452 research outputs found

    Sex chromosome dominance in a UV sexual system

    Get PDF
    Abstract: the alternation between multicellular haploid gametophytes and diploid sporophytes is a defining feature of most plant and algal life cycles. In such organisms, male and female sexes are determined in the haploid gametophyte with a female (U) or male (V) sex chromosome. Once the U and V chromosomes unite at fertilisation, sex determination no longer occurs, raising key questions about the fate of UV sex chromosomes in the diploid sporophyte stage of the life cycle. Here, we unravel the genetic and molecular interactions between the U and V chromosomes by assessing transcriptional and chromatin states across the life cycle of the brown algaEctocarpusalongsideouroborosmutants that decouple life cycle stage from ploidy. We reveal how sex chromosome genes are developmentally regulated across the life cycle, with genes involved in female sex determination in particular undergoing strong down-regulation in the sporophyte. Diploidouroborosmutants containing both a U and V sex chromosome behave as functional male gametophytes yet still exhibit feminized transcription, suggesting that presence of the V chromosome alone is insufficient to fully suppress female developmental program. Although the silencing of sex chromosome genes in the diploid sporophyte does not appear to correlate with localised changes in chromatin state, small RNAs may play a role in the repression of a female sex-linked gene. Finally, we show how histone H3K79me2 is globally re-configured in the diploid phase of the life cycle, including the sex determining region of the UV sex chromosomes. Contrary to its pattern in the haploid gametophyte, H3K79me2 no longer associates with repressed genes in the diploid sporophyte, suggesting that the function of this histone mark inEctocarpusmay be more complex than previously appreciated

    Interactions between U and V sex chromosomes during the life cycle of Ectocarpus

    Get PDF
    In many animals and flowering plants, sex determination occurs in the diploid phase of the life cycle with XX/XY or ZW/ZZ sex chromosomes. However, in early diverging plants and most macroalgae, sex is determined by female (U) or male (V) sex chromosomes in a haploid phase called the gametophyte. Once the U and V chromosomes unite at fertilization to produce a diploid sporophyte, sex determination no longer occurs, raising key questions about the fate of the U and V sex chromosomes in the sporophyte phase. Here, we investigate genetic and molecular interactions of the UV sex chromosomes in both the haploid and diploid phases of the brown alga Ectocarpus. We reveal extensive developmental regulation of sex chromosome genes across its life cycle and implicate the TALE-HD transcription factor OUROBOROS in suppressing sex determination in the diploid phase. Small RNAs may also play a role in the repression of a female sex-linked gene, and transition to the diploid sporophyte coincides with major reconfiguration of histone H3K79me2, suggesting a more intricate role for this histone mark in Ectocarpus development than previously appreciated.</p

    Interactions between U and V sex chromosomes during the life cycle of Ectocarpus

    Get PDF
    In many animals and flowering plants, sex determination occurs in the diploid phase of the life cycle with XX/XY or ZW/ZZ sex chromosomes. However, in early diverging plants and most macroalgae, sex is determined by female (U) or male (V) sex chromosomes in a haploid phase called the gametophyte. Once the U and V chromosomes unite at fertilization to produce a diploid sporophyte, sex determination no longer occurs, raising key questions about the fate of the U and V sex chromosomes in the sporophyte phase. Here, we investigate genetic and molecular interactions of the UV sex chromosomes in both the haploid and diploid phases of the brown alga Ectocarpus. We reveal extensive developmental regulation of sex chromosome genes across its life cycle and implicate the TALE-HD transcription factor OUROBOROS in suppressing sex determination in the diploid phase. Small RNAs may also play a role in the repression of a female sex-linked gene, and transition to the diploid sporophyte coincides with major reconfiguration of histone H3K79me2, suggesting a more intricate role for this histone mark in Ectocarpus development than previously appreciated.</p

    Survey of the ISM in Early-Type Galaxies. IV. The Hot Dust Component

    Get PDF
    We present mid-IR photometric properties for a sample of 28 early-type galaxies observed at 6.75, 9.63 and 15 um with the ISOCAM instrument on board the ISO satellite. We find total mid-IR luminosities in the range 3-48x10^8 L_sun. The spectral energy distribution (SED) of the galaxies were derived using the mid-IR data together with previously published UV, optical and near-IR data. These SEDs clearly show a mid-IR emission coming from dust heated at T ~ 260 K. Dust grains properties are inferred from the mid-IR colors. The masses of the hot dust component are in the range 10-400 M_sun. The relationship between the masses derived from mid-IR observations and those derived from visual extinction are discussed. The possible common heating source for the gas and dust is investigated through the correlations between Ha and mid-IR luminosities.Comment: 10 pages, LaTeX (aa.cls), 11 figures (f. 2-4 are colour plates). Accepted for publication in Astronomy & Astrophysic

    Repeated co-option of HMG-box genes for sex determination in brown algae and animals

