Donor origin of circulating endothelial progenitors after allogeneic bone marrow transplantation

AbstractEndothelial cell precursors circulate in blood and express antigens found on hematopoietic stem cells, suggesting that such precursors might be subject to transplantation. To investigate, we obtained adherence-depleted peripheral blood mononuclear cells from 3 individuals who had received a sex-mismatched allogeneic bone marrow transplant (BMT) and cultured the cells on fibronectin-coated plates with endothelial growth factors. The phenotype of the spindle-shaped cells that emerged in culture was characterized by immunofluorescent staining, and the origin of the cells was determined using a polymerase chain reaction (PCR)-based assay for polymorphic short tandem repeats (STRs). The cells manifested a number of endothelial characteristics-such as von Wlllebrand factor, CD31, and Flk-1/KDR expression; Bandeiraea simplicifolia lectin 1 binding; and acetylated low-density lipoprotein uptake-but lacked expression of certain markers of activation or differentiation, including intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and the epitope for the anti-endothelial cell antibody P1H12. For each patient and at all time points studied (ranging from 5 to 52 months after transplantation), STR-PCR analysis showed that cultured cells and nucleated blood cells came exclusively from the bone marrow donor. These results demonstrate that circulating endothelial progenitors are both transplantable and capable of long-term repopulation of human allogeneic BMT recipients.Biol Blood Marrow Transplant 2000;6(3A):301-8

Blueberry Research Progress Reports

The 1989 edition of the Blueberry Research Progress Reports was prepared for the Maine Wild Blueberry Commission and the University of Maine Wild Blueberry Advisory Committee by researchers with the Maine Agricultural Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include: 1. Control of Secondary Blueberry Pests 2. Monitory Methods, Economic Injury Levels, and Action Thresholds of Secondary Blueberry Pests 3. Control of Blueberry Maggot 4. Phosphorus Dose/Response Curve 5. Nitrogen-Phosphorus Study 6. Multiple Cropping of Wild Stands 7. Changes in Sugars and Organic Acids of Blueberries During Development 8. Investigation of Preprocess Changes That Could Lead to the Development of a Simple and Inexpensive Method to Measure Preprocess Berry Spoilage 9. Development of Simple and Less Expensive Methods to Analyze Pesticides Used on Maine and Canadian Blueberries 10. Evaluation and Modification of Commercial Wipers 11. Effect of Rate and Formulation of Hexazinone on Bunchberry 12. Bracken Fern Control Alternatives 13. Evaluation of Hexazinone with Spot Treatments of Glyphosate Sethoxydim or Fluazifop-P for Bunchgrass Control 14. Directed Sprays of Glyphosate for Bunchberry Control 15. Evaluation of Norflurazon with or without Hexazinone for Bunchgrass Control 16. Selective Wiper and Mechanical Control of Dogbane 17. Evaluation of Sulfonyl Urea Herbicides for Bunchberry Control 18. Seedling Pruning Study 19. Evaluation of Sethoxydim in Lowbush Blueberry Fields 20. Blueberry Extension Program 21. Pollination of the Low-bush Blueberry by Native Bees 22. Postharvest Fungi of Lowbush Blueberries 23. Effects of Pruning Methods on Mummy Berry Incidenc

Roles of spatial scale in quantifying stock-recruitment relationships for American lobsters in the inshore Gulf of Maine

It is well known in ecological studies that the choice of spatial scale can influence the possibility of detecting ecological patterns and the type of patterns observed. However, this has rarely been evaluated for fish stock-recruitment (SR) dynamics. Inappropriate scales may result in failure to identify possible stock-recruitment relationships, especially for species with complicated life history and stock structure and locally generated recruitment. Using American lobster in the Gulf of Maine (GOM) as an example, we tested the hypotheses that the SR relationship is detectable only at certain spatial scales and the functional SR relationships vary with spatial scales. We estimated the SR relationship separately for American lobster in the eastern and western GOM, which have strongly differing oceanographic conditions that may result in different suitable spatial scale and SR dynamics for lobster. We analyzed data of 11 different spatial scales using a Bayesian method. The model fit and performances in the posterior predictive assessment for the SR models were convexly related to the spatial scales. The functional SR relationships differed for different spatial scale. The SR parameter estimates are negatively or concavely related to the spatial scale. The best model was found at medium spatial scale for both the eastern and western GOM and the scale differed between the eastern and western GOM, suggesting that optimal spatial scale might be process-related. We demonstrated that the choice of spatial scale directly affected the possibility of identifying the SR relationship, the estimation of SR parameters, the type of SR relationships and the predictive abilities of the SR models.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

The Acute Effects of Loaded Jump on Vertical Jump Performance and Perception

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The Acute Effects of Loaded Jump on Vertical Jump Performance and Perception

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The Relationship Between Labor Market Structure and Clergy Compensation in Protestant Denominations

Labor markets, Economic analysis of religion, Economic justice, I30, J31, J42, L14, L22, Z12,