1,196 research outputs found
Experimental Ocular Toxoplasmosis in Genetically Susceptible and Resistant Mice
Genetic factors determining the pathogenesis and course of ocular toxoplasmosis are poorly understood. In this study, we explored the development of experimental ocular pathogenesis in genetically dissimilar mice infected with either the RH strain, the PLK strain, or the immunodominant surface antigen 1 (SAG1 [P30])-deficient mutant of the RH strain of Toxoplasma gondii. At 11 days postinfection, ocular infection of C57BL/6 mice with all of the strains of parasites resulted in severe inflammatory lesions and high numbers of parasites in eye tissue; less severe ocular lesions at earlier histopathology and prolonged survival were observed in this mouse strain infected with either the major surface antigen 1-deficient SAG1â/â strain or the less virulent PLK strain compared with RH infection. In contrast, both BALB/c and CBA/J mice had less severe lesions and low numbers of parasites in their eye tissue, and infection developed into the chronic stage in these mice. There were significantly higher serum levels of gamma interferon and tumor necrosis factor alpha in C57BL/6 mice than in BALB/c and CBA/J mice following ocular infection. These observations confirm earlier reports on systemic immunity to these parasites that the route of Toxoplasma infection markedly influences survival of mice. Our data indicate that genetic factors of the host as well as the parasite strain are critical in determining susceptibility to experimental ocular toxoplasmosis in murine models
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Common DNA sequence variation influences 3-dimensional conformation of the human genome.
BACKGROUND:The 3-dimensional (3D) conformation of chromatin inside the nucleus is integral to a variety of nuclear processes including transcriptional regulation, DNA replication, and DNA damage repair. Aberrations in 3D chromatin conformation have been implicated in developmental abnormalities and cancer. Despite the importance of 3D chromatin conformation to cellular function and human health, little is known about how 3D chromatin conformation varies in the human population, or whether DNA sequence variation between individuals influences 3D chromatin conformation. RESULTS:To address these questions, we perform Hi-C on lymphoblastoid cell lines from 20 individuals. We identify thousands of regions across the genome where 3D chromatin conformation varies between individuals and find that this variation is often accompanied by variation in gene expression, histone modifications, and transcription factor binding. Moreover, we find that DNA sequence variation influences several features of 3D chromatin conformation including loop strength, contact insulation, contact directionality, and density of local cis contacts. We map hundreds of quantitative trait loci associated with 3D chromatin features and find evidence that some of these same variants are associated at modest levels with other molecular phenotypes as well as complex disease risk. CONCLUSION:Our results demonstrate that common DNA sequence variants can influence 3D chromatin conformation, pointing to a more pervasive role for 3D chromatin conformation in human phenotypic variation than previously recognized
Plasma Depletion and Mirror Waves Ahead of Interplanetary Coronal Mass Ejections
We find that the sheath regions between fast interplanetary coronal mass
ejections (ICMEs) and their preceding shocks are often characterized by plasma
depletion and mirror wave structures, analogous to planetary magnetosheaths. A
case study of these signatures in the sheath of a magnetic cloud (MC) shows
that a plasma depletion layer (PDL) coincides with magnetic field draping
around the MC. In the same event, we observe an enhanced thermal anisotropy and
plasma beta as well as anti-correlated density and magnetic fluctuations which
are signatures of mirror mode waves. We perform a superposed epoch analysis of
ACE and Wind plasma and magnetic field data from different classes of ICMEs to
illuminate the general properties of these regions. For MCs preceded by shocks,
the sheaths have a PDL with an average duration of 6 hours (corresponding to a
spatial span of about 0.07 AU) and a proton temperature anisotropy -1.3, and are marginally unstable to the
mirror instability. For ICMEs with preceding shocks which are not MCs, plasma
depletion and mirror waves are also present but at a reduced level. ICMEs
without shocks are not associated with these features. The differences between
the three ICME categories imply that these features depend on the ICME geometry
and the extent of upstream solar wind compression by the ICMEs. We discuss the
implications of these features for a variety of crucial physical processes
including magnetic reconnection, formation of magnetic holes and energetic
particle modulation in the solar wind.Comment: fully refereed, accepted for publication in J. Geophys. Re
Regeneration of phase unlocked serial multiplexed DPSK signals in a single phase sensitive amplifier
All-optical WDM Regeneration of DPSK Signals using Optical Fourier Transformation and Phase Sensitive Amplification
We propose a novel all-optical WDM regeneration scheme for DPSK signals based on optical Fourier transformation and phase sensitive amplification. Phase regeneration of a WDM signal consisting of 4x10 Gbit/s phase noise degraded DPSK channels is demonstrated for the first time
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