Plant perception of β-aminobutyric acid is mediated by an aspartyl-tRNA synthetase

Specific chemicals can prime the plant immune system for augmented defense. β-aminobutyric acid (BABA) is a priming agent that provides broad-spectrum disease protection. However, BABA also suppresses plant growth when applied in high doses, which has hampered its application as a crop defense activator. Here we describe a mutant of Arabidopsis thaliana that is impaired in BABA-induced disease immunity (ibi1) but is hypersensitive to BABA-induced growth repression. IBI1 encodes an aspartyl-tRNA synthetase. Enantiomer-specific binding of the R enantiomer of BABA to IBI1 primed the protein for noncanonical defense signaling in the cytoplasm after pathogen attack. This priming was associated with aspartic acid accumulation and tRNA-induced phosphorylation of translation initiation factor eIF2α. However, mutation of eIF2α-phosphorylating GCN2 kinase did not affect BABA-induced immunity but relieved BABA-induced growth repression. Hence, BABA-activated IBI1 controls plant immunity and growth via separate pathways. Our results open new opportunities to separate broad-spectrum disease resistance from the associated costs on plant growth

Advanced image steganography based on exploiting modification direction and neutrosophic set

A new variant of image steganography based on exploiting modification directions (EMD) named advanced EMD (AEMD) is introduced. In this method the secret digits in mn -ary notional systems are embedded into a group of n pixels of the cover image. To increase data hiding capacity, edge masking characteristics of human visual system is exploited to embed more bits at image edge pixels than non-edge pixels. To do this, a neutrosophic set edge detector, named as MNSED is also introduced to classify cover image into 2 × 2 non-overlapping edge and non-edge blocks without any overhead information. Hence the secret digits in two different bases are embedded into the edge and non-edge blocks. Experimental results show that the proposed method provides higher embedding capacity and better quality compared to the existing schemes, while its resistance to steganographic attack is still higher