7 research outputs found
A humán parechovírusok klinikai jelentősége súlyos újszülött- és csecsemőkori fertőzésekben hazánkban = The clinical significance of severe human parechovirus infections in newborns and infants in Hungary
Absztrakt:
Bevezetés: A humán parechovírus (HPeV,
Picornaviridae víruscsalád) okozta fertőzések többsége
tünetmentes, de gyermekeknél hasmenéssel járhatnak. Újabb megfigyelés, hogy a
HPeV-ok a csecsemők körében súlyos, központi idegrendszeri megbetegedést és
szepszisszerű kórképet is okozhatnak. A HPeV-ok szerepe a hazai klinikumban nem
ismert. Célkitűzés: A HPeV-ok kimutatása, meghatározása
gyermekektől származó székletmintákból és a klinikai tünetek elemzése.
Módszer: A HPeV-ok kimutatása és tipizálása a reverz
transzkripció–polimeráz-láncreakció és a szekvenálás módszereivel történt három
betegcsoportban: A) 10 év alatti kórházban kezelt, n = 75; B) 1 év alatti, n =
237; és C) szisztémás/idegrendszeri tüneteket is mutató hasmenéses gyermekek
(<18 év), n = 105 székletmintáiból. Eredmények: Az A
csoportban 4% (3/75) volt a HPeV-pozitív minták aránya (két HPeV1, egy nem
tipizálható HPeV), mind gastroenteralis tüneteket mutató csecsemőkből (7–11
hónapos). A B csoportban a HPeV-fertőzések aránya 6,8% (16/237) volt, melyek
közül öt HPeV1, hat HPeV3 és öt nem tipizálható HPeV volt. E csoportban a
HPeV1-fertőzések többsége (4/5) nem igényelt kórházi ellátást, míg a
HPeV3-fertőzéses esetek 83%-a (5/6) igényelte azt. A szisztémás/idegrendszeri
tüneteket is mutató betegekből (C csoport) azonosított HPeV-pozitív minták
(4,8%) két genotípusba tartoztak (három HPeV1 és két HPeV3), és mind kórházban
kezelt fiatal (≤2 hónapos) csecsemőktől származtak.
Következtetés: A HPeV1-fertőzések általában enyhébb
lefolyásúak, és ritkábban igényelnek kórházi ellátást. A HPeV1-fertőzés vezető
tünete a hasmenés, azonban 1–2 hónapos csecsemőknél idegrendszeri érintettség
(aluszékonyság, bágyadtság) is tapasztalható volt. Az újszülöttek körében a
HPeV3-megbetegedések gyakoribbak. A súlyos HPeV3-fertőzésre jellemzőek a
gastroenteralis tünetek (7/8), a ≥38 °C-os láz (6/7), az étvágytalanság (6/7),
az exanthema (4/7), az aluszékonyság/bágyadtság (3/7), a szepszisszerű állapot
(3/7) és a légzőszervi tünetek (2/7). Orv Hetil. 2019; 160(10): 386–395.
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Abstract:
Introduction: Most human parechovirus (HPeV, family
Picornaviridae) infections are asymptomatic but may cause
gastroenteritis in children. New reports show that HPeVs can be associated with
severe central nervous system symptoms and sepsis-like syndromes in infants. The
clinical significance of HPeVs in Hungary has not been investigated before.
Aim: The aim of this study was to detect genotype HPeV in
faecal samples of children and analysis of the clinical symptoms.
Method: For the detection and genotyping of HPeV strains,
reverse transcription–polymerase chain reaction and sequencing methods were used
from faecal samples of children with gastroenteritis divided into three groups:
group A) hospitalised children younger than 10 years (n = 75); group B) 0–12
months infants (n = 237) and group C) children less than 18 years of age with
sepsis-like/neurological symptoms (n = 105) were tested.
Results: Three HPeV positive samples (3/75, 4%) were found
in group A, two of them belong to the HPeV type 1, the third was non-typeable.
All positive samples were from infants of 7 to 11 months of age. In group B,
HPeV was detected in 6.8% (16/237) of the samples. Five were HPeV1, six were
HPeV3 and five were non-typeable. While most of the infants with HPeV1 (4/5) did
not require hospitalisation, 83% of the HPeV3 infected infants (5/6) did. Five
(4.8%) HPeV strains detected from children less than 18 years of age with
sepsis-like/neurological symptoms (group C) belonged to HPeV1 (three) and HPeV3
(two). All positive samples were from hospitalised infants less than 2 months of
age. Conclusion: HPeV1 infections are less severe in infants
than HPeV3 infections. The leading symptom of HPeV1 was diarrhoea, although in
infants less than 1–2 months neurological symptoms (somnolence, lassitude) were
also present. HPeV3 infections were more common among newborns. The main
symptoms of severe HPeV3 infection are: gastroenteritis (7/8), fever ≥38 °C
(6/7), loss of appetite (6/7), rash (4/7), somnolence/lassitude (3/7),
sepsis-like syndrome (3/7) and respiratory symptoms (2/7). Orv Hetil. 2019;
160(10): 386–395
Virulence Traits of Inpatient Campylobacter jejuni Isolates, and a Transcriptomic Approach to Identify Potential Genes Maintaining Intracellular Survival
There are still major gaps in our understanding of the bacterial factors that influence the outcomes of human Campylobacter jejuni infection. The aim of this study was to compare the virulence-associated features of 192 human C. jejuni strains isolated from hospitalized patients with diarrhoea (150/192, 78.1%), bloody diarrhoea (23/192, 11.9%), gastroenteritis (3/192, 1.6%), ulcerative colitis (3/192, 1.5%), and stomach ache (2/192, 1.0%). Traits were analysed with genotypic and phenotypic methods, including PCR and extracellular matrix protein (ECMP) binding, adhesion, and invasion capacities. Results were studied alongside patient symptoms, but no distinct links with them could be determined. Since the capacity of C. jejuni to invade host epithelial cells is one of its most enigmatic attributes, a high throughput transcriptomic analysis was performed in the third hour of internalization with a C. jejuni strain originally isolated from bloody diarrhoea. Characteristic groups of genes were significantly upregulated, outlining a survival strategy of internalized C. jejuni comprising genes related (1) to oxidative stress; (2) to a protective sheath formed by the capsule, LOS, N-, and O- glycosylation systems; (3) to dynamic metabolic activity supported by different translocases and the membrane-integrated component of the flagellar apparatus; and (4) to hitherto unknown genes
Multiple divergent picobirnaviruses with functional prokaryotic Shine- Dalgarno ribosome binding sites present in cloacal sample of a diarrheic chicken
Picobirnaviruses (PBVs) of family Picobirnaviridae have bisegmented (S1 and S2 segments), doublestranded RNA genomes. In this study a total of N = 12 complete chicken PBVs (ChPBV) segments (N =
5 of S1 and N = 7 of S2, Acc. Nos.: MH425579-90) were determined using viral metagenomic and RTPCR techniques from a single cloacal sample of a diarrheic chicken. The identified ChPBV segments are
unrelated to each other and distant from all of the currently known PBVs. In silico sequence analyses
revealed the presence of conserved prokaryotic Shine-Dalgarno-like (SD-like) sequences upstream of the
three presumed open reading frames (ORFs) of the S1 and a single presumed ORF of the S2 segments.
According to the results of expression analyses in E. coli using 6xHis-tagged recombinant ChPBV
segment 1 construct and Western blot these SD-like sequences are functional in vivo suggesting that S1
of study PBVs can contain three ORFs and supporting the bacteriophage-nature of PBVs