Technical Note: Could benzalkonium chloride be a suitable alternative to mercuric chloride for preservation of seawater samples?

Instrumental equipment unsuitable or unavailable for fieldwork as well as lack of ship space can necessitate the preservation of seawater samples prior to analysis in a shore-based laboratory. Mercuric chloride (HgCl2) is routinely used for such preservation, but its handling and subsequent disposal incur environmental risks and significant expense. There is therefore a strong motivation to find less hazardous alternatives. Benzalkonium chloride (BAC) has been used previously as microbial inhibitor for freshwater samples. Here, we assess the use of BAC for marine samples prior to the measurement of oxygen-to-argon (O2 / Ar) ratios, as used for the determination of biological net community production. BAC at a concentration of 50 mg dm−3 inhibited microbial activity for at least 3 days in samples tested with chlorophyll a (Chl a) concentrations up to 1 mg m−3. BAC concentrations of 100 and 200 mg dm−3 were no more effective than 50 mg dm−3 . With fewer risks to human health and the environment, and no requirement for expensive waste disposal, BAC could be a viable alternative to HgCl2 for short-term preservation of seawater samples, but is not a replacement for HgCl2 in the case of oxygen triple isotope analysis, which requires storage over weeks to months. In any event, further tests on a case-by-case basis should be undertaken if use of BAC was considered, since its inhibitory activity may depend on concentration and composition of the microbial community

From Graphene constrictions to single carbon chains

We present an atomic-resolution observation and analysis of graphene constrictions and ribbons with sub-nanometer width. Graphene membranes are studied by imaging side spherical aberration-corrected transmission electron microscopy at 80 kV. Holes are formed in the honeycomb-like structure due to radiation damage. As the holes grow and two holes approach each other, the hexagonal structure that lies between them narrows down. Transitions and deviations from the hexagonal structure in this graphene ribbon occur as its width shrinks below one nanometer. Some reconstructions, involving more pentagons and heptagons than hexagons, turn out to be surprisingly stable. Finally, single carbon atom chain bridges between graphene contacts are observed. The dynamics are observed in real time at atomic resolution with enough sensitivity to detect every carbon atom that remains stable for a sufficient amount of time. The carbon chains appear reproducibly and in various configurations from graphene bridges, between adsorbates, or at open edges and seem to represent one of the most stable configurations that a few-atomic carbon system accomodates in the presence of continuous energy input from the electron beam.Comment: 12 pages, 4 figure

Detecting the Earliest Galaxies Through Two New Sources of 21cm Fluctuations

The first galaxies that formed at a redshift ~20-30 emitted continuum photons with energies between the Lyman-alpha and Lyman limit wavelengths of hydrogen, to which the neutral universe was transparent except at the Lyman-series resonances. As these photons redshifted or scattered into the Lyman-alpha resonance they coupled the spin temperature of the 21cm transition of hydrogen to the gas temperature, allowing it to deviate from the microwave background temperature. We show that the fluctuations in the radiation emitted by the first galaxies produced strong fluctuations in the 21cm flux before the Lyman-alpha coupling became saturated. The fluctuations were caused by biased inhomogeneities in the density of galaxies, along with Poisson fluctuations in the number of galaxies. Observing the power-spectra of these two sources would probe the number density of the earliest galaxies and the typical mass of their host dark matter halos. The enhanced amplitude of the 21cm fluctuations from the era of Lyman-alpha coupling improves considerably the practical prospects for their detection.Comment: 11 pages, 7 figures, ApJ, published. Normalization fixed in top panels of Figures 4-

A modular approach to the antifungal sphingofungin family: concise total synthesis of sphingofungin A and C

Sphingofungins are fungal natural products known to inhibit the biosynthesis of sphingolipids which play pivotal roles in various cell functions. Here, we report a short and flexible synthetic approach towards the sphingofungin family. Key step of the synthesis was a decarboxylative cross-coupling reaction of chiral sulfinyl imines with a functionalized tartaric acid derivative, which yielded the core motive of sphingofungins carrying four consecutive stereocenters and a terminal double bond. Subsequent metathesis reaction allowed for the introduction of different side chains of choice resulting in a total of eight sphingofungins, including for the first time sphingofungin C (eight steps from commercially available protected tartaric acid with an overall yield of 6%) and sphingofungin A (ten steps). All newly synthesized derivatives were tested for their antifungal, cell proliferative and antiparasitic activity unraveling their structure-activity relations

