Investigation of survival ratio and growth rate in offspring of Salmo trutta caspius

We investigated the survival ratio and the growth rate of offspring of Salmo tuna caspius produced from three groups of brood fish in Shahid Bahonar Rearing and Breeding Center in Klardasht in 2005. The three groups of brood fish belonged the early, mid, and late-migration period. The sampling was done randomly, and in each group 15 female and 5 male fish were included. After spawning and hatching, 22500 eggs were used in each group (3 treatments, repeated 9 times). The results showed no significant difference in the survival ratio of the egg and larva in the three groups of the brood fish in each stage of evolution up to two months after the start of active feeding (P>0.05). However, after the start of active feeding there was a significant difference (P 0.05). Based on the results, we suggest that the brood fish from the early migration period are more suitable for producing offspring and their offspring pass through the smoltification stage more rapidly. However, in terms of genetic diversity, it would be appropriate to use brood fish from all migration periods

The lethal concentration (LC50) of Zataria multiflora essential oil in fries of rainbow trout (Oncorhynchus mykiss)

This research was carried out to determine LC50 for Zataria multiflora essential oil in rainbow trout (Oncorhynchus mykiss) fries at 96 hours. In the process, we used the static O.E.C.D method for 180 rainbow trout fries weighing 5±1 grams in five treatments and one control with three replicates. During the experiments, the mean (±SD) of water temperature was kept at 16±1 C, dissolved oxygen (DO) was 7±0.2mg/l and pH was 8±0.4. We used 5- 25ppm of the essential oil and the results were analyzed using probit analysis procedure. The LC50 96h of Zataria multiflora essential oil was determined as 13.6ppm for rainbow trout (Oncorhynchus mykiss) fry. Based on the results, the useful maximum concentration of Zataria multiflora essential oil was less than 10ppm in rainbow trout fry. The results indicated a narrow therapeutic index for Zataria multiflore essential oil

A study of the anesthetic effect of Zataria multiflora Boiss (Labiatae) essence on Oncorhynchus mykiss and cultured Salmo trutta caspius

For the first time in the world, the effect of Zataria multiflora essence in concentrations of 50 and 100 ppm on Oncorhynchus mykiss fry weighing on average 15±2 g and cultured Salmo trutta caspius weighing on average 40±4 g was studied. The water temperature was 16-17 °C, dissolved oxygen was 7±0.2 and pH was 8 during the investigation. O. mykiss and S. trutta caspius reached complete sedation during an average time of 3 and 2 minutes respectively in the two concentrations. The mean balance and recovery time were 2 and 3 minutes respectively. Results showed that the sedation and recovery time are significantly different in the two species and with the two concentrations of the sedative (P<0.05). The research also showed that Z. multiflora essence has an anesthetic effect on the two species, but because of the unwanted severe irreparable breathing side effects and the accompanied body deformation of the two species, the essence is not suggested as an anesthetic for the fish. The essence is not recommended as a general anesthetic, but it may be used in 15-20ppm as a sedative during manipulation of the fish

Evaluation of WHO’s partogram alert line for prediction of the APGAR score at the first minute after birth

Background and aim: The early diagnosis of abnormal progress of labour and prevention of long term delivery are the main keys to reduce fetal mortality rate due to hypoxia and tissue ischemia. One of the WHO’s proposed methods for prevention of abnormal delivery is using partogram. Therefore, this survey was conducted to evaluate WHO’s partogram alert line for APGAR score at the first minute after birth. Methods: This descriptive–analytical study was performed on 140 pregnant women under partogram in Shahrood Fatemieh hospital. Using the partogram alert line, the progress of labour, mother’s and infants condition started to be controled from 3 cm dilation of the cervix. Decision for resuscitation was made based on respiration, heart rate and skin color of the infant 20 to 30 seconds after the birth. Tools of this research were a questionare, inspection form consisted of WHO’s partogram and step by step resuscitation form. χ2, t–test and analytical methods of sensitivity, specificity and negative and positive values were used for data analysis. Results: The results showed that 68.8% of the subjects cervical dilatation remained on or to the left side of the alert line and 28.6% was on the right side and 2.9% was on or to the right side of the active line. According to χ2 test, there was a significant relationship between the first minute APGAR score after delivery and the location of cervical dilation. The value of alert line for surveying of APGAR score at the first minute after birth had a sensitivity of 25%, specificity of 96.87% , negative and positive predictive value of 75.6% and 76.92%, respectively. Conclusion: Our results showed that using WHO’s partogram prevents long term delivery, helps for on time diagnosis of fetal distress and reduction in stillbirth and fetal mortality. So, partogram should be a part and parcel of fetal and maternal health care

Evaluation and comparison of clinical and para clinical diagnosis of Candida vaginitis in women referred to Shahrood city health care centers years between 2004-2007

