A new species of Colletes (Hymenoptera: Apoidea: Colletidae) from northern Florida and Georgia, with notes on the Colletes of those states

Colletes ultravalidus Hall & Ascher, new species, is described from several sites in northwestern Florida and southeastern Georgia.  It is a member of the inaequalis species group, very similar to C. validus Cresson, a specialist of Ericaceae, but can be distinguished by an even more elongate malar area and the absence of conspicuous tergal fascia.  Colletes ultravalidus has been found flying from early winter to early spring when it forms nest aggregations in xeric sites adjacent to shrub bog or basin swamp, the habitat of Pieris phyllyreifolia (Hook.) DC. (Ericaceae), the most likely, but as yet unconfirmed, host plant of the new species.  State records of Colletes for Florida and Georgia are reviewed and discrepancies in taxonomy and distributional limits between Stephen’s 1954 revision of the genus and Mitchell’s 1960 monograph of eastern North American bees are noted.  We concur with Stephen that the distributions of several taxa in Colletes are more limited than that reported by Mitchell

The percutaneous absorption of soman in a damaged skin porcine model and the evaluation of WoundStat™ as a topical decontaminant

PURPOSE: The aim of this study was to evaluate a candidate haemostat (WoundStat™), down-selected from previous in vitro studies, for efficacy as a potential skin decontaminant against the chemical warfare agent pinacoyl methylfluorophosphonate (Soman, GD) using an in vivo pig model. MATERIALS AND METHODS: An area of approximately 3 cm2 was dermatomed from the dorsal ear skin to a nominal depth of 100 µm. A discrete droplet of 14C-GD (300 µg kg-1) was applied directly onto the surface of the damaged skin at the centre of the dosing site. Animals assigned to the treatment group were given a 2 g application of WoundStat™ 30 s after GD challenge. The decontamination efficacy of WoundStat™ against GD was measured by the direct quantification of the distribution of 14C-GD, as well as routine determination of whole blood cholinesterase and physiological measurements. RESULTS: WoundStat™ sequestered approximately 70% of the applied 14C-GD. Internal radiolabel recovery from treated animals was approximately 1% of the initially applied dose. Whole blood cholinesterase levels decreased to less than 10% of the original value by 15 min post WoundStat™ treatment and gradually decreased until the onset of apnoea or until euthanasia. All treated animals showed signs of GD intoxication that could be grouped into early (mastication, fasciculations and tremor), intermediate (miosis, salivation and nasal secretions) and late onset (lacrimation, body spasm and apnoea) effects. Two of the six WoundStat™ treated animals survived the study duration. CONCLUSIONS: The current study has shown that the use of WoundStat™ as a decontaminant on damaged pig ear skin was unable to fully protect against GD toxicity. Importantly, the findings indicate that the use of WoundStat™ in GD contaminated wounds would not exacerbate GD toxicity. These data suggest that absorbent haemostatic products may offer some limited functionality as wound decontaminants.Peer reviewedFinal Accepted Versio

Destructive physical analysis results of Ni/H2 cells cycled in LEO regime

Six 48-Ah individual pressure vessel (IPV) Ni/H2 cells containing 26 and 31 percent KOH electrolyte were life cycle tested in low Earth orbit. All three cells containing 31 percent KOH failed (3729, 4165, and 11,355 cycles), while those with 26 percent KOH were cycled over 14,000 times in the continuing test. Destructive physical analysis (DPA) of the failed cells included visual inspections, measurements of electrode thickness, scanning electron microscopy, chemical analysis, and measurements of nickel electrode capacity in an electrolyte flooded cell. The cycling failure was due to a decrease of nickel electrode capacity. As possible causes of the capacity decrease, researchers observed electrode expansion, rupture, and corrosion of the nickel electrode substrate, active material redistribution, and accumulation of electrochemically undischargeable active material with cycling

Student satisfaction : impact of age and gender on satisfaction among international postgraduate students from Asia

Customer satisfaction is a core-marketing concept. It is considered as a major outcome of marketing activity and serves to link processes, culminating in purchase and consumption with post-purchase phenomena such as attitudinal change, customer retention, repeat purchase, brand loyalty, positive word-of-mouth communication. Student satisfaction is a strategic factor in developing a competitive advantage in the highly attractive and globally competitive international education market. Market forecasts indicate a seven-fold increase in the number of students seeking higher education overseas by 2025 and given the competitiveness of the industry, universities will need to focus on variables influencing student satisfaction in order to address areas where improvements in service quality are required.A sample of 371 postgraduate students from China, India, Indonesia and Thailand, is investigated. The study highlights the development of a scale to measure international postgraduate student satisfaction. The scale demonstrates the importance of four predominant factors influencing university choices - Education Resources; Communication and Guidance; Customer Value and Study Outcomes; and Image, Prestige and Recognition. Using logistic regression and chi square testing, this paper investigates the impact of age and gender on satisfaction among international postgraduate students from four Asian countries studying in universities in Victoria, Australia, on theses factors. The results indicate that age has a positive relationship with satisfaction among postgraduate students while the influence of gender has no effect on influencing satisfaction among postgraduate students from Asia