    Get PDF
    In many eukaryotes, genetic sex determination is not governed by XX/XY or ZW/ZZ systems but by a specialized region on the poorly studied U (female) or V (male) sex chromosomes. Previous studies have hinted at the existence of a dominant male-sex factor on the V chromosome in brown algae, a group of multicellular eukaryotes distantly related to animals and plants. The nature of this factor has remained elusive. Here, we demonstrate that an HMG-box gene acts as the male-determining factor in brown algae, mirroring the role HMG-box genes play in sex determination in animals. Over a billion-year evolutionary timeline, these lineages have independently co-opted the HMG box for male determination, representing a paradigm for evolution’s ability to recurrently use the same genetic “toolkit” to accomplish similar tasks.</p

    Accumulation of α-synuclein mediates podocyte injury in Fabry nephropathy

    Get PDF
    Current therapies for Fabry disease are based on reversing intracellular accumulation of globotriaosylceramide (Gb3) by enzyme replacement therapy (ERT) or chaperone-mediated stabilization of the defective enzyme, thereby alleviating lysosomal dysfunction. However, their effect in the reversal of end-organ damage, like kidney injury and chronic kidney disease, remains unclear. In this study, ultrastructural analysis of serial human kidney biopsies showed that long-term use of ERT reduced Gb3 accumulation in podocytes but did not reverse podocyte injury. Then, a CRISPR/Cas9–mediated α-galactosidase knockout podocyte cell line confirmed ERT-mediated reversal of Gb3 accumulation without resolution of lysosomal dysfunction. Transcriptome-based connectivity mapping and SILAC-based quantitative proteomics identified α-synuclein (SNCA) accumulation as a key event mediating podocyte injury. Genetic and pharmacological inhibition of SNCA improved lysosomal structure and function in Fabry podocytes, exceeding the benefits of ERT. Together, this work reconceptualizes Fabry-associated cell injury beyond Gb3 accumulation, and introduces SNCA modulation as a potential intervention, especially for patients with Fabry nephropathy.publishedVersio

    Expansion-enhanced super-resolution radial fluctuations enable nanoscale molecular profiling of pathology specimens

    Get PDF
    Expansion microscopy physically enlarges biological specimens to achieve nanoscale resolution using diffraction-limited microscopy systems1. However, optimal performance is usually reached using laser-based systems (for example, confocal microscopy), restricting its broad applicability in clinical pathology, as most centres have access only to light-emitting diode (LED)-based widefield systems. As a possible alternative, a computational method for image resolution enhancement, namely, super-resolution radial fluctuations (SRRF)2,3, has recently been developed. However, this method has not been explored in pathology specimens to date, because on its own, it does not achieve sufficient resolution for routine clinical use. Here, we report expansion-enhanced super-resolution radial fluctuations (ExSRRF), a simple, robust, scalable and accessible workflow that provides a resolution of up to 25 nm using LED-based widefield microscopy. ExSRRF enables molecular profiling of subcellular structures from archival formalin-fixed paraffin-embedded tissues in complex clinical and experimental specimens, including ischaemic, degenerative, neoplastic, genetic and immune-mediated disorders. Furthermore, as examples of its potential application to experimental and clinical pathology, we show that ExSRRF can be used to identify and quantify classical features of endoplasmic reticulum stress in the murine ischaemic kidney and diagnostic ultrastructural features in human kidney biopsies.</p

    The AGN contribution to mid-infrared surveys

    Get PDF
    We provide constraints on the AGN contribution to the mid-IR extragalactic background light from a correlation analysis of deep X-ray and mid-IR observations in two regions centred on the Lockman Hole (LH) and Hubble Deep Field North (HDFN). Among the 76 galaxies detected by XMM in the LH area (200 square arcminutes), 24 show mid-IR emission, but the relative percentage of X-ray sources with mid-IR counterparts increases with the band energy: from 30% of the 0.5-2 keV sources up to 63% of the 5-10 keV sources. In contrast, only a small fraction of the mid-IR sources (around 10%) show X-ray emission. In the region centred on the HDFN (24 square arcminutes), 25% of the mid-IR sources are detected in the X-ray, while 30-40% of the X-ray sources show mid-IR emission. Under the conservative assumption that all XMM sources are AGN-dominated, AGNs contribute 15% of the total mid-IR flux in the LH. For the HDFN we have assumed that AGN-dominated sources are luminous X-ray sources with radio to X-ray SEDs typical of local AGNs, in which case we find that 18% of the mid-IR flux are due to AGN emission. If we put together all the existing information from the deepest HDFN data to the shallow large-area BeppoSAX observations in the ELAIS S1 region using the median mid-IR to X-ray spectral indices as a function of the X-ray flux, we find an AGN contribution to the 15um background of 17%. We conclude that the population of IR luminous galaxies detected in the ISOCAM deep surveys are mainly constituted by dust-obscured starbursts.Comment: 16 pages, 13 postscript + 3 jpg figures, A&A submitte
    • 

    corecore