980 research outputs found
Modeling effects of L-type ca(2+) current and na(+)-ca(2+) exchanger on ca(2+) trigger flux in rabbit myocytes with realistic T-tubule geometries.
The transverse tubular system of rabbit ventricular myocytes consists of cell membrane invaginations (t-tubules) that are essential for efficient cardiac excitation-contraction coupling. In this study, we investigate how t-tubule micro-anatomy, L-type Ca(2+) channel (LCC) clustering, and allosteric activation of Na(+)/Ca(2+) exchanger by L-type Ca(2+) current affects intracellular Ca(2+) dynamics. Our model includes a realistic 3D geometry of a single t-tubule and its surrounding half-sarcomeres for rabbit ventricular myocytes. The effects of spatially distributed membrane ion-transporters (LCC, Na(+)/Ca(2+) exchanger, sarcolemmal Ca(2+) pump, and sarcolemmal Ca(2+) leak), and stationary and mobile Ca(2+) buffers (troponin C, ATP, calmodulin, and Fluo-3) are also considered. We used a coupled reaction-diffusion system to describe the spatio-temporal concentration profiles of free and buffered intracellular Ca(2+). We obtained parameters from voltage-clamp protocols of L-type Ca(2+) current and line-scan recordings of Ca(2+) concentration profiles in rabbit cells, in which the sarcoplasmic reticulum is disabled. Our model results agree with experimental measurements of global Ca(2+) transient in myocytes loaded with 50 μM Fluo-3. We found that local Ca(2+) concentrations within the cytosol and sub-sarcolemma, as well as the local trigger fluxes of Ca(2+) crossing the cell membrane, are sensitive to details of t-tubule micro-structure and membrane Ca(2+) flux distribution. The model additionally predicts that local Ca(2+) trigger fluxes are at least threefold to eightfold higher than the whole-cell Ca(2+) trigger flux. We found also that the activation of allosteric Ca(2+)-binding sites on the Na(+)/Ca(2+) exchanger could provide a mechanism for regulating global and local Ca(2+) trigger fluxes in vivo. Our studies indicate that improved structural and functional models could improve our understanding of the contributions of L-type and Na(+)/Ca(2+) exchanger fluxes to intracellular Ca(2+) dynamics
Evidence for multiband superconductivity in the heavy fermion compound UNi2Al3
Epitaxial thin films of the heavy fermion superconductor UNi2Al3 with
Tc{max}=0.98K were investigated. The transition temperature Tc depends on the
current direction which can be related to superconducting gaps opening at
different temperatures. Also the influence of the magnetic ordering at TN=5K on
R(T) is strongly anisotropic indicating different coupling between the magnetic
moments and itinerant charge carriers on the multi-sheeted Fermi surface. The
upper critical field Hc2(T) suggests an unconventional spin-singlet
superconducting state.Comment: 4 pages, 6 figures revised version: inset of fig. 2 changed, fig. 3
added accepted for pub. in Phys. Rev. Lett. (estimated 9/04
Adaptation of Deeplab V3+ for Damage Detection on Port Infrastructure Imagery
Regular inspection and maintenance of infrastructure facilities are crucial to ensure their functionality and safety for users. However, current inspection methods are labor-intensive and can vary depending on the inspector. To improve this process, modern sensor systems and machine learning algorithms can be deployed to detect defects based on rapidly acquired data, resulting in lower downtime. A quality-controlled processing chain allows to provide hence informed uncertainty assessments to inspection operators. In this study, we present several Deeplab V3+ models optimized to predict corroded segments of the quay wall at JadeWeserPort, Germany, which is a dataset from the 3D HydroMapper research project. Our models achieve generally high accuracy in detecting this damage type. Therefore, we examine the use of a Region Growing-based weakly supervised approach to efficiently extend our model to other common types in the future. This approach achieves about 90 % of the results compared to corresponding fully supervised networks, of which a ResNet-50 variant peaks at 55.6 % Intersection-over-Union regarding the test set's corrosion class
A solvable model for the diffusion and reaction of neurotransmitters in a synaptic junction
<p>Abstract</p> <p>Background</p> <p>The diffusion and reaction of the transmitter acetylcholine in neuromuscular junctions and the diffusion and binding of Ca<sup>2+ </sup>in the dyadic clefts of ventricular myocytes have been extensively modeled by Monte Carlo simulations and by finite-difference and finite-element solutions. However, an analytical solution that can serve as a benchmark for testing these numerical methods has been lacking.</p> <p>Result</p> <p>Here we present an analytical solution to a model for the diffusion and reaction of acetylcholine in a neuromuscular junction and for the diffusion and binding of Ca<sup>2+ </sup>in a dyadic cleft. Our model is similar to those previously solved numerically and our results are also qualitatively similar.</p> <p>Conclusion</p> <p>The analytical solution provides a unique benchmark for testing numerical methods and potentially provides a new avenue for modeling biochemical transport.</p
Quality of life and clinical characteristics of self-improving congenital ichthyosis within the disease spectrum of autosomal recessive congenital ichthyosis
