Optimising the Analysis Stage in the Internationalisation of Manufacturing Operations

[EN] The internationalisation of the manufacturing operations process includes decision-making about new facility implementation (NFI) and global supplier network development (GSND), whose first step is to analyse the situation of a company and its environment. The purpose of this paper is to investigate the optimal design of a manufacturing production and distribution network for global small-and medium-sized enterprises (SMEs). This research uses a mixed-integer linear programming (MILP) model to support decision-making in the analysis stage of the internationalisation of manufacturing operations for global SMEs. A real-world case study is presented to illustrate the application of the proposed model. Different scenarios were evaluated not only to identify the strengths and limitations of the mathematical programming model, but to also provide support for the next strategic decisions that the examined company has to make in the near future.This work was supported by the Spanish Ministry of Science, Innovation and Universities project entitled `Optimisation of zero-defects production technologies enabling supply chains 4.0 (CADS4.0)' (RTI2018-101344-B-I00).Comer, F.; Mula, J.; Díaz-Madroñero Boluda, FM.; Grillo, H. (2021). Optimising the Analysis Stage in the Internationalisation of Manufacturing Operations. The South African Journal of Industrial Engineering. 32(2):124-132. https://doi.org/10.7166/32-2-2371S12413232

Metabolic engineering to simultaneously activate anthocyanin and proanthocyanidin biosynthetic pathways in Nicotiana spp

[EN] Proanthocyanidins (PAs), or condensed tannins, are powerful antioxidants that remove harmful free oxygen radicals from cells. To engineer the anthocyanin and proanthocyanidin biosynthetic pathways to de novo produce PAs in two Nicotiana species, we incorporated four transgenes to the plant chassis. We opted to perform a simultaneous transformation of the genes linked in a multigenic construct rather than classical breeding or retransformation approaches. We generated a GoldenBraid 2.0 multigenic construct containing two Antirrhinum majus transcription factors (AmRosea1 and AmDelila) to upregulate the anthocyanin pathway in combination with two Medicago truncatula genes (MtLAR and MtANR) to produce the enzymes that will derivate the biosynthetic pathway to PAs production. Transient and stable transformation of Nicotiana benthamiana and Nicotiana tabacum with the multigenic construct were respectively performed. Transient expression experiments in N. benthamiana showed the activation of the anthocyanin pathway producing a purple color in the agroinfiltrated leaves and also the effective production of 208.5 nmol (-) catechin/g FW and 228.5 nmol (-) epicatechin/g FW measured by the p-dimethylaminocinnamaldehyde (DMACA) method. The integration capacity of the four transgenes, their respective expression levels and their heritability in the second generation were analyzed in stably transformed N. tabacum plants. DMACA and phoroglucinolysis/HPLC-MS analyses corroborated the activation of both pathways and the effective production of PAs in T0 and T1 transgenic tobacco plants up to a maximum of 3.48 mg/g DW. The possible biotechnological applications of the GB2.0 multigenic approach in forage legumes to produce "bloatsafe" plants and to improve the efficiency of conversion of plant protein into animal protein (ruminal protein bypass) are discussed.This work was supported by grants BIO2012-39849-C02-01 and BIO2016-75485-R from the Spanish Ministry of Economy and Competitiveness (MINECO) (http://www.idi.mineco.gob.es/portal/site/MICINN) to LAC and a fellowship of the JAE-CSIC program to SF. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Fresquet-Corrales, S.; Roque Mesa, EM.; Sarrión-Perdigones, A.; Rochina, M.; López-Gresa, MP.; Díaz-Mula, HM.; Belles Albert, JM.... (2017). Metabolic engineering to simultaneously activate anthocyanin and proanthocyanidin biosynthetic pathways in Nicotiana spp. PLoS ONE. 12(9). https://doi.org/10.1371/journal.pone.0184839Se018483912

Nurses' perceptions of aids and obstacles to the provision of optimal end of life care in ICU

Contains fulltext : 172380.pdf (publisher's version ) (Open Access

Reduction of chilling injury and maintenance of fruit quality after pre- storage salicylic acid application on Iranian pomegranates

To cite th is article / Pou r citer cet article Redu ction of ch illin g in ju ry an d main ten an ce of fru it qu ality after pre-storage salicylic acid application on Iran ian pomegran ates

Postharvest biology and technology of plum

Games can provide an effective and replicable space in which stakeholders learn skills necessary for deliberative and pluralist policymaking. These skills are especially important for “nexus” policy issues that are typically characterised by multiple, competing problem frames involving overlapping networks of stakeholders. In this position paper, we describe three serious games that serve as a space for players (stakeholders) and researchers to jointly explore alternative solutions to complex resource management issues: the Water-Food-Energy Nexus Game (Nexus Game); the Narubu Game of Many Voices (Narubu Game); and the Forest Governance Game (Forest Game). The games contain instructive and reflexive mechanisms that prompt players to self-discover common challenges associated with complex nexus issues, including conflicting institutional mandates, social dilemmas, contending worldviews, and plural interpretations of science