izvor znanstvenih informacija. U ovom radu prikazani su razlozi njihova nastanka i razvoja u smislu tehnoloških i sadržajnih promjena kojima su on-line baze podataka bile izložene u svom četrdesetogodišnjem postojanju. Danas se na tržištu pojavljuju proizvođači hostova koji nude čitav niz baza podataka, a hrvatskoj akademskoj zajednici pristup je omogućen preko NSK-a i CARNeta. Obrađeni su primjeri pretraživanja najvažnijih on-line baza podataka trenutačno dostupnih u web-verziji (EMERALD, CSA i EBSCO) kao relevantnih elektroničkih izvora informacija iz područja društveno-humanističkih znanosti.Due to their professional selectiveness and the standardized processing, commercial databases are a valuable source of scientific information. This paper describes the rationale behind their emergence and evolution in the sense of technological and content changes that the on-line databases have gone through in their forty-year existence. On today’s market there are hosts that offer a plethora of databases. In the Croatian academic community the access is provided via NSK and CARNet. The article lists the examples of surfing on major on-line databases available in the web form (EMERALD, CSA and EBSCO) as the relevant electronic sources of information from the field of social sciences/humanities

Expression, purification, and characterization of galactose oxidase of Fusarium sambucinum in E. coli

AbstractA gene encoding a galactose oxidase (GalOx) was isolated from Fusarium sambucinum cultures and overexpressed in Escherichia coli yielding 4.4mg enzyme per L of growth culture with a specific activity of 159Umg−1. By adding a C-terminal His-tag the enzyme could be easily purified with a single affinity chromatography step with high recovery rate (90%). The enzyme showed a single band on SDS–PAGE with an apparent molecular mass of 68.5kDa. The pH optimum for the oxidation of galactose was in the range of pH 6–7.5. Optimum temperature for the enzyme activity was 35°C, with a half-life of 11.2min, 5.3min, and 2.7min for incubation at 40°C, 50°C, and 60°C, respectively. From all tested substrates, the highest relative activity was found for 1-methyl-β-galactopyranoside (226Umg−1) and the highest catalytic efficiency (kcat/Km) for melibiose (2700mM−1s−1). The enzyme was highly specific for molecular oxygen as an electron acceptor, and showed no appreciable activity with a range of alternative acceptors investigated. Different chemicals were tested for their effect on GalOx activity. The activity was significantly reduced by EDTA, NaN3, and KCN