How to Track Protists in Three Dimensions

We present an apparatus optimized for tracking swimming microorganisms in the size range 10-1000 microns, in three dimensions (3D), far from surfaces, and with negligible background convective fluid motion. CCD cameras attached to two long working distance microscopes synchronously image the sample from two perpendicular directions, with narrowband dark-field or bright-field illumination chosen to avoid triggering a phototactic response. The images from the two cameras can be combined to yield 3D tracks of the organism. Using additional, highly directional broad-spectrum illumination with millisecond timing control the phototactic trajectories in 3D of organisms ranging from Chlamydomonas to Volvox can be studied in detail. Surface-mediated hydrodynamic interactions can also be investigated without convective interference. Minimal modifications to the apparatus allow for studies of chemotaxis and other taxes.Comment: 8 pages, 7 figure

How brain asymmetry relates to performance – a large scale dichotic listening study

All major mental functions including language, spatial and emotional processing are lateralized but how strongly and to which hemisphere is subject to inter- and intraindividual variation. Relatively little, however, is known about how the degree and direction of lateralization affect how well the functions are carried out, i.e., how lateralization and task performance are related. The present study therefore examined the relationship between lateralization and performance in a dichotic listening task for which we had data available from 1839 participants. In this task, consonant-vowel syllables are presented simultaneously to the left and right ear, such that each ear receives a different syllable. When asked which of the two they heard best, participants typically report more syllables from the right ear, which is a marker of left-hemispheric speech dominance. We calculated the degree of lateralization (based on the difference between correct left and right ear reports) and correlated it with overall response accuracy (left plus right ear reports). In addition, we used reference models to control for statistical interdependency between left and right ear reports. The results revealed a u-shaped relationship between degree of lateralization and overall accuracy: the stronger the left or right ear advantage, the better the overall accuracy. This u-shaped asymmetry-performance relationship consistently emerged in males, females, right-/non-right-handers, and different age groups. Taken together, the present study demonstrates that performance on lateralized language functions depends on how strongly these functions are lateralized. The present study further stresses the importance of controlling for statistical interdependency when examining asymmetry-performance relationships in general

Bosonic effective action for interacting fermions

We compare different versions of a bosonic description for systems of interacting fermions, with particular emphasis on the free energy functional. The bosonic effective action makes the issue of symmetries particularly transparent and we present for the Hubbard model an exact mapping between repulsive and attractive interactions. A systematic expansion for the bosonic effective action starts with a solution to the lowest order Schwinger-Dyson or gap equation. We propose a two particle irreducible formulation of an exact functional renormalization group equation for computations beyond leading order. On this basis we suggest a renormalized gap equation. This approach is compared with functional renormalization in a partially bosonized setting.Comment: new sections on exact mapping between attractive and repulsive Hubbard model and relation between two-particle-irreducible formalism, 32 pages,1 figure,LaTe

The Role of Bacteria and Pattern Recognition Receptors in GvHD

Graft-versus-Host Disease (GvHD) is the most serious complication of allogeneic stem cell transplantation (SCT) and results from an activation of donor lymphocytes by recipient antigen-presenting cells (APCs). For a long time, it has been postulated that the intestinal microflora and endotoxin exert a crucial step in this APC activation, as there is early and severe gastrointestinal damage induced by pretransplant conditioning. With the detailed description of pathogen-associated molecular patterns and pathogen recognition receptors single nucleotide polymorphisms of TLRs and especially NOD2 have been identified as potential risk factors of GvHD and transplant related complications thus further supporting the crucial role of innate immunity in SCT, related complications. Gastrointestinal decontamination and neutralization of endotoxin have been used to interfere with this early axis of activation with some success but more specific approaches of modulation of innate immunity are needed for further improvement of clinical outcome

High-resolution 3D phenotyping of the grapevine root system using X-ray Computed Tomography

Plant roots are essential for water and nutrient uptake and contribute to the plants' response to environmental stress factors. As the hidden half of a plant, investigation of root systems is highly challenging, most of available methods are destructive and very labour-intensive. In this proof-of-concept study, a non-invasive X-ray micro computed tomography (X-ray µCT) method was applied to investigate the phenotypic variation of the complex three-dimensional (3D) architecture of grapevine roots as a function of genotype and soil. Woody cuttings of 'Calardis Musqué', 'Villard Blanc' and V3125 ('Schiava Grossa' x 'Riesling') were cultivated in polypropylene columns filled with two different soil types, clay loam and sandy loam, for 6 weeks. Afterwards, the columns were scanned once using the technique of X-ray µCT. The received raw data were analysed for the reconstruction of 3D root system models (3D model), which display a non-destructive visualization of whole, intact root systems with a spatial resolution of 42 µm. The 3D models of all investigated plants (in total 18) were applied to quantify root system characteristics precisely by measuring adventitious root length, lateral root length, total root length, root system surface area, root system volume and root growth angles from the woody cutting relative to a horizontal axis. The results showed that: (i) early root formation and root growth differed between genotypes, especially between 'Calardis Musqué' and 'Villard Blanc'; and (ii) the soil type does influence adventitious root formation of V3125, but had minor effects on 'Calardis Musqué' and 'Villard Blanc'. In conclusion, this innovative, high-resolution method of X-ray µCT is suitable for high resolution phenotyping of root formation, architecture, and rooting characteristics of grapevine woody cuttings in a non-destructive manner, e.g. to investigate root response to drought stress and would provide new insights into phylloxera root infection