Physical disruption of intervertebral disc promotes cell clustering and a degenerative phenotype

© 2019, The Author(s). To test the hypothesis that physical disruption of an intervertebral disc disturbs cell-matrix binding, leading to cell clustering and increased expression of matrix degrading enzymes that contribute towards degenerative disc cell phenotype. Lumbar disc tissue was removed at surgery from 21 patients with disc herniation, 11 with disc degeneration, and 8 with adolescent scoliosis. 5 μm sections were examined with histology, and 30-µm sections by confocal microscopy. Antibodies were used against integrin α5beta1, matrix metalloproteinases (MMP) 1, MMP-3, caspase 3, and denatured collagen types I and II. Spatial associations were sought between cell clustering and various degenerative features. An additional, 11 non-herniated human discs were used to examine causality: half of each specimen was cultured in a manner that allowed free ‘unconstrained’ swelling (similar to a herniated disc in vivo), while the other half was cultured within a perspex ring that allowed ‘constrained’ swelling. Changes were monitored over 36 h using live-cell imaging. 1,9-Di-methyl methylene blue (DMMB) assay for glycosaminoglycan loss was carried out from tissue medium. Partially constrained specimens showed little swelling or cell movement in vitro. In contrast, unconstrained swelling significantly increased matrix distortion, glycosaminoglycan loss, exposure of integrin binding sites, expression of MMPs 1 and 3, and collagen denaturation. In the association studies, herniated disc specimens showed changes that resembled unconstrained swelling in vitro. In addition, they exhibited increased cell clustering, apoptosis, MMP expression, and collagen denaturation compared to ‘control’ discs. Results support our hypothesis. Further confirmation will require longitudinal animal experiments

Methods of isolation and identification of pathogenic and potential pathogenic bacteria from skins and tannery effluents

Currently there is no standard protocol available within the leather industry to isolate and identify pathogenic bacteria from hides, skins or tannery effluent. This study was therefore carried out to identify simple but effective methods for isolation and identification of bacterial pathogens from the effluent and skins during leather processing. Identification methods based on both phenotypic and genotypic characteristics were investigated. Bacillus cereus and Pseudomonas aeruginosa were used as indicator bacteria to evaluate the isolation and identification methods. Decontaminated calfskins were inoculated with a pure culture of the above mentioned bacterial species followed by a pre-tanning and chromium tanning processes. Effluent samples were collected and skins were swabbed at the end of each processing stage. Bacterial identification was carried out based on the phenotypic characteristics; such as colony appearance on selective solid media, cell morphology following a standard Gram-staining and spore staining techniques, and biochemical reactions, e.g., the ability of a bacterial species to ferment particular sugars and ability to produce certain enzymes. Additionally, an identification system based on bacterial phenotypic characteristics, known as Biolog® system was applied. A pulsed-filed gel electrophoresis (PFGE) method for bacterial DNA fingerprinting was also evaluated and used for the identification of the inoculated bacteria. The methods described in the study were found to be effective for the identification of pathogenic bacteria from skins and effluent

OPTIMASI PADAT TEBAR TERHADAP PERTUMBUHAN DAN KELANGSUNGAN HIDUP UDANG VANAME (Litopenaus Vannamei) DENGAN SISTEM RESIRKULASI

Penelitian ini  bertujuan untuk Mengetahui pertumbuhan dan sintasan udang vaname (Litopenaeus vannamei) yang dipelihara menggunakan air baku dari hasil reserkulasi. Metode pengambilan sampel dalam penelitian ini dilakukandengan cara acak untuk menjaga agar tidak ada organisme yang terlalu kecil atau besar yang terambil pada saat sampling atau dalam hal ini metode yang digunakan adalah metode eksperimental yakni menguji hasil penelitian dengan ragam uji ANOVA. Hasil penelitian ini menunjukkan bahwa penggunaan kepadatan yang berbeda (1500 ekor/wadah) menyebabkanPertumbuhan mutlak, Laju pertumbuhan, dan Sintasan pada udang vaname (Litopenaeus vannamei)   meningkat. Hal inidisebabkan karena rendahnya kepadatan diperlakuan A (1500 ekor) dibandingkan perlakuan B (2000 ekor) dan perlakuan C (2500 ekor), menyebabkan tidak terjadi persaingan ruang gerak dan tidak terjadi persaingan makanan sehingga dapat memacu pertumbuhan dan sintasan udang vannamei (Litopenaeus vannamei). Pada penelitian ini disarankan, jika untukmenambah kepadatannya diharuskan menggunakan tambahan aerasi dan kincir untuk menyuplay oksigen kewadah budidaya

Van Kampen's expansion approach in an opinion formation model

We analyze a simple opinion formation model consisting of two parties, A and B, and a group I, of undecided agents. We assume that the supporters of parties A and B do not interact among them, but only interact through the group I, and that there is a nonzero probability of a spontaneous change of opinion (A->I, B->I). From the master equation, and via van Kampen's Omega-expansion approach, we have obtained the "macroscopic" evolution equation, as well as the Fokker-Planck equation governing the fluctuations around the deterministic behavior. Within the same approach, we have also obtained information about the typical relaxation behavior of small perturbations.Comment: 17 pages, 6 figures, submited to Europ.Phys.J.