1,395 research outputs found

    A Metataxonomic Approach Could Be Considered for Cattle Clinical Mastitis Diagnostics

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    Mastitis is one of the most costly diseases affecting the dairy industry, and identification of the causative microorganism(s) is essential. Here, we report the use of next-generation sequencing of bacterial 16S rRNA genes for clinical mastitis diagnosis. We used 65 paired milk samples, collected from the mastitic and a contralateral healthy quarter of mastitic dairy cattle to evaluate the technique as a potential alternative to bacterial culture or targeted PCR. One large commercial dairy farm was used, with one trained veterinarian collecting the milk samples. The 16S rRNA genes were individually amplified and sequenced using the MiSeq platform. The MiSeq Reporter was used in order to analyze the obtained sequences. Cattle were categorized according to whether or not 1 of the 10 most abundant bacterial genera in the mastitic quarter exhibited an increase in relative abundance between the healthy and mastitic quarters equal to, or exceeding, twofold. We suggest that this increase in relative abundance is indicative of the genus being a causative mastitis pathogen. Well-known mastitis-causing pathogens such as Streptococcus uberis and Staphylococcus spp. were identified in most cattle. We were able to diagnose 53 out of the 65 studied cases and identify potential new mastitis pathogens such as Sneathia sanguinegens and Listeria innocua, which are difficult to identify by bacterial culture because of their fastidious nature

    Photography as an act of collaboration

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    The camera is usually considered to be a passive tool under the control of the operator. This definition implicitly constrains how we use the medium, as well as how we look at – and what we see in – its interpretations of scenes, objects, events and ‘moments’. This text will suggest another way of thinking about – and using – the photographic medium. Based on the evidence of photographic practice (mine and others’), I will suggest that, as a result of the ways in which the medium interprets, juxtaposes and renders the elements in front of the lens, the camera is capable of depicting scenes, events and moments that did not exist and could not have existed until brought into being by the act of photographing them. Accordingly, I will propose that the affective power of many photographs is inseparable from their ‘photographicness’ – and that the photographic medium should therefore be considered as an active collaborator in the creation of uniquely photographic images

    Global citizenship as the completion of cosmopolitanism

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    A conception of global citizenship should not be viewed as separate from, or synonymous with, the cosmopolitan moral orientation, but as a primary component of it. Global citizenship is fundamentally concerned with individual moral requirements in the global frame. Such requirements, framed here as belonging to the category of individual cosmopolitanism, offer guidelines on right action in the context of global human community. They are complementary to the principles of moral cosmopolitanism – those to be used in assessing the justice of global institutions and practices – that have been emphasised by cosmopolitan political theorists. Considering principles of individual and moral cosmopolitanism together can help to provide greater clarity concerning individual duties in the absence of fully global institutions, as well as clarity on individual obligations of justice in relation to emerging and still-developing trans-state institutions

    Courage in decision-making:A mixed-methods study of COVID-19 vaccine uptake in women of reproductive age in the UK

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    COVID-19 vaccination rates are lower in women of reproductive age (WRA), including preg-nant/postpartum women despite their poorer COVID-19-related outcomes. We evaluated vac-cination experiences of 3,568 UK WRA, including 1,983 women (55.6%) experiencing a pandemic pregnancy, recruited through the ZOE COVID Symptom Study app. Two staggered online ques-tionnaires (Oct-Dec 2021: 3,453 responders; Aug-Sept 2022: 2,129 responders) assessed reproductive status, COVID-19 status, vaccination, and attitudes for/against vaccination. Descriptive analyses included vaccination type(s), timing relative to age-based eligibility and reproductive status, vaccination delay (first vaccination >28 days from eligibility), and rationale, with content analysis of free-text comments. Most responders (3,392/3,453, 98.2%) were vaccinated by Dec 2021, motivated by: altruism, vaccination supportiveness in general, low-risk, and COVID-19 concerns. Few declined vaccination (by Sept/2022: 20/2,129, 1.0%), citing: risks (pregnancy-specific and longer-term), pre-existing immunity, and personal/philosophical reasons. Few women de-layed vaccination, although pregnant/postpartum women (vs. other WRA), received vaccination later (median 3 vs. 0 days after eligibility, p<0.0001). Despite high uptake, concerns included: adverse effects; misinformation (including from healthcare providers); ever-changing govern-ment advice; and complex decision-making. In summary, most women in this large WRA cohort were promptly vaccinated, including pregnant/post-partum women. Altruism and community benefit superseded personal benefit as reasons for vaccination. Nevertheless, responders expe-rienced angst, and received vaccine-related misinformation and discouragement. These findings should inform vaccination strategies in WRA

