Evaluation of binding mode between anticancer drug etoposide and human serum albumin by numerous spectrometric techniques and molecular docking

Etoposide (EPD) is a chemotherapy drug served to treat numerous cancers, and human serum albumin (HSA) functioning as transportation of variant small compounds (exogenous and endogenous). Its binding interaction was studied computationally (molecular docking) and spectrometrically by FT-IR, UV-vis, fluorescence (emission, 3D and synchronous) and CD with pH 7.40 at 292, 300 and 308 K temperatures. The obtained results were imparted that HSA bounded by EPD via static process, ultimately the formation of EPD-HSA complex with strong binding constant (10(5)) through vander Waals and hydrogen bonds. EPD can induce the differing in secondary structure, conformation and microenvironments of HSA upon binding; polarity around Trp increased and its distance to EPD was also analysed. Displacement estimations and molecular docking have demonstrated that EPD notably binds to site III (IB) of HSA. Influence of Co2+, Na+, Fe2+, Zn2+ and Ca2+ ions on EPD-HSA system was examined

Development and validation of a uv-spectrophotometric method for the quantitative determination of oxcarbazepine and study of its degradation profile

Oxcarbazepine (OXC) has attracted much attention owing to its multiple health benefits. OXC is an anticonvulsant and mood stabilizing drug, used primarily in the treatment of epilepsy. This study developed and validated a simple, specific, sensitive and reliable stability indicating UV-Spectrophotometric method for the quantitative determination of oxcrbazepine in pure and in pharmaceutical dosage forms. Analysis was performed using methanol: aceonitrile (50:50) as diluents. UV detection was performed at 254 nm. The calibration plot was linear over a concentration range of 0.0-15 µg ml-1 with correlation coefficient values >0.9986. The method was validated for specificity, linearity, precision, accuracy, limit of detection, limit of quantification and robustness. The forced degradation studies were performed by using HCl, NaOH, H2O2, thermal and UV radiation. Oxcarbazepine is more sensitive towards alkaline conditions and very much resistant towards acidic, oxidative and photolytic degradations. The method was validated as per ICH guidelines. The RSD for intra- and inter-day precision were found to be lesser than 3. The percentage recovery was in good agreement with the labeled amount in the pharmaceutical formulations and the method is simple, specific, precise and accurate for the determination of oxcarbazepine in pharmaceutical formulation

HPLC determination of ritodrine hydrochloride in pharmaceutical formulations

A simple, rapid, and accurate HPLC method is described for the determination of ritodrine hydrochloride (RTH) in both pure form and pharmaceutical formulations. A Hypersil Shendon ODS column with a mobile phase of dibasic phosphate buffer and acetonitrile (75 : 25) and isoxsuprine hydrochloride were used as an internal standard. The flow rate was 1 mL min--1 and the effluent was monitored at 270 nm pH 4.0. The calibration graph is linear in the range 2--30 μg mL--1. The proposed HPLC method has been successfully employed for the determination of RTH in Yutopar tablets and injection solutions

Synthesis and characterization of oxidovanadium(iv) complexes of 2-((e)-(6-fluorobenzo[d]thiazol-2-ylimino) methyl)-6-methoxyphenol and their antimicrobial, antioxidant, and DNA-binding studies

Two novel oxidovanadium(IV) complexes with a new bidentate (O- and N-) imine-based ligand 2-((E)-(6-fluorobenzo [d] thiazol-2-ylimino)methyl)-6-methoxyphenol (HL) were synthesized under in situ experimental condition where VOSO4 acts as a kinetic template in the ratio 2 : 1 (L : M) and mixed ligand complex using 1,10-phenanthroline (phen) in 1 : 1 : 1 (L : M : phen) ratio. The synthesized compounds were structurally characterized by microanalysis, magnetic susceptibility, FTIR, electronic spectra, TG/DTA, ESR, and molar conductance studies. Based on the spectral studies, the complexes have the general composition [VO(L)2] (C1) and [VO(L)phen] (C2) in a square pyramid geometrical fashion. The synthesized compounds were primarily screened for their in vitro growth inhibiting activity against different strains of bacteria, namely, E. coli, B. subtilis, S. aureus, and P. aeruginosa by the disc diffusion method. Also, the antifungal activity was determined against C. albicans and A. niger by the Bateman poisoned technique. The in vitro antioxidant activity of all the compounds was determined by DPPH free radical-scavenging assay. Intercalative mode of DNA-binding properties of the oxidovanadium(IV) complexes with calf-thymus DNA (CT-DNA) was investigated using UV, fluorescence spectra, and viscosity measurements

A Sensitive spectrophotometric determination of ritodrine, pentazocine, isoxsuprine hydrochlorides and amoxicillin in pure and pharmaceutical samples