Closed-loop and congestion control of the global carbon climate system

<jats:title>Abstract</jats:title><jats:p>The global carbon-climate system is a complex dynamical system with multiple feedbacks among components, and to steer this system away from dangerous climate change, it may not be enough to prescribe action according to long-term scenarios of fossil fuel emissions. We introduce here concepts from control theory, a branch of applied mathematics that is effective at steering complex dynamical systems to desired states, and distinguish between open- and closed-loop control. We attempt (1) to show that current scientific work on carbon-climate feedbacks and climate policy more closely resembles the conceptual model of open- than closed-loop control, (2) to introduce a mathematical generalization of the carbon-climate system as a compartmental dynamical system that can facilitate the formal treatment of the closed-loop control problem, and (3) to formulate carbon-climate control as a congestion control problem, discussing important concepts such as observability and controllability. We also show that most previous discussions on climate change mitigation and policy development have relied on an implicit assumption of open-loop control that does not consider frequent corrections due to deviations of goals from observations. Using a reduced complexity model, we illustrate that the problem of managing the global carbon cycle can be abstracted as a network congestion problem, accounting for nonlinear behavior and feedback from a global carbon monitoring system. As opposed to <jats:italic>scenarios</jats:italic>, the goal of closed-loop control is to develop <jats:italic>rules</jats:italic> for continuously steering the global carbon-climate system away from dangerous climate change.</jats:p&gt

A New Shear Estimator for Weak Lensing Observations

We present a new shear estimator for weak lensing observations which properly accounts for the effects of a realistic point spread function (PSF). Images of faint galaxies are subject to gravitational shearing followed by smearing with the instrumental and/or atmospheric PSF. We construct a `finite resolution shear operator' which when applied to an observed image has the same effect as a gravitational shear applied prior to smearing. This operator allows one to calibrate essentially any shear estimator. We then specialize to the case of weighted second moment shear estimators. We compute the shear polarizability which gives the response of an individual galaxy's polarization to a gravitational shear. We then compute the response of the population of galaxies, and thereby construct an optimal weighting scheme for combining shear estimates from galaxies of various shapes, luminosities and sizes. We define a figure of merit --- an inverse shear variance per unit solid angle --- which characterizes the quality of image data for shear measurement. The new method is tested with simulated image data. We discuss the correction for anisotropy of the PSF and propose a new technique involving measuring shapes from images which have been convolved with a re-circularizing PSF. We draw attention to a hitherto ignored noise related bias and show how this can be analyzed and corrected for. The analysis here draws heavily on the properties of real PSF's and we include as an appendix a brief review, highlighting those aspects which are relevant for weak lensing.Comment: 39 pages, 9 figure

Cooperative Scattering by Cold Atoms

We have studied the interplay between disorder and cooperative scattering for single scattering limit in the presence of a driving laser. Analytical results have been derived and we have observed cooperative scattering effects in a variety of experiments, ranging from thermal atoms in an optical dipole trap, atoms released from a dark MOT and atoms in a BEC, consistent with our theoretical predictions.Comment: submitted for special issue of PQE 201

Measuring the Reduced Shear

Neglecting the second order corrections in weak lensing measurements can lead to a few percent uncertainties on cosmic shears, and becomes more important for cluster lensing mass reconstructions. Existing methods which claim to measure the reduced shears are not necessarily accurate to the second order when a point spread function (PSF) is present. We show that the method of Zhang (2008) exactly measures the reduced shears at the second order level in the presence of PSF. A simple theorem is provided for further confirming our calculation, and for judging the accuracy of any shear measurement method at the second order based on its properties at the first order. The method of Zhang (2008) is well defined mathematically. It does not require assumptions on the morphologies of galaxies and the PSF. To reach a sub-percent level accuracy, the CCD pixel size is required to be not larger than 1/3 of the Full Width at Half Maximum (FWHM) of the PSF. Using a large ensemble (> 10^7) of mock galaxies of unrestricted morphologies, we find that contaminations to the shear signals from the noise of background photons can be removed in a well defined way because they are not correlated with the source shapes. The residual shear measurement errors due to background noise are consistent with zero at the sub-percent level even when the amplitude of such noise reaches about 1/10 of the source flux within the half-light radius of the source. This limit can in principle be extended further with a larger galaxy ensemble in our simulations. On the other hand, the source Poisson noise remains to be a cause of systematic errors. For a sub-percent level accuracy, our method requires the amplitude of the source Poisson noise to be less than 1/80 ~ 1/100 of the source flux within the half-light radius of the source, corresponding to collecting roughly 10^4 source photons.Comment: 18 pages, 3 figures, 4 tables, minor changes from the previous versio