Up-regulation of MiR-205 under hypoxia promotes epithelial-mesenchymal transition by targeting ASPP2

The epithelial–mesenchymal transition (EMT) is one of the crucial procedures for cancer invasion and distal metastasis. Despite undergoing intensive studies, the mechanisms underlying EMT remain to be completely elucidated. Here, we identified that apoptosis-stimulating protein of p53-2 (ASPP2) is a novel target of MiR-205 in various cancers. Interestingly, the binding site of MiR-205 at the 3′-untranslated region of ASPP2 was highly conserved among different species. An inverse correlation between MiR-205 and ASPP2 was further observed in vivo in cervical cancers, suggesting MiR-205 may be an important physiological inhibitor of ASPP2. Hypoxia is a hallmark of solid tumor microenvironment and one of such conditions to induce EMT. Notably, MiR-205 was remarkably induced by hypoxia in cervical and lung cancer cells. A marked suppression of ASPP2 was observed simultaneously. Further studies confirmed that hypoxia-induced ASPP2 suppression was mainly attributed to the elevated MiR-205. Interestingly, the alteration of MiR-205/ASPP2 under hypoxia was accompanied with the decreased epithelial marker E-cadherin and increased mesenchymal marker Vimentin, as well as a morphological transition from the typical cobblestone-like appearance to the mesenchymal-like structure. More importantly, MiR-205 mimics or ASPP2 silencing similarly promoted EMT process. By contrast, ASPP2 recovery or MiR-205 inhibitor reversed MiR-205-dependent EMT. Further studies demonstrated that the newly revealed MiR-205/ASPP2 axis promoted cell migration and also increased cell proliferation both in vivo and in vitro. These data together implicated a critical impact of MiR-205/ASPP2 on promoting EMT. MiR-205/ASPP2 may be potential diagnostic and therapeutic biomarkers in cervical and lung cancers

A novel dilated contextual attention module for breast cancer mitosis cell detection

Background and object: Mitotic count (MC) is a critical histological parameter for accurately assessing the degree of invasiveness in breast cancer, holding significant clinical value for cancer treatment and prognosis. However, accurately identifying mitotic cells poses a challenge due to their morphological and size diversity.Objective: We propose a novel end-to-end deep-learning method for identifying mitotic cells in breast cancer pathological images, with the aim of enhancing the performance of recognizing mitotic cells.Methods: We introduced the Dilated Cascading Network (DilCasNet) composed of detection and classification stages. To enhance the model’s ability to capture distant feature dependencies in mitotic cells, we devised a novel Dilated Contextual Attention Module (DiCoA) that utilizes sparse global attention during the detection. For reclassifying mitotic cell areas localized in the detection stage, we integrate the EfficientNet-B7 and VGG16 pre-trained models (InPreMo) in the classification step.Results: Based on the canine mammary carcinoma (CMC) mitosis dataset, DilCasNet demonstrates superior overall performance compared to the benchmark model. The specific metrics of the model’s performance are as follows: F1 score of 82.9%, Precision of 82.6%, and Recall of 83.2%. With the incorporation of the DiCoA attention module, the model exhibited an improvement of over 3.5% in the F1 during the detection stage.Conclusion: The DilCasNet achieved a favorable detection performance of mitotic cells in breast cancer and provides a solution for detecting mitotic cells in pathological images of other cancers

An Extended Fourier Approach to Improve the Retrieved Leaf Area Index (LAI) in a Time Series from an Alpine Wetland

An extended Fourier approach was presented to improve the retrieved leaf area index (LAIr) of herbaceous vegetation in a time series from an alpine wetland. The retrieval was performed from the Aqua MODIS 8-day composite surface reflectance product (MYD09Q1) from day of year (DOY) 97 to 297 using a look-up table (LUT) based inversion of a two-layer canopy reflectance model (ACRM). To reduce the uncertainty (the ACRM inversion is ill-posed), we used NDVI and NIR images to reduce the influence of the soil background and the priori information to constrain the range of sensitive ACRM parameters determined using the Sobol’s method. Even so the uncertainty caused the LAIr versus time curve to oscillate. To further reduce the uncertainty, a Fourier model was fitted using the periodically LAIr results, obtaining LAIF. We note that the level of precision of the LAIF potentially may increase through removing singular points or decrease if the LAIr data were too noisy. To further improve the precision level of the LAIr, the Fourier model was extended by considering the LAIr uncertainty. The LAIr, the LAI simulated using the Fourier model, and the LAI simulated using the extended Fourier approach (LAIeF) were validated through comparisons with the field measured LAI. The R2 values were 0.68, 0.67 and 0.72, the residual sums of squares (RSS) were 3.47, 3.42 and 3.15, and the root-mean-square errors (RMSE) were 0.31, 0.30 and 0.29, respectively, on DOY 177 (early July 2011). In late August (DOY 233), the R2 values were 0.73, 0.77 and 0.79, the RSS values were 38.96, 29.25 and 27.48, and the RMSE values were 0.94, 0.81 and 0.78, respectively. The results OPEN ACCESS Remote Sens. 2014, 6 1172 demonstrate that the extended Fourier approach has the potential to increase the level of precision of estimates of the time varying LAI

Globe-LFMC 2.0, an enhanced and updated dataset for live fuel moisture content research

