Water use indicators at farm scale: Methodology and case study

Indicators for water use at farm scale can assist farmers in understanding the water flows on their farms and in optimizing water use by adapting agronomic measures and farm management. The objective of this work is to develop a methodology to estimate water flows at the farm scale, to derive indicators for farm water use, and to apply them in a first case study. After the spatial and temporal boundaries of the farm system and the water flows are defined, three indicators to assess water use at the farm scale are developed: farm water productivity, degree of water utilization, and specific inflow of technical water. Farm water productivity describes the ratio of farm output to water input, where the water input is the total of those water inflows into the farm system that can be assigned to the generation of farm output. Farm output is expressed on a mass basis, food energy basis, and monetary basis. The degree of water utilization characterizes the relationship between productive water to the total water inflow into the farm system, where productive water comprises those water flows that directly contribute to biomass generation via plant and animal metabolism. The specific technical water inflow quantifies the water inflow into the system by technical means relative to the farm area. The application of the methodology in a first case study for a mixed crop-livestock farm with 2869 ha in Germany results in a farm water productivity of 2.30 kg fresh mass per mWinput-3, 1.03 kg dry mass per m Winput-3, 5.96 GJ m Winput-3, and 0.25 € mWinput-3. The degree of water utilization is 0.56. The specific technical water inflow is 36.5 m3 ha-1 year -1. Factors that mainly effect these indicators and general approaches to optimize water use in farms are discussed as well as the further research required for practical implementation

Shear bands in granular flow through a mixing length model

We discuss the advantages and results of using a mixing-length, compressible model to account for shear banding behaviour in granular flow. We formulate a general approach based on two function of the solid fraction to be determined. Studying the vertical chute flow, we show that shear band thickness is always independent from flowrate in the quasistatic limit, for Coulomb wall boundary conditions. The effect of bin width is addressed using the functions developed by Pouliquen and coworkers, predicting a linear dependence of shear band thickness by channel width, while literature reports contrasting data. We also discuss the influence of wall roughness on shear bands. Through a Coulomb wall friction criterion we show that our model correctly predicts the effect of increasing wall roughness on the thickness of shear bands. Then a simple mixing-length approach to steady granular flows can be useful and representative of a number of original features of granular flow.Comment: submitted to EP

Water footprint analysis for the assessment of milk production in Brandenburg (Germany)

The working group "Adaptation to Climate Change" at the Leibniz-Institute for Agricultural Engineering Potsdam-Bornim (ATB) is introduced. This group calculates the water footprint for agricultural processes and farms, distinguished into green water footprint, blue water footprint, and dilution water footprint. The green and blue water demand of a dairy farm plays a pivotal role in the regional water balance. Considering already existing and forthcoming climate change effects there is a need to determine the water cycle in the field and in housing for process chain optimisation for the adaptation to an expected increasing water scarcity. Resulting investments to boost water productivity and to improve water use efficiency in milk production are two pathways to adapt to climate change effects. In this paper the calculation of blue water demand for dairy farming in Brandenburg (Germany) is presented. The water used for feeding, milk processing, and servicing of cows over the time period of ten years was assessed in our study. The preliminary results of the calculation of the direct blue water footprint shows a decreasing water demand in the dairy production from the year 1999 with 5.98×109 L/yr to a water demand of 5.00×109 L/yr in the year 2008 in Brandenburg because of decreasing animal numbers and an improved average milk yield per cow. Improved feeding practices and shifted breeding to greater-volume producing Holstein-Friesian cow allow the production of milk in a more water sustainable way. The mean blue water consumption for the production of 1 kg milk in the time period between 1999 to 2008 was 3.94±0.29 L. The main part of the consumed water seems to stem from indirect used green water for the production of feed for the cows

Prophage Spontaneous Activation Promotes DNA Release Enhancing Biofilm Formation in Streptococcus pneumoniae

