Analysis of Heart Rate Variability in Male and Female Rats

poster abstractHeart disease is the leading cause of death in the United States. Quantitative measures of cardiovascular function are often essential to effective clinical interventions. The QRS complex is one such measure widely used by cardiologists. These analyses can involve subtle changes in the magnitude and time course of the QRS complex, to differences in the timing between successive heart beats. Electrocardiograms (ECG) are continuous recordings of the QRS complex at various locations across the body surface and provide a comprehensive measurement of the electrical activity of the heart. Knowledge obtained from investigating ECG signal characteristics can help the cardiologist diagnose possible health or cardiac abnormalities such as arrhythmias and can provide objective measures of heart health following injury such as myocardial infarction. Heart rate variability (HRV) can also serve as a reliable indicator of heart health and has been shown to be a strong indicator of mortality and morbidity following myocardial infarction. Unfortunately, very little is known concerning the neurophysiological mechanisms underlying HRV beyond the broader impact of the autonomic nervous system and associated neurocirculatory reflexes. In this research project, we first implemented several established methods for quantifying HRV in male and female rats such as calculating the power spectral density of a long time series of HRV measures, and calculating the standard deviation of the averages of all beat-to-beat intervals in the recording. These measures compared well to those in the literature supporting the accuracy and reliability of the Matlab scripts created to process these data. Simultaneous recordings of the QRS complex and femoral arterial pressure (BP) provided the opportunity to determine how well BP recordings could be used to quantify HRV. In addition, HRV measurements were compared across populations of male and normal, cycling (OVI) and ovariectomized (OVX) female rats in order to determine if HRV is sexually dimorphic. Mentors: John H. Schild, Grace Santa Cruz Chavez, Department of Biomedical Engineering, Purdue School of Engineering and Technology, IUPUI, Indianapolis, I

Molecular pathway reconstruction and analysis of disturbed gene expression in depressed individuals who died by suicide

Molecular mechanisms behind the etiology and pathophysiology of major depressive disorder and suicide remain largely unknown. Recent molecular studies of expression of serotonin, GABA and CRH receptors in various brain regions have demonstrated that molecular factors may contribute to the development of depressive disorder and suicide behaviour. Here, we used microarray analysis to examine the expression of genes in brain tissue (frontopolar cortex) of individuals who had been diagnosed with major depressive disorder and died by suicide, and those who had died suddenly without a history of depression. We analyzed the list of differentially expressed genes using pathway analysis, which is an assumption-free approach to analyze microarray data. Our analysis revealed that the differentially expressed genes formed functional networks that were implicated in cell to cell signaling related to synapse maturation, neuronal growth and neuronal complexity. We further validated these data by randomly choosing (100 times) similarly sized gene lists and subjecting these lists to the same analyses. Random gene lists did not provide highly connected gene networks like those generated by the differentially expressed list derived from our samples. We also found through correlational analysis that the gene expression of control participants was more highly coordinated than in the MDD/suicide group. These data suggest that among depressed individuals who died by suicide, wide ranging perturbations of gene expression exist that are critical for normal synaptic connectively, morphology and cell to cell communication

Null Strings in Schwarzschild Spacetime

The null string equations of motion and constraints in the Schwarzschild spacetime are given. The solutions are those of the null geodesics of General Relativity appended by a null string constraint in which the "constants of motion" depend on the world-sheet spatial coordinate. Because of the extended nature of a string, the physical interpretation of the solutions is completely different from the point particle case. In particular, a null string is generally not propagating in a plane through the origin, although each of its individual points is. Some special solutions are obtained and their physical interpretation is given. Especially, the solution for a null string with a constant radial coordinate $r$ moving vertically from the south pole to the north pole around the photon sphere, is presented. A general discussion of classical null/tensile strings as compared to massless/massive particles is given. For instance, tensile circular solutions with a constant radial coordinate $r$ do not exist at all. The results are discussed in relation to the previous literature on the subject.Comment: 16 pages, REVTEX, no figure

Discovery and quantitative spectral analysis of an Ofpe/WN9 (WN11) star in the Sculptor spiral galaxy NGC 300

