Quantitative (Non-qualitative) Changes of IgG Anti-Mumps After Freeze-‎Thaw Cycles

Object. The result of freeze-thaw (FT) tested sera is always doubtful, and is a matter of question which is accompanied by false negative results. Aim: To determine the effect of repeated FT cycles, on the assay results of mumps IgG antibody. Material and Methods. This prospective laboratory study includes 25 volunteers. 25 sera underwent tests for first Mumps IgG antibody, and then were frozen at -20 degrees centigrade. One week later, they were thawed and stored again at 4 degrees centigrade for one more week when the second assays, were performed. The results for mumps IgG ELISA as an effect of two FT cycles and storage temperature were reported. The results were recorded by first and second Immune Serum Ratio (ISR) value (for first and second assay) and the percentage change of ISR. A linear regression model applied for analyzing ISR change percentage. Results. There were three kinds of ISR value change as: 1-The stable ISR value (44%). 2-The decreasing change of ISR value (32%) mainly among males, older in age (14-19 years) and the first ISR more than the value of four. 3-The increasing change of ISR value (24%) which occurred in males 10-12 years with the first ISR value of 1-2 (lowest concentration). Based on regression model, level of the first ISR and group of test were significant factor for change percentage of ISR, but neither gender nor age, were significant. Discussions. Mumps IgG antibody , as a function of two FT cycles, was affected by quantitative but not qualitative alteration. If the first ISR has value of 3-4, most probably it wills no any significant changes due to at least two FT cycles

The lag and duration-luminosity relations of gamma-ray burst pulses

Relations linking the temporal or/and spectral properties of the prompt emission of gamma-ray bursts (hereafter GRBs) to the absolute luminosity are of great importance as they both constrain the radiation mechanisms and represent potential distance indicators. Here we discuss two such relations: the lag-luminosity relation and the newly discovered duration-luminosity relation of GRB pulses. We aim to extend our previous work on the origin of spectral lags, using the duration-luminosity relation recently discovered by Hakkila et al. to connect lags and luminosity. We also present a way to test this relation which has originally been established with a limited sample of only 12 pulses. We relate lags to the spectral evolution and shape of the pulses with a linear expansion of the pulse properties around maximum. We then couple this first result to the duration-luminosity relation to obtain the lag-luminosity and lag-duration relations. We finally use a Monte-Carlo method to generate a population of synthetic GRB pulses which is then used to check the validity of the duration-luminosity relation. Our theoretical results for the lag and duration-luminosity relations are in good agreement with the data. They are rather insensitive to the assumptions regarding the burst spectral parameters. Our Monte Carlo analysis of a population of synthetic pulses confirms that the duration-luminosity relation must be satisfied to reproduce the observational duration-peak flux diagram of BATSE GRB pulses. The newly discovered duration-luminosity relation offers the possibility to link all three quantities: lag, duration and luminosity of GRB pulses in a consistent way. Some evidence for its validity have been presented but its origin is not easy to explain in the context of the internal shock model.Comment: 8 pages, 5 figures, 1 tabl

A highly sensitive multiplexed FBG pressure transducer based on natural rubber diaphragm and ultrathin aluminium sheet

Pressure measurement with a good sensitivity has always been a concern in most of the engineering applications and biomedical field. In this paper, a multiplexed FBG bonded on an ultrathin aluminium sheet which act as a cantilever deflected due to a deformation from a natural rubber based diaphragm has been proposed and studied. By using two gratings inscribed on a single optical fibre which senses the positive and negative strain has enhanced the sensitivity of the pressure transducer recorded at 329.56 pm/kPa or corresponding to 10.7893 kPa−1 across the range of 0 to 10 kPa with a good linearity of 99.76%. Furthermore, the thermal cross-sensitivity is compensated

Axl-EGFR receptor tyrosine kinase hetero-interaction provides EGFR with access to pro-invasive signalling in cancer cells