A simple, accurate and highly sensitive spectrophotometric method for the determination of ritodrine hydrochloride (RTH), pentazocine hydrochloride (PZH), isoxsuprine hydrochloride (ISH) and amoxicillin (AMX) is described. The method is based on the oxidation of the studied drugs by a known excess of chloramine – T (CAT) in hydrochloric acid medium and subsequent determination of the unreacted oxidant by reacting it with iodide in the same acid medium liberates iodine, which subsequently react with starch to form a stable starch-iodine complex. The reacted oxidant corresponds to the drug content. The coloured complex exhibits a maximum absorption at 590 nm. The apparent molar absorptivity values and Sandell’s sensitivity values are in the range 6.96x104 - 1.43x105 L mol–1 cm–1 and 2.45-4.30 ng cm–2, respectively. The method was successfully applied to the studied drugs in their dosage forms. The results are reproducible within ±1% and compare favorably with those of official methods of British Pharmacopoeia and the United States Pharmacopoeia

2-{[(E)-2-Hy­droxy­benzyl­idene]amino}-1H-isoindole-1,3(2H)-dione

In the title compound, C15H10N2O3, the isoindoline ring system is almost planar [maximum deviation = 0.020 (2) Å] and makes a dihedral angle of 1.57 (7)° with the benzene ring. Intra­molecular O—H⋯N and C—H⋯O hydrogen bonds are observed

Bis[N,N-dimethyl-1-(10H-pyrido[3,2-b][1,4]benzothia­zin-10-yl)propan-2-aminium] tetrakis­(thio­cyanato-κN)cobaltate(II)

The asymmetric unit of the title salt, (C16H20N3S)2[Co(NCS)4], comprises one monovalent isothio­pendylium cation and one-half of a divalent thio­cyanatocobaltate(II) anion (2 symmetry). The central thia­zine ring of the cation is slightly twisted in a boat-like fashion, with r.m.s. deviations from the mean plane of 0.272 (1) and 0.2852 (8) Å for the N and S atoms. The mol­ecular structure of the cation is stabilized by an intra­molecular N—H⋯N hydrogen bond. Within the complex anion, the CoII atom is tetra­hedrally surrounded by four N atoms of the thio­cyanate ligands. π–π stacking, with a distance of 3.7615 (10) Å between the centroids of benzene and pyridine rings, helps to consolidate the packing

Spectrophotometric Determination of Mycophenolate Mofetil as Its Charge-Transfer Complexes with Two π-Acceptors

Two simple, selective, and rapid spectrophotometric methods are described for the determination of mycophenolate mofetil (MPM) in pure form and in tablets. Both methods are based on charge-transfer complexation reaction of MPM with p-chloranilic acid (p-CA) or 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) in dioxane-acetonitrile medium resulting in coloured product measurable at 520 nm (p-CA) or 580 nm (DDQ). Beer's law is obeyed over the concentration ranges of 40–400 and 12–120 μg mL−1 MPM for p-CA and DDQ, respectively, with correlation coefficients (r) of 0.9995 and 0.9947. The apparent molar absorptivity values are calculated to be 1.06 × 103 and 3.87 × 103 L mol−1 cm−1, respectively, and the corresponding Sandell's sensitivities are 0.4106 and 0.1119 μg cm−1. The limits of detection (LOD) and quantification (LOQ) are also reported for both methods. The described methods were successfully applied to the determination of MPM in tablets. Statistical comparison of the results with those of the reference method showed excellent agreement. No interference was observed from the common excipients present in tablets. Both methods were validated statistically for accuracy and precision. The accuracy and reliability of the methods were further ascertained by recovery studies via standard addition procedure

Antihyperlipidemic activity of Chloroxylon swietenia in triton WR1339 induced hyperlipidemia

Background: Medicinal herbs are beneficial and effective either in the management and prevention of several metabolic disorders, associated with hyperlipidemia, hypertension and insulin resistance which increases the cardio-metabolic risk and demands for the life time therapy. Current allopathic medicines are expensive and reported with several adverse effects and hence, finding of a suitable herbal medicine for hyperlipidemic disorders is very important.Methods: Thirty albino rats weighing 200-230g were randomly divided into 5 groups were rendered hyperlipidemia with a single dose of triton WR 1339. Normal control, positive control, standard, aqueous and ethanolic extract groups were treated with tween-80, tween-80, atorvastatin, aqueous and ethanolic extracts of Chloroxylon swietenia respectively for seven days. At the end of the study, blood was collected for estimation of the lipid profile.Results: Both the aqueous and ethanolic extract groups significantly reduced the TG and VLDL levels.Conclusions: The extracts exhibited remarkable activity on one or either parameter of the lipid profile. It could be due to the presence of alkaloids, steroids, flavonoids, coumarins and phenols in the extracts