Background Autosomal-recessive congenital ichthyosis (ARCI) is a heterogeneous group of ichthyoses presenting at birth. Self-improving congenital ichthyosis (SICI) is a subtype of ARCI and is diagnosed when skin condition improves remarkably (within years) after birth. So far, there are sparse data on SICI and quality of life (QoL) in this ARCI subtype. This study aims to further delineate the clinical spectrum of SICI as a rather unique subtype of ARCI. Objectives This prospective study included 78 patients (median age: 15 years) with ARCI who were subdivided in SICI (n = 18) and non-SICI patients (nSICI, n = 60) by their ARCI phenotype. Methods Quality of life (QoL) was assessed using the (Children's) Dermatology Life Quality Index. Statistical analysis was performed with chi-squared and t-Tests. Results The genetically confirmed SICI patients presented causative mutations in the following genes: ALOXE3 (8/16; 50.0%), ALOX12B (6/16; 37.5%), PNPLA1 (1/16; 6.3%) and CYP4F22 (1/16; 6.3%). Hypo-/anhidrosis and insufficient vitamin D levels (<30 ng/mL) were often seen in SICI patients. Brachydactyly (a shortening of the 4th and 5th fingers) was statistically more frequent in SICI (P = 0.023) than in nSICI patients. A kink of the ear's helix was seen in half of the SICI patients and tends to occur more frequently in patients with ALOX12B mutations (P = 0.005). QoL was less impaired in patients under the age of 16, regardless of ARCI type. Conclusions SICI is an underestimated, milder clinical variant of ARCI including distinct features such as brachydactyly and kinking of the ears. Clinical experts should be aware of these features when seeing neonates with a collodion membrane. SICI patients should be regularly checked for clinical parameters such as hypo-/anhidrosis or vitamin D levels and monitored for changes in quality of life
Flux Lattice Melting and Lowest Landau Level Fluctuations
We discuss the influence of lowest Landau level (LLL) fluctuations near
H_{c2}(T) on flux lattice melting in YBaCuO (YBCO). We
show that the specific heat step of the flux lattice melting transition in YBCO
single crystals can be attributed largely to the degrees of freedom associated
with LLL fluctuations. These degrees of freedom have already been shown to
account for most of the latent heat. We also show that these results are a
consequence of the correspondence between flux lattice melting and the onset of
LLL fluctuations.Comment: 4 pages, 2 embedded figure
Superconductivity in a layered cobalt oxyhydrate NaCoO1.3HO
We report the electrical, magnetic and thermal measurements on a layered
cobalt oxyhydrate NaCoO1.3HO. Bulk superconductivity
at 4.3 K has been confirmed, however, the measured superconducting fraction is
relatively low probably due to the sample's intrinsic two-dimensional
characteristic. The compound exhibits weak-coupled and extreme type-II
superconductivity with the average energy gap and the
Ginzburg-Landau parameter of 0.50 meV and 140,
respectively. The normalized electronic specific heat data in the
superconducting state well fit the dependence, suggesting point nodes
for the superconducting gap structure.Comment: 4 pages, 3 figure
Effect of alkali treatment of lower concentrations on the structure and tensile properties of Pakistan’s coarse cotton fibre
Cotton fibres of high Micronaire values are known to have inferior spinning performance. Either reduction of fibres’ fineness or increase in tensile strength is generally expected to improve the spinnability of fibres. In this piece of research, the effects of alkali treatment at lower concentrations (0.75–2.25M) and higher temperatures (70–100 °C) on the cross-section of cotton fibre and on the tensile strength have been investigated. Observations were made using scanning electron microscopy (SEM) and single fibre tensile strength testing. It was found that the roundness of the fibre cross section was improved and the tensile strength of the fibres also increased after treatment with alkali at lower concentration (0.75 M) and relatively lower temperature (70 °C). It is proposed that such changes occurred due to possible cellulose dissolution/transformations. It was thus concluded that the alkali treatment of cotton fibres at lower concentrations (0.75 M) and 70 °C for a shorter period of time (45 mins) could lead to improvement in tensile strength and roundness of fibre cross-section, thereby improving micronaire
Superconductivity in Cu_xTiSe_2
Charge density waves (CDWs) are periodic modulations of the conduction
electron density in solids. They are collective states that arise from
intrinsic instabilities often present in low dimensional electronic systems.
The layered dichalcogenides are the most well-studied examples, with TiSe_2 one
of the first CDW-bearing materials known. The competition between CDW and
superconducting collective electronic states at low temperatures has long been
held and explored, and yet no chemical system has been previously reported
where finely controlled chemical tuning allows this competition to be studied
in detail. Here we report how, upon controlled intercalation of TiSe_2 with Cu
to yield Cu_xTiSe_2, the CDW transition is continuously suppressed, and a new
superconducting state emerges near x = 0.04, with a maximum T_c of 4.15 K found
at x = 0.08. Cu_xTiSe_2 thus provides the first opportunity to study the CDW to
Superconductivity transition in detail through an easily-controllable chemical
parameter, and will provide new insights into the behavior of correlated
electron systems.Comment: Accepted to Nature Physic
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