© The Author(s) 2016. Acquired resistance to conventional and targeted therapies is becoming a major hindrance in cancer management. It is increasingly clear that cancer cells are able to evolve and rewire canonical signalling pathways to their advantage, thus evading cell death and promoting cell invasion. The Axl receptor tyrosine kinase (RTK) has been shown to modulate acquired resistance to EGFR-targeted therapies in both breast and lung cancers. Glioblastoma multiforme (GBM) is a highly infiltrative and invasive form of brain tumour with little response to therapy. Both Axl and EGFR have been identified as major players in gliomagenesis and invasiveness. However, the mechanisms underlying a potential signalling crosstalk between EGFR and Axl RTKs are unknown. The purpose of this study was to investigate this novel and unconventional interaction among RTKs of different families in human GBM cells. With the use of western blotting, in vitro kinase activity, co-immunoprecipitation and bimolecular fluorescence complementation assays, we show that EGF stimulates activation of Axl kinase and that there is a hetero-interaction between the two RTKs. Through small interfering RNA knockdown and quantitative PCR screening, we identified distinct gene expression patterns in GBM cells that were specifically regulated by signalling from EGFR-EGFR, Axl-Axl and EGFR-Axl RTK parings. These included genes that promote invasion, which were activated only via the EGFR-Axl axis (MMP9), while EGFR-EGFR distinctly regulated the cell cycle and Axl-Axl regulated invasion. Our findings provide critical insights into the role of EGFR-Axl hetero-dimerisation in cancer cells and reveal regulation of cell invasion via Axl as a novel function of EGFR signalling

Heavy Military Land Vehicle Mass Properties Estimation Using Hoisting and Pendulum Motion Method

Mass properties such as the centre of gravity location, moments of inertia, and total mass are of great importance for vehicle stability studies and deployment. Certain parameters are required when these vehicles need to be arranged inside an aircraft for the carrier to achieve proper mass balance and stability during a flight. These parameters are also important for the design and modelling process of vehicle rollover crash studies. In this study, the mass properties of a military armoured vehicle were estimated using hoisting and pendulum method. The gross total weight, longitudinal and vertical measurements were recorded by lifting the vehicle using a mobile crane and the data were used to estimate the centre of gravity. The frequency of vehicle oscillation was measured by applying swing motion with a small angle of the vehicle as it is suspended on air. The centre of gravity and mass moment of inertia were calculated using the vector mechanics approach. The outcomes and limitations of the approach as discussed in details

PROTECT: Relational Safety based Suicide Prevention Training Frameworks

Preventing suicide is a global priority and staff training is a core prevention strategy. However frontline pressures make translating training into better care and better outcomes difficult. The aim of the paper is to highlight challenges in suicide risk assessment and management and introduce training frameworks to assist with mindful practice so professionals can strike a balance between risk and recovery. We combined the scientific literature with contemporary practice from two successful initiatives from Cambridgeshire, UK; 333 – a recovery oriented model of inpatient/community crisis care and PROMISE – a programme to reduce coercion in care by enhancing patient experience. The resulting PROTECT (PROactive deTECTion) frameworks operationalise ongoing practice of relational safety in these programmes. PROTECT is a combination of novel concepts and adaptations of well-established therapeutic approaches. It has four training frameworks: AWARE for reflection on clinical decisions; DESPAIR for assessment; ASPIRE for management; NOTES for documentation. PROTECT aims to improve self-awareness of mental shortcuts, risk taking thresholds and increase rigour through time efficient crosschecks. The training frameworks should support a relational approach to self-harm/suicide risk detection, mitigation and documentation, making care safer and person-centred. The goal is to enthuse practitioners with recovery oriented practice that draws on the strengths of the person in distress and their natural circle of support. It will provide the confidence to engage in participatory approaches to seek out unique individualised solutions to the overwhelming psychological pain of suicidal distress. Future collaborative research with people with lived and carer experience is needed for fine-tuning

High-Resolution Fibre Bragg Grating (FBG) Pressure Transducer for Low-Pressure Detection

Fibre Bragg grating (FBG) pressure sensor has shown great potential in replacing the conventional electrical pressure sensor due to its adaptability to the harsh environment. However, increasing its resolution for low-pressure measurement is still a challenge. In this work, the bonding of FBG to a rubber-based diaphragm pressure transducer was proposed. The proposed pressure transducer had enhanced the sensitivity to 117.7 pm/kPa across the range of 40 kPa, which corresponded to the pressure resolution of 0.008 kPa. Furthermore, the proposed pressure transducer possessed a reference FBG for temperature compensation, where the overall temperature effect was less than 1%

A Study Of Fiber Bragg Grating Temperature Sensor For Under Water Temperature Monitoring

Fiber Bragg grating (FBG) sensors have been widely utilized as a sensor for measuring strain, temperature, and vibration measurements. In this study, an optical FBG sensor system was developed to monitor the temperature fluctuation in water. The sensor was delicately packaged to eliminate the influence of strain acting on the sensor. The sensor had been submerged in iced water and the temperature was constantly increased by using an electric immersion heater. The experimental data were obtained to determine the temperature sensitivity of the FBG sensor. It is found that the relationship between the changes in temperature and changes in Bragg wavelength is virtually higher in linearity with R2 = 0.9997 and has superior sensitivity which is 10.13 pm/°C. This finding proves that the FBG sensor could be a good candidate for temperature-sensing device