Common promoter variant in cyclooxygenase-2 represses gene expression: evidence of role in acute-phase inflammatory response

Objective: Cyclooxygenase (COX)-2 is a key regulatory enzyme in the synthesis of prostanoids associated with trauma and inflammation. We investigated the COX-2 gene for functional variants that may influence susceptibility to disease. Methods and results: The promoter of COX-2 was screened for variants in healthy subjects by use of polymerase chain reaction-based methods. Promoter activity was investigated by using reporter expression experiments in human lung fibroblasts. Patients undergoing coronary artery bypass graft surgery, with measurements of plasma markers linked to COX-2 activity, were genotyped for association studies. A common COX-2 promoter variant, -765G>C, was found and shown to be carried by >25% of a group of healthy UK subjects. The -765C allele had significantly lower promoter activity compared with -765G, basally (28±3% lower, P<0.005) and in serum-stimulated cells (31±2% lower, P<0.005). In patients subjected to coronary artery bypass graft surgery, the magnitude of rise in levels of C-reactive protein (CRP) was strongly genotype dependent. Compared with -765G homozygotes, patients carrying the -765C allele had significantly lower plasma CRP levels at 1 to 4 days after surgery (14% lower at the peak of CRP levels on day 3, P<0.05 for all time points). Conclusions: For several acute and chronic inflammatory diseases, -765G>C may influence the variability of response observed

Insecticide ear tags numerically improve grazing cattle performance

Stocker cattle grazing pastures during the summer months face challenges due to horn flies, which can result in reduced weight gains and less efficient use of forages. One strategy for controlling horn flies is insecticide-impregnated ear tags. The use of pesticide ear tags may be an effective management practice to improve overall productivity during a grazing season. The objective of this study was to evaluate the efficacy of insecticide ear tags as a means of improving growth of stocker calves grazing native pastures in the Flint Hills region of Kansas

Study of Neutron-Induced Ionization in Helium and Argon Chamber Gases

Ion chambers used to monitor the secondary hadron and tertiary muon beam in the NuMI neutrino beamline will be exposed to background particles, including low energy neutrons produced in the beam dump. To understand these backgrounds, we have studied Helium- and Argon-filled ionization chambers exposed to intense neutron fluxes from PuBe neutron sources ($E_n=1-10$ MeV). The sources emit about 10$^8$ neutrons per second. The number of ion pairs in the chamber gas volume per incident neutron is derived. While limited in precision because of a large gamma ray background from the PuBe sources, our results are consistent with the expectation that the neutrons interact purely elastically in the chamber gas.Comment: accepted for publication in NIM

Innovations in Camera Trapping Technology and Approaches: The Integration of Citizen Science and Artificial Intelligence

Camera trapping has become an increasingly reliable and mainstream tool for surveying a diversity of wildlife species. Concurrent with this has been an increasing effort to involve the wider public in the research process, in an approach known as ‘citizen science’. To date, millions of people have contributed to research across a wide variety of disciplines as a result. Although their value for public engagement was recognised early on, camera traps were initially ill‐suited for citizen science. As camera trap technology has evolved, cameras have become more user‐friendly and the enormous quantities of data they now collect has led researchers to seek assistance in classifying footage. This has now made camera trap research a prime candidate for citizen science, as reflected by the large number of camera trap projects now integrating public participation. Researchers are also turning to Artificial Intelligence (AI) to assist with classification of footage. Although this rapidly‐advancing field is already proving a useful tool, accuracy is variable and AI does not provide the social and engagement benefits associated with citizen science approaches. We propose, as a solution, more efforts to combine citizen science with AI to improve classification accuracy and efficiency while maintaining public involvement

Recurrent rhabdomyolysis caused by carnitine palmitoyltransferase II deficiency, common but under-recognised: Lessons to be learnt

We discuss two adult siblings who presented with symptoms of myalgia and rhabdomyolysis following exercise with myoglobinuria; genetic testing confirmed carnitine palmitoyltransferase II deficiency and resulted in institution of appropriate crisis management and dietary advice. We explore the phenotypic variability of this commonest fatty oxidation defect that remains under-diagnosed in the adult population and provide clues for early recognition and diagnosis

Perfectionistic self-presentation and emotional experiences in music students: A three-wave longitudinal study

Research has shown that perfectionism predicts emotional experiences among amateur, professional and adolescent musicians. In examining these relationships, previous research has measured trait perfectionism and employed cross-sectional designs. The current study builds on existing research by examining whether perfectionistic self-presentation (as opposed to trait perfectionism) predicts negative and positive emotional experiences in music students over time. One hundred and forty-three music students (M age 18.92 years, SD = 2.96) enrolled in music-related degree programs completed measures of perfectionistic self-presentation (perfectionistic self-promotion, non-display of imperfection, and non-disclosure of imperfection) and emotional experiences (positive and negative feelings) at the start, middle and the end of the academic year. Path analysis revealed that perfectionistic self-promotion at the start of the year predicted lower positive feelings in the middle of the year, and non-disclosure of imperfection in the middle of the year predicted lower positive feelings at the end of the year. In addition, negative feelings in the middle of the year also predicted higher non-disclosure of imperfection at the end of the year. The findings suggest that the desire to present oneself perfectly and avoid disclosure of imperfections may contribute to less positive emotional experiences among music students

Assessing hazards and disaster risk on the coast for Pacific small island developing States: The need for a data-driven approach

Small island developing States, such as those in the Pacific, are often prone to multiple hazards that have potential to result in disaster and / or restrict development. Hazard data can be limited in resolution or omitted in or near SIDS’ coasts, but a growing and improved range of datasets are becoming available. Through an analysis of approximately 100 policy documents on hazards and disaster risk management in Pacific island nations, we found: limited information on hazards and how they manifest to disasters at local levels, thus not fully connecting driver and subsequent risk; at times a non-specific multi-hazard approach prompting needs to address more specific hazards; and restricted temporal and spatial scales of analysis that potentially limit continuity of actions where mitigation methods evolve. These limitations suggest that appropriate and timely high resolution hazard data, is needed from the top-down to underpin the design and development of local disaster risk management plans, simultaneous to local, bottom-up knowledge and interpretation to bring the realities of such hazard data to life. Developing and ensuring openly available hazard data will enable island States to develop more robust, inclusive disaster risk management plans and mitigation policies, plus aid inter-island comparison for communal learning

Organic Pollutants, Heavy Metals and Toxicity in Oil Spill impacted Salt Marsh Sediment Cores, Staten Island, New York City, USA

Sediment cores from Staten Island's salt marsh contain multiple historical oil spill events that impact ecological health. Microtox solid phase bioassay indicated moderate to high toxicity. Multiple spikes of TPH (6524 to 9586 mg/kg) and Σ16 PAH (15.5 to 18.9 mg/kg) were co-incident with known oil spills. A high TPH background of 400–700 mg/kg was attributed to diffuse sources. Depth-profiled metals Cu (1243 mg/kg), Zn (1814 mg/kg), Pb (1140 mg/kg), Ni (109 mg/kg), Hg (7 mg/kg), Cd 15 (mg/kg) exceeded sediment quality guidelines confirming adverse biological effects. Changes in Pb206/207 suggested three metal contaminant sources and diatom assemblages responded to two contamination events. Organic and metal contamination in Saw Mill Creek Marsh may harm sensitive biota, we recommend caution in the management of the 20–50 cm sediment interval because disturbance could lead to remobilisation of pre-existing legacy contamination into the waterway