36 research outputs found
Phytolith Analysis for Differentiating between Foxtail Millet (Setaria italica) and Green Foxtail (Setaria viridis)
Foxtail millet (Setaria italica) is one of the oldest domesticated cereal crops in Eurasia, but identifying foxtail millets, especially in charred grains, and differentiating it from its wild ancestor, green foxtail (Setaria viridis), in the archaeobotanical remains, is still problematic. Phytolithic analysis provides a meaningful method for identifying this important crop. In this paper, the silicon structure patterns in the glumes, lemmas, and paleas from inflorescence bracts in 16 modern plants of foxtail millet and green foxtail from China and Europe are examined using light microscopy with phase-contrast and a microscopic interferometer. Our research shows that the silicon structure of ΩIII from upper lemmas and paleas in foxtail millet and green foxtail can be correspondingly divided into two groups. The size of ΩIII type phytolith of foxtail millet is bigger than that from green foxtail. Discriminant function analysis reveals that 78.4% of data on foxtail millet and 76.9% of data on green foxtail are correctly classified. This means certain morphotypes of phytoliths are relatively reliable tools for distinguishing foxtail millet from green foxtail. Our results also revealed that the husk phytolith morphologies of foxtail millets from China and Eastern Europe are markedly different from those from Western Europe. Our research gives a meaningful method of separating foxtail millet and green foxtail. The implications of these findings for understanding the history of foxtail millet domestication and cultivation in ancient civilizations are significant
Transcriptome profiling of ontogeny in the acridid grasshopper Chorthippus biguttulus
Acridid grasshoppers (Orthoptera:Acrididae) are widely used model organisms
for developmental, evolutionary, and neurobiological research. Although there
has been recent influx of orthopteran transcriptomic resources, many use
pooled ontogenetic stages obscuring information about changes in gene
expression during development. Here we developed a de novo transcriptome
spanning 7 stages in the life cycle of the acridid grasshopper Chorthippus
biguttulus. Samples from different stages encompassing embryonic development
through adults were used for transcriptomic profiling, revealing patterns of
differential gene expression that highlight processes in the different life
stages. These patterns were validated with semi-quantitative RT-PCR. Embryonic
development showed a strongly differentiated expression pattern compared to
all of the other stages and genes upregulated in this stage were involved in
signaling, cellular differentiation, and organ development. Our study is one
of the first to examine gene expression during post-embryonic development in a
hemimetabolous insect and we found that only the fourth and fifth instars had
clusters of genes upregulated during these stages. These genes are involved in
various processes ranging from synthesis of biogenic amines to chitin binding.
These observations indicate that post-embryonic ontogeny is not a continuous
process and that some instars are differentiated. Finally, genes upregulated
in the imago were generally involved in aging and immunity. Our study
highlights the importance of looking at ontogeny as a whole and indicates
promising directions for future research in orthopteran development
Evolution of resistance to scald, powdery mildew, and net blotch in barley composite cross II populations
Nuclear and cytoplasmic inheritance of resistance to infection by nodule bacteria in red clover
RESP-283