Streptococcus pneumoniae (pneumococcus) is able to form biofilms in vivo and previous studies propose that pneumococcal biofilms play a relevant role both in colonization and infection. Additionally, pneumococci recovered from human infections are characterized by a high prevalence of lysogenic bacteriophages (phages) residing quiescently in their host chromosome. We investigated a possible link between lysogeny and biofilm formation. Considering that extracellular DNA (eDNA) is a key factor in the biofilm matrix, we reasoned that prophage spontaneous activation with the consequent bacterial host lysis could provide a source of eDNA, enhancing pneumococcal biofilm development. Monitoring biofilm growth of lysogenic and non-lysogenic pneumococcal strains indicated that phage-infected bacteria are more proficient at forming biofilms, that is their biofilms are characterized by a higher biomass and cell viability. The presence of phage particles throughout the lysogenic strains biofilm development implicated prophage spontaneous induction in this effect. Analysis of lysogens deficient for phage lysin and the bacterial major autolysin revealed that the absence of either lytic activity impaired biofilm development and the addition of DNA restored the ability of mutant strains to form robust biofilms. These findings establish that limited phage-mediated host lysis of a fraction of the bacterial population, due to spontaneous phage induction, constitutes an important source of eDNA for the S. pneumoniae biofilm matrix and that this localized release of eDNA favors biofilm formation by the remaining bacterial population

Novel biomaterials: plasma-enabled nanostructures and functions

Material processing techniques utilizing low-temperature plasmas as the main process tool feature many unique capabilities for the fabrication of various nanostructured materials. As compared with the neutral-gas based techniques and methods, the plasma-based approaches offer higher levels of energy and flux controllability, often leading to higher quality of the fabricated nanomaterials and sometimes to the synthesis of the hierarchical materials with interesting properties. Among others, nanoscale biomaterials attract significant attention due to their special properties towards the biological materials (proteins, enzymes), living cells and tissues. This review briefly examines various approaches based on the use of low-temperature plasma environments to fabricate nanoscale biomaterials exhibiting high biological activity, biological inertness for drug delivery system, and other features of the biomaterials make them highly attractive. In particular, we briefly discuss the plasma-assisted fabrication of gold and silicon nanoparticles for bio-applications; carbon nanoparticles for bioimaging and cancer therapy; carbon nanotube-based platforms for enzyme production and bacteria growth control, and other applications of low-temperature plasmas in the production of biologically-active materials

Rationalisation of the Differences between APOBEC3G Structures from Crystallography and NMR Studies by Molecular Dynamics Simulations

The human APOBEC3G (A3G) protein is a cellular polynucleotide cytidine deaminase that acts as a host restriction factor of retroviruses, including HIV-1 and various transposable elements. Recently, three NMR and two crystal structures of the catalytic deaminase domain of A3G have been reported, but these are in disagreement over the conformation of a terminal β-strand, β2, as well as the identification of a putative DNA binding site. We here report molecular dynamics simulations with all of the solved A3G catalytic domain structures, taking into account solubility enhancing mutations that were introduced during derivation of three out of the five structures. In the course of these simulations, we observed a general trend towards increased definition of the β2 strand for those structures that have a distorted starting conformation of β2. Solvent density maps around the protein as calculated from MD simulations indicated that this distortion is dependent on preferential hydration of residues within the β2 strand. We also demonstrate that the identification of a pre-defined DNA binding site is prevented by the inherent flexibility of loops that determine access to the deaminase catalytic core. We discuss the implications of our analyses for the as yet unresolved structure of the full-length A3G protein and its biological functions with regard to hypermutation of DNA

Model Structure of Human APOBEC3G

BACKGROUND: APOBEC3G (apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G) has antiretroviral activity associated with the hypermutation of viral DNA through cytosine deamination. APOBEC3G has two cytosine deaminase (CDA) domains; the catalytically inactive amino-terminal domain of APOBEC3G (N-CDA) carries the Vif interaction domain. There is no 3-D structure of APOBEC3G solved by X-ray or nuclear magnetic resonance. METHODOLOGY/PRINCIPAL FINDINGS: We predicted the structure of human APOBEC3G based on the crystal structure of APOBEC2. To assess the model structure, we evaluated 48 mutants of APOBEC3G N-CDA that identify novel variants altering ΔVif HIV-1 infectivity and packaging of APOBEC3G. Results indicated that the key residue D128 is exposed at the surface of the model, with a negative local electrostatic potential. Mutation D128K changes the sign of that local potential. In addition, two novel functionally relevant residues that result in defective APOBEC3G encapsidation, R122 and W127, cluster at the surface. CONCLUSIONS/SIGNIFICANCE: The structure model identifies a cluster of residues important for packaging of APOBEC3G into virions, and may serve to guide functional analysis of APOBEC3G