Development of a Practical Kit and a Citizen Scientist Program for Learning Chemistry

Laboratory practical is an essential component for effective learning of science. Providing laboratory experience can be challenging for some rural schools with limited laboratory facilities. The situation was exacerbated when schools were closed due to the COVID-19 pandemic. This thesis reports a laboratory kit design based on the Standard Curriculum for Secondary Schools in Malaysia (Kurikulum Standard Sekolah Menengah, KSSM). The kit was evaluated by students and experienced teachers from town and rural schools. Pre- and post-test was conducted with the students before and after using the kit. A questionnaire was disseminated to appraise the students’ perceptions on practical learning. There was a significant improvement (p < 0.05) in the overall score of the post-test (90.32) compared to the pre-test (71.00). Both rural and town schools demonstrated a significant increase in the overall mean scores, from 68.67 and 73.34 in the pre-test to 91.34 and 89.33 in the post-test. There was also improvement in affection for chemistry among students after using the kit. The practical kit can be deployed in schools with laboratory constraints and to be used outside the school settings. It was designed to be affordable compared to traditional lab setting, safe and environmental-friendly due to minimal chemical used, and providing individualized hands-on exposure. Besides, this thesis also reports a citizen science program on household food waste for students to introduce the idea of citizen science to them and to evaluate their learning experience. The project focused on quantification of avoidable solid food wastes in the households using the food waste diary. The results were positive, that is more than 80% of the participants agreed that this program increased their interest in chemistry learning, raised awareness of food waste reduction and waste management, and improved scientific skills such as food waste measurement

A curriculum-based laboratory kit for flexible teaching and learning of practical chemistry

Laboratory practical is an essential component for effective learning of science. To provide laboratory experience can be a challenge to some rural schools with limited laboratory facilities. The situation was exacerbated when schools were close due to the COVID pandemic. This paper reports a laboratory kit designed based on the Standard Curriculum for Secondary Schools in Malaysia (KSSM). The kit was evaluated by students and experienced teachers from town and rural schools. Pre- and post-test was conducted with the students before and after using the kit. A questionnaire was disseminated to appraise the students’ perceptions on practical learning. There was a significant improvement (p < 0.05) in the overall score of the post-test comparing to the pre-test. Both rural and town schools demonstrated a marked increase in the overall mean scores in the post-test. There was also improvement in affection for chemistry among students after using the kit. The practical kit can be deployed in schools with laboratory constraints and to be used outside the school settings. It was designed to be affordable, safe and environmental-friendly, providing individualized hands-on exposure

THE LEVEL OF READINES TRAINEE TEACHERS IN THE INSTITUTE OF TEACHER EDUCATION TECHNICAL EDUCATION CAMPUS FOR SPECIAL NEEDS STUDENTS IN TVET SUBJECTS

In Malaysia, studies on students with special needs in technical and vocational education are lacking. Therefore, this study aims to identify the level of readiness of trainee teachers towards the implementation of the teaching and learning process for technical and vocational subjects namely Design and Technology. This study uses a survey method using a 4-point Likert scale questionnaire as a research instrument involving a sample of 154 teacher trainees at the Technical Education Campus Teacher Education Institution who took the Design and Technology (RBT) course. The size of the selected respondents was by simple random sampling using Krejcie & Morgan's Sample Size Determination Table. Descriptive analysis was used to obtain the mean value, standard deviation, frequency, and percentage to see the level of preparedness of trainee teachers towards Special Needs Students (SNS) in TVET subjects. The findings of the study show the level of knowledge and skills of trainee teachers regarding the implementation of teaching and learning of Design and Technology subjects for students with Special Needs (SNS) which is overall at a moderate level. The results of the analysis found that the level of knowledge and skills of trainee teachers at Teacher Education Institutions concerning the implementation of the teaching and learning of Design and Technology subjects for Students with Special Needs (SNS) needs to be improved. This study is expected to provide input on the level of preparedness of trainee teachers at the Technical Education Campus Teacher Education Institution in teaching students with special educational needs in TVET subjects. Improving the knowledge and skills of Design and Technology trainee teachers at the Technical Education Campus Teacher Education Institution will have a positive impact on the effectiveness of the teaching and learning delivered by the trainee teachers, which in turn can improve the quality of learning for Special Needs Students (SNS) in the classroom. In conclusion, every trainee teacher needs to be given sufficient training and education to help Special Needs Students (SNS) in Design and Technology subjects