    Conflict and Livelihood Decisions in the Chittagong Hill Tracts of Bangladesh

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    __Abstract__ We analyse rural household livelihood and child school enrolment decisions in the post-conflict setting of the Chittagong Hill Tracts (CHT) region of Bangladesh. What makes this paper innovative is the use of current subjective perceptions regarding the possibility of violence in the future and past actual experiences of violence in explaining household economic decision-making. Preferences are endogenous in line with behavioural economics. Regression results show that heightened subjective perceptions of future violence and past actual experiences of conflict influence current consumption and child enrolment and could encourage risky mixed crop cultivation. The trauma emanating from past experiences combined with current high perceptions of risk of violence may induce bolder and riskier behaviour in line with prospect theories of risk. Furthermore, a postconflic

    Learning capability : the effect of existing knowledge on learning

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    It has been observed that different people learn the same things in different ways - increasing their knowledge of the subject/domain uniquely. One plausible reason for this disparity in learning is the difference in the existing personal knowledge held in the particular area in which the knowledge increase happens. To understand this further, in this paper knowledge is modelled as a 'system of cognitive schemata', and knowledge increase as a process in this system; the effect of existing personal knowledge on knowledge increase is 'the Learning Capability'. Learning Capability is obtained in form of a function; although it is merely a representation making use of mathematical symbolism, not a calculable entity. The examination of the function tells us about the nature of learning capability. However, existing knowledge is only one factor affecting knowledge increase and thus one component of a more general model, which might additionally include talent, learning willingness, and attention

    The Citation Field of Evolutionary Economics

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    Evolutionary economics has developed into an academic field of its own, institutionalized around, amongst others, the Journal of Evolutionary Economics (JEE). This paper analyzes the way and extent to which evolutionary economics has become an interdisciplinary journal, as its aim was: a journal that is indispensable in the exchange of expert knowledge on topics and using approaches that relate naturally with it. Analyzing citation data for the relevant academic field for the Journal of Evolutionary Economics, we use insights from scientometrics and social network analysis to find that, indeed, the JEE is a central player in this interdisciplinary field aiming mostly at understanding technological and regional dynamics. It does not, however, link firmly with the natural sciences (including biology) nor to management sciences, entrepreneurship, and organization studies. Another journal that could be perceived to have evolutionary acumen, the Journal of Economic Issues, does relate to heterodox economics journals and is relatively more involved in discussing issues of firm and industry organization. The JEE seems most keen to develop theoretical insights

    A Complexity Architecture for Information Technologies: a Three-Year Didactic Experiment

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    One medium-term strategy for helping in the management of complexity is the introduction of a conceptual complexity component in the very centre of university curricula. In very few areas is the growth of complexity as evident as in the information technologies (ITs), the focus of the work presented in the current paper. We have therefore developed an integrated way of tackling the specific field of information technologies by means of an approach,to complexity. The content of this paper describes the guidelines of our research effort, placing an emphasis on informatics. Concepts of complexity based on the system metaphor have been substantially drawn upon in this exercise and are thus presented in some detail. Also described is a didactic experiment conducted by the author and designed to provide a new and integrating approach to University curricula for future professionals. The students' "discovery" of complexity is the focal point of the experiment. The findings of this effort are encouraging and call for the continuation and expansion of this experiment
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