Diplomatura Diseño y Gestión de Emprendimientos de Turismo Rural : estrategias de agregado de valor a la producción agropecuaria

Trabajos Finales de Integración y Transferencia, Cohorte Cerro Colorado (Diplomatura Diseño y Gestión de Emprendimientos de Turismo Rural) -- UNC- Facultad de Ciencias Agropecuarias, 2019En esta compilación se presentan una selección de cinco (5) trabajos realizados por los alumnos de la Cohorte de la Diplomatura desarrollada en la localidad de Cerro Colorado que involucra a proyectos en los Departamentos Río Seco, Sobremonte y Tulumba (Noroeste de la provincia de Córdoba, Argentina). El primer proyecto “Tulumba y sus sendas” trata de los diferentes circuitos turísticos en la Villa de Tulumba (Departamento Tulumba); el segundo, denominado “Vivencias camperas” propone vivenciar un día de campo en el establecimiento El Bordo situado en el Departamento Río Seco. El tercer proyecto plantea una “Raíz red turística” que comprende las localidades de Caminiaga, Cerro Colorado, Pozo Nuevo, San Francisco del Chañar y Villa María de Río Seco (Departamentos Río Seco y Sobremonte). “La huella de Don Ata”, en cuarto lugar, plantea un proyecto educativo relacionado al senderismo en la Comuna del Cerro Colorado. Finalmente, en “Soy el Mapa”, los autores proyectan el diseño de un cartel de interpretación ordenado en un solo punto que permita mostrar al turista los lugares relevantes del Cerro Colorado

Clínica jurídica de acción social

Memoria ID-025. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2018-2019

Clínica jurídica de acción social

Memoria ID-0032. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2017-2018

Effect of classical conditioning over immature and mature B lymphocytes regulation

© 2018 Wiley Periodicals, Inc. Classical conditioning (CC) with a sweet flavored is useful for experimental models of immune response. The objective this work was analyze spleen cell response on the differentiation of B-lymphocytes associated with the CC with sweet flavored in adult rats. Twelve adult male rats were divided (n = 3) in Group 1 (control); Group 2 without conditioned stimulus (WCS) received only water; Group 3 receiving only water with salt; Group 4 (experimental) with CS of sweet flavored and salty flavored afterwards. In every work group, all animals were subjected to the unconditioned stimulus (US) treated by 10 mg/kg body weight of cyclophosphamide injected intraperitoneally (i.p.). Subsequently an immunological challenge with sheep red blood cells i.p. was applied. Spleen samples were obtained 15 days after using euthanasia with 1% sodium thiopental i.p. The evaluation B lymphocyte (immature and mature) was performed by immunohistochemistry using specific antibodie

Ultrastructural preservation of tissues and their reaction to the infection with trichinella in the El Plomo mummy: muscle fiber ultrastructure and trichinosis/mummy of the Cerro El Plomo

The El Plomo mummy was a pre-Columbian Incan child who was found mummified in the Andes Mountains above an altitude of 17,700 feet. In the environment, natural mummification occurred due to low temperatures and strong winds. Dating measurements (relative dating) by experts from the National Museum of Natural History of Chile established that the mummified body corresponds the Inca period (1,450 to 1,500 AD). In 2003, the body was transferred to the University of Chile Medical School for exhaustive medical examination. Tissue samples from the right quadriceps muscle were extracted and fixed in glutaraldehyde and postfixed in osmium tetroxide to obtain ultrathin sections to be observed by transmission electron microscope. Images were recorded on photographic paper, digitalized and analyzed by experts on morphology. Results showed a preservation of cell boundaries in striated muscle cells, but specific subcellular organelles or contractile sarcomeric units (actin and myosin) were unable to be recognized. However, the classical ultrastructural morphology of the polypeptide collagen type I was preserved intact both in primary and secondary organization. Therefore, we concluded that the process of natural mummification by freezing and strong winds is capable of damaging the ultrastructure of muscle cells and preserving collagen type I intact.Dra. M. E. Castro Fondecyt Project 1101046 Proyecto Mayor Universidad de Tarapaca, Arica, Chile 4716-1

Efectos de cipermetrina sobre la expresión de citoqueratinas 8/18 y el receptor de andrógenos en la célula de sertoli del ratón adulto