Evaluating the coverage and potential of imputing the exome microarray with next-generation imputation using the 1000 genomes project

10.1371/journal.pone.0106681PLoS ONE991-

A review of clinical and surgical outcomes of endoscopic endonasal skull base surgery in a Tertiary Center in Sarawak

Background: Endoscopic endonasal approach (EEA) for skull base tumor has been extensively developed in recent years. Objectives: To review the demographic data, indications, clinical presentations and surgical outcomes of endoscopic endonasal skull base surgeries performed. Methods: A retrospective analysis on all patients who had undergone endoscopic endonasal skull base surgery at the Skull Base Unit (ENT & Neurosurgery) from December 2013 to December 2015. Results: A total 34 cases were operated on during the study period. Female patients account for 44% of patients while 56% were male patients, with ages ranging from 8 to 77 years, with the mean age of 51.88 years. Majority of the cases were pituitary tumors (41%), followed by sinonasal tumors (15%), meningioma (12%), clival tumor (8%), cerebrospinal fluid (CSF) leak repair (8%) and frontal mucocoele (6%). Transsellar approach was the commonest approach (41%) followed by the transclival, transplanum, transfrontal approaches. In about 80% of cases, CSF leak was encountered intraoperatively and was successfully repaired endoscopically with the Hadad-Bassagasteguy flap and with the insertion of a lumbar drain. Only one case (3%) of CSF leak was noted postoperatively which was then successful repaired endoscopically with a nasoseptal flap. Systemic complications and intracranial infections were seen in 3 cases (8%) and were managed accordingly. No cases of epitaxis requiring surgical intervention were noted post operatively

A study assessing the association of glycated hemoglobin a1C (HbA1C) associated variants with HbA1C, chronic kidney disease and diabetic retinopathy in populations of asian ancestry

10.1371/journal.pone.0079767PLoS ONE811-POLN

Are C-Reactive Protein Associated Genetic Variants Associated with Serum Levels and Retinal Markers of Microvascular Pathology in Asian Populations from Singapore?

Introduction:C-reactive protein (CRP) levels are associated with cardiovascular disease and systemic inflammation. We assessed whether CRP-associated loci were associated with serum CRP and retinal markers of microvascular disease, in Asian populations.Methods:Genome-wide association analysis (GWAS) for serum CRP was performed in East-Asian Chinese (N = 2,434) and Malays (N = 2,542) and South-Asian Indians (N = 2,538) from Singapore. Leveraging on GWAS data, we assessed, in silico, association levels among the Singaporean datasets for 22 recently identified CRP-associated loci. At loci where directional inconsistencies were observed, quantification of inter-ethnic linkage disequilibrium (LD) difference was determined. Next, we assessed association for a variant at CRP and retinal vessel traits [central retinal artery equivalent (CRAE) and central retinal vein equivalent (CRVE)] in a total of 24,132 subjects of East-Asian, South-Asian and European ancestry.Results:Serum CRP was associated with SNPs in/near APOE, CRP, HNF1A and LEPR (p-values ≤4.7×10-8) after meta-analysis of Singaporean populations. Using a candidate-SNP approach, we further replicated SNPs at 4 additional loci that had been recently identified to be associated with serum CRP (IL6R, GCKR, IL6 and IL1F10) (p-values ≤0.009), in the Singaporean datasets. SNPs from these 8 loci explained 4.05% of variance in serum CRP. Two SNPs (rs2847281 and rs6901250) were detected to be significant (p-value ≤0.036) but with opposite effect directions in the Singaporean populations as compared to original European studies. At these loci we did not detect significant inter-population LD differences. We further did not observe a significant association between CRP variant and CRVE or CRAE levels after meta-analysis of all Singaporean and European datasets (p-value >0.058).Conclusions:Common variants associated with serum CRP, first detected in primarily European studies, are also associated with CRP levels in East-Asian and South-Asian populations. We did not find a causal link between CRP and retinal measures of microvascular disease