Direct Boundary Element Method for Dynamics in a Half-Space

An application of the direct boundary element method for solving the response of a linearly elastic half-space with a canyon cut into the surface is presented. This approach uses source solutions for an undamped half-space where the resulting singular integral equations are solved directly without adding any artificial damping. Solutions for the displacements on the canyon surface reveal an artificial resonance phenomenon when solving the exterior problem in the frequency domain. The use of an additional source loading in the boundary element method is shown to eliminate these resonances and yield accurate results. A method for solving the Rayleigh waves generated on the surface of the half-space caused by the canyon is shown

Distribution, Seasonality and Abundance of King and Spanish Mackerel Larve in the Northwestern Gulf of Mexico (Pisces: Scombridae)

Larvae of king mackerel, Scomberomorus cavalla, and Spanish mackerel, S. maculatus were collected from 1975 through 1977 off the Texas coast. Both species were captured from May through October. S. cavalla was relatively more abundant of the two species and occurred most abundantly over the middle and outer continental shelf (35-183 m). At least 35% of the larvae were captured in September of each year. S. maculatus larvae occurred most abundantly over the inner continental shelf (12 to 50 m). S. cavalla spawned from May through September to early October, with the greatest spawning intensity occurring over the middle and outer continental shelf during September. S. maculatus spawned from May through September to early October over the inner continental shelf, but spawning was less intensive and more irregular than for S. cavalla. Comparisons with other larval studies of S. cavalla and S. maculatus suggest that the northwestern and northeastern Gulf of Mexico and the coast off the southeastern United Stales are important spawning areas for S. cavalla and that the eastern and northeastern Gulf of Mexico are important spawning areas for S. maculatus

Evaluation of a novel assay for detection of the fetal marker RASSF1A: facilitating improved diagnostic reliability of noninvasive prenatal diagnosis

BackgroundAnalysis of cell free fetal (cff) DNA in maternal plasma is used routinely for non invasive prenatal diagnosis (NIPD) of fetal sex determination, fetal rhesus D status and some single gene disorders. True positive results rely on detection of the fetal target being analysed. No amplification of the target may be interpreted either as a true negative result or a false negative result due to the absence or very low levels of cffDNA. The hypermethylated RASSF1A promoter has been reported as a universal fetal marker to confirm the presence of cffDNA. Using methylation-sensitive restriction enzymes hypomethylated maternal sequences are digested leaving hypermethylated fetal sequences detectable. Complete digestion of maternal sequences is required to eliminate false positive results.MethodscfDNA was extracted from maternal plasma (n = 90) and digested with methylation-sensitive and insensitive restriction enzymes. Analysis of RASSF1A, SRY and DYS14 was performed by real-time PCR.ResultsHypermethylated RASSF1A was amplified for 79 samples (88%) indicating the presence of cffDNA. SRY real time PCR results and fetal sex at delivery were 100% accurate. Eleven samples (12%) had no detectable hypermethylated RASSF1A and 10 of these (91%) had gestational ages less than 7 weeks 2 days. Six of these samples were male at delivery, five had inconclusive results for SRY analysis and one sample had no amplifiable SRY.ConclusionUse of this assay for the detection of hypermethylated RASSF1A as a universal fetal marker has the potential to improve the diagnostic reliability of NIPD for fetal sex determination and single gene disorders

Associations of blood pressure with body composition among Afro-Caribbean children in Barbados

Despite complex presentation of adult hypertension and a concomitant obesity epidemic, little is known about overweight in relation to blood pressure among Caribbean children. We examined blood pressure in relation to body size in a cross-sectional study of 573 Barbadian children aged 9–10 years (2010-2011).The United States normative blood pressure percentiles were used to identify children with high (≥ 95th percentile) or high normal blood pressure (90th – 95th percentile). The World Health Organization body mass index cut-off points were used to assess weight status. Major findings: Thirty percent of children were overweight/obese. Percentage fat mass differed between girls (20.4%) and boys (17.72%) (p< 0.05). Mean systolic blood pressure among girls was 106.11 (95% CI 105.05, 107.17) mmHg and 105.23 (104.09, 106.38) for boys. The percentages with high or high-normal mean systolic blood pressurewere14.38% (10.47, 18.29) for girls and 8.08% (4.74, 11.41) for boys. Height and body mass index were independent correlates of systolic and diastolic blood pressure. Mean systolic blood pressure was related to lean mass but not fat mass, while diastolic blood pressure was associated with fat mass index and overweight. Principal conclusion: One third of 9-10 year old children in Barbados were overweight/obese and 12% had elevated mean systolic blood pressure. BP was related to body size. These findings signal potential adverse trends in weight gain and BP trends for children growing up in the context of a country that has recently undergone rapid economic transition

Stellar Encounters with Massive Star-Disk Systems

The dense, clustered environment in which massive stars form can lead to interactions with neighboring stars. It has been hypothesized that collisions and mergers may contribute to the growth of the most massive stars. In this paper we extend the study of star-disk interactions to explore encounters between a massive protostar and a less massive cluster sibling using the publicly available SPH code GADGET-2. Collisions do not occur in the parameter space studied, but the end state of many encounters is an eccentric binary with a semi-major axis ~ 100 AU. Disk material is sometimes captured by the impactor. Most encounters result in disruption and destruction of the initial disk, and periodic torquing of the remnant disk. We consider the effect of the changing orientation of the disk on an accretion driven jet, and the evolution of the systems in the presence of on-going accretion from the parent core.Comment: 11 pages, 10 figures, accepted to Ap