Background: With the explosive population growth an increased use of land for cultivation purposes and the usage of biotechnologies in agriculture-such as pesticides-respond to the need for more efficient systems. However, improper application of pesticides has a negative effect on the environment, on exposed animals and on humans. Cypermethrin, a synthetic pyrethroid, is an insecticide with low risk to human and animal health and with broad insecticidal activity against a large number of pests. Studies in humans and animals show morphological and functional alterations in different organs exposed to cypermethrin. Pyrethroids are chemicals with structural similarity to pyrethrins and possess increased toxicity to insects over mammals. Objective: This research analyzes the variations of the state of differentiation of Sertoli cells and androgen receptor expression in testes of healthy adult mice exposed to cypermethrin. Material and method: Mice were divided into three groups: control 1 (untreated), control 2 (inoculated intraperitoneally with 0.1 ml of vegetable oil), and the experimental group 3 (inoculated with 1/5 of the lethal dose 50 (LD50 = 485 mg/kg) of cypermethrin). Results: Cypermethrin exerts acute and chronic effects on Sertoli cells in the testis of the adult mouse. These effects are manifested by the significant increase in epithelial height and the dedifferentiation of Sertoli cells evidenced through the presence of the Ck 8/18-type intermediate filament-a characteristic of differentiating cells-especially considering the functional cyclicity of the testicular compartment. Conclusions: Cypermethrin significantly affects the structure and function of Sertoli cells through the cytoskeleton and the state of maturation.Antecedentes: Con el crecimiento explosivo de la población, un mayor uso de la tierra con fines de cultivo y el uso de las biotecnologías en la agricultura----como pesticidas----responden a la necesidad de sistemas más eficientes. Sin embargo, la aplicación inadecuada de pesticidas tiene un efecto negativo sobre el medio ambiente, en los animales expuestos y en los seres humanos. La cipermetrina, un piretroide sintético, es un insecticida de bajo riesgo para la salud humana y animal, y con una amplia actividad insecticida frente a un gran número de plagas. Los estudios en seres humanos y animales muestran alteraciones morfológicas y funcionales en diferentes órganos expuestos a la cipermetrina. Los piretroides son sustancias químicas con estructura muy similar a las piretrinas, a menudo más tóxicas para insectos que para mamíferos. Objetivo: La presente investigación analiza las variaciones del estado de diferenciación de las células de Sertoli y de la expresión del receptor de andrógeno en testículos de ratones adultos sanos expuestos experimentalmente a cipermetrina. Material y método: Los animales fueron distribuidos en 3 grupos: control 1 (n= 3) sin tratamiento, control 2 (n = 15) inoculados con 0,1 ml de aceite vegetal vía intraperitoneal, y el grupo 3 y experimental (n = 15) inoculados con 1/5 de la dosis letal 50 (LD50= 485 mg/kg) de cipermetrina. Resultados: Se observó que la cipermetrina tiene efectos agudos y crónicos sobre las células de Sertoli en el testículo de ratón adulto. Estos efectos se demuestran por el aumento significativo de la altura epitelial, como también por una desdiferenciación de las células de Sertoli a través de la presencia de los filamentos intermedios tipo CK8/18, característico de células en diferenciación, más aun considerando la ciclicidad funcional del compartimiento testicular. Conclusiones: La cipermetrina afecta significativamente a la estructura y la funcionalidad de las células de Sertoli, a través del citoesqueleto y el estado de maduración.Proyecto UTA Mayor 4712-1

Effect of a single doses intraperitoneal of cypermethrin in brain somatosensory area of mice CF-1 Efecto de dosis única intraperitoneal de cipermetrina en la corteza cerebral somatosensorial de ratones CF- 1

The cypermethrin is a pesticide widely used in agriculture and public health. Their histopathological effects at central nervous system level, little have been studied, even though has demonstrated cases of poisoning in humans with important neurological commitment. The aim of this work is to know the effects a intraperitoneal single dose of cypermethrin on neuronal morphology of somatosensory cortex (superficial and deep laminae) of mice CF-1. 35 mice were separated in three groups: control (n=5), control vehicle (n=15) and experimental (n=15); the two last sacrificed groups 1, 8, 18, 26 and 35 days, three animals per group. The brain were removed, fixed, and process with routine technique, for a morphometric analysis. Statistical analysis with Kruskal Wallís test. We demonstrated corresponding morphologic alterations until death neuronal, in all studied laminae, at the different time intervals

Cambios Morfológicos de la Celularidad en la Glándula Prostática de Pacientes con Cáncer Confirmado: Nivel de Gleason y Presencia de Eosinófilos y Mastocitos: Bioindicadores Celulares

The different pathologies of the prostate, involve the presence of a new microenvironment where inflammatory cells are actively recruited. This research explores the presence of mast cells and eosinophils associated with age and the evaluation of prostate cancer progress (Gleason Index). Forty two biopsies of anonymized patients, with confirmed prostate cancer, were used for histological analysis for eosinophils and mast cells and subsequent determination of Gleason index according to age. The results of the histological analyzes show the presence of eosinophils and mast cells in prostate biopsies with confirmed cancer. In the multiple correlation studies, a high correlation was observed between the presence of lymphocytes and the age of the patient diagnosed with prostate cancer, same correlation was observed between the patient's age and higher Gleason Index (Pearson and Spearman p<0.05). It is concluded that in prostate biopsies from Chilean patients with confirmed cancer, eosinophilia and tissue mastocytosis were observed. Correlation analyzes show a direct correlation between older patients, higher Gleason index and presence of mast cell. Regarding eosinophilia, only a correlation between age and Gleason index was observed Further studies are suggested to determine that the presence of eosinophils and mast cells can be used as early bioindicators of prostate cancer.Project UTA, Universidad de Tarapaca, Arica-Chile 4716-1