A statistical method for region-based meta-analysis of genome-wide association studies in genetically diverse populations

Genome-wide association studies (GWAS) have become the preferred experimental design in exploring the genetic etiology of complex human traits and diseases. Standard SNP-based meta-analytic approaches have been utilized to integrate the results from multiple experiments. This fundamentally assumes that the patterns of linkage disequilibrium (LD) between the underlying causal variants and the directly genotyped SNPs are similar across the populations for the same SNPs to emerge with surrogate evidence of disease association. We introduce a novel strategy for assessing regional evidence of phenotypic association that explicitly incorporates the extent of LD in the region. This provides a natural framework for combining evidence from multi-ethnic studies of both dichotomous and quantitative traits that (i) accommodates different patterns of LD, (ii) integrates different genotyping platforms and (iii) allows for the presence of allelic heterogeneity between the populations. Our method can also be generalized to perform gene-based or pathway-based analyses. Applying this method on real GWAS data in type 2 diabetes (T2D) boosted the association evidence in regions well-established for T2D etiology in three diverse South-East Asian populations, as well as identified two novel gene regions and a biologically convincing pathway that are subsequently validated with data from the Wellcome Trust Case Control Consortium

Genome-Wide Meta-Analysis of Five Asian Cohorts Identifies PDGFRA as a Susceptibility Locus for Corneal Astigmatism

Corneal astigmatism refers to refractive abnormalities and irregularities in the curvature of the cornea, and this interferes with light being accurately focused at a single point in the eye. This ametropic condition is highly prevalent, influences visual acuity, and is a highly heritable trait. There is currently a paucity of research in the genetic etiology of corneal astigmatism. Here we report the results from five genome-wide association studies of corneal astigmatism across three Asian populations, with an initial discovery set of 4,254 Chinese and Malay individuals consisting of 2,249 cases and 2,005 controls. Replication was obtained from three surveys comprising of 2,139 Indians, an additional 929 Chinese children, and an independent 397 Chinese family trios. Variants in PDGFRA on chromosome 4q12 (lead SNP: rs7677751, allelic odds ratio = 1.26 (95% CI: 1.16–1.36), Pmeta = 7.87×10−9) were identified to be significantly associated with corneal astigmatism, exhibiting consistent effect sizes across all five cohorts. This highlights the potential role of variants in PDGFRA in the genetic etiology of corneal astigmatism across diverse Asian populations

Transferability of Type 2 Diabetes Implicated Loci in Multi-Ethnic Cohorts from Southeast Asia

Recent large genome-wide association studies (GWAS) have identified multiple loci which harbor genetic variants associated with type 2 diabetes mellitus (T2D), many of which encode proteins not previously suspected to be involved in the pathogenesis of T2D. Most GWAS for T2D have focused on populations of European descent, and GWAS conducted in other populations with different ancestry offer a unique opportunity to study the genetic architecture of T2D. We performed genome-wide association scans for T2D in 3,955 Chinese (2,010 cases, 1,945 controls), 2,034 Malays (794 cases, 1,240 controls), and 2,146 Asian Indians (977 cases, 1,169 controls). In addition to the search for novel variants implicated in T2D, these multi-ethnic cohorts serve to assess the transferability and relevance of the previous findings from European descent populations in the three major ethnic populations of Asia, comprising half of the world's population. Of the SNPs associated with T2D in previous GWAS, only variants at CDKAL1 and HHEX/IDE/KIF11 showed the strongest association with T2D in the meta-analysis including all three ethnic groups. However, consistent direction of effect was observed for many of the other SNPs in our study and in those carried out in European populations. Close examination of the associations at both the CDKAL1 and HHEX/IDE/KIF11 loci provided some evidence of locus and allelic heterogeneity in relation to the associations with T2D. We also detected variation in linkage disequilibrium between populations for most of these loci that have been previously identified. These factors, combined with limited statistical power, may contribute to the failure to detect associations across populations of diverse ethnicity. These findings highlight the value of surveying across diverse racial/ethnic groups towards the fine-mapping efforts for the casual variants and also of the search for variants, which may be population-specific