Globe-LFMC 2.0, an updated version of Globe-LFMC, is a comprehensive dataset of over 280,000 Live Fuel Moisture Content (LFMC) measurements. These measurements were gathered through field campaigns conducted in 15 countries spanning 47 years. In contrast to its prior version, Globe-LFMC 2.0 incorporates over 120,000 additional data entries, introduces more than 800 new sampling sites, and comprises LFMC values obtained from samples collected until the calendar year 2023. Each entry within the dataset provides essential information, including date, geographical coordinates, plant species, functional type, and, where available, topographical details. Moreover, the dataset encompasses insights into the sampling and weighing procedures, as well as information about land cover type and meteorological conditions at the time and location of each sampling event. Globe-LFMC 2.0 can facilitate advanced LFMC research, supporting studies on wildfire behaviour, physiological traits, ecological dynamics, and land surface modelling, whether remote sensing-based or otherwise. This dataset represents a valuable resource for researchers exploring the diverse LFMC aspects, contributing to the broader field of environmental and ecological research

Uniaxial tension-compression fatigue behavior and fiber bridging degradation of strain hardening fiber reinforced cementitious composites

This study aims to clarify experimentally the uniaxial tension-compression fatigue behavior and fiber bridging stress degradation of strain hardening fiber reinforced cementitious composites. During fatigue cyclic loading, tensile bridging stress corresponding to the preset maximum tensile strain was recorded with the number of cycles in order to observe the degradation of the tensile bridging stress. Major bridging stress degradation was observed before reaching 1,000 cycles, and microscopic observation of the failure surface confirmed severe fiber damages. Fitting curves of bridging stress degradation are proposed with the idea of Weibull distribution. The preliminary results of the current study with a limited number of specimens are compared with those of a uniaxial tensile fatigue study, and the differences of bridging stress degradations are discussed

SEISMIC RESPONSE OF CURVED GRILLAGE GIRDER VIADUCTS WITH BASE ISOLATION SYSTEM IN COLD REGION

The Thirteenth East Asia-Pacific Conference on Structural Engineering and Construction (EASEC-13), September 11-13, 2013, Sapporo, Japan

A STUDY ON DYNAMIC RESPONSE OF A CURVED VIADUCT SYSTEM WITH INTEGRATED SLIDING BEARING IN CONSIDERATION OF THE DIRECTION OF EARTHQUAKE

The Thirteenth East Asia-Pacific Conference on Structural Engineering and Construction (EASEC-13), September 11-13, 2013, Sapporo, Japan

Effects of Live Fuel Moisture Content on Wildfire Occurrence in Fire-Prone Regions over Southwest China

Previous studies have shown that Live Fuel Moisture Content (LFMC) is a crucial driver affecting wildfire occurrence worldwide, but the effect of LFMC in driving wildfire occurrence still remains unexplored over the southwest China ecosystem, an area historically vulnerable to wildfires. To this end, we took 10-years of LFMC dynamics retrieved from Moderate Resolution Imaging Spectrometer (MODIS) reflectance product using the physical Radiative Transfer Model (RTM) and the wildfire events extracted from the MODIS Burned Area (BA) product to explore the relations between LFMC and forest/grassland fire occurrence across the subtropical highland zone (Cwa) and humid subtropical zone (Cwb) over southwest China. The statistical results of pre-fire LFMC and cumulative burned area show that distinct pre-fire LFMC critical thresholds were identified for Cwa (151.3%, 123.1%, and 51.4% for forest, and 138.1%, 72.8%, and 13.1% for grassland) and Cwb (115.0% and 54.4% for forest, and 137.5%, 69.0%, and 10.6% for grassland) zones. Below these thresholds, the fire occurrence and the burned area increased significantly. Additionally, a significant decreasing trend on LFMC dynamics was found during the days prior to two large fire events, Qiubei forest fire and Lantern Mountain grassland fire that broke during the 2009/2010 and 2015/2016 fire seasons, respectively. The minimum LFMC values reached prior to the fires (49.8% and 17.3%) were close to the lowest critical LFMC thresholds we reported for forest (51.4%) and grassland (13.1%). Further LFMC trend analysis revealed that the regional median LFMC dynamics for the 2009/2010 and 2015/2016 fire seasons were also significantly lower than the 10-year LFMC of the region. Hence, this study demonstrated that the LFMC dynamics explained wildfire occurrence in these fire-prone regions over southwest China, allowing the possibility to develop a new operational wildfire danger forecasting model over this area by considering the satellite-derived LFMC product

Model-driven estimation of closed and open shrublands live fuel moisture content

Live fuel moisture content (LFMC) is a crucial variable affecting the ignition potential of shrublands. Different remote sensing-based models (either empirical or physical) have been adopted to estimate LFMC in shrublands but with mixed success potentially owing to differences in vegetation cover (closed vs. open shrublands). This study aimed to evaluate and discuss LFMC estimation in open and closed shrublands using different remote sensing approaches. For each case, three broadly used radiative transfer models (RTMs) (PROSAILH, PROGeoSail, and PROACRM), and two empirical models were selected and compared. The empirical models were calibrated by a stepwise regression approach using a spectral index (SI) and its normalized form (SImax-min). Results showed that both RTMs and empirical models performed well in retrieving LFMC of closed shrublands (RTMs: R2 = 0.60–0.66, RMSE = 14.96–18.51%, bias = −5.99–4.36%, and empirical models: R2 = 0.69–0.72, RMSE = 10.67–11.30%, bias = 0.18–0.35%). However, all RTMs failed to retrieve LFMC for open shrublands (R2 = 0.01–0.09, RMSE = 45.21–48.66%, bias = 9.76–14.75%) potentially due to the high heterogeneity of vegetation in this vegetation type. In contrast, the SImax-min-based model outperformed the RTMs for the open shrublands LFMC estimation (R2 = 0.44, RMSE = 32.32%, bias = −0.34%) but saturated at high LFMC values (> 120%). In conclusion, PROACRM and the empirical model using SImax-min as an explanatory variable are recommended to model closed and open shrublands LFMC, respectively. This study gives insights into developing effective models for improving shrubland LFMC estimation by considering various fractions of covers of shrublands that were not previously considered