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Experimental reintroduction of northern wormwood (Artemisia campestris var. wormskioldii), a rare species of dynamic cobble bar environments on the Columbia River
Rare plant reintroductions that result in additional or more viable wild populations are important conservation tools for maintaining biodiverse ecosystems. Ideally, such projects are best designed as experiments, to improve biological and ecological knowledge of the selected species and monitoring long-term results. Northern wormwood (Artemisia campestris var. wormskioldii: Asteraceae), a nearly extinct, early seral species restricted to Columbia River riparian habitat, is only known from two native populations in Washington, set 300 river kilometers apart. Both populations are declining and show minimal recruitment. In cooperation with the U.S. Fish and Wildlife Service and Army Corps of Engineers, we set up an experimental reintroduction project with the objectives of: (1) investigating the effect of environmental factors on survival of northern wormwood, to gain a better understanding of this species' habitat requirements; and (2) creating a second viable population to further recovery objectives. To achieve these goals, we obtained seeds from multiple sources in order to increase the diversity of the founding population and determine germination rates for each source. We then planted 2,100 greenhouse-grown plants on Rufus Island, Oregon, in October, 2011. We transplanted 1,450 of these in experimental plots to examine the impacts of three environmental factors: (1) substrate type; (2) distance from the water line; and (3) presence or absence of the invasive shrub false indigo (Amorpha fruticosa). The remaining plants were placed in four surplus populations in order to increase the founding population size and create a viable population. Results showed that seeds germinated at different rates across sources and years. Success of transplants was measured by survivorship and reproductive output. Transplants were most successful when planted in sand substrate and at least nine meters from the water line. The presence of false indigo, a listed invasive species, did not have a significant effect on survival of transplants. Reproductive output showed that this population produced viable seeds and recruitment was observed by way of eleven seedlings. The results from this project present new information on northern wormwood habitat requirements, as well as provide a protocol for future recovery efforts
H2A.Z-Mediated Localization of Genes at the Nuclear Periphery Confers Epigenetic Memory of Previous Transcriptional State
Many genes are recruited to the nuclear periphery upon transcriptional activation. The mechanism and functional significance of this recruitment is unclear. We find that recruitment of the yeast INO1 and GAL1 genes to the nuclear periphery is rapid and independent of transcription. Surprisingly, these genes remain at the periphery for generations after they are repressed. Localization at the nuclear periphery serves as a form of memory of recent transcriptional activation, promoting reactivation. Previously expressed GAL1 at the nuclear periphery is activated much more rapidly than long-term repressed GAL1 in the nucleoplasm, even after six generations of repression. Localization of INO1 at the nuclear periphery is necessary and sufficient to promote more rapid activation. This form of transcriptional memory is chromatin based; the histone variant H2A.Z is incorporated into nucleosomes within the recently repressed INO1 promoter and is specifically required for rapid reactivation of both INO1 and GAL1. Furthermore, H2A.Z is required to retain INO1 at the nuclear periphery after repression. Therefore, H2A.Z-mediated localization of recently repressed genes at the nuclear periphery represents an epigenetic state that confers memory of transcriptional activation and promotes reactivation
Cdk Phosphorylation of a Nucleoporin Controls Localization of Active Genes through the Cell Cycle
The localization of active genes to the nuclear periphery is regulated through the cell cycle by Cdk1 phosphorylation of a single nuclear pore protein
Concurrent Detection of Circulating Minor Histocompatibility Antigen-Specific CD8+ T Cells in SCT Recipients by Combinatorial Encoding MHC Multimers
Allogeneic stem cell transplantation (SCT) is a potentially curative treatment for patients with hematologic malignancies. Its therapeutic effect is largely dependent on recognition of minor histocompatibility antigens (MiHA) by donor-derived CD8+ T cells. Therefore, monitoring of multiple MiHA-specific CD8+ T cell responses may prove to be valuable for evaluating the efficacy of allogeneic SCT. In this study, we investigated the use of the combinatorial encoding MHC multimer technique to simultaneously detect MiHA-specific CD8+ T cells in peripheral blood of SCT recipients. Feasibility of this approach was demonstrated by applying dual-color encoding MHC multimers for a set of 10 known MiHA. Interestingly, single staining using a fluorochrome- and Qdot-based five-color combination showed comparable results to dual-color staining for most MiHA-specific CD8+ T cell responses. In addition, we determined the potential value of combinatorial encoding MHC multimers in MiHA identification. Therefore, a set of 75 candidate MiHA peptides was predicted from polymorphic genes with a hematopoietic expression profile and further selected for high and intermediate binding affinity for HLA-A2. Screening of a large cohort of SCT recipients resulted in the detection of dual-color encoded CD8+ T cells following MHC multimer-based T cell enrichment and short ex vivo expansion. Interestingly, candidate MiHA-specific CD8+ T cell responses for LAG3 and TLR10 derived polymorphic peptides could be confirmed by genotyping of the respective SNPs. These findings demonstrate the potency of the combinatorial MHC multimer approach in the monitoring of CD8+ T cell responses to known and potential MiHA in limited amounts of peripheral blood from allogeneic SCT recipients
A Uniform Genomic Minor Histocompatibility Antigen Typing Methodology and Database Designed to Facilitate Clinical Applications
BACKGROUND: Minor Histocompatibility (H) antigen mismatches significantly influence the outcome of HLA-matched allogeneic stem cell transplantation. The molecular identification of human H antigens is increasing rapidly. In parallel, clinical application of minor H antigen typing has gained interest. So far, relevant and simple tools to analyze the minor H antigens in a quick and reliable way are lacking. METHODOLOGY AND FINDINGS: We developed a uniform PCR with sequence-specific primers (PCR-SSP) for 10 different autosomal minor H antigens and H-Y. This genomic minor H antigen typing methodology allows easy incorporation in the routine HLA typing procedures. DNA from previously typed EBV-LCL was used to validate the methodology. To facilitate easy interpretation for clinical purposes, a minor H database named dbMinor (http://www.lumc.nl/dbminor) was developed. Input of the minor H antigen typing results subsequently provides all relevant information for a given patient/donor pair and additional information on the putative graft-versus-host, graft-versus-tumor and host-versus-graft reactivities. SIGNIFICANCE: A simple, uniform and rapid methodology was developed enabling determination of minor H antigen genotypes of all currently identified minor H antigens. A dbMinor database was developed to interpret the genomic typing for its potential clinical relevance. The combination of the minor H antigen genomic typing methodology with the online dbMinor database and applications facilitates the clinical application of minor H antigens anti-tumor targets after stem cell transplantation
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