Background and aim: Candida vaginitis is the second common cause of vaginitis and Candida albicans is cause of 90 percent of vaginal fungal infections. Because antifungal drugs are among the cheapest vaginal drugs this study was performed to evaluate and compare clinical and paraclinical diagnosis of candida vaginitis in women referred to health care centers in Shahrood cityfrom 2004 to 2007. Methods: This deh1ive-analytic research was performed on 300 women with vaginitis infections who were referred to the Shahrood city health care centers. Two samples of secretions were taken from both endocervix and exocervix using speculum and spread on two glass slides and then one sample was taken from posterior culdesac secretions with a cotton soup and entered into a tube containing sterilized physiological serum. A questionnaire was filled out by a midwife for every patient. Standard laboratory methods (cultures Pap smear wet smear and etc.) were used to evaluate the samples and the second questionnaire was filled out by the midwife. Finally results were analyzed by statistical methods. Results: Based on clinical results 132 persons (44%) had candida infection but the infection was not proved by cell culture in 30 patients ((10%) and wet smear in 111 persons (37%). No candida infection was found in cytology survey. Based on clinical results vaginal pH was measured 5.01. The amount of pH was found 5.52 5.8 and 5.71 based on wet smear cell cultures and Pap smear respectively. The physical examination sensitivity for detecting vaginal candidiasis was 63.33% compared to the cell culture. Conclusion: The results of this study show that some para-clinical examinations should be considered for diagnosis of vaginal candidiasis. These para-clinical experiments help to prevent and reduce the side effects of some unnecessary medications

Molecular study of PKD1 &amp; PKD2 genes by linkage analysis and determining the genotype/phenotype correlations in several Iranian families with autosomal dominant polycystic kidney disease

Background: Autosomal dominant polycystic kidney disease (ADPKD) is an inherited disorder with genetic heterogeneity. Up to three loci are involved in this disease, PKD1 on chromosome 16p 13.3, PKD2 on 4q21, and a third locus of unknown location. Methods: Here we report the first molecular genetic study of ADPKD and the existence of locus heterogeneity for ADPKD in the Iranian population by performing linkage analysis on 15 affected families. Results: Eleven families showed linkage to PKD1 and two families showed linkage to PKD2. In two families, PKD1 markers are common in all affected members but PKD2 markers were not informative. Conclusion: The results of this study demonstrate significant locus heterogeneity in autosomal dominant PKD in Iran. Analysis of clinical data confirms a milder ADPKD phenotype for PKD2 families. Our results showed relatively high heterozygosity rates and PIC values for some markers, while the most informative markers were KG8 and 16AC2.5 for PKD1 gene and AFM224x6 for PKD2 gene

Gain properties of dye-doped polymer thin films

Hybrid pumping appears as a promising compromise in order to reach the much coveted goal of an electrically pumped organic laser. In such configuration the organic material is optically pumped by an electrically pumped inorganic device on chip. This engineering solution requires therefore an optimization of the organic gain medium under optical pumping. Here, we report a detailed study of the gain features of dye-doped polymer thin films. In particular we introduce the gain efficiency $K$, in order to facilitate comparison between different materials and experimental conditions. The gain efficiency was measured with various setups (pump-probe amplification, variable stripe length method, laser thresholds) in order to study several factors which modify the actual gain of a layer, namely the confinement factor, the pump polarization, the molecular anisotropy, and the re-absorption. For instance, for a 600 nm thick 5 wt\% DCM doped PMMA layer, the different experimental approaches give a consistent value $K\simeq$ 80 cm.MW$^{-1}$. On the contrary, the usual model predicting the gain from the characteristics of the material leads to an overestimation by two orders of magnitude, which raises a serious problem in the design of actual devices. In this context, we demonstrate the feasibility to infer the gain efficiency from the laser threshold of well-calibrated devices. Besides, temporal measurements at the picosecond scale were carried out to support the analysis.Comment: 15 pages, 17 figure

The effect of different feeding frequency on growth indices, survival and body composition of Oriental River Prawn Macrobrachium nipponense (De Haan, 1849)

This research was carried out in order to identify the effects of different feeding frequency on growth indices, feed conversion ratio, survival and body composition of oriental river prawn (Macrobrachium nipponense) during 56 days. 180 oriental river prawns, with mean (±SE) weight 1.40±0.11g were counted and distributed randomly in twelve 70-liter glass tanks with 60 l water in 4 treatments each with 3 replicates cultured in same conditions (dietary feeding, dissolved oxygen, photoperiod, density, 2% feeding rate). Feeding were done 3, 4, 5 and 6 times/day with a constant commercial diet (50% protein, 13% fat, 12% ash, moisture 10-12 %). At the end of experiment, growth indices, feed conversion ratio, body composition ratio were measured for each treatment. Mean final weight, weight gain and specific growth rate in the treatment feeding 5 times/day was higher than the other treatments (p0.05).The lowest feed conversion ratio was obtained in 5 times/day feeding and feeding 4 and 5 times/day treatments (68.80±1.8 and 77.60±2.5 %, respectively) had the highest survival rate. The highest body protein content (12.86±0.07 %) and fat content (3.46±0.02%) were obtained from the 5 times/day feeding. Results indicate that 5 times/day feeding has a significant difference in the increase of growth indices, survival rate, reduced feed conversion ratio, increased carcass protein and fat content, with other treatments (p<0.05)