We have discovered an Ofpe/WN9 (WN11 following Smith et al.) star in the Sculptor spiral galaxy NGC 300, the first object of this class found outside the Local Group, during a recent spectroscopic survey of blue supergiant stars obtained at the ESO VLT. The light curve over a five-month period in late 1999 displays a variability at the 0.1 mag level. The intermediate resolution spectra (3800-7200 A) show a very close resemblance to the Galactic LBV AG Car during minimum. We have performed a detailed non-LTE analysis of the stellar spectrum, and have derived a chemical abundance pattern which includes H, He, C, N, O, Al, Si and Fe, in addition to the stellar and wind parameters. The derived stellar properties and the He and N surface enrichments are consistent with those of other Local Group WN11 stars in the literature, suggesting a similar quiescent or post-LBV evolutionary status.Comment: 9 pages, 4 figures, 2 tables. Accepted for publication in the Astrophysical Journal Letter

New rotation periods in the Pleiades: Interpreting activity indicators

We present results of photometric monitoring campaigns of G, K and M dwarfs in the Pleiades carried out in 1994, 1995 and 1996. We have determined rotation periods for 18 stars in this cluster. In this paper, we examine the validity of using observables such as X-ray activity and amplitude of photometric variations as indicators of angular momentum loss. We report the discovery of cool, slow rotators with high amplitudes of variation. This contradicts previous conclusions about the use of amplitudes as an alternate diagnostic of the saturation of angular momentum loss. We show that the X-ray data can be used as observational indicators of mass-dependent saturation in the angular momentum loss proposed on theoretical grounds.Comment: 24 pages, LaTex (AASTeX); includes 8 postscript figures and 4 Latex tables. To appear in ApJ, Feb. 1, 1998. Postscript version of preprint can be obtained from http://casa.colorado.edu/~anitak/pubs.htm

USp(2k) Matrix Model: Nonperturbative Approach to Orientifolds

We discuss theoretical implications of the large k USp(2k) matrix model in zero dimension. The model appears as the matrix model of type IIB superstrings on a large $T^{6}/Z^{2}$ orientifold via the matrix twist operation. In the small volume limit, the model behaves four dimensional and its T dual is six-dimensional worldvolume theory of type I superstrings in ten spacetime dimensions. Several theoretical considerations including the analysis on planar diagrams, the commutativity of the projectors with supersymmetries and the cancellation of gauge anomalies are given, providing us with the rationales for the choice of the Lie algebra and the field content. A few classical solutions are constructed which correspond to Dirichlet p-branes and some fluctuations are evaluated. The particular scaling limit with matrix T duality transformation is discussed which derives the F theory compactification on an elliptic fibered K3.Comment: LaTeX, 29 pages, 3 figures. PostScript problems are fixe

The central binary and surrounding nebula of the symbiotic star V1016 Cygni

We present HST/WFPC2 images of the symbiotic star V1016 Cyg which, for the first time, directly explore the inner regions of the nebula to AU scales at optical wavelengths. They also suggest that the [OIII] 4959,5007 emission takes place in a bipolar outflow. We use these images to determine the position of the two stars and hence a projected binary separation of 84 AU (assuming a distance of 2 kpc) and a position angle of 143.5 degrees. Furthermore, we combine our images with VLA radio imaging which enables diagnostic tests to be applied and properties of the circumstellar nebula to be determined. Finally we have obtained archive HST/STIS spectra of V1016 Cyg with which we were able to spatially resolve the source at various positions in the nebula. This enabled discovery of the ultraviolet counterpart to the ~25 arcsec extended emission found by Bang et al. (1992).Comment: Accepted for publication in Ap

Towards a tensionless string field theory for the N=(2,0) CFT in d=6

We describe progress in using the field theory of tensionless strings to arrive at a Lagrangian for the six-dimensional $\mathcal N=(2,0)$ conformal theory. We construct the free part of the theory and propose an ansatz for the cubic vertex in light-cone superspace. By requiring closure of the $(2,0)$ supersymmetry algebra, we fix the cubic vertex up to two parameters.Comment: 46 pages, 2 figures. V2: references added; minor changes and improvement